한방의료체험이 한방의료관광 브랜드자산에 미치는 영향

양청청(Qing Qing Liang),이대휘(Dae Hui Lee) 한국관광연구학회 2014 관광연구저널 Vol.28 No.7

The purpose of this study is to examine the effects of oriental medical experiences on brand equity of oriental medical tourism. Chinese medical tourists who visited the World Traditional Medicine Expo in Sancheong were examined in this study. The results indicate that there were four factors (i.e., entertainment, education, escapism, esthetics) in oriental medical experiences. First, entertainment of oriental medical experiences significantly influenced brand association, brand awareness, and perceived quality. Second, education of oriental medical experiences significantly influenced brand awareness and perceived quality. Third, escapism and esthetics didn`t significantly influence any brand equity of oriental medical tourism. Forth, brand association and brand awareness of oriental medical tourism significantly influenced brand loyalty. This study will be useful in enhancing the competitiveness of oriental medical tourism and enticing foreign medical tourists who are interested in oriental medical tourism to visit oriental medical events in Korea.

Radiosensitivity Enhancement by Arsenic Trioxide in Conjunction with Hyperthermia in the EC-1 Esophageal Carcinoma Cell Line

Cui, Yan-Hui,Liang, Hai-Jun,Zhang, Qing-Qin,Li, Si-Qing,Li, Xiao-Rui,Huo, Xiao-Qing,Yang, Qing-Hui,Li, Wei-Wei,Gu, Jian-Fa,Hua, Qin-Liang,Lu, Ping,Miao, Zhan-Hui Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.4

Objective: To explore the effect on radiosensitivity of arsenic trioxide ($As_20_3$) in conjunction with hyperthermia on the esophageal carcinoma EC-1 cell line. Method: Inhibition of EC-1 cell proliferation at different concentrations of $As_20_3$ was assessed using the methyl thiazolyl blue colorimetric method (MTT method), with calculation of $IC_{50}$ value and choice of 20% of the $IC_{50}$ as the experimental drug concentration. Blank control, $As_20_3$, hyperthermia, radiotherapy group, $As_20_3$ + hyperthermia, $As_20_3$ + radiotherapy, hyperthermia + radiotherapy and $As_20_3$ + hyperthermia + radiotherapy groups were established, and the cell survival fraction (SF) was calculated from flat panel colony forming analysis, and fitted by the 'multitarget click mathematical model'. Flow cytometry (FCM) was used to detect changes in cell apoptosis and the cell cycle. Results: $As_20_3$ exerted inhibitory effects on proliferation of esophageal carcinoma EC-1 cells, with an $IC_{50}$ of 18.7 ${\mu}mol/L$. After joint therapy of $As_20_3$ + hyperthermia + radiotherapy, the results of FCM showed that cells could be arrested in the $G_2$/M phase, and as the ratio of cells in $G_0/G_1$ and S phases decreased, cell death became more pronounced. Conclusion: $As_20_3$ and hyperthermia exert radiosensitivity effects on esophageal carcinoma EC-1 cells, with synergy in combination. Mechanistically, $As_20_3$ and hyperthermia mainly influence the cell cycle distribution of EC-1 esophageal carcinoma cells, decreasing the repair of sublethal damage and inducing apoptosis, thereby enhancing the killing effects of radioactive rays.

An Estimation Method of Consolidation and Settlement of Soil Mass in Dredger Fill Area Based on SBAS-InSAR

( Qing-bo Yu ),( Qing Wang ),( Xue-xin Yan ),( Tian-liang Yang ),( Meng Yao ),( Kai Zhou ),( Xin-lei Huang ),( Jian-ping Chen ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

The land subsidence poses a nonnegligible threat to production and life, especially in dredger fill area. The essence of land subsidence is soil consolidation. Better evaluation of the degree of soil consolidation presents significant progress for predicting land subsidence. In this study, SBAS-InSAR was conducted to Chenjia town, the typical dredger fill area in Chongming East Shoal, Shanghai. SAR data and precise Orbit data were collected from the ESA satellite Sentinel-1 in recent 2 years with approximately one month intervals. the relationship curve of cumulative subsidence with time is obtained after processing. On this basis, the final settlement and soil consolidation can be estimated by applying the method proposed by Professor Huang Wenxi of China. The results show that: The subsidence rate is faster than 20 mm/a; The estimated final settlement is 26.6-52.9 mm, and the corresponding consolidation degree is 54.6 % on average; Soil mass has not yet been fully consolidated and it still takes a long time to achieve stability. In conclusion, the method proposed in this study can provide a clearer understanding of the consolidation degree, and a new reference for the evaluation and prevention of land subsidence. Future research will focus on longer monitoring time to enrich and improve the method. Acknowledgements This work was financially supported by National Nature Science Foundation of China (Grant No: 41820104001, 41430642 and 41627801) and the Key Laboratory of Land Subsidence Monitoring and Prevention, Ministry of Land and Resources (Grant No. KLLSMP201801).

