패혈증의 경과에 따른 혈중 스테로이드와 Nitric Oxide의 연관성

이규성 ( Keu Sung Lee ),김영선 ( Young Sun Kim ),이형노 ( Hyoung No Lee ),박주헌 ( Joo Hun Park ),오윤정 ( Yoon Jung Oh ),신승수 ( Seung Soo Sheen ),최영화 ( Young Hwa Choi ),박광주 ( Kwang Joo Park ),황성철 ( Sung Chul Hwang ) 대한결핵 및 호흡기학회 2007 Tuberculosis and Respiratory Diseases Vol.62 No.4

연구배경: 패혈증에서 Nitric oxide 스테로이드 호르몬은 혈역학적 변화와 염증반응에 관여하는데 이 두 인자는 서로 상관성이 있는 것으로 알려지고 있다. 하지만 실제 환자에서 서로의 상관성이나 임상적 의의는 연구가 부족한 실정이다. 방법: 패혈증 환자 26예와 대조군 14예를 대상으로 혈중 총 NO와 혈중 코티졸 농도를 측정하였고 이어서 제 3, 5, 7병일에도 연속적으로 측정을 하였다. 결과: 패혈증 환자군에서 초기 혈중 코티졸 및 총 NO 농도는 대조군에 비하여 유의하게 증가하였고 경증 패혈증에 비하여 중증 패혈증 환자군에서 유의하게 높았다. 초기 혈중 총 NO의 농도는 APACHE II 점수, 정맥혈 lactate 농도와 상관성이 있었다. 패혈증의 시간의 경과에 따라 혈중 NO 농도는 제 1병일, 제 5병일, 제 7병일에 혈중 코티졸의 농도와 유의한 상관성이 있었다. 결론: 패혈증 환자들에서 혈중 NO와 코티졸 농도는 증가되어 있었으며, 경과에 따라 서로 유의한 상관성이 지속되었다. 상호작용기전에 대하여는 추가적인 연구가 필요할 것이다. Background: The nitric oxide (NO) released by inducible NO synthase (iNOS) plays an important role in the pathophysiology of sepsis. Corticosteroids also play a role in the hemodynamic and inflammatory reactions in sepsis. Both have been shown to have a relationship theoretically, but their correlation and clinical impacts have rarely been evaluated. Methods: 26 patients with sepsis and 14 healthy controls were enrolled in this study. The initial random plasma total NO and the serum cortisol levels were measured. The same measurements were serially carried out on the 3rd, 5th, and 7th days. Results: The initial total plasma levels of NO and cortisol were higher in the patients with sepsis than in the healthy controls. The total NO levels were higher in patients with severe sepsis than in the those with mild sepsis. There was a correlation between the total NO and cortisol level throughout the study. Conclusion: In patients with sepsis, the levels of plasma NO and cortisol were well correlated during the first week of sepsis, which suggests an interrelationship. However, the clinical and pathogenetic implications await further evaluation. (Tuberc Respir Dis 2007; 62: 308-313)

Production of L-Theanine Using Escherichia coli Whole-Cell Overexpressing γ-Glutamylmethylamide Synthetase with Baker’s Yeast

( Soo-yeon Yang ),( Yeong-hoon Han ),( Ye-lim Park ),( Jun-young Park ),( So-young No ),( Daham Jeong ),( Saerom Park ),( Hyung Yeon Park ),( Wooseong Kim ),( Seung-oh Seo ),( Yung-hun Yang ) 한국미생물 · 생명공학회 2020 Journal of microbiology and biotechnology Vol.30 No.5

L-Theanine, found in green tea leaves has been shown to positively affect immunity and relaxation in humans. There have been many attempts to produce L-theanine through enzymatic synthesis to overcome the limitations of traditional methods. Among the many genes coding for enzymes in the L-theanine biosynthesis, glutamylmethylamide synthetase (GMAS) exhibits the greatest possibility of producing large amounts of production. Thus, GMAS from Methylovorus mays No. 9 was overexpressed in several strains including vectors with different copy numbers. BW25113(DE3) cells containing the pET24ma::gmas was selected for strains. The optimal temperature, pH, and metal ion concentration were 50℃, 7, and 5 mM MnCl₂, respectively. Additionally, ATP was found to be an important factor for producing high concentration of L-theanine so several strains were tested during the reaction for ATP regeneration. Baker’s yeast was found to decrease the demand for ATP most effectively. Addition of potassium phosphate source was demonstrated by producing 4-fold higher L-theanine. To enhance the conversion yield, GMAS was additionally overexpressed in the system. A maximum of 198 mM L-theanine was produced with 16.5 mmol/l/h productivity. The whole-cell reaction involving GMAS has greatest potential for scale-up production of L-theanine.

