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Immunohistochemical localization of nerve injury-induced protein-1 in mouse tissues
Poornima Ekanayake,Meejung Ahn,김정태,Taekyun Shin 대한해부학회 2019 Anatomy & Cell Biology Vol.52 No.4
Nerve injury-induced protein (Ninjurin)-1 is a cell adhesion molecule that is upregulated in neurons and Schwann cells after transection injury in rats. In this study, we investigated the localization of Ninjurin-1 in various tissues, including the cerebrum, sciatic nerve, spleen, lung, stomach, ileum, colon, liver, pancreas, kidney, testis, and skin in C57BL/6 mice, using Western blotting and immunohistochemistry. Western blot analysis showed that Ninjurin-1 was differentially expressed among organs. Ninjurin-1 was abundant in skin and ileum, weakly expressed in cerebrum, and moderately expressed in the other organs studied. Immunohistochemical analysis largely confirmed the results of the western blot analysis with often localization of Ninjurin-1 in the regions with abundant connective tissues. In addition, Ninjurin-1 was differentially expressed in various cell types in the tissues under the investigation. These findings suggest that Ninjurin-1 may play organ-specific roles in development and homeostasis of many organs.
Amelioration of experimental autoimmune encephalomyelitis by Ishige okamurae
안미정,김정태,양원준,최유나,Poornima Ekanayake,고현주,지영훈,신태균 대한해부학회 2018 Anatomy & Cell Biology Vol.51 No.4
Experimental autoimmune encephalomyelitis (EAE) is a T-cell‒mediated autoimmune central nervous system disease characterized by inflammation with oxidative stress. The aim of this study was to evaluate an anti-inflammatory effect of Ishige okamurae on EAE-induced paralysis in rats. An ethanolic extract of I. okamurae significantly delayed the first onset and reduced the duration and severity of hind-limb paralysis. The neuropathological and immunohistochemical findings in the spinal cord were in agreement with these clinical results. T-cell proliferation assay revealed that the ethyl-acetate fraction of I. okamurae suppressed the proliferation of myelin basic protein reactive T cells from EAE affected rats. Flow cytometric analysis showed TCRαβ+ T cells was significantly reduced in the spleen of EAE rats with I. okamurae treatment with concurrent decrease of inflammatory mediators including tumor necrosis factor-α and cyclooxygenase-2. Collectively, it is postulated that I. okamurae ameliorates EAE paralysis with suppression of T-cell proliferation as well as decrease of pro-inflammatory mediators as far as rat EAE is concerned.
김정태,안미정,최유나,Poornima Ekanayake,박철민,문창종,정경숙,Akane Tanaka,Hiroshi Matsuda,신태균 한국뇌신경과학회 2019 Experimental Neurobiology Vol.28 No.1
Olfactory dysfunction occurs in multiple sclerosis in humans, as well as in an animal model of experimental autoimmune encephalomyelitis (EAE). The aim of this study was to analyze differentially expressed genes (DEGs) in olfactory bulb of EAE-affected mice by next generation sequencing, with a particular focus on changes in olfaction-related signals. EAE was induced in C57BL/6 mice following immunization with myelin oligodendrocyte glycoprotein and adjuvant. Inflammatory lesions were identified in the olfactory bulbs as well as in the spinal cord of immunized mice. Analysis of DEGs in the olfactory bulb of EAE-affected mice revealed that 44 genes were upregulated (and which were primarily related to inflammatory mediators), while 519 genes were downregulated; among the latter, olfactory marker protein and stomatin-like 3, which have been linked to olfactory signal transduction, were significantly downregulated (log2 [fold change] >1 and p-value <0.05). These findings suggest that inflammation in the olfactory bulb of EAE-affected mice is associated with the downregulation of some olfactory signal transduction genes, particularly olfactory marker protein and stomatin-like 3, which may lead to olfactory dysfunction in an animal model of human multiple sclerosis.
Jeongtae Kim,Meejung Ahn,Yuna Choi,Poornima Ekanayake,Gi‑Ok Kim,Taekyun Shin 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.1
We evaluated the antifibrotic effects of fermented black radish (FBR) on carbon tetrachloride (CCl4)-induced chronic liver injury in rats. Serum biochemical analysis indicated that FBR treatment attenuated the upregulation of alanine aminotransferase and aspartate aminotransferase. Moreover, Sirius red staining and histopathological examination revealed decreased fibrosis and inflammation in FBR-treated rats compared with CCl4 + vehicle-treated rats. Western blot analysis of alpha-smooth muscle actin expression levels confirmed the downregulation of fibrosis in the FBR treatment group compared with the CCl4 + vehicle treatment group. These results suggest that FBR treatment suppressed liver fibrosis in CCl4-exposed rats.