Mechanical Effect of Pre-consolidation Pressure of Structural Behavior Soil

( Qing Wang ),( Qing-bo Yu ),( Tian-liang Yang ),( Xin-lei Huang ),( Xue-xin Yan ) 대한지질공학회 2019 대한지질공학회 학술발표회논문집 Vol.2019 No.2

Structural property of soil is a new concept studied in science and study of this issue is a new problem, which involves soil mechanical effect, mechanical variability, and engineering design. Soil such as saline soil, red soil, loess, Xiashu loess, soft soil, or solidified dredger fill, which had been studied in previous research by our research group, has high pre-consolidation pressure (Pc) and high over-consolidation ratio (OCR). Considering the needs of intensity determination in engineering design, soil structure force, structural strength and other issues of structural Soils were studied in this paper. By analyzing experimental data, affecting factors of pre-consolidation pressure (Pc) was discussed. A new cognition about pre-consolidation pressure (Pc), determination method of structural strength, and genetic mechanism of structural force were proposed in this paper. Soil mass structural behavior and the mechanical effect can be one of the important fields of soil structural quantitative research. Acknowledgements This work was financially supported by the Key Project for International (Regional) Cooperation and Communication by National Natural Science Foundation of China (Grant No. 41820104001), State Key Program of the National Natural Science Foundation of China (Grant No. 41430642), the Special Fund for Major Scientific Instruments of the National Natural Science Foundation of China (Grant No. 41627801), and the Key Laboratory of Land Subsidence Monitoring and Prevention, Ministry of Land and Resources (Grant No. KLLSMP201801).

MiR-454 Prompts Cell Proliferation of Human Colorectal Cancer Cells by Repressing CYLD Expression

Liang, Hong-Liang,Hu, Ai-Ping,Li, Sen-Lin,Xie, Jia-Ping,Ma, Qing-Zhu,Liu, Ji-Yong Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.6

Previous studies have shown that miR-454 plays an important role in a variety of biological processes in various human cancer cells. However, the underlying mechanisms of this microRNA in colorectal cancer (CRC) cells remain largely unknown. In the present study, we investigated the miR-454 role in CRC cell proliferation. We found that miR-454 expression is markedly upregulated in CRC tissues and CRC cells compared with the matched tumor adjacent tissues and the FHC normal colonic cell line. Ectopic expression of miR-454 promoted the proliferation and anchorage-independent growth of CRC cells, whereas inhibition of miR-454 reduced this effect. Bioinformatics analysis further revealed cylindromatosis (CYLD), a putative tumor suppressor as a potential target of miR-454. Data from luciferase reporter assays showed that miR-454 directly binds to the 3'-untranslated region (3'-UTR) of CYLD mRNA and repressed expression at both transcriptional and translational levels. In functional assays, CYLD-silenced in miR-454-in-transfected SW480 cells have positive effect to promote cell proliferation, suggesting that direct CYLD downregulation is required for miR-454-induced CRC cell proliferation. In sum, our data provide compelling evidence that miR-454 functions as an onco-miRNA, playing a crucial role in the promoting cell proliferation in CRC, and its oncogenic effect is mediated chiefly through direct suppression of CYLD expression.

Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc mesenteroides LM187

( Qing Zhang ),( Jie Wang ),( Qing Sun ),( Shu-ming Zhang ),( Xiang-yang Sun ),( Chan-yuan Li ),( Miao-xin Zheng ),( Wen-liang Xiang ),( Jie Tang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8

A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 10⁵ Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. ¹H and ¹³C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.

