Distinct mutations in MLH1 and MSH2 genes in Hereditary Non-polyposis Colorectal Cancer (HNPCC) families from China

( Wen Qian Wei ),( Fang Qi Liu ),( Lei Liu ),( Zuo Feng Li ),( Xiao Yan Zhang ),( Fan Jiang ),( Qu Shi ),( Xiao Yan Zhou ),( Wei Qi Sheng ),( San Jun Cai ),( Xuan Li ),( Ye Xu ),( Peng Nan ) 생화학분자생물학회(구 한국생화학분자생물학회) 2011 BMB Reports Vol.44 No.5

Hereditary non-polyposis Colorectal Cancer (HNPCC) is an autosomal dominant inheritance syndrome. HNPCC is the most common hereditary variant of colorectal cancer (CRC), which accounts for 2-5% CRCs, mainly due to hMLH1 and hMSH2 mutations that impair DNA repair functions. Our study aimed to identify the patterns of hMSH2 and hMLH1 mutations in Chinese HNPCC patients. Ninety-eight unrelated families from China meeting Amsterdam or Bethesda criteria were included in our study. Germline mutations in MLH1 and MSH2 genes, located in the exons and the splice-site junctions, were screened in the 98 probands by direct sequencing. Eleven mutations were found in ten patients (11%), with six in MLH1 (54.5%) and five in MSH2 (45.5%) genes. One patient had mutations in both MLH1 and MSH2 genes. Three novel mutations in MLH1 gene (c.157_160delGAGG, c.2157dupT and c.-64G>T) were found for the first time, and one suspected hotspot in MSH2 (c.1168C>T) was revealed. [BMB reports 2011; 44(5): 317-322]

Cathodic reduction characteristics of 2-chloro-4-nitrophenol in microbial electrolysis cell

Qianli Yu,Wei Xiong,Donggen Huang,Cui Luo,Qiang Yang,Tao Guo,Qi Wei 대한환경공학회 2020 Environmental Engineering Research Vol.25 No.6

Microbial electrolysis cell (MEC) has been constructed to study the degradation characters of 2-chloro-4-nitrophenol (2C4NP) in waste water. The effects of applied voltage, initial concentration of substrate and co-matrix species on the reduction and degradation of 2C4NP were studied. Qualitative and quantitative analysis of 2C4NP residues and degradation intermediate by using UV-Vis, HPLC, HPLC/MS/MS, IC and other analytical testing techniques. The degradation mechanism of 2C4NP in MEC cathode was proposed. The results showed that electron and electroactive microorganisms would produce coupling effect and accelerate the degradation of 2C4NP under adding 0.5 V DC; Under the condition of satisfying the C/N ratio of electroactive anaerobic microorganism, the addition of organic substances such as glucose and sodium acetate which were easily degraded by microorganisms would hinder the degradation of 2C4NP in the cathode compartment. 2C4NP can be effectively degraded by adding appropriate amount of glucose as carbon source with the low C/N. 2C4NP undergoes reduction, dechlorination, denitrification and assimilation in the cathode compartment to form 2-chloro-4-aminophenol, 4-aminophenol, 2-chlorophenol, 2-chloro-4-hydroxyphenol, nitrophenol, hydroquinone, 4-hydroxyhexadienoic acid semialdehyde, valeric acid, oxalic acid and many other intermediate products. According to the degradation intermediates, the degradation mechanism of 2C4NP in the cathode compartment was presumed.

Prevalence and Genetic Characterization of Toxoplasma gondii in Pet Dogs in Central China

Wei-Feng Qian,Wen-Chao Yan,Tian-Qi Wang,Kai Zhai,Li-Fang Han,Chao-Chao Lv 대한기생충학열대의학회 2015 The Korean Journal of Parasitology Vol.53 No.1

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3’+5’) SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29- 2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.

Experimental Study on the Preparation of MA@PS@Fe3O4 Phase Change Microcapsules to Inhibit the Development of Electric Branches in Epoxy Resin Cured Compounds

Liu Qian,Du Bin,Qi Wei,Mai Yuxiang,Zhao Yushun,Chen Nanqing 대한금속·재료학회 2022 ELECTRONIC MATERIALS LETTERS Vol.18 No.5

