자궁경부암 병기판정 기초검사시 새로운 보조방법으로서 고주파 질식 초음파의 가치

유희석,이은주,장기홍,권혁찬,양정인,김행수,오기석 아주대학교 의과학연구소 1996 아주의학 Vol.1 No.1

To investigate the efficacy of endoluminal ultrasound system (EUS) as a surrogate for high frequency transvaginal ultrasonography and as an optional baseline study in determining parameirial and stromal invasion of early stage cervical cancer, 52 women suspected of cervical cancer underwent EUS. A 12 MHz endoscopic probe was employed to radially scan the cervix for possible lesions suspected to be invasive cancer during a period of 6 months from Feb. 1 to July 1, 1995. Patients also underwent magnetic resonance imaging (MRI) and/or computerized tomography (CT) as a routine mode of baseline study, and were clinically staged by 3 independent physicians specializing in Obstetrics and Gynecology at the Department of Obstetrics and Gynecology, School of Medicine, Ajou University, Suwon, Korea. Thirty one patients subsequently received surgery appropriate for the stage of the disease and the final pathology findings were compared with the results of clinical staging, EUS, MRI/CT by regression analysis. The results showed that there was statistically significant correlation between MRI/CT and pathology (r=0.660, p<0.02), between EUS and pathology (r=0.803, p<0.01), and between clinical staging and pathology (r=0.825, p<0.01). It is concluded that there was significant statistical correlation t>etween EUS, MRI/CT, clinical staging and pathology, but the question remained as to the statistical superiority of EUS over MRI/CT with regard to parametrial invasion and stromal invasion depth assessment. Finally, EUS is useful as an alternative optional diagnostic tool in the baseline study of cervical cancer.

돈사폐수를 이용한 Polyhydroxyalkanoates의 생산

조경숙,류희욱,박창호 이화여자대학교 생명과학연구소 1996 생명과학연구논문집 Vol.7 No.-

돈사페수를 발효기질로 이용한 생분해성 고분자, polyhydroxyalkanoates(PHA)의 생산이 가능하였다. 균주로는 질소 혹은 인 등의 비탄소 영양원의 제한 없이도 PHA의 생산이 가능한 Azotobacter vinelandii UWD를 이용하였고 돈사폐수배지에서의 PHA 생산특성을 glucose 합성배지에서의 특성과 비교하였다. A. vinelandii uwd균주는 탄소원으로 glucose와 alkanoates가 함유된 배지에서 alkanoates를 우선적으로 소비하여 주로 생장을 하고, glucose 소비단계에서는 생장보다는 PHA를 생산하는 diauxic growth를 하였다. 돈사폐수 원액을 배지로 사용할 경우 UWD 균주의 건조균체량과 PHA함량이 낮았는데(0.91 g/L,37 wt%). 이는 돈사폐수 중에 함유되어 있는 acetate, propionate, butyrate 및 valerate 등과 같은 높은 농도의 alkanoates가 세포생장을 저해하기 때문이었다. 증류수로 50% 희석한 배지(총 생산 균체 건중량 2.02g/L)를 사용하는 것이 바람직한 것으로 밝혀졌다. 50% 희석 돈사폐수 배지에 glucose를 3% 첨가하면 건조균체량은 9.40g/L로 현저하게 증가하였으며, 균체내 PHA 함량은 58.3%, PHA 생산속도는 0.11g/(L·h)이었다. Glucose 합성배지에서 합성된 PHA는 poly-β-hydroxybutyrate(PHB)의 형태이었고, 돈사폐수 배지로부터는 copolymer인 poly-β-hydroxybutyrate-co-β-hydroxyvalerate, P(HB-co-HV)를 2-8mol% 합성할 수 있었다. 돈사폐수배지를 이용하여 PHA를 생산하는 동안 배지의 염도를 대략 50% 정도 제거가 가능하였다. Biodegradable polymer, polyhydroxyalkanoates(PHA), could be produced using swine wastewater (SW)as a fermentation substance. Azotobacter vinelandii UWD was able to produce PHA without limiting non-carbon nutrients such as nitrogen and phosphorous. Production characteristics of strain UWD in SW medium were compared with those in glucose sythetic medium In the synthetic medium containing glucose and alkanoates as carbon sources. A vinelandii UWD grew diauxically, first by using alkanoates and then by using glucose to produce biomass and PHA. Using raw SW medium, cell mass production was small(0.91 g/L)and PHA content was 37 wt% because cell growth was inhibited by alkanoates such as acetate, propionate, butyrate, and valerate contained in SW. Use of two-fold diluted SW as fermentation medium was desirable for PHA production. When 3% of glucose was added to the two-fold diluted SW, dry cell mass increased to 9.40 g/L, and the PHA content and PHA production rate were 58.3 wt% and 0.11g/(L·h), respectively. When grown in SW media A. vinelandii UWD formed PHA copolymers containing β-hydroxybutyrate and β-hydroxyvalerate(HV), and HV content in the copolymer was 2-8mol% whereas homopolymer of poly-β-hydroxybutyrate was produced in the glucose synthetic medium. Approximately 50% of salinity in the medium was reduced after PHA production using SW medium.

