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Cho, SR,Ock, SA,Yoo, JG,Mohana kumar, B,Choe, SY,Rho, GJ Blackwell Verlag GmbH 2005 Reproduction in domestic animals Vol.40 No.2
<P>Contents</P><P>The present study was designed to examine the effects of cell-cycle synchronization protocols, such as confluent, roscovitine treatment and serum starvation, in bovine foetal fibroblasts on synchronization accuracy at G0/G1, viability, apoptosis, necrosis and ploidy for use as a nuclei donor. The cells in 5–10 passages were randomly allocated into three treated groups. Cells were cultured either in Dulbecco's modified Eagle's medium (DMEM) + 10% foetal bovine serum (FBS) until 90% confluent (group 1, confluent), in DMEM + 10% FBS + 30 <I>μ</I>M roscovitine for 12 h (group 2, roscovitine), or in DMEM + 0.5% FBS for 5 days (group 3, serum starvation). Most of the cells (>80%) in all groups were arrested at the G0/G1 stage. Although the rates did not differ, cells in group 1 showed an increased cell population arrested at the G0/G1 phase. Significantly (p < 0.05) higher rates of apoptosis occurred in group 3 than in group 1 and 2 (10% vs 6% and 6%, respectively). No differences in chromosomal abnormality were observed among groups. However, by increasing the number of cell culture passages up to 15, significantly (p < 0.05) higher chromosomal abnormality was observed than in 5 and 10 passages (39% vs 28% and 23%, respectively) in group 1. The results clearly indicated that bovine foetal fibroblasts could be effectively synchronized at G0/G1 stages by all the three different treatments, confluent, roscovitine and serum starvation. However, cells in confluent showed reduced apoptosis and necrosis when they underwent 5–10 passages, exhibiting increased percentage of cells with stable chromosome diversity. Hence, cells in confluent merit further studies before they could be used as nuclear donors.</P>
조삼현,문형,김두상,정성노,강대진,오명석 대한산부인과학회 1993 Obstetrics & Gynecology Science Vol.36 No.4
저자들은 1989년 12월 한양대학교 의과대학 산부인과학교실에서 57세의 경산부에서 음핵에 발생한 원발성 외음암을 수술전 화학요법과 근치적 외음부 절제술, 방사선조사로 치유한 치험례를 경험하였기에 간단한 문헌고찰과 함께 보고하는 바이다. Primary carcinoma of vulva is a somewhat infrequent entity and accounts for approximately 3~4% of all female genital cancer and less than 1% of all female malignant disease. We experienced a case of primary carcinoma arising from the clitolis in 57 years old multiparous woman. The patients was treated by radical vulvectomy with bilateral groin lymph nodes dissection after neoadjuvant chemotherapy (6 courses) and followed by radiation therapy. Following is a report of this case with a brief review of the lietratures.
김승룡,이재억,조수현,홍은경,황정혜,박병태 대한산부인과학회 1990 Obstetrics & Gynecology Science Vol.33 No.7
자궁의 동정맥기형은 1926년 Dubreuil등이 처음 보고한 이래 지금까지 30예 밖에 보고되지 않은 매우 드문질활으로 국내에서는 아직 보고된 바 없다. 이 질환은 주로 비정상 자궁출혈을 초래하며 임심시에는 다량의 산후출혈을 유발할 수 있으며 이 경우 소파술은 출혈을 더욱 악화시킨다. 동맥조형술, 자궁경 또는 초음파검사로 진단이 되나 대부분이 자궁적출 후 조직병리검사로 확인된다. 저자들은 최근 제왕절게 분만한 2주일후부터 심한 자궁출혈이 있어 내원한 24세 초산부에서 전자궁 적출술을 시행하여 자궁혈관기형이 확인된 1예를 경험하였기에 문헌고찰과 함께 보고하는 바이다. Arteriovenous malformation of the uterus is extremely rare and only 30 cases have been reported in literature. A patient presented with massive postpartum bleeding which was aggrevated after curettage and was treated with total abdominal hysterectomy.
김승룡,조삼현,황윤영,유중배,문영진,황정혜,고은선,이원준,이재웅 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.10
저자들은 기존의 심장 질환이 없었던 임신 후기의 산모에서, 배양 음성의 아급성 세균성 심내막염을 경험하였기에 보고하는 바이다. Infective endocarditis is uncommon complication in pregnant women but it is virtually always fatal if untreated. Therefore, early diagnosis, prompt medical or surgical intervention are essential for favorable maternal and fetal outcome. Recently, we experienced a case of infective endocarditis in a 28 year-old women in 35 weeks gestation who presented prolonged fever, malaise, pain and motor weakness of lower extremites, and petechia. We present this case with brief literature review.
