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Stilbenes and Oligostilbenes from Leaf and Stem of Vitis amurensis and Their Cytotoxic Activity
Do Thi Ha,Quan Cheng Chen,Tran Manh Hung,Tran Minh Ngoc,Phuong Thien Thuong,김홍진,성연희,민병선,배기환,윤의중 대한약학회 2009 Archives of Pharmacal Research Vol.32 No.2
Chromatographic separation of the EtOAc fraction from the leaf and stem of Vitis amurensis led to the isolation of six oligostilbenoids (i.e., r-2-viniferin (1), trans-amurensin B (2), trans-ε -viniferin (3), gnetinH (4), amurensin G (5), (+)-ampelopsin A (8)) and four stilbenoids (i.e., trans-resveratrol (6), (+)- ampelopsin F (7), piceatannol (9), and trans-piceid (10)). The structures have been identified on the basis of spectroscopic evidence and physicochemical properties. The isolates were investigated for cytotoxic activity against three cancer cell lines in vitro using the MTT assay method. Amurensin G (5) and trans-resveratrol (6) showed significant cytotoxic activity against L1210, K562 and HTC116 cancer cell lines with IC50 values ranging from 15.7 ± 2.1 to 30.9 ± 1.8 μM. (+)-Ampelopsin A (8) and trans-piceid (10) exhibited considerable cytotoxic activity against L1210 (IC50 values of 30.6 ± 4.1 and 28.7 ± 2.81μM, respectively) and K562 (IC50 values of 38.6 ± 0.82 and 24.6 ± 0.76 μM, respectively). Gnetin H (4)showed only weak cytotoxic activity against L1210 with an IC50 value of 40.1 ± 4.23 μM. On the other hand, r-2-viniverin (1), trans-amurensin B (2), trans-ε -viniferin (3), (+)-ampelopsin F (7), and piceatannol(9) exhibited no activity on three cancer cell lines.
Ngoc-Phuong Tran,박제권,Z-Hun Kim,이철균 한국생물공학회 2009 Biotechnology and Bioprocess Engineering Vol.14 No.3
Astaxanthin production is commonly induced under stress conditions such as nutrient deficiency (N or P), high light stress, and variations of temperature, high NaCl concentrations, and other factors. The objective of the present study is the analysis of the effect of oxidative stress by sodium orthovanadate (SOV), a nonspecific inhibitor of protein tyrosine phosphatases, on the cells growth and astaxanthin production of H.lacustris. In the presence of SOV (lower than 5.0 mM), maximum growth of H. lacustris obtained was 2.4 × 10 5 cells/mL in MBBM medium at 24°C under continuous illumination (40 μE/m 2 /s) of white fluorescent light, with continuous aeration of CO2 (0.2 vvm). Total carotenoids accumulated per cell biomass unit treated with 2.5 mM SOV has approximately shown 2.5 folds higher than the control after short period of SOV induction time as 2 days, despite that cells were grown under normal light. Meanwhile, maximal astaxanthin production from H.lacustris was 10.7 mg/g biomass in MBBM with 5 days of continuous illumination at 40 μE/m 2 /s, which has been established as optimal light intensity for the control culture of H.lacustris. Treating algae H.lcustris with sodium orthovanadate showed promoting the accumulation of astaxanthin by advancing either the inhibition of dephosphorylation or synthesis of ATP. Its potential role of PTPases in microalgae G.lacustris is discussed
Lipoxygenase Inhibitory Constituents from Rhubarb
Ngoc, Tran Minh,Minh, Pham Thi Hong,Hung, Tran Manh,Thuong, Phuong Thien,Lee, Ik-Soo,Min, Byung-Sun,Bae, Ki-Hwan 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5
Phytochemical study on the ethanol extract of rhubarb led to the isolation of fifteen compounds, including five anthraquinones: chrysophanol (1), physcion (2), emodin (7), chrysophanol-8-O-$\beta$-D-glucopyranoside (9) and emodin-8-O-$\beta$-D-glucopyranoside (15), and ten stilbenes: desoxyrhaponticin (3), rhaponticin (4), resveratrol (5), desoxyrhapotigenin (6), rhapontigenin (8), piceatannol-3'-O-$\beta$-D-glucopyranoside (10), piceid (11), $\varepsilon$-viniferin (12), ampelopsin B (13) and isorhaponticin (14). Their structures were identified by comparing the physicochemical data with those of published papers. Among the isolated compounds, stilbene derivatives (3-6, 8 and 10-14) showed remarkable inhibitory effect on lipoxygenase with $IC_{50}$ values ranging from 6.7 to $74.1\;{\mu}M$. The inhibition kinetics analyzed by Lineweaver-Burk plots found that they were competitive inhibitors with the linoleic acid at the active site of lipoxygenase. In addition, stilbenes exhibited significantly free radical scavenging activity against $ABTS^{\bullet+}$ with trolox equivalent activity capacity (TEAC) values ranging from 1.16 to 4.64. Whereas, anthraquinone derivatives (1-2, 7, 9 and 15) neither inhibited lipoxygenase nor scavenged free radical $ABTS^{\bullet+}$. These results indicated that stilbene derivatives were considerate to be mainly lipoxygenase inhibitor and free radical scavenger constituents of rhubarb.