Development of electric branches caused by partial discharge leads to degradation in the performance of epoxy resin insulation materials, which seriously threatens the safe and stable operation of power equipment. In this study, n-tetradecanol (MA)@polystyrene microsphere (PS)@Fe3O4 core–shell phase change microcapsules were designed and prepared. Doping 0.1 wt% phase change microcapsular material into the epoxy resin cured compound inhibited the development of electric branches. SEM and EDS tests showed that the phase-change microcapsules had monodisperse spherical core–shell structures with an MA encapsulation rate of 24.73% and excellent phase-change thermal storage capacity. Electric field simulations revealed that Fe3O4 nanoparticles in the microcapsule shell enhanced the local field strength of the cured epoxy resin and induced the development of electric branches toward the interior of the microcapsule. Moreover, doping of microcapsules into the epoxy resin significantly slowed the rate of temperature rise and thus inhibited further development of electric branches in epoxy resin cured products. In comparison with the epoxy resin cured without microcapsules, it was found that the longitudinal and transverse lengths of electric branches were reduced by 56.6% and 69.1%, respectively, in the epoxy resin cured with 0.1 wt% MA@PS@Fe3O4 microcapsules, and the electric branch initiation field strength was increased from 0.57 to 0.68 kV/mm. This indicated that MA@PS@Fe3O4 microcapsules significantly improved the electrical branch resistance of epoxy resin cured products, and this provides a new approach for extensive applications of epoxy resin insulation materials and safe and stable operation of power equipment.

Three P5CS genes including a novel one from Lilium regale play distinct roles in osmotic, drought and salt stress tolerance

Chi Wei,Qi Cui,Xi-Qing Zhang,Yu-Qian Zhao,Gui-Xia Jia 한국식물학회 2016 Journal of Plant Biology Vol.59 No.5

Proline accumulations in abiotically stressed plants is generally considered to benefit their stress tolerance. The Δ1-Pyrroline-5-carboxylate synthetase (P5CS) gene family, which encodes the rate-limiting enzyme in proline biosynthesis pathway, usually contains two duplicated genes in most plants. However, three P5CS genes including LrP5CS1, LrP5CS2 as well as a third one, LrP5CS3, were isolated from Lilium regale. LrP5CS3 is highly identical to LrP5CS1 in amino acid sequences, indicating they could come from a paralogous duplication. The phylogenetic tree suggested that the duplication of LrP5CS occurred independently after the divergence of Liliales and commelinoids. The expression of LrP5CS1 was strongly induced in leaves and roots both under drought and salinity, while that of LrP5CS3 was upregulated more moderately. LrP5CS2 stayed almost constitutive under stress. LrP5CS1 exhibited the highest activity after expressed in E. coli. Overexpression of LrP5CS genes conferred enhanced osmotic, drought and salt tolerance on transgenic Arabidopsis without negative effects in unstressed condition. Under salt stress, lines LrP5CS2 accumulated fewer proline than others, and lines LrP5CS1 grew better in root elongation. The roots of lines LrP5CS3 grew better than all others under unstressed condition and osmotic stress. Our study suggests that the three LrP5CS genes play distinct roles respectively in proline accumulation and abiotic stress tolerance.

SUV39H1 is a New Client Protein of Hsp90 Degradated by Chaetocin as a Novel C-Terminal Inhibitor of Hsp90

( Bin Lian ),( Qian Lin ),( Wei Tang ),( Xin Qi ),( Jing Li ) 한국응용약물학회 2021 Biomolecules & Therapeutics(구 응용약물학회지) Vol.29 No.1

Hsp90 is often overexpressed with activated form in cancer cells, and many key cellular proteins are dependent upon the Hsp90 machinery (these proteins are called “client protein”). Nowadays, more client proteins and more inhibitors of Hsp90 are being discovered. Chaetocin has been identified as an inhibitor of histone methyl transferase SUV39H1. Herein, we find that Chaetocin is an inhibitor of Hsp90 which binds to the C-terminal of Hsp90α. Chaetocin inhibited a variety of Hsp90 client proteins including AMl1-ETO and BCL-ABL, the mutant fusion-protein in the K562 and HL-60 cells. SUV39H1 mediates epigenetic events in the pathophysiology of hematopoietic disorders. We found that inhibition of Hsp90 by Chaetocin and 17-AAG had ability to induce degradation of SUV39H1 through proteasome pathway. In addition, SUV39H1 interacted with Hsp90 through co-chaperone HOP. These results suggest that SUV39H1 belongs to a client protein of Hsp90. Moreover, Chaetocin was able to induce cell differentiation in the two cells in the concentration range of Hsp90 inhibition. Altogether, our results demonstrate that SUV39H1 is a new client protein of Hsp90 degradated by Chaetocin as a novel C-terminal inhibitor of Hsp90. The study establishes a new relationship of Chaetocin and SUV39H1, and paves an avenue for exploring a new strategy to target SUV39H1 by inhibition of Hsp90 in leukemia.

Modeling and Performance Analysis of Wireless Sensor Network Systems Using Petri Nets

Song Chen,Qi-Wei Ge,Qian-Ming Shao,Qian Zhu 대한전자공학회 2008 ITC-CSCC :International Technical Conference on Ci Vol.2008 No.7

Wireless sensor network (WSN), composed by sensors, microprocessor and wireless communication interface, is an interesting field, and gains more and more attentions. The importance of sensor networks is highlighted by the number of recent funding initiatives, including the DARPA SENSIT program, military programs, and NSF Program Announcements. The wide application prospects make it developing rapidly in some fields such as health care, environment monitoring and military field. Petri nets are chosen for their ability to describe and study discrete event systems that characterized as being event systems that are characterized as being concurrent, asynchronous, parallel, nondeterministic, and stochastic. We construct ordinary and temporal Petri net models for wireless sensor network systems and use the model to solve the problem of location of nodes of wireless sensor network.

