Effect of Resistant Starch on HCl/ethanol-induced Gastric Injury in Rats

Yu Qian,Xin Zhao,Gui-Jie Li,Kai Zhu,Peng Sun,Xia Feng 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.5

Three types of resistant starch (RS) products were purchased for the evaluation of gastric injury preventive effect in Sprague-Dawley rats. We used an animal model to check for gastric injury preventive activities of these RS products in vivo. RS3 reduced the levels of serum proinflammatory cytokines of IL-6 and TNF-α as compared to those of RS2 and RS4. The gastric secretion volumes from high to low order were control rats, RS2-treated rats, RS4-treated rats, RS3-treated rats, and normal rats, whereas pH levels of gastric juice showed the opposite trend. The gastric injury level was significantly decreased by RS, demonstrating its anti-inflammatory properties, with RS3showing the best anti-inflammatory effect. Gastric tissues of RS3group rats showed significantly decreased mRNA and protein expression levels of inflammation-related genes of iNOS, COX-2,TNF-α, and IL-1β compared with the control group, as shown by RT-PCR and Western blot analyses. These results suggest that RS shows a gastric injury preventive effect, with RS3 showing the best inhibitory effect on gastric injury.

Effect of Resistant Starch on HCl/ethanol-induced Gastric Injury in Rats

Qian, Yu,Li, Guijie,Zhu, Kai,Sun, Peng,Feng, Xia,Zhao, Xin The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6

Three types of resistant starch (RS) products were purchased for the evaluation of gastric injury preventive effect in Sprague-Dawley rats. We used an animal model to check for gastric injury preventive activities of these RS products in vivo. RS3 reduced the levels of serum proinflammatory cytokines of IL-6 and TNF-${\alpha}$ as compared to those of RS2 and RS4. The gastric secretion volumes from high to low order were control rats, RS2-treated rats, RS4-treated rats, RS3-treated rats, and normal rats, whereas pH levels of gastric juice showed the opposite trend. The gastric injury level was significantly decreased by RS, demonstrating its anti-inflammatory properties, with RS3 showing the best anti-inflammatory effect. Gastric tissues of RS3 group rats showed significantly decreased mRNA and protein expression levels of inflammation-related genes of iNOS, COX-2, TNF-${\alpha}$, and IL-$1{\beta}$ compared with the control group, as shown by RT-PCR and Western blot analyses. These results suggest that RS shows a gastric injury preventive effect, with RS3 showing the best inhibitory effect on gastric injury.

중국 본토, 진폐증이 없는 석탄분진 근로자의 '삶의 질'

Yu, Hong-Mei,Ren, Xiao-Wei,Chen, Qian,Zhao, Jing-Yi,Zhu, Ting-Juan,Guo, Zhi-Xi 대한산업보건협회 2009 산업보건 Vol.250 No.-

Effects of Three Types of Resistant Starch on Intestine and Their Gastric Ulcer Preventive Activities in vivo

Yu Qian,Xin Zhao,Gui-Jie Li,Kai Zhu,Huayi Suo,Peng Sun 한국응용생명화학회 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6

To examine the effects of three types of resistant starch (RS) on intestinal metabolites, structure and their gastric injury preventive activities, Sprague-Dawley mice were fed diet containing 15% RS for 4 weeks. Response relationships among three types of RS (RS2, RS3, and RS4) food intake, body weight gain, food efficiency, wet weight of cecum (with or not with contents), pH, ammonia production, and short-chain fatty acids (SCFAs) concentration of cecal contents as well as intestinal structure were investigated. All three types of RS had effects on food intake, weight control, lowering of pH, ammonia production in cecal contents, increasing the wet weight of cecum (with or without contents), changes in SCFAs concentration of cecal contents, and physiological structure of small intestine and cecum compared to the control group (common starch-fed rats). RS3group showed minimum weight gain and food efficiency,compared to both the control group and the other two types of RS diet. RS3 group showed more significant lowering of pH,ammonia production, and SCFAs of cecal contents. In addition, all three types of RS shortened villous height, and muscle and mucosal thickening in small intestine, resulting in significantly decreased villous height and mucosal thickness, whereas significantly increased muscle thickness in the cecum. However,they did not cause detectable pathological changes in the small intestine and cecum. Animal model was also used to check its gastric ulcer preventive effects. The gastric ulcer level was significantly down-regulated by RS3, demonstrating its antiinflammatory properties. RS3 reduced the levels of serum proinflammatory cytokines of IL-6, IL-12, TNF-α, and IFN-γcompared to RS2 and RS4. RS3 increased the somatostatin (SS)and vasoactive intestinal peptide (VIP), decreased the motillin (MOT) and substance P (SP) serum levels. These results suggest that RS3 shows the best gastric ulcer preventive effect.

