Microwave Ablation Treatment of Liver Cancer with a 2,450-MHz Cooled-shaft Antenna: Pilot Study on Safety and Efficacy

Jiao, De-Chao,Zhou, Qi,Han, Xin-Wei,Wang, Ya-Feng,Wu, Gang,Ren, Jian-Zhuang,Wang, Yan-Li,Ding, Peng-Xu,Ma, Ji,Fu, Ming-Ti Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.2

To evaluate efficacy of microwave ablation in a primary clinical study, sixty patients (44 men, 16 women; mean age 53 years) with 96, 1-8 cm (mean $3.20{\pm}0.17$ cm) liver cancers were treated with 2,450-MHz internally cooled-shaft antenna. Complete ablation (CA) and local tumor progression (LTP) rates as well as complications were determined. CA rates in small (< 3.0 cm), intermediate (3.1-5.0 cm) and large (5.1-8.0 cm) liver cancers were 96.4% (54/56), 92.3% (24/26) and 78.6% (11/14), respectively. During a mean follow-up period of $17.17{\pm}6.52$ months, LTP occurred in five (5.21%) treated cases. There was no significant difference in the CA and LTP rates between the HCC and liver metastasis patient subgroups (P<0.05). Microwave ablation provides a reliable, efficient, and safe technique to perform hepatic tumor ablation.

Genetic Diversity and Geographical Differentiation of Desmodium triflorum (L.) DC. in South China Revealed by AFLP Markers

Mao-feng Yue,Ren-chao Zhou,Ye-lin Huang,Guo-rong Xin,Su-hua Shi,Li Feng 한국식물학회 2010 Journal of Plant Biology Vol.53 No.2

High levels of genetic variation enable species to adapt to changing environments and provide plant breeders with the raw materials necessary for artificial selection. In the present study, six AFLP primer pairs were used to assess the genetic diversity of Desmodium triflorum (L.)DC. from 12 populations in South China. A high percentage of polymorphic loci (P=76.16%) and high total gene diversity (HT=0.310) were found, indicating that the genetic diversity of D. triflorum is high at the species level. Genetic diversity was also relatively high at the population level (P=55.85%, He=0.230). The coefficient of gene differentiation among populations (GST) was 0.255, indicating that while most genetic diversity resided within populations, there was also considerable differentiation among populations. AMOVA also indicated 24.29% of the total variation to be partitioned among populations (ΦST=0.243). UPGMA clustering analysis based on genetic distances showed that the 12 populations could be separated into three subgroups: an eastern, a western, and a central-southern subgroup. However, a Mantel test revealed no significant correlation (r=0.286, p=0.983)between the geographical distances and genetic distances separating these populations; mountain barriers to gene flow and human disturbance may have confounded these correlations. The present study has provided some fundamental genetic data that will be of use in the exploitation of D. triflorum.

The upregulation of keratocan promotes the progression of human pancreatic cancer

Gao Huijie,Qian Ruikun,Ren Qiang,Zhang Litao,Qin Wei,Zhou Caiju,Wang Huiyun,Liu Chao,Zhang Yuntao 대한독성 유전단백체 학회 2024 Molecular & cellular toxicology Vol.20 No.2

