A Novel Facile and Green Synthesis Protocol to Prepare High Strength Regenerated Silk Fibroin/SiO2 Composite Fiber

Qiang Liu,Zhaohui Meng,Ronghui Wu,Liyun Ma,Wu Qiu,Honghao Zhang,Shuihong Zhu,Lingqing Kong,Zijjie Xu,Aniruddha Patil,Xiangyang Liu 한국섬유공학회 2019 Fibers and polymers Vol.20 No.10

In this work, regenerated silk fibroin (RSF) and silicon dioxide (SiO2) composite fiber was successfully extrudedby wet spinning method. The effect of SiO2 addition on structure of the composite fiber at microscopic level is studied, whichsubsequently correlated to the mechanical performance. The best concentration ratio for composite fiber is identified byscreening SiO2 concentration from 0.025 w/w% to 0.5 w/w%. The experimental results revealed that the SiO2 at a lowconcentration of 0.1 w/w% was well distributed. The breaking stress, breaking strain and Young’s modulus at 0.1 w/w% SiO2addition of the RSF fibers increased considerably compared to the neat RSF fibers from 243±3 to 458±21 MPa, 51±4 % to54±7 % and 6.34±0.55 to 11.69±1.12 GPa, respectively. To the best of our knowledge, this is the first report for RSF/SiO2composite fiber. We believed the insight provided in this report which looks into the structural evolution should be beneficialto the future design and building of other advanced functional fibers.

Genetic and phylogenetic analysis of the honey bee sacbrood virus from jiangxi isolates

Meng Yaping,Yu Xinyue,Huang Qiang,Zhang Li Zhen,Wu Xiaobo,Wang Zilong,Yan Wei Yu 한국응용곤충학회 2022 Journal of Asia-Pacific Entomology Vol.25 No.1

The high prevalence of honeybee viral diseases poses a severe threat to the health of honeybees and causes substantial economic losses worldwide. Sacbrood virus (SBV) is a single-strand RNA virus that infects honeybees at all life stages. The infection can shorten the lifespan of adult bees and is lethal to larvae. SBV is the major cause of honeybee losses in Asia. Genetic and phylogenetic analyses of SBV isolates from different areas have been previously conducted. However, the impact of Apis mellifera Linnaeus and Apis cerana Fabricius coexistence on the infection and phylogeny of SBV remains unknown. In this study, we collected A. cerana and A. mellifera samples from commercial apiaries, only A. cerana in mountainous region. SBV prevalence was evaluated in three commercial apiaries of Jinxi, Tonggu and Nanchang and two mountainous regions of Zixi and Yifeng. In our sampling location, we found a higher SBV prevalence in the mountainous regions than in commercial apiaries. Partial structural polyprotein coding sequences were sequenced and compared with other GenBank SBV isolates. Phylogenetic tree topologies showed that SBV isolates form two major groups based on their host specificity, and isolates from same country tend to cluster together in subclades, indicating that the host and geographic region has significant effects on SBV strain specificity.

Meta-Analysis of the Association between H63D and C282Y Polymorphisms in HFE and Cancer Risk

Zhang, Meng,Xiong, Hu,Fang, Lu,Lu, Wei,Wu, Xun,Wang, Yong-Qiang,Cai, Zhi-Ming,Wu, Song Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.11

Background: Previous studies suggested that the H63D and C282Y polymorphisms in the HFE genes were susceptible to many cancer types, nevertheless, the present results were inconclusive. Thus, the present study was aimed to evaluate the association between the HFE polymorphisms (H63D and C282Y) and cancer risk via meta-analysis. Materials and Methods: We retrieved PubMed, Google Scholar, Embase and Web of Science databases for all eligible studies up to April 1, 2015. All the statistical analysis was conducted by STATA 12.0. Results: Finally, a total of 20 publications including 24 case-control studies, comprising 6,524 cases and 31,080 controls for HFE-C282Y polymorphism and 19 publications including 21 case control studies, comprising 5,648 cases and 14,257 controls for HFE-H63D polymorphism were enrolled in our analysis. An increased risk for overall cancer risk was identified in HFE-H63D polymorphism under allele contrast (D vs H: OR=1.153; 95%CI=1.031-1.289, Pheterogeneity=0.002), homozygotes vs wide type (DD vs HH: OR=1.449; 95%CI=1.182-1.777, Pheterogeneity=0.391), dominant model (DD+HD vs HH: OR=1.145; 95%CI=1.007-1.301, Pheterogeneity=0.002) and recessive model (DD vs HD+HH: OR=1.416 ; 95%CI=1.156-1.735, Pheterogeneity=0.549), as well as HFE-C282Y under homozygotes vs wide type (YY vs CC: OR=1.428, 95%CI=1.017-2.006, Pheterogeneity=0.220). In addition, in the stratified analysis by cancer type, an increased risk was identified in hepatocellular carcinoma and breast cancer in C282Y polymorphism, as well as pancreatic cancer in H63D polymorphism, whereas a decreased risk of colorectal cancer was identified in C282Y polymorphism. Conclusions: Present study suggested that H63D and C282Y polymorphisms associated with an increased risk of overall cancer. Nevertheless, well-designed study with large sample size will be continued on this issue of interest.

