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QingLing Zhai,KaiXin Wang,Defu Zhang,Jinbo Chen,XiaoMeng Dong,Yonghui Pan 대한통증학회 2023 The Korean Journal of Pain Vol.36 No.3
Background: Perampanel, a highly selective glutamate AMPA receptor antagonist, is widely used to treat epilepsy. Since the existence of common pathophysiological features between epilepsy and migraine, the aim of this study was to investigate whether perampanel could exert an antimigraine effect. Methods: Nitroglycerin (NTG) was used to induce a migraine model in rats, and the model animals were pretreatment with 50 μg/kg and 100 μg/kg perampanel. The expression of pituitary adenylate-cyclase-activating polypeptide (PACAP) was quantified by western blot and quantitative real-time PCR in the trigeminal ganglion, and rat-specific enzyme-linked immunosorbent assay in serum. Western blot was also conducted to explore the effects of perampanel treatment on the phospholipase C (PLC)/protein kinase C (PKC) and protein kinase A (PKA)/ cAMP-responsive-element-binding protein (CREB) signaling pathways. Moreover, the cAMP/PKA/CREB-dependent mechanism was evaluated via in vitro stimulation of hippocampal neurons. The cells were treated with perampanel, antagonists and agonists for 24 hours and cell lysates were prepared for western blot analysis. Results: Perampanel treatment notably increased the mechanical withdrawal threshold and decreased head grooming and light-aversive behaviors in NTG-treated rats. It also decreased PACAP expression and affected cAMP/ PKA/CREB signaling pathway. However, PLC/PKC signaling pathway may not be involved in this treatment. In in vitro studies, perampanel notably decreased PACAP expression by inhibiting cAMP/PKA/CREB signaling pathway. Conclusions: This study shows that perampanel inhibits the migraine-like pain response and that this beneficial effect might be attributable to regulation of the cAMP/PKA/CREB signaling pathway.
Molecular detection and genetic diversity of bovine papillomavirus in dairy cows in Xinjiang, China
Qingling Meng,Chengcheng Ning,Lixia Wang,Yan Ren,Jie Li,Chencheng Xiao,Yanfang Li,Zhiyuan Li,Zhihao He,Xuepeng Cai,Jun Qiao 대한수의학회 2021 Journal of Veterinary Science Vol.22 No.4
Background: Bovine papillomatosis is a type of proliferative tumor disease of skin and mucosae caused by bovine papillomavirus (BPV). As a transboundary and emerging disease in cattle, it poses a potential threat to the dairy industry. Objectives: The aim of this study is to detect and clarify the genetic diversity of BPV circulating in dairy cows in Xinjiang, China. Methods: 122 papilloma skin lesions from 8 intensive dairy farms located in different regions of Xinjiang, China were detected by polymerase chain reaction. The genetic evolution relationships of various types of BPVs were analyzed by examining this phylogenetic tree. Results: Ten genotypes of BPV (BPV1, BPV2, BPV3, BPV6, BPV7, BPV8, BPV10, BPV11, BPV13, and BPV14) were detected and identified in dairy cows. These were the first reported detections of BPV13 and BPV14 in Xinjiang, Mixed infections were detected, and there were geographical differences in the distribution of the BPV genotypes. Notably, the BPV infection rate among young cattle (< 1-year-old) developed from the same supply of frozen sperm was higher than that of the other young cows naturally raised under the same environmental conditions. Conclusions: Genotyping based on the L1 gene of BPV showed that BPVs circulating in Xinjiang China displayed substantial genetic diversity. This study provided valuable data at the molecular epidemiology level, which is conducive to developing deep insights into the genetic diversity and pathogenic characteristics of BPVs in dairy cows.
Qingling Zhang,Hui Lv,Junchao Ren 제어·로봇·시스템학회 2016 International Journal of Control, Automation, and Vol.14 No.4
This paper deals with the problem of robust normalization and delay-dependent H∞ control for a classof singular Markovian jump systems with norm-bounded parameter uncertainties and time delay. A new impulsiveand proportional-derivative control strategy with memory is presented, which results in a novel class of hybridimpulsive systems. Sufficient conditions are developed to guarantee that the resultant closed-loop system is notonly robust normal and stochastically stable, but also satisfies a prescribed H¥ performance level for all delays nolarger than a given upper bound. In addition, the explicit expression of the desired impulsive control gains is alsogiven together with the design approach. Finally, two numerical examples are provided to illustrate the effectivenessof the proposed methods.
