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Wei Li(이위),Hee-Geun Park(박희근),Young-Ran Lee(이영란),Hak-young Jang(장학영),Young Hwa Lee(이영화),Gan Li(감력),Sung-Ho Choo(추성호),Jong-Kui Jun(전종귀),Wang-Lok Lee(이왕록),Sang Ki Lee(이상기) 한국생명과학회 2012 생명과학회지 Vol.22 No.5
이 연구는 자연발생고혈압쥐(SHR)의 혈압, 골격근에서의 혈관생성 및 혈관내피세포성장인자 발현(VEGF)에 미치는 지구성운동의 효과를 조사하였다. 5주령 SHR와 WKY는 무작위로 정상혈압군(WKY, Wistar-Kyoto rat, n=9), 고혈압통제군(SHR-C, SHR Control, n=9) 및 고혈압운동군(SHR-E, SHR Exercise, n=9)으로 각각 분류하였고, 지구성운동은 트레드밀을 이용하였다(12-20 m/min, 0% grade, 60 min/day, 5 days/wk, 16 wk). 수축기혈압은 지구성운동에 의해 효과적으로 감소되었다(SHR-E vs. SHR-C, p<0.05). 골격근의 모세혈관밀도와 VEGF 단백발현은 고혈압통제군(SHR-C)이 정상혈압군(WKY)보다 모두 감소되었으나, 지구성운동(SHR-E)이 고혈압통제군(SHR-C)에 비해 모두 증가되었다. 이러한 결과들은 지구력운동 트레이닝이 SHR 골격근의 VEGF 단백발현의 증가를 통해 모세혈관밀도를 향상시키고, 이러한 모세혈관밀도의 향상이 SHR의 혈압상승을 억제할 수 있다는 것을 의미한다. This study investigated the effect of endurance exercises on blood pressure, angiogenesis, and the vascular endothelial growth factor (VEGF) expression in the skeletal muscle of spontaneously hypertensiverats (SHR). Five week old SHRs and Wistar-Kyoto rats (WKY) were randomly divided into 3 groups: Wistar-Kyoto rats (WKY, n=9), SHR control (SHR-C, n=9), and SHR endurance exercise training (SHR-E, n=9). Endurance exercise training was performed on a treadmill (12-20 m/min, 0% grade, 60 min/day, 5 days/week, 16 weeks). Systolic blood pressure was monitored with the tail-cuff method. The expression of VEGF protein and capillary density were identified using western blotting and H&E staining in the soleus muscle, respectively. Systolic blood pressure was reduced by endurance exercise in SHR (p<0.05). The capillary density of skeletal muscles in SHR-C was lower than in WKY (p<0.05), but it was recovered by endurance exercise training (SHR-E) compared to SHR-C (p<0.05), and VEGF protein was also increased by endurance exercise training compared to SHR-C (p<0.05). These data suggest that the enhancement of capillary density via an increase of VEGF expression in skeletal muscles by endurance exercise training could be an important factor to inhibit blood pressure elevation in SHR.
( Wei Li ),( Jun Hyun Jeong ),( Hee Geon Park ),( Young Ran Lee ),( Meng Li ),( Sang Ki Lee ) 한국운동영양학회 2014 Physical Activity and Nutrition (Phys Act Nutr) Vol.18 No.1
[Purpose] This study investigated the effect of endurance exercise on neointimal formation, endothelial-dependant relaxation and FOXO expression in balloon-induced carotid arteries of rats. [Methods] Male SD(Sprague-Dawley) rats of 8 weeks ages were randomly divided into 3 groups; Sham-operated control (SO, n=10), Balloon-induced control (BIC, n=10), and Balloon-induced exercise (BIE, n=10). Endurance exercise training was performed on treadmill (18 m/min, 0% grade, 60 min/day, 5 days/week, 4 weeks). [Results] Body weight is significantly reduced in BIE compared with BIC. Neointiaml formation in BIC was significantly higher than SO, but it was significantly recovered in BIE compared with BIC. Endothelial-dependent relaxation in BIC was significantly lower than SO, but it was significantly recovered in BIE compared with BIC and expression of FOXO1 and FOXO3a also were significantly increased BIE compared with BIC. [Conclusion] These data suggest that endurance exercise inhibits neointimal formation and endothelial-dependent relaxation via FOXO expression in balloon-induce atherosclerosis rat model.
( Xin Ran Li ),( Hui Xu ),( Jie Xie ),( Qiao Fu Yi ),( Wei Li ),( Dai Rong Qiao ),( Yi Cao ),( Yu Cao ) 한국미생물 · 생명공학회 2014 Journal of microbiology and biotechnology Vol.24 No.4
In order to improve the expression of heat-resistant xylanase XYNB from Aspergillus niger SCTCC 400264, XynB has been cloned into Pichia pastoris secretary vector pPIC9K. The XynB production of recombinant P. pastoris was four times that of E. coli, and the Vmax and specific activity of XynB reached 2,547.7 umol/mg and 4,757 U/mg, respectively. XynB still had 74% residual enzyme activity after 30 min of heat treatment at 80°C. From the van der Waals force analysis of XYNB (ACN89393 and AAS67299), there is one more oxygen radical in AAS67299 in their catalytic site, indicating that the local cavity is much more free, and it is more optimal for substrate binding, affinity reaction, and proton transfer, etc, and e ventually i ncreasing enzyme activity. The H-bonds analysis of XYNB indicated that there are two more H-bonds in the 33rd Ser of XYNB (AAS67299) than in the 33rd Ala(ACN89393 ), and two H-bonds between Ser70 and Asp67.
