Bacillus subtilis 接種이 土壤微生物相에 미치는 影響(Ⅰ) : 볏짚 및 크로버 添加區에서의 微生物相의 變動 Changes in Microflora by Application of Straw and Clover

孔惠淑,柳恩珠,梁昌述 建國大學校 附設 農業資源開發硏究所 1993 農資源開發論集 Vol.18 No.-

토양으로부터 분리한 B. subtilis를 有機物이 施用된 토양에 還元 接種하여 有機物 種類에 따른 B. subtilis의 토양중에서 適應·定着性 및 B.subtilis가 다른 土壤微生物相에 미치는 영향을 經時的으로 조사하였다. 또한 有機物이 시용된 토양중에서 B,subtilis와 植物 病原菌인 F. oxysporum을 동시에 接種하여 상호간의 拮抗的 關係를 검토한 결과는 다음과 같다. 1) B. subtilis菌數는 對照區에서보다 B. subtilis 接種區에서 增加를 보였고 단지 볏짚 및 크로버만을 사용한 區에서보다 볏짚 및 크로버 施用區에 B. subtilis를 접종한 區가 약 2배 정도 B. subtilis菌數의 증가를 보였다. 특히 B.subtilis를 有機物 施用區에 접종했을 때 볏짚 시용구가 크로버 시용구에서보다 B. subtilis菌數가 약 3배 정도 현저하게 증가하였다. 2) 細菌과 絲狀菌數는 대조구보다 B.subtilis 接種區에서 각각 23% 및 20%씩 감소하였으므로 또한 크로버 시용구에서보다 크로버+B. subtilis 접종구에서도 각각 33% 및 16%씩 감소하였고, 볏짚 시용구에서는 볏짚+B. subtilis 접종구에서 각각 29% 및 32%씩 감소경향을 나타내었다. 3) 胞子形成菌數는 대조구보다 B. subtilis 접종구에서 약 10배 정도 증가하였으며 단지 有機物만을 시용한 區보다도 크로버+B. subtilis 접종구와 +B. subtilis 접종구에서 각각 2배, 4배 정도의 증가경향을 보였다. 또한 gram(-)細菌도 胞子形成菌數와 비슷한 경향을 나타내었고, B. subtilis接種이 그람 음성 세균수의 변동에는 영향을 미치지 않았다. 4) F. oxysporum의 菌數는 크로버 시용구에서보다 크로버+F. oxysporum 접종구에서 25배 정도 증가하였으나 크로버+B. subtilis 접종구에서는 크로버+F. oxysporum 접종구보다 약 5배 정도의 감소경향을 나타내었다. 또한 F. oxysporum菌數는 볏짚+F. oxysporum區에서 볏짚 單獨施用區보다 3배 정도 증가하였으나 볏짚+B. subtilis+F. oxysporum접종구는 볏짚+F. oxysporum접종구보다 F. oxysporum의 數가 약 17배 정도 감소경향을 나타내었다. 더욱이 F. oxysporum菌數는 크로버+B. subtilis+F. oxysporum접종구보다 볏짚+B. subtilis+F. oxysporum접종구에서 약 40배 정도의 감소경향을 나타내었다. 5) 크로버 시용구의 토양 pH는 약 ph 9에 이르러 토양 ph가 alkali화되었으며 볏짚시용구의 토양 ph는 큰 변화 없이 약산성을 유지하였다. This study has been conducted to investigate the adaptation of Bacillus subtilis to the soil and the antagonistic interaction between B. subtilis and pathogen Fusarium oxysporum as well as the periodic change of soil microflora when B. subtilis was inoculated to the soil treated with organic matters. Some results obtained from this study are as follows : 1) The number of B. subtilis was more increased about 6 times in the plot inoculated with B.subtilis than in control plot. It was relatively double in the organic matter+ B. subtilis plot than in the only organic matter plot. B. subtilis was particuraly increased about 3 times in the straw +B. subtilis plot, compared with the clover +B. subtilis plot. 2) The number of total bacteria and fungi was decreased by 23% and 20% in the plot inoculated with B.subtilis respectively than in control plot. It was not only decreased by 33% and 16% in the clover+B. subtilis plot respectively than in the only clover plot but it was also decreased by 29% and 32% in the straw+B. subtilis plot respectively than in the only straw plot. 3) The number of spore-forming bacteria was increased about 10 times in the plot inoculated with B.subtilis than in the control plot. It was also increased 2 times and 4 times in the clover+B.subtilis plot and in the straw+B. subtilis plot respectively than in each organic matter plot. The number of gram-negative bacteria and the spore-forming bacteria were slightly increased in all the plot inoculated with B. subtilis. 4) The number of F. oxysporum was increased about 25 times in the clover+ F. oxysporum plot than in the only clover plot. But it was decreased about 5 times in the clover+B.subtilis+ F. oxysporum plot than in the clover +F. oxysporum plot. Furthermore, F. oxysporum was remarkably decreased about 40times in the straw+B. subtilis+ F. oxysporum plot than in the clover+b. subtilis+ F. oxysporum plot. It occured that B. subtilis had inhibited the growth of pathogen F. oxysporum when B. subtilis was inoculation to the soil treated with straw. 5) Soil pH was changed to pH 9 in the plot treated with clover to become the alkalification of experimental soil. Meanwhile it was not changed in the plot treated with straw.