NGOC, Tran Minh,HUNG, Tran Manh,THUONG, Phuong Thien,KIM, Jin-Cheon,CHOI, Jae Sue,BAE, KiHwan,HATTORI, Masao,CHOI, Chung-Sig,LEE, Joon Seok,MIN, Byung-Sun Japan Society for Bioscience, Biotechnology, and A 2008 Bioscience, Biotechnology, and Biochemistry Vol.72 No.8
<P>Two phenolics, 1,2,6-trigalloylglucose (<B>1</B>) and 1,2,3,6-tetragalloylglucose (<B>2</B>), isolated from the stem-bark of <I>Juglans mandshurica</I> were evaluated for their antioxidative activities. The results showed that compounds <B>1</B> and <B>2</B> exhibited strong scavenging activities against 1,1′-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzenthiazoline-6-sulphonic) acid (ABTS<SUP>•+</SUP>), and superoxide radicals (O<SUB>2</SUB><SUP>•−</SUP>), and also had a significant inhibitory effect on lipid peroxidation and low-density lipoprotein (LDL) oxidation. The strong superoxide radical scavenging of <B>1</B> and <B>2</B> resulted from the potential competitive inhibition with xanthine at the active site of xanthine oxidase (OX). In addition, compounds <B>1</B> and <B>2</B> displayed significant lipoxygenase inhibitory activity, the mode of inhibition also being identified as competitive. In comparison, the antioxidative activities of compounds <B>1</B> and <B>2</B>, together with gallic acid, indicated that the number of galloyl moieties could play an important role in the antioxidative activity.</P>
Lipoxygenase Inhibitory Constituents from Rhubarb
Tran Minh Ngoc,Pham Thi Hong Minh,Tran Manh Hung,IkSoo Lee,민병선,Phuong Thien Thuong,배기환 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.5
Phytochemical study on the ethanol extract of rhubarb led to the isolation of fifteen compounds, including five anthraquinones: chrysophanol (1), physcion (2), emodin (7), chrysophanol- 8-O-β-D-glucopyranoside (9) and emodin-8-O-β-D-glucopyranoside (15), and ten stilbenes: desoxyrhaponticin (3), rhaponticin (4), resveratrol (5), desoxyrhapotigenin (6), rhapontigenin (8), piceatannol-3'-O-β-D-glucopyranoside (10), piceid (11), ε -viniferin (12), ampelopsin B (13) and isorhaponticin (14). Their structures were identified by comparing the physicochemical data with those of published papers. Among the isolated compounds, stilbene derivatives (3-6, 8 and 10-14) showed remarkable inhibitory effect on lipoxygenase with IC50 values ranging from 6.7 to 74.1 μM. The inhibition kinetics analyzed by Lineweaver-Burk plots found that they were competitive inhibitors with the linoleic acid at the active site of lipoxygenase. In addition, stilbenes exhibited significantly free radical scavenging activity against ABTS•+ with trolox equivalent activity capacity (TEAC) values ranging from 1.16 to 4.64. Whereas, anthraquinone derivatives (1-2, 7, 9 and 15) neither inhibited lipoxygenase nor scavenged free radical ABTS•+. These results indicated that stilbene derivatives were considerate to be mainly lipoxygenase inhibitor and free radical scavenger constituents of rhubarb.
Dieu Linh Tran,Anh Phuong Nguyen Hong,Ngoc Hoi Nguyen,Ngoc Trinh Huynh,Bao Ha Le Tran,Cam Tu Tran,Minh Dung Truong,Quan Dang Nguyen,박기동,Dai Hai Nguyen 한국공업화학회 2023 Journal of Industrial and Engineering Chemistry Vol.120 No.-
α-calcium sulfate hemihydrate (α-HH) was synthesized by salt solution methods to prepare a promising biomaterial for bone tissue repair and regeneration. The successful synthesis of α-HH was evaluated by field emission scanning electron microscopy (FE-SEM), thermogravimetric analysis (TGA), X-ray diffraction (XRD), and Fourier transform infrared (FTIR) scanning. The sterility of α-HH before and after irradiation with gamma ray was firstly confirmed by Colonies Forming Units (CFU) counting assay, to target the surgical grade application. In vitro tetrazolium bromide (MTT) assay, crystal violet (CV) and acridine orange (AO) staining was performed to assess the initial cytotoxicity and cell attachment ability of α-HH. Further in vivo implantation into rabbit distal femoral condyles defect exhibited the ability of salt solution-synthesized α-HH to promote the localization of osteocytes and osteoblasts, which improve the bone tissue repair and regeneration. The findings suggested that α-calcium sulfate hemihydrate synthesized by salt solution method is a potential material that can be used as bone substitutes.