Effects of Three Types of Resistant Starch on Intestine and Their Gastric Ulcer Preventive Activities in vivo

Qian, Yu,Li, Gui Jie,Zhu, Kai,Suo, Hua Yi,Sun, Peng,Zhao, Xin The Korean Society for Applied Biological Chemistr 2013 Applied Biological Chemistry (Appl Biol Chem) Vol.56 No.6

To examine the effects of three types of resistant starch (RS) on intestinal metabolites, structure and their gastric injury preventive activities, Sprague-Dawley mice were fed diet containing 15% RS for 4 weeks. Response relationships among three types of RS (RS2, RS3, and RS4) food intake, body weight gain, food efficiency, wet weight of cecum (with or not with contents), pH, ammonia production, and short-chain fatty acids (SCFAs) concentration of cecal contents as well as intestinal structure were investigated. All three types of RS had effects on food intake, weight control, lowering of pH, ammonia production in cecal contents, increasing the wet weight of cecum (with or without contents), changes in SCFAs concentration of cecal contents, and physiological structure of small intestine and cecum compared to the control group (common starch-fed rats). RS3 group showed minimum weight gain and food efficiency, compared to both the control group and the other two types of RS diet. RS3 group showed more significant lowering of pH, ammonia production, and SCFAs of cecal contents. In addition, all three types of RS shortened villous height, and muscle and mucosal thickening in small intestine, resulting in significantly decreased villous height and mucosal thickness, whereas significantly increased muscle thickness in the cecum. However, they did not cause detectable pathological changes in the small intestine and cecum. Animal model was also used to check its gastric ulcer preventive effects. The gastric ulcer level was significantly down-regulated by RS3, demonstrating its anti-inflammatory properties. RS3 reduced the levels of serum proinflammatory cytokines of IL-6, IL-12, TNF-${\alpha}$, and IFN-${\gamma}$ compared to RS2 and RS4. RS3 increased the somatostatin (SS) and vasoactive intestinal peptide (VIP), decreased the motillin (MOT) and substance P (SP) serum levels. These results suggest that RS3 shows the best gastric ulcer preventive effect.

Combination of canagliflozin and puerarin alleviates the lipotoxicity to diabetic kidney in mice

Qian Zhu,Qu Zhou,Xiao-li Luo,Xu-jie Zhang,San-yu Li 대한약리학회 2023 The Korean Journal of Physiology & Pharmacology Vol.27 No.3

Diabetic kidney disease is one of the most serious complications of diabetes. Although diabetic kidney disease can be effectively controlled through strict blood glucose management and corresponding symptomatic treatment, these therapies cannot reduce its incidence in diabetic patients. The sodium-glucose cotransporter 2 (SGLT2) inhibitors and the traditional Chinese herb “Gegen” have been widely used in diabetes-related therapy. However, it remains unclear whether the combined use of these two kinds of medicines contributes to an increased curative effect on diabetic kidney disease. In this study, we examined this issue by evaluating the efficacy of the combination of puerarin, an active ingredient of Gegen, and canagliflozin, an SGLT2 inhibitor for a 12-week intervention using a mouse model of diabetes. The results indicated that the combination of puerarin and canagliflozin was superior to canagliflozin alone in improving the metabolic and renal function parameters of diabetic mice. Our findings suggested that the renoprotective effect of combined puerarin and canagliflozin in diabetic mice was achieved by reducing renal lipid accumulation. This study provides a new strategy for the clinical prevention and treatment of diabetic kidney disease. The puerarin and SGLT2 inhibitor combination therapy at the initial stage of diabetes may effectively delay the occurrence of diabetic kidney injury, and significantly alleviate the burden of renal lipotoxicity

Family History of Cancer and Head and Neck Cancer Risk in a Chinese Population

Huang, Yu-Hui Jenny,Lee, Yuan-Chin Amy,Li, Qian,Chen, Chien-Jen,Hsu, Wan-Lun,Lou, Pen-Jen,Zhu, Cairong,Pan, Jian,Shen, Hongbing,Ma, Hongxia,Cai, Lin,He, Baochang,Wang, Yu,Zhou, Xiaoyan,Ji, Qinghai,Zho Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.17

Background: The aim of this study was to investigate whether family history of cancer is associated with head and neck cancer risk in a Chinese population. Materials and Methods: This case-control study included 921 cases and 806 controls. Recruitment was from December 2010 to January 2015 in eight centers in East Asia. Controls were matched to cases with reference to sex, 5-year age group, ethnicity, and residence area at each of the centers. Results: We observed an increased risk of head and neck cancer due to first degree family history of head and neck cancer, but after adjustment for tobacco smoking, alcohol drinking and betel quid chewing the association was no longer apparent. The adjusted OR were 1.10 (95% CI=0.80-1.50) for family history of tobacco-related cancer and 0.96 (95%CI=0.75-1.24) for family history of any cancer with adjustment for tobacco, betel quid and alcohol habits. The ORs for having a first-degree relative with HNC were higher in all tobacco/alcohol subgroups. Conclusions: We did not observe a strong association between family history of head and neck cancer and head and neck cancer risk after taking into account lifestyle factors. Our study suggests that an increased risk due to family history of head and neck cancer may be due to shared risk factors. Further studies may be needed to assess the lifestyle factors of the relatives.