Effects of Electrostatic Field on Cross Compatibility and Embryo Rescue in Interspecific Crosses between Prunus avium L. and Prunus pseudoerasus L

Qing Liang,Xuesen Chen,Wen Liu,Lijie Zhang 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-

Rheological Behaviors and Microstructure of Oviductus Ranae Hydrogels

Qing Liang,Shouqin Zhang,Jinsong Zhang 한국식품과학회 2012 Food Science and Biotechnology Vol.21 No.2

The rheological properties of Oviductus Ranae (OR) hydrogels were systematically investigated with shear viscosity, dynamic oscillation, and creep-recovery measurements. The viscosity curves displayed phenomena of shear thinning with increase of shear rate. The flow behaviors of the hydrogels were described using 2representative rheological models. The lesser water was absorbed by OR, the higher viscosity and greater extent of thixotropy it presents. Dynamic viscoelasticity measurements indicated that the samples exhibit viscoelastic properties as physical gels. Creep curves also revealed that the hydrogels behave as viscoelastic solids. Damped oscillations were observed in the initial stage of creep tests (0-1 s). The viscoelastic moduli obtained from dynamic oscillations and those from damped oscillations agreed with each other. The strong dependence of rheological behaviour on network of OR hydrogels had been confirmed by electron microscopy.

Splenectomy improves liver fibrosis via tumor necrosis factor superfamily 14 (LIGHT) through the JNK/TGF-β1 signaling pathway

Liang Qing-shan,Xie Jian-Gang,Yu ChaoPing,Feng ZhuSheng,Ma JingChang,Zhang Yuan,Wang Dong,Lu JianGuo,Zhuang Ran,Yin Jikai 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Splenectomy has been reported to improve liver fibrosis in patients with cirrhosis and hypersplenism. However, the mechanisms remain unclear. Tumor necrosis factor superfamily 14 (TNFSF14; also known as LIGHT) is highly expressed in the context of fibrosis and promotes disease progression in patients with fibrotic diseases such as pulmonary and skin fibrosis. Here, we determined whether splenectomy controls the production of LIGHT to improve liver fibrosis. Splenectomy reduced serum LIGHT levels in cirrhotic patients with hypersplenism and a ConA-induced liver fibrosis mouse model. Blocking LIGHT resulted in the downregulation of TGF-β1 in RAW264.7 cells. LIGHT treatment of RAW264.7 and JS1 cells in coculture regulated transforming growth factor-β1 (TGF-β1) expression through the activation of JNK signaling. Small interfering RNA-mediated silencing of lymphotoxin β receptor (LTβR) in macrophages resulted in pronounced decreases in the levels of fibrosis and αSMA in JS1 cells. These results indicated that LIGHT bound to LTβR and drove liver fibrosis in vitro. Blocking TGF-β1 abolished the effect of LIGHT in vitro. Furthermore, the administration of recombinant murine LIGHT protein-induced liver fibrosis with splenectomy, while blocking LIGHT without splenectomy improved liver fibrosis in vivo, revealing that the decrease in fibrosis following splenectomy was directly related to reduced levels of LIGHT. Thus, high levels of LIGHT derived from the spleen and hepatic macrophages activate JNK signaling and lead to increased TGF-β1 production in hepatic macrophages. Splenectomy attenuates liver fibrosis by decreasing the expression of LIGHT.

Reduction of fatty acid flux at low temperature led to enhancement of β-carotene biosynthesis in recombinant Saccharomyces cerevisiae

Liang Sun,Fei Shang,Chang-Qing Duan,Guo-liang Yan 한국화학공학회 2015 Korean Journal of Chemical Engineering Vol.32 No.7

Transferring the recombinant S. cerevisiae T73-63 from 30 oC to 4 oC resulted in 41.4% increment of β-carotene concentration (3.96mg/g dry cell weight) relative to that of 30 oC, which was accompanied with the accumulation of fatty acid and ergosterol. The comparisons of the transcriptional levels of mevalonate pathway genes indicated that the expressions of HMG1, ERG9, ERG19, ERG20 and IDI1 at 4 oC were all higher than those of 30 oC, respectively. This suggested that increased transcriptions of mevalonate pathway genes contribute to the improvement of β-carotene production at low temperature. We also found that supplementation of 30mg/L triclosan, an inhibitor of fatty acid synthesis, led to further 28.3% enhancement of β-carotene concentration (4.94mg/g DCW), which was 18.8% higher than that of 30 oC with the same concentration of triclosan. The higher expressional levels of HMG, ERG19 and ERG20 and the simultaneous increment of ergosterol content (17.8%) suggested that more carbon source was transferred from fatty acid synthesis to mevalonate pathway under the circumstance of appropriately blocking fatty acid synthesis at low temperature (4 oC), which resulted in a higher increment of β-carotene production compared to that of 30 oC. The results of this study collectively suggest that the combination of reducing temperature and adding fatty acid synthesis inhibitors is a potential approach to improve the production of desirable isoprenoid compounds such as carotenoids.