Influence of Sodium Orthovanadate on the Production of Astaxanthin from Green Algae Haematococcus lacustris

다국어 초록 (Multilingual Abstract)

Astaxanthin production is commonly induced under stress conditions such as nutrient deficiency (N or P), high light stress,
and variations of temperature, high NaCl concentrations, and other factors. The objective of the present study is the analysis
of the effect of oxidative stress by sodium orthovanadate (SOV), a nonspecific inhibitor of protein tyrosine phosphatases, on
the cells growth and astaxanthin production of H.lacustris. In the presence of SOV (lower than 5.0 mM), maximum growth
of H. lacustris obtained was 2.4 × 10
5
cells/mL in MBBM medium at 24°C under continuous illumination (40 μE/m
2
/s) of
white fluorescent light, with continuous aeration of CO2 (0.2 vvm). Total carotenoids accumulated per cell biomass unit
treated with 2.5 mM SOV has approximately shown 2.5 folds higher than the control after short period of SOV induction time
as 2 days, despite that cells were grown under normal light. Meanwhile, maximal astaxanthin production from H.lacustris
was 10.7 mg/g biomass in MBBM with 5 days of continuous illumination at 40 μE/m
2
/s, which has been established as optimal
light intensity for the control culture of H.lacustris. Treating algae H.lcustris with sodium orthovanadate showed promoting
the accumulation of astaxanthin by advancing either the inhibition of dephosphorylation or synthesis of ATP. Its potential
role of PTPases in microalgae G.lacustris is discussed

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