젊은 성인 남자의 흡연과 혈청 지질과의 관련성

조선,류소연,박종,강명근,김은숙,김형철,박광희,윤혜은,노희송 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Objectives: This study was performed to find the association of cigarette smoking and blood lipid profiles in healthy young adults. Methods: The data was collected by a self-administered questionnaire and health examination in 489 professional soldiers from 6 to 12 May, 2004. The status of cigarette smoking was classified by non-smoking, ex-smoked, ≤4.9, 5-9.9, and ≥10 pack-years. The statistical analysis were used by chi-square test, analysis of variance and analysis of covariates. Results: In status of subjects cigarette smoking, current smoking, ex-smoked, and non-smoking were 71.8%, 11.2%, and 17.0%, respectively, In distribution by amount of smoking, non-smoking, ex-smoked, ≤4.9 pack-years, 5-9.9 pack-years, and ≥10 pack-years were 17.0%, 11.2%, 37.4%, 21.3%, and 13.1%, respectively. At other confounding variables were controlled, total cholesterol and LDL-cholesterol were increased significantly when amount of smoking was increased. However, triglyceride and HDL-cholesterol did not showed statistically significant differences, Conclusion: Smoking has relevant to total cholesterol and LDL-cholesterol and it is need to perform the further study for finding how blood lipid profiles are affected its level by cigarette smoking and to find an influence of cigarette smoking on cardiovascular disease.

응급 처리한 Alcaligenes eutrophus 균체로부터 poly(3-hydroxybutyrate)의 회수

장용근,홍은화,류희욱,조경숙 이화여자대학교 환경문제연구소 1998 이화환경연구 Vol.2 No.-

생분해성 고분자인 poly(3-hydroxybutyrate, PHB)를 생산하는 Alcaligenes eutrophus의 배양액에 응집제를 첨가하여 회수한 균체로부터 용매추출법에 의한 PHB 회수와 순도에 미치는 응집제의 영향을 조사하였다. 응집제는 Al계 3종류와 Fe계 2종류를 사용하였다. 균체로부터 PHB는 30%로 희석한 hypochlorite용액과 chloloform혼합용액을 이용하여 추출 ·회수하였다. Al계 응집제들의 경우, 응집제의 첨가량이 1,000mg-Al/L이하의 농도에서는 PHB회수율이 응집제를 첨가하지 않은 경우의 회수율(82%)과 거의 비슷하였고, 1000mg-Al/L이상의 농도에서는 응집제 첨가량이 증가함에 따라 PHB 회수율이 감소하였다. PHB 추출에 사용한 hypochlorite용액의 농도를 50%로 증가시키면, 응집제를 2000mg-Al/L까지 높은 농도로 첨가하여도 90% 이상의 PHB회수율을 얻을 수 있었다. 회수된 PHB의 순도는 응집제의 종류와 용량에 관계없이 98%이상으로 매우 높았다. Fe계 응집제의 경우, 응집제의 용량은 PHB의 회수율에 거의 영향을 미치지 않았으나, 회수된 PHB중의 잔류 Fe의 농도가 높았고, 잔류 Fe에 의해 정제한 PHB가 엷은 적색으로 착색되었다. 세포의 회수와 PHB의 정제공정에 적합한 응집제로는 응집효율, 잔류Al과 Fe및 정제PHB의 색상 등을 고려할때 Fe계 보다는 Al계 응집제가 적합하였다. The effects of the pretreatment with coagulants on the recovery efficiency of poly(3-hydroxybutyrate, PHB) synthesized in Alcaligenes eutrophus were investigated. Al-base or Fe-base coagulants, and the dispersion method of 30% hypochlorite solution and chloroform were used as coagulants and PHB recovery method, respectively. The recovery efficiency of PHB from the cells harvested with Al-base coagulants at the range from 0 to 1000mg-Al/L, was similar to that from cells harvested without the coagulants. At these conditions, the concentrations of residual aluminium in the purified PHB were below 250mg-Al/kg-PHB, indicating the effect of residual aluminum on the characteristics of the purified PHB can be insignificant. When the dosage of coagulants was over 1000mg-Al/L, the PHB recovery remarkably decreased with increasing the coagulant dosage. However, the PHB recovery could be enhanced by the use of 50% efficiency was not found by using Fe-base coagulants, the purified PHB was stained pale red due to residual iron, These results suggest that the use of Al-base coagulants did not exert bad influence on neither PHB recovery efficiency and PHB purity.