Background The role of the extracellular matrix (ECM) in oncogenic contexts has been studied previously, but the expression patterns and functional role of keratocan, a classical small leucine-rich proteoglycan found in the ECM, in tumors remain poorly understood. As pancreatic cancer (PC) is characterized by desmoplasia in the ECM, this study sought to assess the significance of keratocan in PC. Objective In this study, Gene Expression Profiling Interactive Analysis (GEPIA) was first used to analyze the expression pattern of keratocan in PC. Keratocan, P53, and P21 levels were evaluated in PDAC patient tissues and the role of keratocan was additionally directly assessed via transfecting PDAC cell lines with a pENTER-human keratocan construct. Results Patients with PC showing high levels of keratocan had low survival rates. A significantly upregulated expression of keratocan was observed in the PC tumor tissues in comparison to healthy paracancerous tissues. Keratocan upregulation in BxPC-3 and PANC-1 cells markedly enhanced their proliferation and migration, but reduced cellular apoptosis. Furthermore, the P53 and P21 expression levels were significantly reduced in pancreatic ductal adenocarcinoma cells overexpressing keratocan. P53 and P21 were downregulated in PC tumor tissues. Conclusions All results showed that keratocan played important roles in the promoting of PC. Background The role of the extracellular matrix (ECM) in oncogenic contexts has been studied previously, but the expression patterns and functional role of keratocan, a classical small leucine-rich proteoglycan found in the ECM, in tumors remain poorly understood. As pancreatic cancer (PC) is characterized by desmoplasia in the ECM, this study sought to assess the significance of keratocan in PC. Objective In this study, Gene Expression Profiling Interactive Analysis (GEPIA) was first used to analyze the expression pattern of keratocan in PC. Keratocan, P53, and P21 levels were evaluated in PDAC patient tissues and the role of keratocan was additionally directly assessed via transfecting PDAC cell lines with a pENTER-human keratocan construct. Results Patients with PC showing high levels of keratocan had low survival rates. A significantly upregulated expression of keratocan was observed in the PC tumor tissues in comparison to healthy paracancerous tissues. Keratocan upregulation in BxPC-3 and PANC-1 cells markedly enhanced their proliferation and migration, but reduced cellular apoptosis. Furthermore, the P53 and P21 expression levels were significantly reduced in pancreatic ductal adenocarcinoma cells overexpressing keratocan. P53 and P21 were downregulated in PC tumor tissues. Conclusions All results showed that keratocan played important roles in the promoting of PC.

Numerical Simulation and Measurement of Welding Residual Stresses in Orthotropic Steel Decks Stiffened with U-Shaped Ribs

Ying Gu,Yadong Li,Zihao Zhou,Songbo Ren,Chao Kong 한국강구조학회 2020 International Journal of Steel Structures Vol.20 No.3

This study aims to investigate the magnitude and distribution of welding residual stresses in orthotropic steel decks stiff ened with U-shaped ribs. Numerical simulation of the welding residual stresses is performed using the thermal elastic–plastic fi nite element method. The residual stresses are subsequently measured using the hole-drilling strain-gage method. Based on the results of this study, it can be determined that: (1) The longitudinal welding residual stresses ( σ z ) in the weld zones are tensile stresses, where the peak value exceeds the minimum yield strength ( f yl ); however, relatively low compressive stresses exist in the areas located far away from the welds; (2) The stress gradient in the weld zone is steep, and the widths of the areas experiencing tensile stress are narrow; (3) In the case of the upper surface of the deck, the weld that is fi rst deposited has the highest peak stress, while similar stresses are determined for the remaining welds. In the case of the lower surface of the deck, the peak stress in the weld zones is greater than that on the upper surface; (4) In the case of the weld zone of the U-shaped ribs, the tensile stresses reach up to 1.15 f yl ; however, at locations a short distance away from the welds, the σ z varies from tensile to compressive, and fi nally transforms into a small tensile stress at the base of the U-shaped ribs. In addition, the simulated and measured σ z are compared, and the deviation between them is analyzed.

Induced Abortion and Breast Cancer: Results from a Population-Based Case Control Study in China

