효소 Lipoxygenase의 신규기질 : Acylglycerol, acylethanolamide, lysophospholipids 및 phospholipids

황룡쌍,류희문,박천호,석대은 충남대학교 약학대학 의약품개발연구소 2007 藥學論文集 Vol.22 No.-

Lipoxygenase belongs to a diverse family of nonheme ferroproteins that oxygenate polyenoic fatty acids containing 1,4-pentadiene structure to form their corresponding hydroperoxy derivatives. Lipoxygenases (LOXs), widely distributed in animals and plants, have a key function in the formation of biologically active substances from pulyunsaturated fatty acids. Generally, free polyunsaturated fatty acids, liberated from membrane phospholipids via phospholipase-catalyzed hydrolysis, are used as substrates for LOXs. Although it is acknowledged that free polyunsaturated fatty acids are preferred to phospholipids or triglycerides as substrates, there have been recent reports that mammalian enzymes can oxidize certain phospholipids. Especially, reticulocyte LOX (15-LOX) leukocyte 15-LOX, leukocyte LOX (12-LOX) can oxygenate complex substrates such as phospholipids and biomembranes. In addition, acylglycerol and acylethanolamide are utilized by lipoxygeanse as well as cycoloxygenase; the latter enzyme contributes to generation of bioactive prostanoids derivative. Furthermore, lysophosphatidylcholine or lysophosphatidic acid containing linoleoyl or arachidonoyl moieties are known to be oxygenated by reticulocyte LOX, leukocyte 15-LOX or leukocyte-type 12-LOX; oxygenated lysophospholipids can play a carrier role in transporting oxygenated derivatives. Thus, the use of various lipid substrates as new substrates for lipoxygenase may extend the physiological roles of those lipids containing unsaturated fatty acyl chains.

Synchronous immobilization and conversion of polysulfides on a VO₂-VN binary host targeting high sulfur load Li-S batteries

Song, Yingze,Zhao, Wen,Kong, Long,Zhang, Li,Zhu, Xingyu,Shao, Yuanlong,Ding, Feng,Zhang, Qiang,Sun, Jingyu,Liu, Zhongfan The Royal Society of Chemistry 2018 ENERGY AND ENVIRONMENTAL SCIENCE Vol.11 No.9

<P>Lithium-sulfur (Li-S) batteries are deemed as one of the most promising next-generation energy storage systems. However, their practical application is hindered by existing drawbacks such as poor cycling life and low Coulombic efficiency due to the shuttle effect of lithium polysulfides (LiPSs). We herein present an <I>in situ</I> constructed VO2-VN binary host which combines the merits of ultrafast anchoring (VO2) with electronic conducting (VN) to accomplish smooth immobilization-diffusion-conversion of LiPSs. Such synchronous advantages have effectively alleviated the polysulfide shuttling, promoted the redox kinetics, and hence improved the electrochemical performance of Li-S batteries. As a result, the sulfur cathode based on the VO2-VN/graphene host exhibited an impressive rate capability with ∼1105 and 935 mA h g<SUP>−1</SUP> at 1C and 2C, respectively, and maintained long-term cyclability with a low capacity decay of 0.06% per cycle within 800 cycles at 2C. More remarkably, favorable cyclic stability can be attained with a high sulfur loading (13.2 mg cm<SUP>−2</SUP>). Even at an elevated temperature (50 °C), the cathodes still delivered superior rate capacity. Our work emphasizes the importance of immobilization-diffusion-conversion of LiPSs toward the rational design of high-load and long-life Li-S batteries.</P>

A Noninjection Reaction Route to CuInSe₂ Nanocrystals with Triethanolamine as the Complexing Agent

Liu, Wen-Long,Wu, Meng-Qiang,Zhou, Ru-Chao,Yan, Li-Dan,Zhang, Shu-Ren,Zhang, Qi-Yi Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

The chalcopyrite-type $CuInSe_2$ is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like $CuInSe_2$ nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase $CuInSe_2$ nanocrystals at $240^{\circ}C$. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized $CuInSe_2$ nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained $CuInSe_2$ nanocrystals was $1.03{\pm}0.03$ eV.

