1. Alcaligenes hydrogenophilus 에서 수소 산화능관련 유전자의 클로닝

민홍기 한국균학회 1986 韓國菌學會誌 Vol.14 No.4

肝吸蟲症의 肝癌誘發 可能性에 關한 硏究

閔弘基,韓雲燮 梨花女子大學校 醫科大學 醫科學硏究所 1983 EMJ (Ewha medical journal) Vol.6 No.3

The present study carried out to obtain further information on the relationship between primary carcinoma of the liver and infection with a liver-fluke, Clonorchis sinensis. A group of 50 male albino mice were used. Approximately 500 metacercariae were given orally to each mouse. Maturity of worms was followed up by fecal examination. Distribution of worms in the hepatobility system was also checked by using 5 egg positive mice at the end of the 1st month of infection. For histopathological examination, 4-5 mice were sacrificed monthly through the 9th month of infection. The liver tissues including common bile duct and the gall bladder were fixed in 10% formalin, sectioned serially, and stained with hematoxylin and eosin. The results obtained are summarized as follows; 1. The initial day of egg output was the 28th day in average ranging from 22nd to 33rd day of infection. 2. Recovery rate of worms was revealed as low as 16.8%, and of which 64.5% was found in the intrahepatic ducts, 33.2% in common bile duct and 2.3% in the gall bladder. 3. Parasites were found in the intrahepatic ducts throughout the observation. 4. At the end of the 1st month of infection, epithelial cells of bile ducts showed varying degrees of hyperplasia and desquamation, marked adenomatous tissue proliferation, enormous acini formation with remarkable mucinous cell metaplasia and definite atypia. Stratification was scanty. Periductal and portal spaces demonstrated the extensive chronic mononuclear leucocytes and the moderate fibrosis. Similar pictures persisted up to the 4th month of infection. 5. After the 5th month, all of the outstanding features of epithelial cells showed gradual regression. On the other hand, the most striking picture in this period was the great increase of collagen fibers in periductal and portal spaces. Although tumors were not found in the present study, overall results obtained were strongly suggestive to make an inference that clonorchiasis is responsible for the neoplastic transformation of epithelial cells of intrahepatic bile ducts in experimentally infected albino mice.

肝吸蟲感染 마우스의 膽管系 早期 變化

閔弘基,韓雲燮 梨花女子大學校 醫科大學 醫科學硏究所 1984 EMJ (Ewha medical journal) Vol.7 No.2

The objective of this sutdy was to obtain further information on the relation-ship between oncogenesis of cholangiocellular carcinoma of the liver and the infection with a liver fluke, Clonorchis sinensis in the earlier stage of the infec-tion. A group of 24 BALB/C male mice were used. Approximately 500 metacercariae were given orally to each mouse. Three mice were killed on the scheduled day, 3rd, 5th, 7th, 10th, 15th, 20th, 25th and 28th day after infection, respectively and histopathologic changes of the bile ducts were examined using hematoxylin and eosin stain method. The histopathologic findings observed were summarized as follows ; 1) Mice killed on the 3rd day showed a mild epithelial hyperplasia with the formation of papillae and adenomatous tissue proliferation. Bile ducts were dilated and the stroma was infiltrated by inflammatory cells, mainly eosinophils and polymorphonuclear leucocytes. Occasionally mitotic figures and atypia of epithelial cells were seen. Worms were found in the distal peripheral smaller branches of the bile duct. 2) Mice killed on the 7th day gave a mild connective tissue proliferation in addition to more striking pictures in the bile ducts. Chronic mononuclear leucocytes appeared in the stroma. 3) Bile duct chagnges including epithelial hyperplasia, adenomatous tissue for-mation, connective tissue proliferation and inflammatory cell infiltration were greater in the degrees in mice killed on the 10th day after infection. Mucinous cell metaplasia and collagen fiber proliferation were also observed. 4) From the 15th day throughout the 28th day after infection, mice showed the most striking pictures of peithelial hyperplasia, adenomatous tissue formation and mucinous cell metaplasia. The stroma demonstrated the excessive eosinophil and chronic mononuclear leucocyte infiltrations, while polymorphonuclear leucocytes were decreased.

