아침 식사시간의 다른 사람들에서 혈장 그렐린의 변화

이상엽,김윤진,김형회,손한철,전태용,심문섭 대한비만학회 2002 The Korean journal of obesity Vol.11 No.2

연구배경 : 최근 식욕조절인자로 관심이 집중되고 있는 그렐린(ghrelin)은 식사 전후로 독한 일중변동을 보인다. 하지만, 지금까지는 실험적으로 일정한 열량의 표준 음식을 일정한 식사시간에 공급한 이후 그렐린의 농도 변화를 관찰하였다. 저자 등은 평소 아침 식사시간이 각기 다른 사람들에서 아침 식사 전후의 혈장 그렐린 농도 변화를 관찰하여 향후 외래 환경에서도 그렐린 관련 연구가 가능하도록 기초 자료를 제공하고자 하였다. 방법 : 연구에 대한 설명을 듣고 동의한 23.4 ~ 35.5세 사이의 비교적 건강한 동양인 남자 4명을 대상으로 하였다. 신체 계측을 한 후 이중 에너지 방사선 측정법 (Lunar prodigy, GE medical systems, Waukesha, Wisconsis, USA, 이하 DEXA)으로 체지방을 측정하였다. 연구 대상자 모두 평소 아침 식사시간이 일정하였지만 연구 시작 2주전부터 아침 식사시간을 엄격히 고정하도록 하였다. 스트레스가 없는 상태에서 아침 식사를 하지 않는 지원자는 오전 6시 30분부터, 나머지는 아침식사 시간 1시간 전부터 1시간 간격으로 점심 식사 전인 오전 11시 30분까지 채혈하였다. 각각의 검체로 부터 혈장 그렐린은 상업적인 방사선면역측정법 (Phoenix Pharmaceuticals, Inc., Belmont, CA)으로 측정되었다. 랩틴은 Ⅰ-125 표지 랩틴을 이용한 이중항체 방사선면역측정법으로, 혈장 인슐린은 항체 부착관을 이용한 방사선면역측정법으로, 혈당은 포도당산화 효소법에 의해 Synchron LX 20 (Beckman Coulter, Inc, Fullerton, USA)으로 측정하였다. 결과 : 연구 대상자의 체질량지수는 22.9 ~ 27.1 kg/㎡이었고, 허리둘레는 80.3 ~ 93.3 cm이었다. DEXA로 측정한 결과 전체 체지방과 체부지방 비율은 각각 27.1 ~ 31.8%와 32.7 ~ 32.4%이었다. 아침 식사를 하지 않는 자를 제외한 나머지 연구 대상자의 아침 식사 직전의 식후 2시간의 혈장 그렐린 농도는 각각 113.0 ~ 800.0 pg/mL, 78.3 ~ 553.0 pg/mL이었고 랩틴 농도는 각각 4.9 ~ 5.1 ng/mL, 4.4 ~ 4.7 ng/mL 이었다. 혈장 그렐린 랩틴 농도는 아침 식사 직전에 비해 식사 2시간 후 각각 7.2 ~ 30.9%와 7.8 ~ 10.2%감소되었다. 아침식사를 하지 않는 대상자의 경우 인슐린과 혈당치가 변화가 없음에도 불구하고 혈장 그렐린 농도는 오전 7시 30분에 가장 낮았다. 그 외 연구 대상자에서는 각기 다른 시간이더라도 아침 식사 2시간 후의 혈장 그렐린 농도가 가장 낮았다. 결론 : 평소 아침 식사시간이 다른 사람들에게서 혈장 그렐린 농도는 각기 다른 아침 식사 2 시간 후에 가장 낮았다. 아침 식사를 하지 않는 경우에는 혈장 그렐린 농도가 오전 7시 30분에 가장 낮았다. Background : Recently, the particular interest is on ghrelin, the dietary control factor among many scientists and it a toxic diurnal variations has been demonstrated before and after meal. However, the experimental approach has been only to see the changes in the concentration of ghrelin after intake of meals standardized with fixed calories at scheduled meal hours. the authors of this particular experiment have tried to observe and record the changes in concentrations of plasma ghrelin of persons with different breakfast hours. This might help in providing a basis for further possible studies in outpatient setting. Method : A group of four relatively healthy males whose ages between 23.4 and 35.5 with prior agreements were selected for this study. After body measurements, body lipid status was measured based on Lunar prodigy (GE medical systems, Waukesha, Wisconsin, USA) which is also referred to as DEXA. All of the selected persons had somewhat fixed breakfast time; however, they were asked to strictly keep their breakfast time fixed and steady starting two weeks before the beginning of experiment. The bloods of those who skip their breakfast without any particular stress were sampled at 6:30 AM, whereas the rest had different schedule, whose bloods were sampled every hour starting 1 hour before the first meal of the day till just before lunch (11:30 Am). From each blood sample, the level of plasma ghrelin was measured using the commercial radioimmune assay (Phoenix Pharmaceuticals, Inc., Belmont, CA). Leptin was measured with double antibody radioimmune assay using Ⅰ-125 labelled leptin, plasma insulin with radioimmune assay using antibody attachment tube, and blood sugar with Synchron LX20 (Beckman Coulter, Inc, Fullerton, USA) using glucosylation enzyme method. Result : The body mass index of the volunteers was 22.9 ~ 27.1 kg/㎡, with 80.3 ~ 93.3 cm waist circumference. Based on measurements by DEXA, the rates of total body lipid and trunk lipid were each 27.1 ~ 31.8% and 32.7 ~ 32.4%, respectively. The concentrations of plasma ghrelin of those who consume their breakfast before and 2 hour after their breakfast are 113.0 ~ 800.0 pg/mL and 78.3 ~ 553.0 pg/mL. The concentrations of leptin are 4.9 ~ 5.1 ng/mL and 4.4 ~ 4.7 ng/mL. Compared to the concentration of plasma ghrelin and of leptin recorded just before breakfast, it showed 7.2 ~ 30.9% and 7.8 ~ 10.2% decrease, respectively, 2 hours after breakfast. For those who skip their breakfast, the plasma concentration of ghrelin was recorded the lowest at 7:30 AM, even though there was no change in insulin and blood sugar. The rest of the subjects had their lowest plasma ghrelin concentration at 2 hours after breakfast, despite their different meal schedule. Conclusion : The persons with different breakfast hours had their lowest plasma concentration of ghrelin at 2 hours after breakfast. In contrast, the persons who skip their breakfast had their lowest concentration at 7: 30 AM.