A study on perceived advertising value and attitude toward mobile advertising

Wen-Long, Liu(유문용),Hyeong-Yu, Jang(장형유),Seo-Yoon, Jung(정서윤) 대한경영학회 2012 대한경영학회 학술발표대회 발표논문집 Vol.2012 No.2

In order to perform mobile marketing activities more effectively, mobile advertising is considered to be a continuous area for research. As one of the issues, attitudes toward advertising have been a focus of attention for a long time. Previous studies verified and emphasized that consumer attitude toward mobile advertising is significantly affected by the perceived entertainment, informativeness and credibility of an advertisement causing the way consumers evaluate the value of it. However, the general attitude toward advertising manifests itself not only through perceived advertisement value but also through some factors from implementation process and consumers themselves such as the form of advertisement, the agreement between company and consumer, the involvement consumers pay to mobile advertising, their experience and the type of mobile phone they have. To cast light on the role of company-factors and consumer-factors, the following sub questions will be explored empirical research: 1. How consumers perceived mobile advertising value is affected by company-factors or consumer-factors? 2. Does any company-factor or consumer-factor have a moderating effect between consumers perceived mobile advertising value and advertising attitude? According to a t-test comparative analysis, we found that all company-factors and consumer-factors affect the consumers perceived mobile advertising value positively. On the other hand, the result of hierarchical regression analysis reveal that, among company-factors, incentive is the most attractive to consumers as expected. Among consumer-factors, the level of involvement leads different attitudes. And, smart phone users are more accustomed to various forms of mobile advertisement and hold optimistic attitudes than 2G phone users. As well, different forms of advertisement give different information of product and different visual experience which makes moderating effect on attitude to a certain degree. Finally, as people become more and more familiar with the mobile advertising, prior permission and experience seem less important.

Characteristics, Prognostic Factors, and Survival of Patients with NK/T-Cell Lymphoma of Non-upper Aerodigestive Tract: A 17-Year Single-Center Experience

Ze-Long Liu,Xi-Wen Bi,Xue-Wen Zhang,De-Xin Lei,Pan-Pan Liu,Hang Yang,Yan Gao,Yuan-Xue Jiang,Wen-Qi Jiang,Yi Xia 대한암학회 2019 Cancer Research and Treatment Vol.51 No.4

Purpose The extranodal natural killer (NK)/T-cell lymphoma (NKTCL) of non-upper aerodigestive tract (NUAT) was found to have clinical heterogeneity compared with NKTCL of the upper aerodigestive tract (UAT) in small scale studies. We conducted this study in a much larger cohort to analyze the clinical characteristics, prognostic factors, treatment modality, and clinical outcomes of patients with NUAT-NKTCL. Materials and Methods From January 2001 to December 2017, a total of 757 NKTCL patients were identified and included in this study, including 92 NUAT-NKTCL patients (12.2%) and 665 UAT-NKTCL patients (87.8%). Results NUAT-NKTCL patients had relatively poorer performance status, more unfavorable prognostic factors, and more advanced stage, compared with UAT-NKTCL patients. The 5-year overall survival (OS) was 34.7% for NUAT-NKTCL, which was significantly worse than UAT-NKTCL (64.2%, p < 0.001). The median OS duration was 30.9 months for NUAT-NKTCL. Multivariate analysis showed that presence with B symptoms and elevated serum lactate dehydrogenase independently predicted worse OS. International prognostic index score and prognostic index of NK lymphoma score still had prognostic values in NUAT-NKTCL, while the Ann Arbor system could not accurately predict the OS. Conclusion NUAT-NKTCL is a distinctive subtype of NKTCL in many aspects. Patients with NUAT-NKTCL have relatively poorer performance status, more unfavorable prognostic factors, more advanced stage, and poorer prognosis.

