Development of a noninvasive KIM-1-based live-imaging technique in the context of a drug-induced kidney-injury mouse model

Tae-Jun Kwon,Da-Sol Lee,Md. Enamul Haque,Rang-Woon Park,Byungheon Lee,Dongkyu Kim,Yong-Hyun Jeon,Kil-Soo Kim,Sang Kyoon Kim 한국실험동물학회 2021 한국실험동물학회 학술발표대회 논문집 Vol.2021 No.7

The development of reliable methods to diagnose acute kidney injury is essential to allow the adoption of early therapeutic interventions and evaluate their effectiveness. Based on the fact that kidney injury molecule-1 (KIM-1) expression levels in kidneys are markedly upregulated early after a damage event, here we developed a noninvasive KIM- 1-based molecular imaging technique to detect kidney injury. First, we took advantage of a phage-display platform to select small peptides demonstrating a specific high binding affinity to KIM-1. The promising candidate was conjugated with fluorescent probes, and its imaging potential was validated in vitro and in vivo. This peptide, with the sequence CNRRRA, not only showed a high imaging potential in vitro, allowing a strong detection of KIM-1 expressing cells by microscopy and flow cytometry but also generated a strong kidney-specific signal in live-imaging in vivo experiments in the context of a drug-induced kidney-injury mouse model. Our data overall suggest that the CNRRRA peptide is a promising probe to use in the context of in vivo imaging for the detection of KIM-1 overexpression in damaged kidneys.

Lidocaine, Thrombin, Epinephrine 의 항균효과

김진우,이동건,전혜선,김승준,김석찬,안중현,김치홍,권순석,김영균,김관형,문화식,신완식,송정섭,박성학 대한감염학회 2005 감염과 화학요법 Vol.37 No.6

목적 : 기관지내시경 검사에서 흔히 사용되는 국소마취제인 lidocaine과 내시경시 지혈목적으로 사용되는 thrombin과 epinephrine이 각종 균주에 미치는 항균효과에 대해 알아보고자 하였다. 재료 및 방법 : 균주는 가톨릭대학교 성모병원에서 2004년 3월부터 2004년 9월까지 임상검체 에서 동정된 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 각각 42, 42, 42, 43주를 대상으로 하였다. Lidocaine, thrombin, epinephrine 감수성 검사는 National Committee for Clinical Laboratory Standards (NCCLS, 2002)의 기준에 따랐다. 결과 : Lidocaine은 S. aureus, S. pneumoniae, P. aeruginosa에서 MIC_(50), MIC_(90) 모두 20,000 ㎍/mL 이었다. K. pneumoniae는 각각 10,000 ㎍/mL이었다. Thrombin은 S. aureus와 P. aeruginosa에서 MIC50 500 lU/mL 과 MIC_(90) 500 IU/mL 이상이었고, K. pneumoniae에서는 MIC_(50)과 MIC_(90)이 모두 500 lU/mL이상이었으나 S. pneumoniae에서는 MIC_(50)과 MIC_(90)은 125 IU/mL이었다. Epinephrine은 K. pneumoniae, S. pneumoniae에 대한 MIC_(50), MIC_(90)가 모두 >500 ㎍/mL이었고, S. aureus와 P. aeruginosa에 대한 MIC_(50), MIC_(90)가 모두 500 ㎍/mL이었다. 결론 : 기관지 내시경 검사에서 흔히 쓰이는 lidocaine, thrombin, epinephrine 등의 약제들이 호흡기 질환의 흔한 병원균인 S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa 균주들에 대해 항균 효과가 있을 수 있어 세균배양검사에 영향을 미칠 수 있겠다. Background : We performed this stody to find out about antimicrobial effect of lidocaine which is commonly used local anesthetic, and thrombin and epinephrine used for hemostasis during bronchoscopic procedures. Materials and Methods : The microorganisms that were cultured from specimens obtained during bronchoscopy were Staphylococcus aureus (n=42), Streptococcus pneumoniae (n=42), Klebsiella pneumoniae (n=42), and Pseudomonas aeruginosa (n=43) collected from St. Mary's Hospital, from March to Sep 2004 were used for susceptibity testing. Susceptibility to lidocaine, thrombin, and epinephrine were tested according to the National Committee for Clinical Laboratory Standards. Result : MIC_(50) and MIC_(90) of lidocaine for S. aureus, S. pneumoniae, P. aeruginosa were all 20,000 ㎍/mL and that for K. pneumoniae were 10,000 ㎍/mL. MIC_(50) and MIC_(90) of thrombin for both S. aureus and P. aeruginosa was 500 IU/mL and above 500 IU/mL, respectively; that for K. pneumoniae were all above 500 IU/mL and for S. pneumoniae they were 125 IU/mL, MIC_(50) and MIC_(90) of epinephrine for K. pneumoniae and S. pneumoniae were above 500 ㎍/mL; that for S. aureus and P. aeruginosa were 500 ㎍/mL. Conclusion : We observed possible antimicrobial effect of lidocaine, thrombin, and epinephrine in vitro against pathogens such as S. aureus, S. pneumoniae, K. pneumoniae, P. aeruginosa, which are common respiratory microorganisms. The use of these agants could affect the result of bacterial culture.