중년의 과체중 환자에서 체중 변화가 간기능에 미치는 영향

이상엽,최상한,김윤진 대한비만학회 2002 The Korean journal of obesity Vol.11 No.4

연구배경 : 과체중 및 비만은 간기능 이상 및 지방간의 원인을 잘 알려져 있다. 본 연구는 중년의 과체중 환자에서 체중 변화가 간기능에 미치는 영향을 알아보고자 하였다. 방법 : 연구대상자는 평균 49.3(±6.9)세의 체질량지수 23kg/㎡ 이상인 57명에 국한하였다. Aspartate aminotransferase (AST)와 alanine aminotransferase (ALT)에 체중 이외에 영향을 미칠 수 이는 질환이나 조건을 배제하였다. 27개월 후 간기능 및 간초음파 검사로 추적관찰이 완료된 57명을 대상으로 첫 방문과 추적 방문 사이의 체중 변화와 간효소 수치의 변화를 비교 및 평가하였다. 결과 : 추적 관찰 기간에 3%의 체중감량을 보인 1군(40명)과 그렇지 않은 2군 (17명)을 비교하였다. 1군의 경우 aspartate aminotransfeerase (AST)와 alanine aminotransferase (ALT) 수치의 변화를 보이지 않은 반면, 2군에서는 처음 방문시보다 추적 방문시 통계학적으로 유의하게 더 높은 AST와 ALT 수치를 보였는데, 각각 5.2±7.1 IU/L 와 6.5±9.9 IU/L 증가하였다. 결론 : 중년의 과체중 환자에서 약 2년 동안 3% 이상 체중 감량이 이루어지지 않을 때, AST 및 ALT 수치가 처음보다 증가한다. Background : This study was aimed at finding out whether weight reduction alone can improve liver function in overweight or obese patients. Methods : We did a longitudinal, observational study on weight reduction. The study subjects were 57 patients referred to an obesity clinic in whom overweight or obesity is the sole factor causing abnormal liver function and fatty liver. Patients were weighed about 27 months later. Results : We compared the degree of improvement in hepatic function between Group I that showed weight reduction and Group Ⅱ that showed no-weight reduction. Group I (40) showed no remarkable change in aspartate aminotransferase (AST) and alanine aminotransferase(ALT) levels. But, Group Ⅱ(12) showed higher AST and ALT levels on follow-up visit than initial visit. AST showed statistically significant elevation by 5.2±7.1 IU/L. ALT also showed statistically significant elevation by 6.5±9.9 IU/L Conclusion : Without weight reduction above 3% during 27 months, overweight patients will have higher elevated AST or ALT than initial visit.

