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Enxian Yuan,Chan Wu,Guozhu Liu,Guozhu Liu,Li Wang 한국공업화학회 2018 Journal of Industrial and Engineering Chemistry Vol.66 No.-
A series of SBA-15 mesoporous silicas with pore sizes ranging between 7 and 26 nm were synthesized and modified by incorporating Al or Mg and functionalization with n-dodecyltrimethoxysilane. Pd/SBA-15 catalysts were prepared and the effects of the pore structure, Al/Mg modifiers and Pd particles distribution on 2-ethyl-anthraquinone hydrogenation have been studied. The catalytic performance improves with enlarging SBA-15 pore size and reaches a plateau at 22.3 nm. The incorporation of Mg into SBA-15 increases the catalytic performance of catalysts. But, the incorporation of Al brings about opposite results. The functionalization of SBA-15 with n-dodecyltrimethoxysilane further enhances the catalytic performance.
Ziqian Li,Kaying Chan,Yifei Qi,Linlin Lu,Fen Ning,Mengling Wu,Haifang Wang,Yuan Wang,Shaohui Cai,Jun Du 대한암학회 2018 Cancer Research and Treatment Vol.50 No.3
Purpose Cancer-associated fibroblasts (CAFs) activated by cancer cells has a central role in development and malignant biological behavior in colorectal cancer (CRC). Adult fibroblasts do not express Snail, but Snail-positive fibroblasts are discovered in the stroma of malignant CRC and reported to be the key role to chemoresistance. However, the reciprocal effect of CAFs expressed Snail to chemoresistance on CRC cells and the underlying molecular mechanisms are not fully characterized. Materials and Methods Snail-overexpressed 3T3 stable cell lines were generated by lipidosome and CT26 mixed with 3T3-Snail subcutaneous transplanted CRC models were established by subcutaneous injection. Cell Counting Kit-8, flow cytometry and western blotting assays were performed, and immunohistochemistry staining was studied. The cytokines participated in chemoresistance was validated with reverse transcriptase-polymerase chain reaction and heatmap. Results Snail-expression fibroblasts are discovered in human and mouse spontaneous CRCs. Overexpression of Snail induces 3T3 fibroblasts transdifferentiation to CAFs. CT26 co-cultured with 3T3-Snail resisted the impairment from 5-fluorouracil and paclitaxel in vitro. The subcutaneous transplanted tumor models included 3T3-Snail cells develop without restrictions even after treating with 5-fluorouracil or paclitaxel. Moreover, these chemoresistant processes may be mediated by CCL1 secreted by Snail-expression fibroblasts via transforming growth factor /nuclear factor-B signaling pathways. Conclusion Taken together, Snail-expressing 3T3 fibroblasts display CAFs properties that support 5-fluorouracil and paclitaxel chemoresistance in CRC via participation of CCL1 and suggest that inhibition of the Snail-expression fibroblasts in tumor may be a useful strategy to limit chemoresistance.
Mobility-Sensitive Multicast Protocol in NEMO
Long-Sheng Li,Hung-I Chi,Kai-Chung Xie,Din-Yuan Chan 한국인터넷정보학회 2022 KSII Transactions on Internet and Information Syst Vol.16 No.6
In view of the past, the mobility of the multicast source in the mobility networks is seldom discussed in the traditional multicast protocols. It is a heavy cost for the traditional multicast protocols to reconstruct the multicast tree in the Network Mobility (NEMO) environment. This article proposes an alternative multicast protocol, referred to as Mobility-Sensitive Multicast protocol (MSM), for the NEMO environment. The MSM can be considered as an alternative version of the Multicast Listener Discovery (MLD) protocol to maintain the multicast tree in the NEMO. There are two obvious contributions for the MSM. Reconstruct mechanism could rebuild the multicast tree for the mobility of the multicast source. Multi-group suppression mechanism reduce the multicast tree maintaining cost for the mobility of the multicast members. Through the performance evaluations and analyses, the MSM has less cost to maintain the multicast tree than the traditional multicast protocols, especially for a large numbers of multicast groups. Moreover, the MSM allows the mobility of the multicast source to reconstruct the multicast tree easily.