실내 기후동에서 개인형 국소냉방 장치의 기류 및 온도 분포해석

엄태인,장은숙,경남호,신기식,문승현,전영남,류창국 한밭대학교 생산기반기술연구소 2002 생산기반기술연구소 논문집 Vol.2 No.1

Numerical simulation is performed by the commercial code FLUENT for individual air condition system to calculate the velocity and temperature profiles around worker in climate room. The main calculation region is the space of ㄱ shape between wall and worker in the individual air condition system. The fresh air is emitted from 3 inlet attached on the right, left and center wall. Because the comfort of worker is closely related with the velocity and temperature of wind, these data are key role for design of the individual air condition system. This work is previous study for evaluation of thermal comfort of workers in room without much experiments according to metabolism and heat emission of human body. Numerical simulation results are as followings, 1. There is an possibility of the thermal comfort of worker is gotten worse due to the presence of dramatic change of wind velocity around worker. 2. Temperature gap exists in worker's body between top and bottom according to fresh air inlet velocity and position which is right, left and center of wall. 3. Calculation results of the skin temperature of worker are key data for evaluation of thermal comfort with experimental data in climate room. 4. Optimum condition of temperature, direction and velocity of fresh air will be obtain by numerical simulation for energy effective individual air condition system.

거대 난소 종양의 염전 1예

김미영,이해혁,유애리,박정희,이임순,정수호,고은석,레앙소팥,배동한,김태희,이권해 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

..

내분비계 교란물질의 검출계를 이용한 γ-HCH의 미생물에 의한 중간대사산물에 대한 내분비계교란 활성의 평가

이행석,박주석,조은민,문명숙,太田明德,류재천 대한상하수도학회 2003 상하수도학회지 Vol.17 No.1

To develop an efficient degradation system for Endocrine disruptors (EDs), it is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. We previously constructed a co-expression system of GAL4 DNA binding domain (DBD)-human estrogen receptorβ ligand binding domain (hERβ LBD) and Gal4p transcriptional activation domain (TAD)-co-activator SRC1 in Saccharomyces cerevisiae with a chromosome-integrated lacZ reporter gene under the control of CYC1 promoter and GAL4 binding site (Upstream Activating Sequence, UAS). Expression of this reporter gene was dependent on the presence of estrogen or endocrine disruptors in the culture medium. Furthermore, the extent of transcriptional activation by those chemicals correlated with their estrogenic activities measured by other assay systems, indicating that this assay system is efficient and reliable for measuring estrogenic activity. We applied this assay system to measure estrogenic activity of microbial degradation products of γ-hexachlorocyclohexane (γ-HCH) by Sphingomonas paucimobilis. Among the γ-HCH metabolites, 2,5-dichlorohydroquinone (2,5-DCHQ) and chlorohydroquinone (CHQ) had similar estrogenic activities to the original chemical, but hydroquinone (HQ), a metabolite at later stage, had no activity at the concentration of 10^-4M, showing the necessity of evaluation of intermediate metabolites in microbial degradation systems.