키토산과 견사 단백질 복합체의 특성

김희숙,원용돈,류병호 한국식품영양학회 1996 韓國食品營養學會誌 Vol.9 No.4

기능성 다이어트 식품을 개발을 목적으로, 키토산과 견 피브로인의 복합체를 만들고 성질을 조사하였다. 키토산은 대동새우로부터 추출하였고, 견사 피브로인 섬유는 누에로부터 추출하였다. 견사의 특성을 알아보기 위하여 용해도를 조사한 결과, 용해도는 8M LiBr이상의 농도와 40℃ 이상에서 가장 높았다. 견사 피브로인의 아미노산 조성은 천연견사 및 용해시킨 피브로인과 같았다. 키토산, 견사 피브로인 복합체의 아미노산 조성은 glycine, alanine, serine, tyrosine, threonine 및 glutamic acid 순서를 많았다. 또한 IR 스펙트럼 결과, 키토산·견사 피브로인 복합체는 배합비율에 따른 스텍트럼상의 차이는 거의 없었다. In order to develop the functional diet food, characteristics of chitosan and silk fibroin composite was designed. Chitosan extracted from a prawn and silk fibroin was prepared from silkworm. The silk fibroin was dissolved rapidly in the 8M LiBr at a temperatrue of more than 40℃. Amino acid composition of fibroin composite revealed the same pattern that of native silk fibroin and regenerated silk fibroin. Predominant amino acid of chitosan-fibroin composite contained glycine, alanine, serine, tyrosine, threonine, and glutamic acid in order. According to the basis on the infrared spectrum, chitosan-silk fibroin composite is not distinguished differents composite ratio of chitosan and silk fibroin.