라이프스타일에 따른 스마트폰 이용 동인에 관한 연구

유문용(Wen-Long Liu),장형유(Hyeong-Yu Jang) 한국인터넷전자상거래학회 2012 인터넷전자상거래연구 Vol.12 No.2

The purpose of this study is to analyze lifestyles of smartphone users and discuss the motivations of users using smartphones according to their life styles. Specifically, this study categorizes types of smartphone users in line with features of their lifestyles related to food, clothing, shelter, and cultural life while looking into those differences. In addition, this paper examines what leads people to use smartphones by individual type of each lifestyle; based on this research, we suggest future marketing strategies to segment the smartphone users and potential consumers and develop new smartphone devices, services, applications and many others by referring to their usage motivations.

A study on perceived advertising value and attitude toward mobile advertising : Focus on the effect of moderators

유문용(Wen Long Liu),장형유(Hyeong Yu Jang),조염(Yan Zhao),이한경(Han Kyoung Lee) 한국창업학회 2012 한국창업학회 Conferences Vol.2012 No.-

In order to perform mobile marketing activities more effectively, marketers and researchers pay more and more attention to mobile advertising which is considered to be an important marketing tool and continuous area for research. As one of the issues, attitudes toward advertising have been a focus of attention for a long time. Previous studies verified and emphasized that consumer attitude toward mobile advertising is significantly affected by the perceived entertainment, informativeness and credibility of an advertisement causing the way consumers evaluate the value of it. However, the general attitude toward advertising manifests itself not only through perceived advertisement value but also through some factors from implementation process and consumers themselves such as the form of advertisement, the agreement between company and consumer, the involvement consumers pay to mobile advertising and their experience of using mobile advertisement for shopping.. To cast light on the role of company-factors and consumer-factors, the following sub questions will be explored empirical research : 1. How different are consumers perceived mobile advertising value according to company-factors or consumer-factors? 2. Does any company-factor or consumer-factor have a moderating effect between consumers perceived mobile advertising value and advertising attitude? According to a t-test comparative analysis, we found that all company-factors and consumer-factors affect the consumer s perceived mobile advertising value positively. The result of hierarchical regression analysis reveals that, all of the moderator have moderating effect between perceived credibility and advertising attitude. Specially, among company-factors, incentive is the most attractive to consumers as expected. Prior permission is playing a role as always. As well, different forms of advertisement give different information of product and different visual experience which makes moderating effect on attitude to a certain degree. On the other hand, the result shows that both of two consumer-factors have a significant moderating effect between consumers perceived credibility and advertising attitude. Consistent with common sense, the level of involvement and the using experience always lead people to evaluate some product or service differently.

β-elemene Induces Caspase-dependent Apoptosis in Human Glioma Cells in vitro through the Upregulation of Bax and Fas/FasL and Downregulation of Bcl-2

Li, Chen-Long,Chang, Liang,Guo, Lin,Zhao, Dan,Liu, Hui-Bin,Wang, Qiu-Shi,Zhang, Ping,Du, Wen-Zhong,Liu, Xing,Zhang, Hai-Tao,Liu, Yang,Zhang, Yao,Xie, Jing-Hong,Ming, Jian-Guang,Cui, Yu-Qiong,Sun, Ying Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.23