야뇨증 환아들의 심리사회적 특성에 대한 다기관 연구 : 행동 및 정서 문제를 중심으로

조수철,김재원,신민섭,황준원,한상원,박관현,이상돈,김경도,김건석,서홍진,이유식,정재용,김영균,문두건,남궁미경,한창희,조원열,김영식,배기수,이종국,정우영,신의진 大韓神經精神醫學會 2005 신경정신의학 Vol.44 No.6

Objectives : The aim of this study was to examine the behavioral and emotional problems associated with nocturnal enuresis in Korean children. Methods : Three hundred eighteen children with nocturnal enuresis, together with their parents, completed the Child Behavior Checklist (CBCL), Disruptive Behavior Disorder Scale according to DSM-IV (DBDS), Children's Depression Inventory (CDI), State-Trait Anxiety Inventory for Children (STAIC), and Piers-Harris Children's Self-Concept Scale (PHCSC). Ninety-three normal students were selected as the control group. Results : Compared to the normal control group, the mean scores with regard to the withdrawn, social problems, attention problems, delinquent behavior, aggressive behavior, externalizing problems and total problems profiles were significantly higher in the nocturnal enuresis group according to the CBCL results. The nocturnal enuresis group also scored significantly higher in the ADHD and ODD profiles of the DBDS. The nocturnal enuresis group was more depressed and anxious than the control group according to the results of the CDI and STAI. The mean score of the PHCSC was significantly lower in the nocturnal enuresis group when compared to the normal control group. Conclusion : The results of this study suggest that children with nocturnal enuresis in Korea have clinically relevant behavioral and emotional problems. The findings support the link between nocturnal enuresis and psychopathology in Korean children.

Identification of a novel peptide that selectively binds to Kidney Injury Molecule-1 (KIM-1) by phage displayed peptide library

Tae-Jun Kwon,Joon-Suk Park,Sang-Hyun Kim,Enamul Haque,Byung-Heon Lee,Sang Eun Kim,Choong-Yong Kim,Sang Kyoon Kim 한국실험동물학회 2016 한국실험동물학회 학술발표대회 논문집 Vol.2016 No.2

칼라 영상 분할에 의한 차량번호판 영역 추출

김상균,박세명,최흥국,최항묵 인제대학교 1998 仁濟論叢 Vol.14 No.1

Extracting a license plate is an essential stage in the automatic car identification. Because car images are usually degraded and processes for the images are a computationally intensive job, it is difficult to extract a license plate correctly from the images. In this paper, we propose an extraction method of a license plate region based on color image segmentation. To segment the color image, we use a distributed genetic algorithm and transformed Hue-Saturation-Intensity color space as a measure of distance. The method offers robustness in dealing with deformation of car images and inherent parallelism to improve processing time. A test with car images on the roads shows an extraction rate of 93.8%. This result suggests that the proposed method works well with real-world situations, and is pertinent to be put into practical use.

