셀 입구 예열방법에 따른 가압형 고체산화물 연료전지/가스터빈 하이브리드 시스템의 성능 비교 해석

양원준,김재환,김동섭 대한기계학회 2005 大韓機械學會論文集B Vol.29 No.6

수심의 제한을 받는 침수식생 개수로의 평균흐름 예측모형 개발

양원준,최성욱 한국수자원학회 2010 한국수자원학회논문집 Vol.43 No.9

침수식생 개수로 흐름은 식생영역과 상부영역에 서로 다른 흐름구조를 보인다. 즉, 식생영역에서 전단으로 인해 생성되는 난류는 억제되며 비교적 균일한 유속 분포를 보이며 상부영역에서는 일반 개수로 흐름과 유사한 흐름구조를 보인다. 이와 같이 두 상이한흐름구조가 결합된 복잡한 흐름특성으로 인해 침수식생 개수로흐름은 공학적인 관심의 대상이 되어왔다. 본 연구에서는 침수식생 개수로 흐름의 층적분 모형의 비교 분석을 수행하였다. 일반적으로 식생흐름의 층적분 모형은 Open-channel flows with submerged vegetation show two distinct flow structures in the vegetation and upper layers. That is, the flow in the vegetation layer is featured by relatively uniform mean velocity with suppressed turbulence from shear, while the f

Next-generation sequencing enables the discovery of more diverse positive clones from a phage-displayed antibody library

양원준,윤애린,이상훈,김수현,한정원,정준호 생화학분자생물학회 2017 Experimental and molecular medicine Vol.49 No.-

Phage display technology provides a powerful tool to screen a library for a binding molecule via an enrichment process. It has been adopted as a critical technology in the development of therapeutic antibodies. However, a major drawback of phage display technology is that because the degree of the enrichment cannot be controlled during the bio-panning process, it frequently results in a limited number of clones. In this study, we applied next-generation sequencing (NGS) to screen clones from a library and determine whether a greater number of clones can be identified using NGS than using conventional methods. Three chicken immune single-chain variable fragment (scFv) libraries were subjected to bio-panning on prostate-specific antigen (PSA). Phagemid DNA prepared from the original libraries as well as from the Escherichia coli pool after each round of bio-panning was analyzed using NGS, and the heavy chain complementarity-determining region 3 (HCDR3) sequences of the scFv clones were determined. Subsequently, through two-step linker PCR and cloning, the entire scFv gene was retrieved and analyzed for its reactivity to PSA in a phage enzyme immunoassay. After four rounds of bio-panning, the conventional colony screening method was performed for comparison. The scFv clones retrieved from NGS analysis included all clones identified by the conventional colony screening method as well as many additional clones. The enrichment of the HCDR3 sequence throughout the bio-panning process was a positive predictive factor for the selection of PSA-reactive scFv clones.

The different effects of gonadotropin-releasing hormone agonist therapy on body mass index and growth between normal-weight and overweight girls with central precocious puberty

양원준,고근혁,이곤희,황일태,오연정 대한소아내분비학회 2017 Annals of Pediatirc Endocrinology & Metabolism Vol.22 No.1

Purpose: The effects of gonadotropin-releasing hormone agonist (GnRHa) treatment on body mass index (BMI) are controversial in girls with central precocious puberty (CPP). We therefore evaluated auxological parameters during GnRHa therapy in patients with CPP, specifically focusing on changes in BMI. Methods: Seventy-seven girls with idiopathic CPP who underwent GnRHa therapy were retrospectively recruited. We investigated BMI changes during the treatment period after stratifying them according to baseline BMI status as follows: normal (BMI percentile of <85th) and overweight groups (BMI percentile of ≥85th). Results: The incidence of overweight/obesity (40.3%/23.4%) was very high in the girls with CPP. In the overall study population, no significant BMI change was observed during the GnRHa treatment period. However, when stratified according to baseline BMI status, the normal-weight group showed a significant increase in BMI-standard deviation score (SDS), whereas the overweight group showed no change in BMI-SDS. Baseline BMI-SDS was an independent predictor of changes in BMI during the GnRHa treatment period. Changes in weight-SDS were similar, but changes in height-SDS were significantly greater in the overweight group than in the normal-weight group, which explains the observed difference in BMI-SDS. Conclusion: Our results demonstrate that the difference in the pattern of BMI changes among our CPP patients suggests that delayed puberty induced by GnRHa treatment may have different effects on linear growth according to baseline body composition. This study underscores the importance of individualized lifestyle intervention in CPP children.