Li-Shang Dai,Cen Qian, LeiWang,Guo-Qing Wei,Qiu-Ning Liu,Yu Sun,Cong-Fen Zhang,Jun Li,Dong-Ran Liu,Bao-Jian Zhu,Chao-Liang Liu 한국응용곤충학회 2015 Journal of Asia-Pacific Entomology Vol.18 No.4
Peptidoglycan recognition proteins (PGRPs) are conserved proteins in animals from insects to mammals and play an important role in immune response by recognizing peptidoglycan on microbial surfaces. In this study, a PGRP gene from Antheraea pernyi, named Ap-PGRP-A, was isolated and characterized. Sequence analysis revealed that the full-length cDNA of Ap-PGRP-A was 961 bp, containing a 40 bp 5′-untranslated sequence, a 339 bp 3′-untranslated region and an open reading frame of 582 bp. This gene encoded a putative protein of 193 amino acid residues. Phylogenetic analysis indicated that Ap-PGRP-A had the closest protein sequence similarity to Antheraea mylitta PGRP. The recombinant protein was successfully expressed in Escherichia coli cells and a rabbit anti-Ap-PGRP-A antibody was also prepared. Real-time quantitative reverse transcription-PCR analysis showed that Ap-PGRP-A was extensively expressed in the fat body, midgut, hemocyte,malpighian tubule and epidermis of A. pernyi, especially in the fat body and midgut. The expression levels of Ap-PGRP-A were significantly up-regulated by three types of microorganisms, and Ap-PGRP-A expression was more sensitive in response to the Gram-negative bacterium E. coli than the Gram-positive bacterium Bacillus subtilis or the fungus Beauveria bassiana. These data indicate that Ap-PGRP-A may play a role in the innate immune responses of A. pernyi.
Space-Time Quantization and Motion-Aligned Reconstruction for Block-Based Compressive Video Sensing
( Ran Li ),( Hongbing Liu ),( Wei He ),( Xingpo Ma ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.1
The Compressive Video Sensing (CVS) is a useful technology for wireless systems requiring simple encoders but handling more complex decoders, and its rate-distortion performance is highly affected by the quantization of measurements and reconstruction of video frame, which motivates us to presents the Space-Time Quantization (ST-Q) and Motion-Aligned Reconstruction (MA-R) in this paper to both improve the performance of CVS system. The ST-Q removes the space-time redundancy in the measurement vector to reduce the amount of bits required to encode the video frame, and it also guarantees a low quantization error due to the fact that the high frequency of small values close to zero in the predictive residuals limits the intensity of quantizing noise. The MA-R constructs the Multi-Hypothesis (MH) matrix by selecting the temporal neighbors along the motion trajectory of current to-be-reconstructed block to improve the accuracy of prediction, and besides it reduces the computational complexity of motion estimation by the extraction of static area and 3-D Recursive Search (3DRS). Extensive experiments validate that the significant improvements is achieved by ST-Q in the rate-distortion as compared with the existing quantization methods, and the MA-R improves both the objective and the subjective quality of the reconstructed video frame. Combined with ST-Q and MA-R, the CVS system obtains a significant rate-distortion performance gain when compared with the existing CS-based video codecs.
Wei Tian,Chenyu Wang,Ran Chu,Haiyan Ge,Xiao Sun,Mingjiang Li 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00
Background Endometriosis is a common gynecological disease in women of childbearing age. Commonly used treatment methods, such as endocrine and surgical therapies, display poor therapeutic effects with a high relapse probability. Thus, novel treatments for endometriosis are required. Methods In our study, polydopamine (PDA), letrozole (LTZ), and agarose (AG) hydrogels were combined to construct an injectable hydrogel with near-infrared controlled drug delivery named LTZ-PDA@AG hydrogel for endometriosis treatment. The release of letrozole can be accurately controlled by the near-infrared light intensity, exposure duration, polydopamine concentration, and hydrogel composition. Meanwhile, we isolated endometrial stromal cells from endometrium in patients with endometriosis, and constructed the rats’ model of endometriosis to verify the biological effects of LTZ-PDA@AG hydrogel. Results Owing to the sufficiently deep penetration of near-infrared light, the LTZ-PDA@AG hydrogel displayed a high temperature increase for efficient photothermal therapy. In addition, high local temperatures can further enhance the diffusion and penetration of letrozole, thereby achieving excellent therapeutic effect in vivo. Importantly, the in vivo and vitro test demonstrated the capacity of the nanocomposite hydrogel for endocrine-photothermal synergistic therapy and the biocompatibility. Conclusion Our work proposes a novel concept for precision endometriosis therapy by photothermal-enhanced endocrine therapy for endometriosis, which is proposed for the first time for the treatment of endometriosis and demonstrates excellent potential for further clinical translation.