Paraoxonase 1 (PON1) Q192R Gene Polymorphism and Cancer Risk: A Meta-Analysis Based on 30 Publications

Zhang, Meng,Xiong, Hu,Fang, Lu,Lu, Wei,Wu, Xun,Huang, Zhan-Sen,Wang, Yong-Qiang,Cai, Zhi-Ming,Wu, Song Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.10

Common genetic variation Q192R in the paraoxonase 1 (PON1) gene has been considered to be implicated in the development of many cancers. Nevertheless, results from the related studies were inconsistent. To elucidate the association, we performed a meta-analysis for 8,112 cases and 10,037 controls from 32 published case-control studies. Odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the strength of the association by STATA 12.0 software. Overall, we revealed that the PON1-192R allele was associated with a reduced risk of the overall cancers. Moreover, in the stratified analysis by cancer types (breast cancer, prostate cancer, brain cancer etc.), the results showed that PON1-192R allele was associated with a decreased risk in breast cancer (R vs Q: OR=0.605, 95% CI=0.378-0.967, $P_{heterogeneity}=0.000$; RR vs QQ: OR=0.494, 95% CI=0.275-0.888, $P_{heterogeneity}=0.002$; RQ vs QQ: OR=0.465, 95% CI=0.259-0.835, $P_{heterogeneity}=0.000$; and RR+RQ vs QQ: OR=0.485, 95% CI=0.274-0.857, $P_{heterogeneity}=0.000$), and associated with prostate cancer in homozygote (RR vs QQ: OR=0.475, 95% CI=0.251-0.897, $P_{heterogeneity}=0.001$) and recessive models (RR vs RQ+QQ: OR=0.379, 95% CI=0.169-0.853, $P_{heterogeneity}=0.000$), while an increased risk was identified in lymphoma (R vs Q: OR=1.537, 95% CI=1.246-1.896, $P_{heterogeneity}=0.944$; RR vs QQ: OR=2.987, 95% CI=1.861-4.795, $P_{heterogeneity}=0.350$; RR+RQ vs QQ: OR=1.354, 95% CI=1.021-1.796, $P_{heterogeneity}=0.824$; and RR vs RQ+QQ: OR=2.934, 95% CI=1.869-4.605, $P_{heterogeneity}=0.433$), and an increased risk in prostate cancer under heterozygote comparison (RQ vs QQ: OR=1.782, 95% CI=1.077-2.950, $P_{heterogeneity}=0.000$) and dominant models (RR+RQ vs QQ: OR=1.281, 95% CI=1.044-1.573, $P_{heterogeneity}=0.056$). When subgroup analysis that performed by the control source (hospital based or population based), a decreased risk of the overall cancers was revealed by homozygote (RR vs QQ: OR=0.601, 95% CI=0.366-0.987, $P_{heterogeneity}=0.000$) and dominant models (RR vs RQ+QQ: OR= 0.611, 95% CI=0.384-0.973, $P_{heterogeneity}=0.000$) in hospital based group. Stratifying by ethnicity, a significantly reduced risk of the overall cancers under allele contrast model (R vs Q: OR=0.788, 95% CI=0.626-0.993, $P_{heterogeneity}=0.000$) was uncovered in Caucasian. In summary, these findings suggested that PON1 Q192R polymorphism was associated with a reduced risk of the overall cancers, nevertheless, it might increase cancer susceptibility of prostate and lymphoma risk. Large well-designed epidemiological studies will be continued on this issue of interest.

Mechanism underlying 2n male and female gamete formation in lemon via cytological and molecular marker analysis

Kai‑Dong Xie,Qiang‑Ming Xia,Jun Peng,Xiao‑Meng Wu,Zong‑Zhou Xie,Chun‑Li Chen,Wen‑Wu Guo 한국식물생명공학회 2019 Plant biotechnology reports Vol.13 No.2

The unreduced (2n) gametes have long been used in triploid breeding of citrus. In lemon, the previously reported mechanisms for 2n megagametophyte formation were controversial, whereas that for 2n pollen production is still unknown. Herein, the frequency of and mechanism underlying 2n megagametophyte and 2n pollen formation in ‘Eureka’ lemon were investigated based on cytological observation and genotyping of the triploid hybrids between ‘Eureka’ lemon and ‘Early gold’ sweet orange. As a result, 4.79% of the viable pollens of ‘Eureka’ lemon were identified as the 2n pollen with a larger diameter (70.16 ± 3.92 μm). The 2n pollen might be resulted from the formation of parallel spindles at meiosis stage II. Among the 204 plantlets regenerated from embryo rescue following the sexual cross, 12 were triploids as identified by flow cytometry. According to the analysis of heterozygosity transmission using 13 pericentromeric single nucleotide polymorphism (SNP) markers and 20 randomly distributed simple sequence repeat (SSR) markers, 11 triploids were identified to be originated from the fertilization of 2n megagametophytes of ‘Eureka’ lemon, with a frequency of 5.39%. Among them, nine 2n megagametophytes were supposed to be arisen from the second division restitution (SDR), whereas the other two were from postmeiotic genome doubling (PMD). These results to understand the mechanism underlying 2n gamete formation in lemon are valuable for its efficient polyploid breeding.