A Swimming Hydroponic System for Leafy Vegetable Production (ZAAS-SwimHydro)

Zhi-hao Xu,Wei-shon Shou,Cheng-hao Zhang,Wen-qi Dong,Jue-li Lei,Jian Cheng,Sheng-jun Zhou,Yu-qian Zhu,Dang-li Dai,Li-ping Cheng,Jian Xu,Jin-chun Fang,Sang Gyu Lee,Myeong-Cheoul Cho 한국원예학회 2008 한국원예학회 기타간행물 Vol.- No.-

Challenges and Advances in Materials and Fabrication Technologies of Small-Diameter Vascular Grafts

Mei‑Xian Li,Qian‑Qi Wei,Hui‑Lin Mo,Yu Ren,Wei Zhang,Huan‑Jun Lu,정윤기 한국생체재료학회 2023 생체재료학회지 Vol.27 No.00

The arterial occlusive disease is one of the leading causes of cardiovascular diseases, often requiring revascularization. Lack of suitable small-diameter vascular grafts (SDVGs), infection, thrombosis, and intimal hyperplasia associated with synthetic vascular grafts lead to a low success rate of SDVGs (< 6 mm) transplantation in the clinical treatment of cardiovascular diseases. The development of fabrication technology along with vascular tissue engineering and regenerative medicine technology allows biological tissue-engineered vascular grafts to become living grafts, which can integrate, remodel, and repair the host vessels as well as respond to the surrounding mechanical and biochemical stimuli. Hence, they potentially alleviate the shortage of existing vascular grafts. This paper evaluates the current advanced fabrication technologies for SDVGs, including electrospinning, molding, 3D printing, decellularization, and so on. Various characteristics of synthetic polymers and surface modification methods are also introduced. In addition, it also provides interdisciplinary insights into the future of small-diameter prostheses and discusses vital factors and perspectives for developing such prostheses in clinical applications. We propose that the performance of SDVGs can be improved by integrating various technologies in the near future.

RDW-SD and PCT Are Potential Prognostic Factors for In-hospital Death in Patients With Stevens-Johnson Syndrome/Toxic Epidermal Necrolysis

Zhang Lixia,Lan Yunping,Qi Bo,Shuai Ping,Hou Qinchuan,Liu Wei,Wang Qian 대한천식알레르기학회 2023 Allergy, Asthma & Immunology Research Vol.15 No.6

Purpose: Our study aimed to explore potential prognostic factors in Stevens-Johnson syndrome/toxic epidermal necrolysis (SJS/TEN) patients from easily accessible laboratory data and to investigate whether the combination of these indicators with a score for toxic epidermal necrolysis (SCORTEN) can improve the predictive value. Methods: Data from 85 SJS/TEN patients hospitalized from 2010 to 2021 were retrospectively analyzed. The primary outcome was in-hospital mortality. Univariate analysis was used to screen for laboratory indexes associated with death. Logistic regression was used to analyze significant risk factors for death. The differentiation and calibration of SCORTEN and modified score were assessed using receiver operating characteristic (ROC) curves and Hosmer-Lemeshow goodness-of-fit test. Net reclassification improvement (NRI) and integrated discrimination improvement (IDI) were used to evaluate the incremental prognostic value. Results: Among the 85 patients (37 males, 48 females) aged 14-88 years, the mortality rate was 11.8% (n = 10). SCORTEN had good discrimination and calibration to predict mortality in this cohort of patients (area under the ROC curve [AUC] of 0.874, 95% confidence interval [CI], 0.758-0.990; Hosmer-Lemeshow goodness-of-fit test P = 0.994). Red cell distribution width-standard deviation index (RDW-SD) > 47.9 fL and procalcitonin (PCT) > 0.67 ng/mL were significant risk factors for death. When adding the 2 factors to SCORTEN, AUC was 0.915 (95% CI, 0.833–0.997), but not statistically different compared to SCORTEN alone (P = 0.091). The NRI was 1.2 (95% CI, 0.672–1.728; P < 0.001) and the IDI was 0.09 (95% CI, 0.011–0.173; P = 0.026), still suggesting that the modified score had better discriminatory and predictive power than SCORTEN alone. The modified score also showed good calibration (Hosmer-Lemeshow goodness-of-fit test, P = 0.915). Conclusions: SCORTEN is a good predictor of mortality in SJS/TEN patients in southwest China. Combining RDW-SD > 47.9 fL and PCT > 0.67 ng/mL with SCORTEN may enhance the ability to predict prognosis.