간 질환시 동양주위세포의 알파-평활근 액틴 표출에 대한 면역조직 화학적 연구

김창진,강상균 순천향대학교 1994 논문집 Vol.17 No.4

The various liver diseases result in fibrosis, which is irreversible and pregressively replace normal hepatic parenchyma finally bringing out hepatic failure. Though It has not been elucidated which cells of the liver parenchyma are origin of hepatic fibrosis, perisinusoidal cells(PSCs) are thought to play a pivotal role in the pathogenesis of fibrocontractive changes in the late stage of various liver diseases. By light and electron microscopic immunohistochemistry, α-smooth muscle actin(α-SMA) is known to be the cytoskeletal marker of the fibrocontractibe cells either in granulation tissue of wound healing or in hepatic fibrosis. Several lines of evidence showed that the PSCs in fibrocontracted liver expressed the α-SMA strongly This study was done to evaluate the expression of α-SMA in various liver diseases The results were as follows; 1. Normal fetal liver showed mild α-SMA expression in periportal zone. The PSCs in intermediate zone and pervenulargions showed only negligible stain for α-SMA. 2. The chronic active hepatits revealed moderate α-SMA expression in periportal zone, especially in parenchymal necrotic region. The PSCs in intermediate zone and peribenular region showed mild α-SMA expression. 3. The chronic persistent hepatitis showed only mild α-SMA expression in periportal zone of mild inflammatory reaction. The intermediate zone and perivenular region showed negligible immunostain. 4. Alcoholic hepatitis showed strong α-SMA expression in perivenular region and the PSCs in intermediate zone also showed moderate immunoractibity. 5. The cirrhotic region after alcoholic hepatitis and chronic active hepatitis showed strong immunoreactibity ofr α-SMA in fibrous septa. The above result suggests that the mesenchymal cells expressing α-SMA as PSCs be responsible for the fibrosis of various liver diseases. As the expression of α-SMA precede the fibrocontraction of the liver, immunohistochemistry for α-SMA in various liver diseases would be necessory to predict the prognosis of the liver diseases.

위 장관 상피암에서 에스트로젠 수용체 표출에 대한 면역 조직학적 연구

김인건,김창진,강상균 순천향대학교 1992 논문집 Vol.14 No.4

The presence of Estrogen Receptor(ER) has been reported in various tumors, as orain tumor, lung cancer, gastrointestinal carcinomas and others, which have been believed to be non targer organs for the estrogen. The gastrointestinal carcinoma is one of the most frequent malignancy in Korea and several therapeutic modalities such as surgery, chemotherapy, radiotherapy and immunotherapy. are tried Hormone therapy had been applied selected cases with ER positive cancers and the results were good. To observe the presence of ER in gastrointestinal carcinomas We studied 20 cases of gastric carcinoma and 20 cases of colorectal carcinomas by immunohistochemical method. The results were as follows: 1. The ER was present in 35% (n=7) of 20 cases of gastric adenocarcinoma. Carcinomas in male showed ER positivity in 20% while in 40% in female. 2. Histologically, expanding type(n=4) showed 75% positivity of ER while infiltrative type)n=16) showed 13% of positivity. 3. The ER was present in 45%(n=9) of 20 cases of colorectal carcinomas. The male to female ratio of ER positivity was 57%(n=7) to 38%(n=5). 4. All the mucinous carcinomas(n=4) showed ER negativity, but poorly differentiated adenocarcinoma(n=1) and squamous cell carcinoma((n=1) showed ER positivity. 5. Well differentiated adenocarcinoma of the colorectum showed 50%(n=7) positivity of ER. Above results suggest that carcinomas in gastrointestinal tract, which was not considered as estrogen target organ, can express estrogen recetor and the trial of hormone therapy might be highly encouraged.