Asparagine Residue at Position 71 is Responsible for Alkali - Tolerance of the Xylanase from Bacillus pumilus A - 30

(Xiang Mei Liu),(Meng Qi),(Jian Qiang Lin),(Zhi Hong Wu),(Yin Bo Qu) 한국미생물생명공학회 2001 Journal of microbiology and biotechnology Vol.11 No.3

N/A N/A

A Noninjection Reaction Route to CuInSe₂ Nanocrystals with Triethanolamine as the Complexing Agent

Liu, Wen-Long,Wu, Meng-Qiang,Zhou, Ru-Chao,Yan, Li-Dan,Zhang, Shu-Ren,Zhang, Qi-Yi Korean Chemical Society 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

The chalcopyrite-type $CuInSe_2$ is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like $CuInSe_2$ nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase $CuInSe_2$ nanocrystals at $240^{\circ}C$. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized $CuInSe_2$ nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained $CuInSe_2$ nanocrystals was $1.03{\pm}0.03$ eV.

Initial fault time estimation of rolling element bearing by backtracking strategy, improved VMD and infogram

Abdalla Babiker,Changfeng Yan,Qiang Li,Jiadong Meng,Lixiao Wu 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.2

Rolling bearing failure is widely regarded as a failure form of industrial machines. Owing to the poor operating circumstance with the stochastic contact between rolling elements, the performance of the bearing will deteriorate over time and cause a cascade breakdown in the mechanical system. Early fault detection has been found to be an effective strategy to avoid economic loss. Therefore, an integration method for fault diagnosis that combines backtracking strategy, improved variational mode decomposition (VMD), and infogram is proposed to tackle the challenge of the early feature extraction from the heavy noisy non-stationary signal. The backtracking strategy is adopted to track the data sample points earlier than the fault threshold determined based on the kurtosis index. The optimum parameters α and K of VMD are acquired through the particle swarm optimization (PSO) algorithm. In this way, the more accurate intrinsic mode functions (IMFs) can be gained by the improved VMD. The optimum IMFs are acquired according to the maximum values of kurtosis and correlation coefficients, and these IMFs can be reconstructed into the noise reduction signal. Since envelope analysis requires the selection of the appropriate central frequency and bandwidth, infogram is utilized to select the values of them. A simulated case is applied to demonstrate the validation of the proposed method. And to further illustrate its practicality, it is employed to perform early fault diagnosis for an experimental case. According to the diagnosis results, the proposed method has conspicuous superiority over the other existing technologies for estimating incipient fault time of the bearing.

A Noninjection Reaction Route to CuInSe2 Nanocrystals with Triethanolamine as the Complexing Agent

Qi-Yi Zhang,Wen-Long Liu,Meng-Qiang Wu,Ru-Chao Zhou,Li-Dan Yan,Shu-Ren Zhang 대한화학회 2011 Bulletin of the Korean Chemical Society Vol.32 No.12

The chalcopyrite-type CuInSe_2 is a remarkable material for thin film solar cells owing to its electronic structure and optical response. Single-phase sphere-like CuInSe_2 nanocrystallite particles were prepared by a facile noninjection method with triethanolamine as the complexing agent and the solvent simultaneously. The period of the reaction was the key to form single-phase CuInSe_2 nanocrystals at 240 ^oC. TEM, XRD, XPS, EDX investigations were performed to characterize the morphology and the detailed structure of as-synthesized CuInSe_2 nanocrystals. All of the analysis results proved that the synthesized nanocrystals were pure phase and close to the stoichiometric ratio rather than a simple mixture. The band gap of the obtained CuInSe_2nanocrystals was 1.03 ± 0.03 eV.

Empirical decomposition method for modeless component and its application to VIV analysis

Chen, Zheng-Shou,Park, Yeon-Seok,Wang, Li-ping,Kim, Wu-Joan,Sun, Meng,Li, Qiang The Society of Naval Architects of Korea 2015 International Journal of Naval Architecture and Oc Vol.7 No.2

Aiming at accurately distinguishing modeless component and natural vibration mode terms from data series of nonlinear and non-stationary processes, such as Vortex-Induced Vibration (VIV), a new empirical mode decomposition method has been developed in this paper. The key innovation related to this technique concerns the method to decompose modeless component from non-stationary process, characterized by a predetermined 'maximum intrinsic time window' and cubic spline. The introduction of conceptual modeless component eliminates the requirement of using spurious harmonics to represent nonlinear and non-stationary signals and then makes subsequent modal identification more accurate and meaningful. It neither slacks the vibration power of natural modes nor aggrandizes spurious energy of modeless component. The scale of the maximum intrinsic time window has been well designed, avoiding energy aliasing in data processing. Finally, it has been applied to analyze data series of vortex-induced vibration processes. Taking advantage of this newly introduced empirical decomposition method and mode identification technique, the vibration analysis about vortex-induced vibration becomes more meaningful.