돈사폐수를 이용한 Polyhydroxyalkanoates의 생산

조경숙,박창호,류희욱 이화여자대학교 환경문제연구소 1997 이화환경연구 Vol.1 No.-

돈사폐수를 발효기질로 이용한 생분해성 고분자, polyhydroxyalkanoates(PHA) 의 생산이 가능하였다. 균주로는 질소 혹은 인 등의 비탄소 영양원의 제한 없이도 PHA 의 생산이 가능한 Azotobacter vinelandii UWD 를 이용하였고 돈사폐수배지에서의 PHA 생산특성을 glucose 합성배지에서의 특성과 비교하였다. A. vinelandii UWD 균주는 탄소원으로 glucose와 alkanoates 가 함유된 배지에서 alkanoates를 우선적으로 소비하여 주로 생장을 하고, glucose소비단계에서는 생장보다는 PHA를 생산하는 diauxic growth 를 하였다. 돈사폐수 원액을 배지로 사용할 경우 UWD 균주의 건조균체량과 PHA 함량이 낮았는데(0.91g/L, 37wt%), 이는 돈사폐수 중에 함유되어 있는 acetate, propionate, butyrate 및 valerate등과 같은 높은 농도의 alkanoates가 세포생장을 저해하기 때문이었다. 증류수로 50% 희석한 배지(총 생산 균체 건중량 2.02g/L)를 사용하는 것이 바람직한 것으로 밝혀졌다. 50% 희석돈사폐수 배지에 glucose를 3% 첨가하면 건조균체량은 9.40g/L로 현저하게 증가하였으며, 균체내 PHA 함량은 58.3%, PHA 생산속도는 0.11g/(L·h)이었다. Glucose 합성배지에서 합성된 PHA는 poly-β-hydroxybutyrate(PHB)의 형태이었고, 돈사폐수 배지로부터는 copolymer인 poly-β-hydroxybutyrate-co-β-hydroxyvalerate, P(HB-co-HV)를 2-8mol%합성할 수 있었다. 돈사폐수배지를 이용하여 PHA를 생산하는 동안 배지의 염도를 대략 50% 정도 제거가 가능하였다. Biodegradable polymer, polyhydroxyalkanoates(PHA), could be produced using swine wastewater (SW) as a fermentation substance. Azotobacter vinelandii UWD was able to produce PHA without limiting non-carbon nutrients such as nitrogen and phosphorous. Production characteristics of strain UWD in SW medium were compared with those in glucose synthetic medium. In the synthetic medium containing glucose and alkanoates as carbon sources. A. vinelandii UWD grew diauxically, first by using alkanoates and then by using glucose to produce biomass PHA. Using raw SW medium, cell mass production was small (0.91g/ L) and PHA content was 37wt% because cell growth was inhibited by alkanoates such as acetate, propionate, butyrate, and valerate contained in SW. Use of two-fold diluted SW as fermentation medium was desirable for PHA production. When 3% of glucose was added to the two-fold diluted SW, dry cell mass increased to 9.40g/L, and the PHA content and PHA production rate were 58.3wt% and 0.11g(L·h), respectively, When grown in SW media A. vinelandii UWD formed PHA copolymers containing β-hydroxybutyrate and β-hydroxyvalerate (HV), and HV content in the copolymer was 2-8mol% whereas homopolymer of poly-β-hydroxybutyrate was produced in the glucose synthetic medium. Approximately, 50% of salinity in the medium was reduced after PHA production using SW medium.

폐탄광 배수에 의한 주변 화천 오염 실태 조사

조경숙,장용근,류희욱 崇實大學校 生産技術硏究所 1996 論文集 Vol.26 No.1

The survey was carried out to investigated the contamination of streams by the acid mine drainage originates from abandoned coal mines and coal refuse piles. The physico-chemical characteristics such as pH, sulfate and elements concentrations of waters and sediments in streams were analyzed, and the microbial activities in the sediments were evaluated by measuring dehydrogenase activities. In sites contaminated by the acid mine dranage, the pH of waters adn sediments declined to acid from neutral due to the accumulation of sulfate. Dehyfrogenase activities ranged from 12 to 170 ㎍-TPF·g-dry soil-¹·24h-¹in the contaminated sites, whereas teh uncontaminated sites had activities of 1,176-4,259 ㎍-TPF·g-dry soil-¹·24h-¹. Dehydrogenase activities were significantly affected by low pH of sediments, indicating that high strength of sulfate inhibited microbial activities. The concentrations of heavy matals such as Pb and Fe in contaminated sediment (37-46 ppm Pb; 46,000 ppm Fe) were much higher than those in the uncontamnated sediments. concentration of Al in the contaminated water acidfied by the abandoned coal drainage was in range of 11 to 42 ppm. compared with those in the uncontaminated sediments, the concentrations of Mn, Mg and Ca in contaminated sediments were low because of the leaching of them from soil to water by acidfied stream water.

ASTM F813-07 의료용디바이스를 위한 생체재료의 직접촉 세포배양측정에 대한 표준지침서

김순희 ( Soon Hee Kim ),백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2,),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4

We provided standard practice for direct contact cell culture evaluation of materials for medical devices in this report. This practice introduced a reference method of direct contact cell culture testing. This method of direct contact cell culture may be used in the construction of medical materials and devices for test the cytotoxic potential of materials. Researcher for medical devices could use this practice to compare other cytotoxicity tests or directly to evaluate materials. Cell or materials could be sometimes modified depending on use in offered extend of this practice.