Kojic Acid Protects C57BL/6 Mice from Gamma-irradiation Induced Damage
Wang, Kai,Liu, Chao,Di, Chan-Juan,Ma, Cong,Han, Chun-Guang,Yuan, Mei-Ru,Li, Peng-Fei,Li, Lu,Liu, Yong-Xue Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1
The radioprotective effects of a single administration of kojic acid (KA) against ionizing radiation were evaluated via assessment of 30-day survival and alterations of peripheral blood parameters of adult C57BL/6 male mice. The 30-day survival rate of mice pretreated with KA (75 or 300 mg/kg body weight, KA75 or KA300) subcutaneously 27 h prior to a lethal dose (8 Gy, 153.52 cGy/min) of gamma irradiation was higher than that of mice irradiated alone (40% or 60% vs 0%). It was observed that the white blood cell (WBC) count/the red blood cell (RBC) count, haemoglobin content, haematocrit and platelet count of mice with or without KA pretreatment as exposed to a sub-lethal dose (4 Gy, 148.14 cGy/min) of gamma irradiation decreased maximally at day 4/day 8 post-irradiation. Although the initial WBC values were low in KA300 or WR-2721 (amifostine) groups, they significantly recovered to normal at day 19, whereas in the control group they did not. The results from the cytotoxicity and cell viability assays demonstrated that KA could highly protect Chinese hamster ovary (CHO) cells against ionizing radiation with low toxicity. In summary, KA provides marked radioprotective effects both in vivo and in vitro.
Tang, Shu-Kun,Zhi, Xiao-Yang,Wang, Yun,Wu, Jin-Yuan,Lee, Jae-Chan,Kim, Chang-Jin,Lou, Kai,Xu, Li-Hua,Li, Wen-Jun Microbiology Society 2010 International journal of systematic and evolutiona Vol.60 No.9
<P>A Gram-staining-positive, facultatively anaerobic, non-motile and moderately halophilic actinobacterium, designated YIM 93306<SUP>T</SUP>, was isolated from a salt lake in Xinjiang province, north-west China, and subjected to a polyphasic taxonomic study. Strain YIM 93306<SUP>T</SUP> grew in the presence of 2-16 % (w/v) NaCl and did not grow without NaCl. The peptidoglycan type was A4<I>α</I> with an l-Lys-l-Glu interpeptide bridge. The whole-cell sugars were glucosamine, arabinose, mannose and two unknown sugars. The predominant menaquinone was MK-8(H4). The major fatty acids were iso-C15 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polar lipids comprised diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, one unknown phosphoglycolipid and one unknown phospholipid. The DNA G+C content was 68.3 mol%. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain YIM 93306<SUP>T</SUP> fell within the radius of the suborder <I>Micrococcineae</I>. Its closest phylogenetic neighbour was the type strain of <I>Ruania albidiflava</I> (AS 4.3142<SUP>T</SUP>; 96.2 % 16S rRNA gene sequence similarity), the sole recognized species of the genus <I>Ruania</I>. Sequence similarities between strain YIM 93306<SUP>T</SUP> and members of other genera of the suborder <I>Micrococcineae</I> were <95.2 %. On the basis of phylogenetic analysis, phenotypic characteristics and chemotaxonomic differences, a novel genus and species, <I>Haloactinobacterium album</I> gen. nov., sp. nov., is proposed. The type strain of the species is YIM 93306<SUP>T</SUP> (=DSM 21368<SUP>T</SUP> =KCTC 19413<SUP>T</SUP> =CCTCC AB 208069<SUP>T</SUP>). Based on phylogenetic characteristics and 16S rRNA gene signature nucleotide patterns, the genera <I>Ruania</I> and <I>Haloactinobacterium</I> gen. nov. are proposed to belong to a novel family, <I>Ruaniaceae</I> fam. nov.</P>
( Qing Zhang ),( Jie Wang ),( Qing Sun ),( Shu-ming Zhang ),( Xiang-yang Sun ),( Chan-yuan Li ),( Miao-xin Zheng ),( Wen-liang Xiang ),( Jie Tang ) 한국미생물 · 생명공학회 2021 Journal of microbiology and biotechnology Vol.31 No.8
A released exopolysaccharide (rEPS)-producing strain (LM187) with good acid resistance, bile salt resistance, and cholesterol-lowering properties was isolated from Sichuan paocai and identified as Leuconostoc mesenteroides subsp. mesenteroides. The purified rEPS, designated as rEPS414, had a uniform molecular weight of 7.757 × 10<sup>5</sup> Da. Analysis of the monosaccharide composition revealed that the molecule was mainly composed of glucose. The Fourier transform-infrared spectrum showed that rEPS414 contained both α-type and β-type glycosidic bonds. <sup>1</sup>H and <sup>13</sup>C nuclear magnetic resonance spectra analysis showed that the purified rEPS contained arabinose, galactose, and rhamnose, but less uronic acid. Scanning electron microscopy demonstrated that the exopolysaccharide displayed a large number of scattered, fluffy, porous cellular network flake structures. In addition, rEPS414 exhibited strong in vitro antioxidant activity. These results showed that strain LM187 and its rEPS are promising probiotics with broad prospects in industry.