Fang, Qingling,Kim, Dong-Pyo,Li, Xiaodong,Yoon, Tae-Ho,Li, Yihe Royal Society of Chemistry 2011 Lab on a chip Vol.11 No.16
<P>Highly effective mixing in microchannels is important for most chemical reactions conducted in microfluidic chips. To obtain a rigid and chemically resistant micromixer system at low cost, we fabricated a Y-shaped microchannel with built-in mixer structures by static liquid photolithography (SLP) from methacrylated polyvinylsilazane (MPVSZ) as an inorganic polymer photoresist which was then converted to a silicate phase by hydrolysis in vaporized ammonia atmosphere at 80 °C. The microchannel incorporating herringbone mixer structures was bonded with a matching polydimethylsiloxane (PDMS) open channel which was pre-coated by perhydropolysilazane (PHPS)-based mixture, and finally treated by additional hydrolysis at room temperature to convert the PHPS layer to a silica phase. Finally, the chemical resistance of the microfluidic system with embedded micromixer was confirmed with various solvents, and the excellent mixing performance in a short mixing length of 2.3 cm was demonstrated by injecting two different colored fluids into the microchannel.</P> <P>Graphic Abstract</P><P>A photocurable inorganic polymer as a silicate precursor was used to fabricate a passive micromixer system by static liquid photolithography (SLP). <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1lc20118b'> </P>
Direct pattern transfer using an inorganic polymer-derived silicate etch mask
Fang, Qingling,Li, Xiaodong,Tuan, Anh Pham,Perumal, Jayakumar,Kim, Dong-Pyo Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.12
<P>Advancements in patterning materials combined with facile fabrication techniques have been critical for the development of the semiconductor industry. In order to develop a new silicate etch mask for direct pattern transfer onto silicon <I>via</I> etching, methacrylated polyvinylsilazane, an inorganic polymer photoresist acting as a patternable silicate precursor, was used to fabricate micron-scale patterns over a large area upon 6 inch silicon wafers by conventional photolithography. The photolithographic patterns obtained were hydrolyzed to convert the polymer phase into the silicate phase after post-heat treatment at 150 °C. The silicate patterns were transferred as an etch mask onto silicon wafers <I>via</I> dry plasma etching process using various ratios of an SF<SUB>6</SUB>/Cl<SUB>2</SUB>/Ar mixture as etchant. The silicate patterns revealed an excellent etching selectivity of 16 for silicon, which was 8–16 times higher than the polymer phase.</P> <P>Graphic Abstract</P><P>Photocurable inorganic polymer-derived silicate was directly patterned and used as silicon etch mask with high selectivity. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c0jm03869e'> </P>
왕경령(Qingling Wang),김관석(Kwan-Suk Kim) 충북대학교 동물생명과학연구소 2010 동물생명과학연구 Vol.3 No.-
소 염색체 2번 에 위치하는 GDF8유전자는 근육발달의 중요한 역할을 하는 성장관련 유전자이다. 소 GDF 유전자내에서 존재하는 DNA 변이는 근육함량의 증가요인으로 연구되었다. DERA 유전자는 알돌축합반응에 관여하는 효소인데, 한우 염색체 5번의 성장 및 육량관련 QTL좌위에 위치하는 것으로 조사되었다. 본 연구에서는 GDF8과 DERA유전자의 염기서열을 분석하여, 단백질 수준에서의 기능에 영향을 줄 수 있는 DNA변이의 유무를 조사하기 위해서 수행하였다. 3개의 엑손으로 이루어진 GDF8유전자와 DERA 유전자의 4개의 엑손에 대한 PCR 및 염기서열 분석을 수행하였다. 예비적 염기서열 분석결과 한우 GDF2유전자의 엑손 2번에서 두 개의 SNP (NT414 C/T; NT574 T/C)가 발굴되었으나. 아미노산 조성에는 영향을 미치지 않았다. 그리고 DERA 유전자의 조사한 엑손영역내에서는 DNA 변이가 발견되지 않았다. The GDF8 act as a negative regulatory protein of muscle growth, which has been found several SNPs associated with increased lean muscle mass. DERA gene is known for acting as an enzyme which can catalyzes a sequential aldol reaction. The objectives of this study were to investigate single nucleotide polymorphisms (SNPs) of the two genes in Korean cattle (Hanwoo) which includes three exons of GDF8 (in the coding sequence) and four exons of DERA (3, 5, 6 and 8). All exons of the two genes were amplified by polymerase chain reaction and se-quenced. The preliminary results revealed two synonymous changes in the exon 2 of GDF8; polymorphisms are in NT414 (C/T) and NT574 (T/C). NT416 (G/A) has been previously reported in the Hanwoo breed. However, no DNA variation has been found in the coding region of the DERA gene in the Hanwoo breed.
Visual detection of copper ions based on azide- and alkyne-functionalized polydiacetylene vesicles
Xu, Qingling,Lee, Kyung Mi,Wang, Fang,Yoon, Juyoung Royal Society of Chemistry 2011 Journal of materials chemistry Vol.21 No.39
<P>A new method for visual detection of Cu<SUP>2+</SUP> based on azide- and alkyne-functionalized polydiacetylene (PDA-aa) vesicles has been developed. In the presence of ascorbic acid, Cu<SUP>2+</SUP> can be reduced to Cu<SUP>+</SUP> and catalyze the click reaction between the two functional groups. After incubation with Cu<SUP>2+</SUP> and ascorbic acid, a PDA-aa solution changed its color from blue to red, which was attributed to conformational transition of the conjugated backbone. Other metal ions were explored but only induced negligible color changes. This method provides a naked eye detection of Cu<SUP>2+</SUP> in water without the aid of complex instruments.</P> <P>Graphic Abstract</P><P>A new Cu<SUP>2+</SUP> sensor based on polydiacetylene vesicles with imidazolium linked azide and alkyne groups was developed using click chemistry. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=c1jm11660f'> </P>