L1210 세포증식에 대한 Glycyrrhizin의 억제작용 기전

殷載淳,徐龍勳,權鎭,柳東和,吳贊鎬,蘇俊魯,全焄,黃甲洙 우석대학교 의약품개발연구소 1996 藥學硏究誌 Vol.1 No.-

The purpose of this research was to investigate the mechanism of inhibitory action of Glycyrrhizin(GZ) on the proliferation of mouse leukemia cell-line, L1210 cells. The cytotoxic activity was tested using a colorimetric tertrazolium assay(MTT assay), the apoptosis was tested using flow cytometry. Nitric oxide(NO) production form mouse peritoneal macrophage was tested using a Griess method and the phagocytosis of human polymorphonuclear cells was tested using a lucigenin chemiluminescence. GZ ingibited the proliferation of L1210, BALB/c 3T3 cells and mouse thymocytes at 50 ug/ml/ GZ did not affect nitric oxide production from mouse peritoneal macrophages in vitro, but ingibited nitric oxide production from lipopolysaccharide and y-interferon treated macrophages. Macrophages of GZ-administered mice accelerated NO production. The proliferation of L1210 cells apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. The apoptosis of L1210 cells were induced by co-culture with macrophage of GZ-administered mice. GZ increased the phagocytosis of human polymorphonuclear cells. These results suggest that GZ inhibit the proliferation of L1210 cells not only as a direct cytotoxic agent o tumor cells, but also by the enhancement of NO production and phagocytic activity.

소득작물 전후작지 적응 극단기성 벼 ‘중모 1032’

손영보,이종희,조준현,이지윤,오성환,송유천,박동수,박노봉,김상열,여운상,남민희,권영업 忠北大學校 農業科學硏究所 2015 農業科學硏究 Vol.31 No.2

중심압축력을 받는 高强度 RC기둥의 띠철근 配筋形態에 關한 實驗的 硏究

이영인,이영호,곽노현,정헌수 중앙대학교 기술과학연구소 1998 기술과학연구소 논문집 Vol.28 No.-

An objective of this study is to experimentally investigate the strength and ductility of reinforced concrete columns under uniaxial load and several test variables. To do this, we have conducted tests on twelve 20×20×60cm specimens with 8 and 12 longitudinal steel bars subjected to monotonic uniaxial compression. The main variables considered in this test are the configuration of ties, the strength of concrete, and the space of ties. The results indicate that the strength and the ductility of reinforced concrete columns have been influenced on the configuration of ties, the space of ties, and the strength of concrete.

中心壓軸力을 받는 高强度 鐵筋콘크리트 기둥의 耐力과 延性에 關한 실험적 硏究

곽노현,이영호,김남주,이경종,정헌수 중앙대학교 기술과학연구소 1997 기술과학연구소 논문집 Vol.27 No.-

A study on the behavior of short reinforced concrete columns was experimently described. Twenty-four concrete columns, with 20cm square and 60cm height, containing 8 longitudinal steel bars and different the amount of ties, spacing and concrete strengths, were subjected to concentric load by failure. Test results indicated that 1) It is incompatible in high-strength concrete to arrange stirrups to the AIK code of D/2 throughout the example of 10cm stirrup spacing following AIK codes. 2) As the amount of stirrup increased, the strength and ductily of reinforced concrete column improved. 3) As the concrete strength is high, the effect of the was reduced. 4) If strength enhancement factors increased, strain at maximum load also does and ductility ratios does almost linearliary. 5) The rate of strength enhancement factor does well with the modified kent & Park's theory. 6) As to the result of calculating the effective confined area which is suggested in Sheikh & Uzumeri's theory, the middle vertical steel is effective, only if the stirrups are arranged with spacing of 3cm.

고등급 보안 시스템을 위한 IPSec 시스템 기능 명세

유희준,김영미,최진영,서동수,노병규 고려대학교 컴퓨터과학기술연구소 2001 JOURNAL OF COMPUTER SCIENCE & ENGINEERING TECHNOLO Vol.3 No.-