Wu, Jun-Qing,Li, Yu-Yan,Ren, Jing-Chao,Zhao, Rui,Zhou, Ying,Gao, Er-Sheng Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Aim: To determine whether induced abortion (IA) increases breast cancer (BC) risk. Materials and Methods: A population-based case-control study was performed from Dec, 2000 to November, 2004 in Shanghai, China, where IA could be verified through the family planning network and client medical records. Structured questionnaires were completed by 1,517 cases with primary invasive epithelial breast cancer and 1,573 controls frequency-matched to cases for age group. The information was supplemented and verified by the family planning records. Statistical analysis was conducted with SAS 9.0. Results: After adjusting for potential confounders, induced abortions were not found to be associated with breast cancer with OR=0.94 (95%CI= 0.79-1.11). Compared to parous women without induced abortion, parous women with 3 or more times induced abortion (OR=0.66, 95%CI=0.46 to 0.95) and women with 3 or more times induced abortion after the first live birth (OR=0.66, 95%CI =0.45 to 0.97) showed a lower risk of breast cancer, after adjustment for age, level of education, annual income per capita, age at menarche, menopause, parity times, spontaneous abortion, age at first live birth, breast-feeding, oral contraceptives, hormones drug, breast disease, BMI, drinking alcohol, drinking tea, taking vitamin/calcium tablet, physical activity, vocation, history of breast cancer, eating the bean. Conclusions: The results suggest that a history of induced abortions may not increase the risk of breast cancer.

A Noninjection Reaction Route to CuInSe₂ Nanocrystals with Triethanolamine as the Complexing Agent

Liu, Wen-Long,Wu, Meng-Qiang,Zhou, Ru-Chao,Yan, Li-Dan,Zhang, Shu-Ren,Zhang, Qi-Yi Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

The chalcopyrite-type $CuInSe_2$ is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like $CuInSe_2$ nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase $CuInSe_2$ nanocrystals at $240^{\circ}C$. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized $CuInSe_2$ nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained $CuInSe_2$ nanocrystals was $1.03{\pm}0.03$ eV.

PCR-mediated Recombination of the Amplification Products of the Hibiscus tiliaceus Cytosolic Glyceraldehyde-3-phosphate Dehydrogenase Gene

( Ling Hui Wu ),( Tian Tang ),( Ren Chao Zhou ),( Su Hua Shi ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.2

The Antibiosis Action and Rice-Induced Resistance, Mediated by a Lipopeptide from Bacillus amyloliquefaciens B014, in Controlling Rice Disease Caused by Xanthomonas oryzae pv. oryzae

( Shu Bin Li ),( Shi Ru Xu ),( Rui Ning Zhang ),( Yuan Liu ),( Ren Chao Zhou ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.4

In the present study, a lipopeptide (named AXLP14) antagonistic to Xanthomonas oryzae pv. oryzae (Xoo) was obtained from the culture supernatant of Bacillus amyloliquefaciens B014. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis demonstrated that AXLP14 consisted of surfactin homologs. The minimum inhibition concentration and minimum bactericidal concentration of AXLP14 against Xoo were determined to be 1.25 and 2.50 mg/ml, respectively. At a concentration of 0.613 mg/ml, AXLP14 strongly inhibited the formation of Xoo biofilm. AXLP14 also inhibited the motility of Xoo in a concentration-dependent manner. Applying AXLP14 to rice seedlings significantly reduced the incidence and severity of disease caused by Xoo. In Xoo-infected rice seedlings, AXLP14 strongly and continuously up-regulated the expression of both OsNPR1 and OsWRKY45. In addition, AXLP14 effectively inhibited the Xoo-induced up-regulation of the expression of the abscisic acid biosynthesis gene OsNECD3 and the abscisic acid signalingresponsive gene OsLip9, indicating that AXLP14 may protect rice against Xoo-induced disease by enhancing salicylic acid defense and interfering with the abscisic acid response to virulence.

A Noninjection Reaction Route to CuInSe2 Nanocrystals with Triethanolamine as the Complexing Agent

Qi-Yi Zhang,Wen-Long Liu,Meng-Qiang Wu,Ru-Chao Zhou,Li-Dan Yan,Shu-Ren Zhang 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

The chalcopyrite-type CuInSe_2 is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like CuInSe_2 nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase CuInSe_2 nanocrystals at 240 ^oC. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized CuInSe_2 nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained CuInSe_2nanocrystals was 1.03 ± 0.03 eV.