재산세 과세대상 구분의 당위성 검토

민홍기 한국지방세연구원 2021 지방세포럼 Vol.57 No.-

Highly Sensitive and Stretchable Direction-Selective Crack-based Strain Sensor

민홍기,이화성 한국고분자학회 2015 한국고분자학회 학술대회 연구논문 초록집 Vol.2015 No.10

原發性肝癌患者에 있어서의 肝吸蟲感染의 疫學的 調査

민홍기,소진탁 梨花女子大學校 醫科大學 醫科學硏究所 1980 EMJ (Ewha medical journal) Vol.3 No.4

An epidemiological study on the primary carcinoma of the liver was carried out to obtain further information on the causal factor of cholangiocarcinoma and hepatocellular carcinoma during the period of 1997 and 1978. Total 102 hospital records of the primary carcinoma of the liver from 3 Hospitals were collected and analysed. Among 102 primary carcinomas of the liver, cases of hepatocellular carcinoma and cholangiocarcinoma were 96 (M83, F13) and 6 (M6, F1) respectively and the ratio was 16.0 : 1. Total cases of the primary carcinoma of the liver combined with clonorchiasis were 18 (M16, F2). Prevalence rate was 17.6 Clonorchiasis cases combined with hepatocllular carcinoma and cholangiocarcinoma were 14 (M13, F1) and 4 (M3, F1). Prevalence rate (66.7%) in cholangiocarcinoma was much higher than that (14.6%) in hepatocellular carcinoma. The relative risk was 11.8. Average ages of total cases, hepatocellular carcinoma and cholangiocarcinoma cases were 51.9, 51.5 and 58.5 respectively. Ages of clonorchiasis cases combined with hepatocellular carcinoma were distributed from 30 throughout 60 and those combined with cholangiocarcinoma were limited in 50 and 60. From the epidemiological point of view, the evidence for a carcinogenic effect of Clonorchis infection is strongly suggestive.

이슈진단 / 도선 , 도선사 그리고 한국해운

민홍기 한국해사문제연구소 1993 (月刊)海洋韓國 Vol.240 No.-

목표물질 스크리닝을 위한 피이크 인식 알고리즘

민홍기,홍승홍,Min, Hong-Kee,Hong, Seung-Hong 대한의용생체공학회 1993 의공학회지 Vol.14 No.2

In this paper, the peak detection algorithm was developed for the purpose of screening of the target compounds. Algorithm is divided into searching the characteristic ion and peak detection. The heuristic knowledge about analytical chemistry was applied for the searching the characteristic ion. Peak detection was accomplished in comparison with the peak identification strings and pattern strings around the retention time. Pattern strings are composed with the number which generated by pattern identification function. The variables of pattern identification function are the codes which represent the difference of two adjacent abundances Some of the free steroids were selected to demonstrate the proposed algorithm.

하이브리드 셋업을 이용한 에너지 효율적 센서 네트워크 클러스터링

민홍기,Min, Hong-Ki 한국융합신호처리학회 2011 융합신호처리학회 논문지 (JISPS) Vol.12 No.1

센서 네트워크에서 사용되는 동적 클러스터링 방식은 주기적으로 클러스터 구조가 바뀌는 셋업과정으로 인한 에너지 소모가 크다. 셋업과정은 보안적용을 해야 할 경우 보안 키가 주기적으로 재 생성되는 등 클러스터 구성 이외에 추가적인 에너지 낭비가 발생한다. 본 논문은 최초에 구성된 클러스터 알고리즘과 이후 반복적으로 발생되는 클러스터 재셋업 알고리즘을 달리하는 하이브리드 방식을 제안한다. 재 셋업에서는 고정된 클러스터 내에서 순환적으로 클러스터 헤드노드를 선출하는 순환적 클러스터 헤드선정(RRCH: Round-Robin Cluster Header)방식을 이용하여 에너지 소모를 줄인다. 보안키 생성 및 적용으로 추가되는 에너지 소모는 클러스터가 지속적으로 고정되기 때문에 최초 클러스터 형성 때 사전 배포하는 방식으로 해결된다. 본 논문에서 제안한 방식의 타당성을 확인하기 위해 모의실험을 실시하였다. 라운드 구간을 100번 반복하여 클러스터 구성과 데이터 전송을 포함한 전체 에너지 소모량을 측정하였다. 결과는 제안한 방식이 LEACH방식보다 평균 26.5%, HEED방식보다 평균 20% 적게 소모되는 것을 확인하였다. Cluster-based routing is high energy consumption of cluster head nodes. A recent approach to resolving the problem is the dynamic cluster technique that periodically re-selects cluster head nodes to distribute energy consumption of the sensor nodes. However, the dynamic clustering technique has a problem that repetitive construction of clustering consumes the more energies. This paper proposes a solution to the problems described above from the energy efficiency perspective. The round-robin cluster header(RRCH) technique, which fixes the initially structured cluster and sequentially selects cluster head nodes, is suggested for solving the energy consumption problem regarding repetitive cluster construction. A simulation result were compared with the performances of two of the most widely used conventional techniques, the LEACH(Low Energy Adaptive Clustering Hierarchy) and HEED(Hybrid, Energy Efficient, Distributed Clustering) algorithms, based on energy consumption, remaining energy for each node and uniform distribution. The evaluation confirmed that in terms of energy consumption, the technique proposed in this paper was 26.5% and 20% more efficient than LEACH and HEED, respectively.

사례연구 6 : 장애자가 있는 빈곤가정의 문제 연구

민홍기 어린이재단 (구 한국복지재단) 1990 사회복지관 사례연구 Vol.6 No.-