현대 사회는 인터넷 사용자의 증가와 제반 기술의 비약적인 발전으로 사회활동 전반에 대한 인터넷 기반 서비스가 이루어지고 있다. 이런 과정에서 발생한 중요한 문제중의 하나가 불특정 다수가 자유로이 통신을 하기 위해 개발된 인터넷상에서 사용자의 보안을 보장해주는 것이다. 최근에 와서 인터넷상에서 개인 정보유출로 인한 많은 피해가 발생하면서 정보보호에 대한 관심이 더욱 증가하고 있다. 현재 이 문제를 해결하기 위하여 많은 보안 관련 시스템들이 개발되고 있으며, 세계 각 국의 표준 기구에서는 이러한 시스템에 대한 등급을 평가하고 있다. 각 등급을 살펴보면, 시스템 개발 초기부터 정형기법을 이용하여 개발되어진 시스템들이 고등급을 획득하고 있다. 국내에서도 한국정보보호센터(KISA)에서 보안 시스템에 대한 등급을 평가를 수행하면서 정형기법을 이용하여 땅세 검증된 시스템에게 K5이상의 고등급을 부여하고 있다. 이러한 등급을 살펴보면, 시스템의 기능을 정형명세언어를 이용해서 명세하는 부분을 공통적으로 요구하고 있다. 본 논문에서는 높은 보안 등급의 IPSec 관련 시스템을 개발하기 위해서 정형명세언어인 Z를 이용해서 IPSec의 동작을 명세하였다. The modern community is moving across the whole areas based on Internet with the growing Internet users and the great technological development. One of the important things that can be problem in this community is that the Internet should assure secure networking and security mechanism for the many security-ignorant users. Recently, Due to a lot of damages like a personal drain, security concerns is growing very rapidly. Now, many security application system is developing to solve this problem and each of the world standard organizations is evaluating these security application systems. Considering the each levels, we found that system using formal methods from the initial system development process acquired the high-level. In domestic, KISA manages the evaluation of security system and gives the higher than K5 to the system that specified and verified using formal methods. In this all levels, it should be required the system specification part. In this paper, we specified IPSec using formal specification language Z to develope the high-level IPSec system.

SPRAY AND COMBUSTION CHARACTERISTICS OF HYDROCARBON FUEL INJECTED FROM PRESSURE-SWIRL NOZZLES

Laryea Gabriel Nii,No Soo-Young The Institute for Liquid Atomization and Spray Sys 2004 한국액체미립화학회지 Vol.9 No.4

This paper presents spray and combustion characteristics of hydrocarbon fuel injected from pressure-swirl nozzles. Three commercial nozzles with orifice diameters of 0.256, 0.308 and 0.333mm and injection pressures ranging from 0.7 to 1.3 MPa were selected f9r the experiments. Spray characteristics such as breakup length. spray angle and drop size (SMD) were analyzed using photo image analyses and Malvern Panicle Size Analyzer. The drop size was measured with and without a blower at the same measuring locations. The flame length and width were measured using photo image analyses. The temperature distribution along the axial distance and the gas emission such as CO, $CO_2\;and\;NO_x$ were studied. The breakup length decreased with an increase in injection pressure for each nozzle but increased with an increase in nozzle orifice diameter. The spray angle increased and SMD decreased with an increase in injection pressure. The flame with an increased linearly with an increase in injection pressure and in nozzle orifice diameter. The flame temperature increased with an increase in injection pressure but decreased along the axial distance. The maximum temperatures occurred closer to the burner exit and flame at axial distance of 242mm from the diffuser tip. The experimental results showed that the level of CO decreased while that of $CO_2\;and\;NO_x$ increased with an increase in injection pressure and nozzle orifice diameter.

구속된 고강도 콘크리트 기둥의 휨 거동에 관한 실험적 연구

양근혁,이영호,곽노현,정헌수 중앙대학교 기술과학연구소 1998 기술과학연구소 논문집 Vol.28 No.-

본 실험적 연구의 목적은 콘크리트 강도, 띠근 배근형태, 띠근 량, 축하중비가 축하증과 횡하증을 받는 구속된 콘크리트 기둥의 휨 내력과 연성에 미치는 영향을 팡가하기 위한 것이다. 구속된 기둥의 휨 거동을 시험하기 위하여 200×200×800mm 크기의 시험체 14개를 2축 하중하에서 실험하였다. 시험결과로서, 저축력에서 콘크리트 구속으로 인한 휨 강도의 증가는 비교적 작게 있지만, 고축력에서 콘크리트 구속으로 인한 휨 강도 증가는 크게 있다. 그리고, 고축력, 고강도 콘크리트에서 내부 cross-ties가 있으면 휨 연성의 증가가 보였으며, 구속된 콘크리트 기둥의 연성은 단순한 띠근 량보다는 구속지수에 관계되었다. The purpose of this experimental study was to investigate the effects of concrete strength, the configuration of lateral ties, the amount of lateral ties, and level of axial loads on flexural strength and ductility of confined of reinforced concrete columns subjected to combined axial and lateral loads. To simulate the flexural behavior of confined columns, fourteen reinforced concrete columns, 200×200×800mm, were tested under bi-axial loads. Test results indicate that for low axial load levels, the enhancement of the flexural strength due to concrete confinement is relatively small, but that for high axial load levels, the enhancement of the flexural strength due to concrete confinement can be very high. Considerable enhancement of ductility was observed in columns confined by cross-ties, even for high strength concrete columns and under high axial loads. Ductility of confined columns are found to be more related to confinement index (ρ??f??/??) than the amount of lateral ties(ρ??)

Aspirin 諸合性에 關한 再考

김정란,박숙자,유영자,박옥운,김정은,노수자,오경희,조연자 梨花女子大學校 藥學硏究所 1962 梨花藥學硏究 Vol.- No.3