The alum-processing mechanism attenuating toxicity of Araceae Pinellia ternata and Pinellia pedatisecta

Hongli Yu,Hao Wu,Yaozong Pan,Xiuyun Ge,Qian Zhang,Fagen Zhu,Baochang Cai 대한약학회 2015 Archives of Pharmacal Research Vol.38 No.10

The present study aimed at investigating thealum-processing mechanism attenuating toxicity of AraceaePinellia ternata and Pinellia pedatisecta. Animalretroperitoneal inflammatory model in vivo andmacrophagocyte release inflammatory factor model in vitrowere used to detect the effect of alum processing onraphides and lectin. Scanning electron microscopy wasused to observe the change in raphides during processing;HPLC method was used to determine the correlation betweenthe dissolution and corrosion of raphides and ion inthe alum solution; 27Al-NMR technology was used to detectthe relationship between aluminum oxalate complexformation and the dissolved and corrosion of raphides. Thechange in protein peptide sequence of lectin during theprocessing of alum solution was determined by ShotgunLC–MS assay. Raphides induced severe rabbit conjunctivaledema and an intraperitoneal injection of lectin increasedPGE2 and protein in mice peritoneal exudate, whiledecreased after treatment with alum solution processing. During the processing raphides was dissolved and corroded,then its structure was damaged. Raphides was soaked inthe alum solution and significantly decreased the oxalatecontent, and the effect was related with Al3? in the alum. Al3? in the alum combined with C2O42- of raphides into astable complex compound promoted the dissolution ofcalcium oxalate. Raphides soaked in the alum made lectinproteins dissolve, whereas protein peptide sequence oflectin was changed and the protein structure was damaged. Conclusion: Alum solution could decrease the toxicity ofP. ternata (Thunb.) Breit. and P. pedatisecta Schott. Sinceit made a special crystal structure of raphides damage andthe protein of lectin dissolve. The structure of toxic substancessignificantly changed, which decreased the inflammatoryeffect.

Integral Attacks on Some Lightweight Block Ciphers

( Shiqiang Zhu ),( Gaoli Wang ),( Yu He ),( Haifeng Qian ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.11

At EUROCRYPT 2015, Todo proposed a new technique named division property, and it is a powerful technique to find integral distinguishers. The original division property is also named word-based division property. Later, Todo and Morii once again proposed a new technique named the bit-based division property at FSE 2016 and find more rounds integral distinguisher for SIMON-32. There are two basic approaches currently being adopted in researches under the bit-based division property. One is conventional bit-based division property (CBDP), the other is bit-based division property using three-subset (BDPT). Particularly, BDPT is more powerful than CBDP. In this paper, we use Boolean Satisfiability Problem (SAT)-aided cryptanalysis to search integral distinguishers. We conduct experiments on SIMON-32/-48/-64/-96, SIMON (102)-32/-48/-64, SIMECK-32/-48/-64, LBlock, GIFT and Khudra to prove the efficiency of our method. For SIMON (102)-32/-48/-64, we can determine some bits are odd, while these bits can only be determined as constant in the previous result. For GIFT, more balanced (zero-sum) bits can be found. For LBlock, we can find some other new integral distinguishers. For Khudra, we obtain two 9-round integral distinguishers. For other ciphers, we can find the same integral distinguishers as before.

Glutamic Acid Decarboxylase Autoantibody Detection by Electrochemiluminescence Assay Identifies Latent Autoimmune Diabetes in Adults with Poor Islet Function

Yuxiao Zhu,Li Qian,Qing Liu,Jing Zou,Ying Zhou,Tao Yang,Gan Huang,Zhiguang Zhou,Yu Liu 대한당뇨병학회 2020 Diabetes and Metabolism Journal Vol.44 No.2

Background: The detection of glutamic acid decarboxylase 65 (GAD65) autoantibodies is essential for the prediction and diagnosis of latent autoimmune diabetes in adults (LADA). The aim of the current study was to compare a newly developed electrochemiluminescence (ECL)-GAD65 antibody assay with the established radiobinding assay, and to explore whether the new assay could be used to define LADA more precisely. Methods: Serum samples were harvested from 141 patients with LADA, 95 with type 1 diabetes mellitus, and 99 with type 2 diabetes mellitus, and tested for GAD65 autoantibodies using both the radiobinding assay and ECL assay. A glutamic acid decarboxylase antibodies (GADA) competition assay was also performed to assess antibody affinity. Furthermore, the clinical features of these patients were compared. Results: Eighty-eight out of 141 serum samples (62.4%) from LADA patients were GAD65 antibody-positive by ECL assay. Compared with ECL-GAD65 antibody-negative patients, ECL-GAD65 antibody-positive patients were leaner (P<0.0001), had poorer β-cell function (P<0.05), and were more likely to have other diabetes-associated autoantibodies. The β-cell function of ECLGAD65 antibody-positive patients was similar to that of type 1 diabetes mellitus patients, whereas ECL-GAD65 antibody-negative patients were more similar to type 2 diabetes mellitus patients. Conclusion: Patients with ECL-GAD65 antibody-negative share a similar phenotype with type 2 diabetes mellitus patients, whereas patients with ECL-GAD65 antibody-positive resemble those with type 1 diabetes mellitus. Thus, the detection of GADA using ECL may help to identify the subtype of LADA.