Chien Minh Tran,Ngoc Thi‑Thanh Nguyen,Minh Hieu Ho,Vinh Khanh Doan,Khanh Loan Ly,Nhi Ngoc‑Thao Dang,Nam Minh‑Phuong Tran,Hoai Thi‑Thu Nguyen,Long Phuoc Truong,Thai Minh Do,Quyen Ngoc Tran,Hien Quoc Ng 한국섬유공학회 2023 Fibers and polymers Vol.24 No.1
In this study, we proposed a straightforward electrospun polycaprolactone (PCL) loaded with silver nanoparticles (SNPs)membrane fabrication process, in which SNPs were directly synthesized from silver nitrate (AgNO3) in PCL–acetone mixtureby gamma irradiation. The insolubility of AgNO3in PCL solution was solved using an auxiliary dimethyl sulfoxide solvent. As a physical approach, gamma rays readily converted silver ions into SNPs without the addition of harmful reductionagents, which reduced the cytotoxicity of the synthesized material. By avoiding some processes such as purification, solventremoval, or redispersion of SNPs, this method was more time-saving compared to other related studies. SNPs formation wasconfirmed by both UV–Visible spectrum (UV–Vis) and X-ray diffraction analysis. Scanning electron microscopy (SEM)revealed that the addition of SNPs significantly reduced the fiber diameter of PCL–Ag membranes compared to that of rawPCL. Uniform spherical-shaped SNPs incorporated in PCL fibers were observed under transmission electron microscopy(TEM). The tensile test showed that the electrospun PCL–Ag membranes exhibited good mechanical characteristics. Moistureeasily penetrated the porous microstructure of PCL–Ag, facilitating wound humidity regulation. Inductively coupledplasma-mass spectroscopy (ICP-MS) was employed to study the release profiles of SNPs at different time intervals. Overall,the PCL–Ag 500 ppm sample exerted excellent antibacterial activity against Pseudomonas aeruginosa and Staphylococcusaureus strains and low in vitro cytotoxicity.
Tran Thi Huyen,Ha Phuong Trang,Nguyen Thi-Ngan,Bui Dinh-Thanh,Le Pham Tan Quoc,Trinh Ngoc Nam 한국수산과학회 2023 Fisheries and Aquatic Sciences Vol.26 No.3
The thermolabile haemolysin (tlh) of Vibrio parahaemolyticus (Vptlh) from V. parahaemolyticus is a multiple-function enzyme, initially describes as a haemolytic factor activated by lecithin and phospholipase A2 enzymatic activity (Shinoda, 1991; Vazquez-Morado, 2021; Yanagase et al., 1970). Until now, the tlh structure has hypothesized including N-terminal and C-terminal domain, but what domain of the Vptlh structure does the haemolytic activity has not been refined yet. In this study, a 450-bp VpTLH nucleotide sequence of the entire Vptlh gene encoded the C-terminal domain cloned firstly to examine its responsibility in the activity of the Vptlh. The C-terminal domain fused with a 6-His-tag named the His-tag-VpC-terminal domain was expressed successfully in soluble form in the BL21 (DE3) PlysS cell. Remarkably, both expression and purification results confirmed a high agreement in the molecular weight of the His-tag-VpC-terminal domain was 47 kDa. This work showed the His-tag-VpC-terminal domain lysed the erythrocyte membranes in the blood agar and the phosphate buffered saline (0.9%) media without adding the lecithin substrate of the phospholipase enzyme. Haemolysis occurred at all tested diluted concentrations of His-tag-VpC-terminal domain (p < 0.05), providing evidence for the independent haemolytic activity of the His-tag-VpC-terminal domain. The content of 100 μg of the His-tag-VpC-terminal domain brought the highest haemolytic activity of 80% compared to that in the three remaining contents. Significantly, the His-tag-VpC-terminal domain demonstrated not to involve the phospholipase activity in Luria-Bertani agar supplemented with 1% (vol/vol) egg yolk emulsion. All results proved the vital responsibility of the His-tag-VpC-terminal domain in causing the haemolytic activity without the required activation by the phospholipase enzyme. Raw extracts of Phellinus igniarus and Phellinus pipi at 10–1 mg/mL inhibited the haemolytic activity of the His-tag-VpC-terminal domain from 67.7% to 87.42%, respectively. Hence applying the His-tag-VpC-terminal domain as a simple biological material to evaluate quickly potential derivatives against the Vptlh in vivo conditions will accessible and more advantageous than using the whole of the Vptlh.