Prednisolone과 Penicillamine이 사염화탄소 투여로 초래된 백서 간 섬유화에 미치는 영향에 관한 형태학적 연구

김창진,김의한,강상균 순천향대학교 1982 논문집 Vol.5 No.3

The various liver diseases result in fibrosis, which is irreversible and progressively replace normal hepatic parenchyma final]y bringing about hepatic failure. There are two theories for the pathogenesis of hepatic fibrosis. The one is that the fibrosis is resulted from condensation of pre-existing recitulin at the site of collapse of hepatic parenchymal cells, and the other is that the fibroblast is newly formed making collagen fibers and resulting in fibrosis, which is more widely accepted. In the past, the existence of fibroblast in the hepatic lobules was not recognized. But recently, using gold impregnation and ultrastructural studies by electron microscope, lipocytes, which contain many fat droplets in the cytoplasm reducing gold chloride, are recognized in the space of Disse. These cells are considered as precursors of fibroblasts. When they contain excessive vitamin A, which reduces gold chloride, in the cytoplasm, they are activated and transformed into fibroblasts resulting in collagen fiber formation. The present study is undertaken to investigate the role of lipocyte in early hepatic fibrosis and the effect of prednisolone and penicillamine on the early fibrosis induced by carbon tetrachloride and/or vitamin A, which is known to stimulate the lipocytes. By light and electron microscopic examinations following results were obtained. One hundred and twenty female rats, weighing about 200g, were divided into two major groups, control and experimental. The control group was subdivided into salad oil, vitamin A, prednisolone and penicillamine treated groups. The expermental group was subdivided into CCl₄, CCl₄ and vitamin A, CCl₄ and vitamin A with prednisolone, and CCl₄ and vitamin A with penicillamine treated groups. The liver tissue is fixed by perfusion method with mixture of 2% paraformaldehyde and 2% glutaraldehyde. For light microscopic examination the tissue was further fixed in 10% neutral formalin, and stained with hematoxylin-eosin, periodic acid-Schiff(PAS), Masson's trichrome, oil red-O and gold impregnation. For electron microscopic examination, the tissue is further fixed in 3% glutaraldehyde and 1% osmic acid, and stained with combination of uranyl acetate and lead citrate, and periodic acid methenamine silver(PAM), after routine Epon embedding and sectioning. By light and electron microscopic examinations following results were obtained. 1. The control group treated with salad oil only showed inactivated scant lipocytes with little perisinusoidal collagen fibers. 2. Vitamin A treatment induced activation of lipocytes resulting in collagen bundle formation near by or associated with cytoplasmic membrane and collagen fibrils in the cytoplasm. 3. Prednisolone treatment did not induce notable changes of lipocytes. 4. CC1₄ induced hepatic cell necrosis rapidly and accompanied inflammatory cells infiltration. The lipocytes were proliferated and activated at the area of inflammatory reaction and produced new collagen fibers. As the time passed. the collagen fibers decreased in the inflammatory reaction, and the lipocytes returned to resting form. 5. Combined treatment of vitamin A and CCl₄ showed accentuated effects on lipocytes. 6. Prednisolone treatment together with CCl₄, and vitamin A showed inhibition of lipocytic activity and depression of collagen fiber formation. 7. Penicillamine treatment together with CC1₄ and vitamin A showed no different effects. as those treated with CCl₄ and vitamin A. In conclusion, the perisinusoidal fibrosis seen in association with hepatic necrosis was induced by activation of lipocytes, and prednisolone inhibited activation of lipocytes resulting in the depression of early phase of fibrogenesis but penicillamine did not show any effect morphologically.

유방암에서 에스트로젠 수용체와 P-glycoprotein 표출에 대한 면역 조직학적 연구

김창진,강상균,김희석 순천향대학교 1994 논문집 Vol.17 No.4

The P-glycoprotein is a pleotropic membrane transport protein in many normal tissues and tumors. It differs from other membrane transporters in its ability to carry out active transport of apparently unrelated various organic compounds. The relationship of its expression to hormone recptors in breast carcinoma has been studied and its results are controveral. In this study, the relationship between expression of estrogen receptor and P-glycoprotein is studied on 30 primary breast cancers by immunohistochemical method. The results were as follows : 1. Among 30 cases of breast cancer, estrogen receptor was positive in 60%(n=21), while P-glycoprotein was positive in 63%(n=19). 2. Among 21 cases of estrogen receptor positive, P-glycoprotein showed positivity in 71%(n=15) and negativity in 29%(n=6). 3. Among 19 cases of P-glycoprotein positive, moderate to severe lymphatic infiltration was noted in 58%(n=11). 4. P-glycoprotein positive cases(n=19) showed lymphatic invasion in 58%(n=11) and stromal fibrosis of moderate to severe degree in 79%(n=15). The above results suggest that estrogen receptor expression have positive relationship with P-glycoprotein expression and the tumors with P-glycoprotein have some tendency to invade stroma and permeate lymphatics. As the expression of P-glycoprotein in the tumor cells implicates chemotherapeutics resistance and modifies biologic hahaviors of the tumor, forther studies on various tumor should be done.

유방암의 Cytosol Estrogen Receptor 발현율에 대한 면역 조직화학적 연구

김창진,강현욱,강상균 순천향대학교 1990 논문집 Vol.13 No.1

The estrogen receptor status in the breast cancer has been important guideline for the treatment and the prognosis of the patients. This study is carried out by immunohistochemical method using the monoclonal antibody against the cytosol estrogen receptor t investigate the cytosol estrogen receptor(CER) status in various types of breast cancers and to correlate them with ages, histologic grade and other histologic features. The results were as follows; 1. 20 cases(60%) showed CER positivity in 33 cases of breast cancers. 2. Patients with age of below 40 showed CER positivity in 15%, while those with the age above 50 revealed CER positivity in 40%. 3. 16 cases(94%) were above grade Ⅱ in 17 cases of CER positive infiltrating ductal carcinomas. 4. 9 cases(45%) revealed mild or weak lymphocytic infiltration in the stroma in 20 cases of CER positive carcinomas. 5. 13 cases(65%) showed vascular or lymphatic invasion in 20 cases of CER positive carcinomas. 6. 10 cases(50%) showed severe stromal fibrosis around the tumor nests in 20 cases of CER positive carcinomas. Above result suggest that old age, high histologic grade, weak lymphocytic infiltration, frequent vascular or lymphatic invasion and stromal fibrosis have positive relationship with CER positivity.