여성에서 폐경에 따른 요중 deoxypyridinoline과 혈청 osteocalcin의 변화

최상한,이상엽,김윤진 大韓家庭醫學會 2000 Korean Journal of Family Medicine Vol.21 No.12

비만 관련인자와 ALT 증가와의 관련성

민홍기,이정규,이하린,박현지,김윤진,이상엽 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

A Case of Scrub Typhus Complicated by Acute Calculous Cholecystitis

Lee, Su Jin,Cho, Young Hye,Lee, Sang Yeoup,Jeong, Dong Wook,Choi, Eun Jung,Kim, Yun Jin,Lee, Jeong Gyu,Lee, Yu Hyun The Korean Academy of Family Medicine 2012 Korean Journal of Family Medicine Vol.33 No.4

<P>We report a case of acute calculous cholecystitis through scrub typhus. A 69-year-old woman presented with a history of general myalgia, fever, and right abdominal pain. She referred to our hospital for surgical treatment of clinically suspected acute cholecystitis. Physicians concluded the cause of cholecystitis as gall bladder (GB) stone and proper antibiotics treatment of scrub typhus was started later. The patient developed acute respiratory distress syndrome and multi organ failure through scrub typhus. Five days after admission, the patient was treated with proper antibiotics and discharged on the 13th day after starting doxycycline treatment without any sequelae. In areas endemic for tsutsugamushi disease, even though a patient with GB stone presents with symptoms of acute cholecystitis, careful history and physical examination are required to reveal the existence of eschars or skin eruptions.</P>

Plasma Ghrelin and Peptide YY(3-36) Levels Change in Opposite Ways

( Sang Yeoup Lee ),( Hyoung Hoi Kim ),( Tae Yong Jeon ),( Yun Jin Kim ),( Sang Han Choi ),( Han Chul Son ),( Mun Sup Sim ) 대한비만학회 2004 대한비만학회 학술대회 Vol.- No.-

Site-directed mutagenesis substituting cysteine for serine in 2-Cys peroxiredoxin (2-Cys Prx A) of <i>Arabidopsis thaliana</i> effectively improves its peroxidase and chaperone functions

Lee, Eun Mi,Lee, Seung Sik,Tripathi, Bhumi Nath,Jung, Hyun Suk,Cao, Guang Ping,Lee, Yuno,Singh, Sudhir,Hong, Sung Hyun,Lee, Keun Woo,Lee, Sang Yeol,Cho, Jae-Young,Chung, Byung Yeoup Academic Press [etc.] 2015 Annals of botany Vol.116 No.4

<P><B>Background and Aims</B> The 2-Cys peroxiredoxin (Prx) A protein of <I>Arabidopsis thaliana</I> performs the dual functions of a peroxidase and a molecular chaperone depending on its conformation and the metabolic conditions. However, the precise mechanism responsible for the functional switching of 2-Cys Prx A is poorly known. This study examines various serine-to-cysteine substitutions on α-helix regions of 2-Cys Prx A in Arabidopsis mutants and the effects they have on the dual function of the protein.</P><P><B>Methods</B> Various mutants of 2-Cys Prx A were generated by replacing serine (Ser) with cysteine (Cys) at different locations by site-directed mutagenesis. The mutants were then over-expressed in <I>Escherichia coli</I>. The purified protein was further analysed by size exclusion chromatography, polyacrylamide gel electrophoresis, circular dichroism spectroscopy and transmission electron microscopy (TEM) and image analysis. Peroxidase activity, molecular chaperone activity and hydrophobicity of the proteins were also determined. Molecular modelling analysis was performed in order to demonstrate the relationship between mutation positions and switching of 2-Cys Prx A activity.</P><P><B>Key Results</B> Replacement of Ser<SUP>150</SUP> with Cys<SUP>150</SUP> led to a marked increase in holdase chaperone and peroxidase activities of 2-Cys Prx A, which was associated with a change in the structure of an important domain of the protein. Molecular modelling demonstrated the relationship between mutation positions and the switching of 2-Cys Prx A activity. Examination of the α<SUB>2</SUB> helix, dimer–dimer interface and C-term loop indicated that the peroxidase function is associated with a fully folded α<SUB>2</SUB> helix and easy formation of a stable reduced decamer, while a more flexible C-term loop makes the chaperone function less likely.</P><P><B>Conclusions</B> Substitution of Cys for Ser at amino acid location 150 of the α-helix of 2-Cys Prx A regulates/enhances the dual enzymatic functions of the 2-Cys Prx A protein. If confirmed <I>in planta</I>, this leads to the potential for it to be used to maximize the functional utility of 2-Cys Prx A protein for improved metabolic functions and stress resistance in plants.</P>