입원 중인 베트남 참전 군인의 삶의 만족도와 관련된 요인

김애리,박종,김율,노희송,박광희,김은숙,김형철,홍강식,류소연,강명근,김기순 朝鮮大學校 附設 醫學硏究所 2007 The Medical Journal of Chosun University Vol.32 No.2

Background and Objectives: The aim of the study was to identify the factors associated with life satisfaction of hospitalized Vietnam veterans. Material and Methods: The data were collected from 200 hospitalized Vietnam veterans of 5 veterans hospitals in Korea from September 13 to 23, 2004. Information of general characteristics, health behavior, disease history, family function, depression and life satisfaction were collected by structured questionnaire. Results: The mean of life satisfaction was 21.48(±6.03), and the mean of depression was 53.10(±9.64). The prevalence of depression was mild depression 24.0%, moderate depression 43.5% and severe depression 25.0%. Depression was associated with life satisfaction and depression was higher, life satisfaction was lower. Family function and age were positively associated with life satisfaction, Conclusion: Life satisfaction was affected by depression and family function in hospitalized Vietnam veterans. Therefore, it was thought that psychosocial approaches such as improve depression and family function in care of hospitalized Vietnam veterans is required.

노년층 제2형 당뇨병환자의 가족 지지 정도와 혈당 조절과의 관계

김은숙 ( Eun Suk Kim ),이성진 ( Seong Jin Lee ),류옥현 ( Ohk Hyun Ryu ),이지연 ( Jee Youn Lee ),윤현숙 ( Hyun Suk Yun ),최문기 ( Moon Gi Choi ) 대한당뇨병학회 2007 Diabetes and Metabolism Journal Vol.31 No.5

한국·중국 공기업의 신뢰가 조직효과성에 미치는 영향

류은영(Eun Young Ryu),류병곤(Byung Gon Ryu),류은숙(Eun Suk, Ryu) 한국인사행정학회 2017 한국인사행정학회보 Vol.16 No.3

최근 중국경제 및 중국조직에 대한 관심이 고조되고 있다. 하지만 중국의 조직과 관련한 실증연구는 여전히 제약적이다. 이에 본 연구는 신뢰, 조직몰입, 조직효과성 간의 인과관계를 분석함에 있어 한국과 중국에 소재한 공기업을 대상으로 하여 표본을 수집하고 실증분석을 실시하였다는 점에서 의의가 있다. 분석방법은 다중회귀분석과 매개회귀분석을 사용하였으며, 분석결과는 다음과 같다. 첫째, 한국과 중국의 신뢰가 조직몰입에 미치는 영향은 차이가 있는 것으로 분석되었다. 둘째, 한국과 중 국의 신뢰가 조직효과성에 미치는 영향은 차이가 있는 것으로 분석되었다. 한국의 경우 신뢰유형 중 동료 신뢰, 상사신뢰 순으로 조직효과성(조직시민행동, 혁신행동)에 영향을 미치는 것으로 분석되었다. 중국의 경우 상사신뢰는 조직효과성(조직시민행동, 혁신행동)에 아무런 영향을 미치지 않는 것으로 분석되었고, 동료신뢰만이 조직효과성(조직시민행동, 혁신행동)에 영향을 미치는 것으로 분석되었다. 셋째, 한국과 중국 모두 신뢰가 조직효과성에 미치는 영향에서 조직몰입의 매개효과를 확인하였다. 중국 의 경우 조직몰입은 동료신뢰가 혁신행동에 미치는 영향에서만 부분매개효과가 있는 것으로 분석되었다. 한국은 상사신뢰, 동료신뢰가 조직효과성(조직시민행동, 혁신행동)간의 관계 모두에서 부분매개효과가 있는 것으로 분석되었다. 한국과 중국의 국가별 신뢰와 효과성에 대한 차이를 통해 경영관리에 있어서 이론적·실무적 함의를 제시 할 수 있을 것이다.

스마트 안테나를 적용한 이동통신 시스템의 성능 분석

류은숙(Eun-Suk Ryu),이형권(Hyung-Kwun Lee),이종길(Jonggil Lee) 한국통신학회 2000 한국통신학회 학술대회논문집 Vol.2000 No.7