Background: ${\beta}$-elemene, extracted from herb medicine Curcuma wenyujin has potent anti-tumor effects in various cancer cell lines. However, the activity of ${\beta}$-elemene against glioma cells remains unclear. In the present study, we assessed effects of ${\beta}$-elemene on human glioma cells and explored the underlying mechanism. Materials and Methods: Human glioma U87 cells were used. Cell proliferation was determined with MTT assay and colony formation assay to detect the effect of ${\beta}$-elemene at different doses and times. Fluorescence microscopy was used to observe cell apoptosis with Hoechst 33258 staining and change of glioma apoptosis and cell cycling were analyzed by flow cytometry. Real-time quantitative PCR and Western-blotting assay were performed to investigated the influence of ${\beta}$-elemene on expression levels of Fas/FasL, caspase-3, Bcl-2 and Bax. The experiment was divided into two groups: the blank control group and ${\beta}$-elemne treatment group. Results: With increase in the concentration of ${\beta}$-elemene, cytotoxic effects were enhanced in the glioma cell line and the concentration of inhibited cell viability ($IC_{50}$) was $48.5{\mu}g/mL$ for 24h. ${\beta}$-elemene could induce cell cycle arrest in the G0/G1 phase. With Hoechst 33258 staining, apoptotic nuclear morphological changes were observed. Activation of caspase-3,-8 and -9 was increased and the pro-apoptotic factors Fas/FasL and Bax were upregulated, while the anti-apoptotic Bcl-2 was downregulated after treatment with ${\beta}$-elemene at both mRNA and protein levels. Furthermore, proliferation and colony formation by U87 cells were inhibited by ${\beta}$-elemene in a time and does-dependent manner. Conclusions: Our results indicate that ${\beta}$-elemene inhibits growth and induces apoptosis of human glioma cells in vitro. The induction of apoptosis appears to be related with the upregulation of Fas/FasL and Bax, activation of caspase-3,-8 and -9 and downregulation of Bcl-2, which then trigger major apoptotic cascades.

The kinematic design of a planar-cam type pick-and-place device

Wen-Tung Chang,Long-Iong Wu,Chun-Hsien Liu 대한기계학회 2008 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.22 No.12

The planar-cam type pick-and-place device can clearly and effectively achieve the desired curvilinear motion of its end effector, and it can be designed and fabricated easily. By employing the concept of velocity instant center, the cam profiles, the paths of cutters, the pressure angles and the radii of curvature of the dual cams of the planar-cam type pickand- place device can be expressed parametrically. The cam profiles may have concave portions, and each minimum radius of curvature of the concave portion of the dual cam profiles is the upper bound of the grinding-wheel radius that may not cause undercutting.

Correlation Study of Knowledge and Behavior Regarding Breast Care among Female Undergraduate Students in China

Liu, Meng-Xue,Li, Jian,Geng, Yun-Long,Wang, Yan-Chun,Li, Jie,Chen, Yu-Juan,Ali, Gholam,Tarver, Siobhan L.,Wen, Yu-Feng,Sun, Wen-Jie Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Background: This study aimed to understand the relationship between knowledge level and behavior on breast care in Chinese students, so as to provide strategies for improving the health education of breast care and subsequently for aiding in breast cancer prevention. Materials and Methods: A self-designed questionnaire was used to evaluate breast care knowledge level and characterize related behavior. Correlation analysis was conducted for the knowledge level and behavior. The study was carried out using 597 female undergraduate students in medical and non-medical colleges in Wuhu, China. Results: The average score of breast care knowledge was $5.32{\pm}1.68$ ($5.62{\pm}1.68$ and $5.00{\pm}1.68$ for medical and non-medical students, respectively), with a greater score value for sophomores ($5.59{\pm}1.72$) than freshmen ($5.18{\pm}1.65$). The average score of breast care behavior was $2.21{\pm}1.13$, again with a greater value in sophomores ($2.37{\pm}1.15$) than freshmen ($2.21{\pm}1.13$). A significant positive correlation (r=0.231, p<0.01) between knowledge scores and behavior scores was observed. In addition, various factors, including paying attention to breast care information, receiving breast self-examination guidance, TV program and Internet, were found to influence breast care knowledge. Conclusions: In general, female undergraduate students lack of self-awareness of breast care with a low rate of breast self-examination. It is necessary to carry out health education to improve early detection of breast cancer.