젊은 성인 남자의 흡연과 혈청 지질과의 관련성

조선,류소연,박종,강명근,김은숙,김형철,박광희,윤혜은,노희송 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Objectives: This study was performed to find the association of cigarette smoking and blood lipid profiles in healthy young adults. Methods: The data was collected by a self-administered questionnaire and health examination in 489 professional soldiers from 6 to 12 May, 2004. The status of cigarette smoking was classified by non-smoking, ex-smoked, ≤4.9, 5-9.9, and ≥10 pack-years. The statistical analysis were used by chi-square test, analysis of variance and analysis of covariates. Results: In status of subjects cigarette smoking, current smoking, ex-smoked, and non-smoking were 71.8%, 11.2%, and 17.0%, respectively, In distribution by amount of smoking, non-smoking, ex-smoked, ≤4.9 pack-years, 5-9.9 pack-years, and ≥10 pack-years were 17.0%, 11.2%, 37.4%, 21.3%, and 13.1%, respectively. At other confounding variables were controlled, total cholesterol and LDL-cholesterol were increased significantly when amount of smoking was increased. However, triglyceride and HDL-cholesterol did not showed statistically significant differences, Conclusion: Smoking has relevant to total cholesterol and LDL-cholesterol and it is need to perform the further study for finding how blood lipid profiles are affected its level by cigarette smoking and to find an influence of cigarette smoking on cardiovascular disease.

경연대회를 통한 초·중고생의 창의력 현황에 관한 연구

육근철,이희복,류해일,김현섭,김희수,박달원,심규철,박종석,신영숙,박상태,변두원,김태균 공주대학교 과학교육연구소 2002 과학교육연구 Vol.33 No.-

21세기의 정보화 산업사회와 지식기반 사회에서 우리의 아이들이 자신의 목소리로 세계 속에서 살아가게 하기 위해서는 다른 사람과 다른 아이디어를 낼 수 있도록 교육의 틀을 바꾸지 않으면 안된다. 다라서 21세기의 미래 사회를 살아갈 학생들을 가르치는 교육의 현장 역시 창의적인 인간을 길러낼 수 있도록 변해야 한다. 즉, 미래 사회를 대비하기 위해서는 각 개인의 개성을 존중하는 분위기에서 창의적인 능력을 지닌 인간을 양성해야 한다는 것을 의미한다. 따라서, 학교 안에서의 교육뿐만이 아니라 학교 밖에서도 다양한 학습 경험을 통해서 교육 과정이 추구하는 인간상을 구현할 수 있도록 활동할 수 있는 기회가 학생들에게 제공되어야 한다. 창의력을 발휘하여 문제를 해결하는 능력을 기르기 위해서는 개별적이 아닌 여러 명의 동료가 주어진 과제를 해결할 수 있는 기회를 갖고 짧은 시간이 아닌 장시간 동안 고민하고 그 해결책을 찾는 활동을 가질 기회가 필요하다. In order to educate our students with their special talents for the informational and knowledge based age expected in the 21st century, we must improve our educational programs such that students are able to create their own fresh ideas. Hence the present educational environment should be changed such that they are willing to respect others' characters and personality with their own original ideas. Therefore, we must make them have the opportunity that they can acquire many experiences through the school education and the social education program, so that they may grow up to the person with the humanity and creativity. In this study we have suggested new educational program where students engage themselves in long term research projects with collaboration of the team and creativity of individuals.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.

ASTM F2027 조직공학적 의료용제품(TEMPs)을 위한 기질의 특성결정 및 테스트를 위한 표준 지침서

백미옥 ( Mi Ock Baek ),소정원 ( Jung Won So ),김순희 ( Soon Hee Kim ),노혜원 ( Hye Won Roh ),이나리 ( Na Ri Lee ),김문석 ( Moon Suk Kim ),유규하 ( Gyu Ha Ryu2 ),조양하 ( Yang Ha Cho ),이승진 ( Seung Jin Lee ),강길선 ( Gilson Khang 한국조직공학·재생의학회 2007 조직공학과 재생의학 Vol.4 No.4

Tissue engineering medical products are those protocols and products developed for use in the human body as biological substitutes to restore, maintain, or improve tissue function. The purpose of this standard is to locate relevant existing guideline and test methods and to provide guidance for interim use of materials for which a standard does not exist. The standard may be use as guideline in tissue engineered research.