Effect of Supplements on Metabolic Syndrome

Sang Yeoup Lee 한국식품영양과학회 2018 한국식품영양과학회 학술대회발표집 Vol.2018 No.10

Enhancement of Chaperone Activity of Plant-Specific Thioredoxin through γ-Ray Mediated Conformational Change

Lee, Seung Sik,Jung, Hyun Suk,Park, Soo-Kwon,Lee, Eun Mi,Singh, Sudhir,Lee, Yuno,Lee, Kyun Oh,Lee, Sang Yeol,Chung, Byung Yeoup MDPI 2015 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol.16 No.11

<P>AtTDX, a thioredoxin-like plant-specific protein present in <I>Arabidospis</I> is a thermo-stable and multi-functional enzyme. This enzyme is known to act as a thioredoxin and as a molecular chaperone depending upon its oligomeric status. The present study examines the effects of γ-irradiation on the structural and functional changes of AtTDX. Holdase chaperone activity of AtTDX was increased and reached a maximum at 10 kGy of γ-irradiation and declined subsequently in a dose-dependent manner, together with no effect on foldase chaperone activity. However, thioredoxin activity decreased gradually with increasing irradiation. Electrophoresis and size exclusion chromatography analysis showed that AtTDX had a tendency to form high molecular weight (HMW) complexes after γ-irradiation and γ-ray-induced HMW complexes were tightly associated with a holdase chaperone activity. The hydrophobicity of AtTDX increased with an increase in irradiation dose till 20 kGy and thereafter decreased further. Analysis of the secondary structures of AtTDX using far UV-circular dichroism spectra revealed that the irradiation remarkably increased the exposure of β-sheets and random coils with a dramatic decrease in α-helices and turn elements in a dose-dependent manner. The data of the present study suggest that γ-irradiation may be a useful tool for increasing holdase chaperone activity without adversely affecting foldase chaperone activity of thioredoxin-like proteins.</P>

가정의학과 임상실습학생들의 논문작성법 교육에 적용한 간이 조사 시행 경험

이상엽(Sang-Yeoup Lee),김영주(Young-Joo Kim),최상한(Sang-Han Choi),이해균(Hae-Gyun Lee),안성률(Seong-Yul Ahn),김윤진(Yun-Jin Kim) 한국의학교육학회 2002 Korean journal of medical education Vol.14 No.1

Purpose: This study was to report our experience of the brief survey for teaching journal writing by medical students in a clinical curriculum of family medicine. Methods: Brief surveys performed by medical students in clinical clerkship of department of family medicine from 1998 to 2000. Medical students determined theme without rein for brief surveys. Lecture about meaning and process of survey was given for sufficient understanding of students, and then surveys were conducted. The subjects of survey were outpatients, nursing person, medical students, nursing students, and other general persons. The subjects of theme were classified to 17 chapters 7 components by international classification of primary care (ICPC). Results: In analysis of brief surveys according to ICPC, the reasons for surveys by components were diagnostic, screening prevention (89.0%), treatment, procedures, medication (5.7%), symptoms, complaints (5.3%). By chapters, there were social (64.6%), psychological (12.6%), metabolic and endocrine (6.5%). Among social problems, there were other social problem (35.4%), health care system/access (20.9%), problem with education (13.9%), relation problems partner (12.7%). Majority of medical students had affirmative response for survey conduction. In conclusion, our finding suggest that teaching journal writing as brief survey seems to be useful in understanding of journal writing of medical students.