자궁경부암 환자에서 말초혈액 임파구를 이용한 림프킨활성살세포의 항암 효과 및 그 표현형에 대한 연구
김승룡,김경태,조삼현,황윤영,이재억,문형,김정목,조양자 대한부인종양 콜포스코피학회 1992 Journal of Gynecologic Oncology Vol.2 No.1
In adoptive immunotherapy of cancer using lymphokine-activated killer(LAK) cells induced from patients own peripheral blood, the efficient cytotoxicity of LAK cells is a principal requirement. Peripheral blood lyrnphocytes were obtained from 15 patients with cervical cancer and 13 normal women as a control, and LAK cells were induced with r-interleukin-2(rlL-2). Cytotoxic activity of cultured cells were measured by a 4h-51 Cr release assay using NK(natural killer)-sensitive K-562 cell line and NK-resistant RAJI target cells, and their surface phenotypes were determined by an imrnunofluorescence significantly increased compared with those activity without rIL-2. Despite the ratio between effector and target cells, the cytotoxic potential of NK and LAK cells of normal women were significantly higher than those of cervical cancer patients. (FO) However, LAK ce11 activity was relatively low but increased significantly in patients with cervical cancer. NK cell and LAK cell cytotoxicity in the group of tumor mass, 4 cm were significantly decreased compared with those in the group of tumor mass 4 cm. (Effector: target ratio = 50:1, NK: P=0.038, LAK : P = 0. 033) Surface phenotypes of peripheral lymphocytes and LAK cells were examined with indirect immunofluorescence method, The CD 3 subpopulations which are known to be major components af LAK cells were not diminished. After LAK cell induction with IL-2, the percentages of CD l6(Natural killer cell) populations were significantly increased in normal(11.2% vs 48.3%) and cervical cancer patients(11.8% vs 47.8 %). These data suggest that 1) the decline of NK and LAK cell act.ivity in cervical cancer patients, 2) the decreasing cytotoxicity with the increasing tumor mass, 3) the percentage of natural killer cell subpopulation dramatically increased with surface phenotype of LAK cells, 4) LAK cells may be applied for passive adoptive immunotherapy.
자궁경부암 환자에 있어 telomerase 활성도의 임상측명
김승룡,김경태,조삼현,황윤영,문형,유중배,김기성,라명재 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.4
A current hypothesis gaining prominence proposes that activation of the telomerase is necessarey for cells to become immortal, or be capable of proliferating indefinitely. The theory suggersts that that almost all cancer cells must attain immortality for progression to malingnant state and, hence, require activation of telomerase. To assess the role of telomerase in the development of mailignant transformation of the uterine cervical carcinoma, telomerase activeiy was measured by using a recently developed sensitive RPR-based telomerase assay(telomeric repeat amplification protocol; TRAP)in benign uterine condition and invasive uterine cervical carcinoma tissues revealed to be telomerase positive (14/16, 88%) which represents a characteristic 6 bp ladder pattern, while none of the 4 cervical tissues with the nonspecific pathologic findings or just chronic cervictis which were obtained by simple bysterenctomy under the diagnoses of benign myoma or uterine prolapse showed any telomerase actiovity. These findings suggest that this enzyme activity may play a key role in the establishment and progression of the uterine cervical carcinoma. This activity of telomerase may influence from the initial stage of tumorigenesis of uterine cervical carcinoma because poitive findings were noticed from the early stage (stage (i 1a1) to the advanced stage. The results of neoadjuvant chemotherpy which were revealed in final histopathologic specimens following radical hysterectomuy, pelvic and raraaortic lymph nodes dissection (suggestion by Rosen et al,) were one grade IV wiht telomerase poistive, 2 grade III with all positivie, 5grade II with 3 positive 2 negative. The two cases of pelvic lymph node metastasis belong to the group of grade II and their activeities of telomerase were 1 positive and 1negative. The situation of apoptosis induced by antineoplastic chemotherapy has not been related to that of telomerase activeity. Furthemore, the late recurrences has been noticed over 5years follow-up following neoadjuvant chemotherapy and radical surgery for the management of the high risk group of patients (not shown in this study) This measn that the nowday`s regimen for induction tumoricidals may have been operating as dual effects (partial restoring and redamaging) to the cell cycle chekpoint. Therefore if telomerase inhibitor will be applied combined whit the induction chemotherapy, unexpected benefits mignt be obtained for the patient with the advanced uterine cervical carcinoma.