하천 퇴적물 중 입도에 따른 유기물질 분포 특성

양원준,정효진,한종학,조래현,양해종,김상돈,강태우,송상환,강태구 한국분석과학회 2016 학술대회논문집 Vol.2016 No.11

인공기포 존재 환경에서의 양상태 잔향음 예측을 위한 해상 실험 분석 및 모델링 연구

양원준,오래근,배호석,손수욱,김다솔,최지웅 한국음향학회 2022 韓國音響學會誌 Vol.41 No.4

Bubbles generated by various causes in the ocean are known to persist for long periods of time. Although the volume occupied by bubbles in the ocean is small, the presence of bubbles in ocean due to resonance and attenuation greatly affects the acoustic properties. Accordingly, bistatic reverberation experiment was performed in the ocean where artificial bubbles exist. A number of transducers and receivers were installed on 6 buoys arranged in a hexagonal shape, and blowing agents were dropped in the center of the buoy to generate bubbles. For reverberation modeling that reflects acoustic characteristics changed by bubbles, the spatial distribution of bubbles was estimated using video data and received signals. A measurement-based bubble spectral shape was used, and it was assumed that the bubble density within the spatial distribution of the estimated bubble was the same. As a result, it was confirmed that the bubble reverberation was simulated in a time similar to the measured data regardless of the bubble density, and the bubble reverberation level similar to the measured data was simulated at a void fraction of about 10-7 ~ 10-6.8. 해양에서 다양한 원인에 의해 발생된 기포들은 해수 중 오랜 시간 잔존하는 것으로 알려져 있다. 이러한 기포들이 해양환경에서 차지하고 있는 부피는 매우 작지만 공진, 감쇠 등으로 인해 해수 중 기포의 존재는 음향 특성에 큰 영향을 미친다. 이에 따라 본 논문에서는 인공기포가 존재하는 해양 환경에서의 양상태 잔향음 실험을 수행하였다. 다수의송수신기들이 육각형 형태로 배치된 6개의 부이에 설치되었으며, 부이 중앙에 발포제를 투하하여 인공기포를 발생시켰다. 발생된 기포에 의해 변화하는 음향 특성을 반영한 잔향음 모델링을 위해 영상자료와 수신신호를 이용하여 기포의 공간적인 분포를 추정하였다. 측정치 기반의 기포 분포 형태를 이용하였으며, 추정한 기포의 공간적 분포 내에서의기포 밀도는 동일하다고 가정하여 기포 밀도의 변화에 따른 모의 결과를 측정치와 비교, 분석하였다. 그 결과 기포에의한 잔향음 모의결과가 실측값과 유사한 시간대에 모의되었으며, 약 10-7 ~ 10-6.8의 기포율에서 실측값과 유사한 기포잔향음 준위가 모의됨을 확인하였다.

섬진강 수계 홍수전 퇴적물의 중금속 항목별 상관관계 분석

양원준,이재춘,정효진,김정현,임병진 한국환경분석학회 2013 제1회 한국분석기술단체 총 연합 합동 심포지엄 Vol.0 No.-

침수식생 개수로에서 난류 및 부유사 이동 특성

양원준,장지연,최성욱 한국수자원학회 2011 한국수자원학회논문집 Vol.44 No.5

침수식생 개수로 흐름은구조적으로상부영역과식생영역으로구분되는데, 상부영역은 일반 개수로의 흐름특성을 보이며 식생영역은 줄기에 의해 난류가 억제되어 정수식생 조건의 흐름과 유사하게 단순한 흐름특성을 보인다. 본 논문은 침수식생 개수로 흐름의 난류 및 유사이동 특성에 관한 실험연구이다. 이를 위해 폭 0.5m, 길이 12m인 개수로 실험장치를 이용하여수리실험을 실시하였다. 다양한 유량에 대하여 수심비 2~3인 흐름을 구현하였고, 나무원형 실린더를 이용하여 식 The open-channel flow with submerged vegetation shows distinct features in two separate regions, namely upper and vegetation layers. In the upper layer, the flow is akin to the open-channel flow, while the flow in the vegetation layer is relatively unifor

식생된 개수로 흐름의 난류 및 고유구조

양원준,최성욱 한국수자원학회 2002 한국수자원학회 학술대회 Vol.2002 No.1

가스터빈-가압형 SOFC 하이브리드 시스템의 성능특성 해석

양원준,김동섭,김재환 대한설비공학회 2004 설비공학 논문집 Vol.16 No.7

Recently, the hybrid system combining fuel cell and gas turbine has drawn much attention owing to its high efficiency and ultra low emission. It is now on the verge of world wide development and various system configurations have been proposed. A national project funded by Korean government has also been initiated to develop a pressurized hybrid system. This work aims at presenting design performance analysis for various possible system configurations as an initial step for the system development. Study focuses are given to major design options including the power ratio between gas turbine and fuel cell, reforming method (internal or external), reforming heat source (reforming burner, cathode hot air, fuel cell heat release) and steam supply method for reformer (anode gas recirculation, external steam generator). A wide variation in performance among different configurations has been predicted.