The differentiation of human multipotent adult progenitor cells into hepatocyte-like cells induced by coculture with human hepatocyte line L02

Ning Mu,Hong-Bao Liu,Qiu-Hong Meng,De-Wei Du,Yi Jiang,Huan-Zhang Hu 대한외과학회 2014 Annals of Surgical Treatment and Research(ASRT) Vol.88 No.1

Purpose: The aim of this study was to establish an in vitro method to purify human multipotent adult progenitor cells (hMAPCs) and assess their possible differentiation into hepatocytes by coculture with human hepatocyte line L02. Methods: hMAPCs were isolated by magnetic activated cell sorting (MACS) depletion selection using CD45 and GlyA microbeads. After indirect or direct coculture of hMAPCs and human hepatocyte line L02, the expression of albumin (ALB), alpha-fetoprotein (AFP), cytokeratin (CK) 18, and CK19 by hMAPCs was detected by immunocytochemistry. Results: With the MACS method, (5?10) × 10⁴/mL hMAPCs could be separated from 1 × 10?/mL bone marrow mononuclear cells. The purity of CD45-/GlyA- cells separated from bone marrow adherent cells was more than 98%, as determined by flow cytometry. In the coculture without cell-to-cell contact, hMAPCs expressed high AFP on day 1, and then tapered daily to low expression on day 7; ALB expression reached its peak on day 5, and remained high on day 7; CK18 was initially expressed on day 5 and was higher on day 7; CK19 was negative in all assays. In the coculture with cell-to-cell contact, ALB and CK18 were expressed by most cells while AFP appeared in only a few on day 5. Conclusion: hMAPCs were induced to differentiate into mature hepatocyte-like cells by coculture with a hepatocyte cell line, either with or without cell-to-cell contact, but the former seemed more effective.

A New Fuzzy Key Generation Method Based on PHY-Layer Fingerprints in Mobile Cognitive Radio Networks

( Ning Gao ),( Xiaojun Jing ),( Songlin Sun ),( Junsheng Mu ),( Xiang Lu ) 한국인터넷정보학회 2016 KSII Transactions on Internet and Information Syst Vol.10 No.7

Classical key generation is complicated to update and key distribution generally requires fixed infrastructures. In order to eliminate these restrictions researchers have focused much attention on physical-layer (PHY-layer) based key generation methods. In this paper, we present a PHY-layer fingerprints based fuzzy key generation scheme, which works to prevent primary user emulation (PUE) attacks and spectrum sensing data falsification (SSDF) attacks, with multi-node collaborative defense strategies. We also propose two algorithms, the EA algorithm and the TA algorithm, to defend against eavesdropping attacks and tampering attacks in mobile cognitive radio networks (CRNs). We give security analyses of these algorithms in both the spatial and temporal domains, and prove the upper bound of the entropy loss in theory. We present a simulation result based on a MIMO-OFDM communication system which shows that the channel response characteristics received by legitimates tend to be consistent and phase characteristics are much more robust for key generation in mobile CRNs. In addition, NIST statistical tests show that the generated key in our proposed approach is secure and reliable.

Antioxidant Properties of Proanthocyanidins Attenuate Carbon Tetrachloride (CCl4)–Induced Steatosis and Liver Injury in Rats via CYP2E1 Regulation

Ning Dai,Yuan Zou,Lei Zhu,Hui-Fang Wang,Mu-Gen Dai 한국식품영양과학회 2014 Journal of medicinal food Vol.17 No.6

Liver steatosis is characterized by lipid dysregulation and fat accumulation in the liver and can lead tooxidative stress in liver. Since proanthocyanidins are present in plant-based foods and have powerful antioxidant properties,we investigated whether proanthocyanidins can prevent oxidative stress and subsequent liver injury. Carbon tetrachloride(CCl4) treatment can cause steatosis in rats that models both alcoholic and non-alcoholic fatty liver disease in humans. Wepre-treated rats by oral administration of proanthocyanidins extracted from grape seeds 7 days prior to intragastricallyadministering CCl4. Proanthocyanidin treatment continued for an additional 2 weeks, after which time liver and serum were harvested, and mediators of liver injury, oxidative stress, and histological features were evaluated. CCl4-treated rats exhibited significant increases in the following parameters as compared to non-treated rats: fat droplets in the liver, liver injury (ALT, AST), and DNA damage (8-OHdG). Additionally, CCl4 treatment decreased antioxidant enzymes SOD, GSH, GPX, and CAT in the liver due to their rapid depletion after battling against oxidative stress. Compared to CCl4-treated rats, treatment with proanthocyanidins effectively suppressed lipid accumulation, liver injury, DNA damage, as well as restored antioxidant enzyme levels. Further investigation revealed that proanthocyanidins treatment also inhibited expression of CYP2E1 in liver, which prevented the initial step of generating free radicals from CCl4. The data presented here show that treatment with orally administered proanthocyanidins prevented liver injury in the CCl4-induced steatosis model, likely through exerting antioxidant actions to suppress oxidative stress and inhibiting the free radical–generating CYP2E1 enzyme.

Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA

Ning Xue,Jian-Hua Lin,Shan Xing,Dan Liu,Shi-Bing Li,Yan-Zhen Lai,Xue-Ping Wang,Min-Jie Mao,Qian Zhong,Mu-Sheng Zeng,Wan-Li Liu 대한암학회 2019 Cancer Research and Treatment Vol.51 No.1

Purpose The purpose of this study was to identify novel plasma biomarkers for distinguishing nasopharyngeal carcinoma (NPC) patients from healthy individuals who have positive Epstein-Barr virus (EBV) viral capsid antigen (VCA-IgA). Materials and Methods One hundred seventy-four plasma cytokines were analyzed by a Cytokine Array in eight healthy individuals with positive EBV VCA-IgA and eight patients with NPC. Real-time polymerase chain reaction, Western blotting, enzyme-linked immunosorbent assay (ELISA), and immunohistochemistry were employed to detect the expression levels of macrophage migration inhibitory factor (MIF) and CC chemokine ligand 3 (CCL3) in NPC cell lines and tumor tissues. Plasma MIF and CCL3 were measured by ELISA in 138 NPC patients, 127 EBV VCA-IgA negative (VN) and 100 EBV VCA-IgA positive healthy donors (VP). Plasma EBV VCA-IgA was determined by immunoenzymatic techniques. Results Thirty-four of the 174 cytokines varied significantly between the VP and NPC group. Plasma MIF and CCL3 were significantly elevated in NPC patients compared with VN and VP. Combination of MIF and CCL3 could be used for the differential diagnosis of NPC from VN cohort (area under the curve [AUC], 0.913; sensitivity, 90.00%; specificity, 80.30%), and combination of MIF, CCL3, and VCA-IgA could be used for the differential diagnosis of NPC from VP cohort (AUC, 0.920; sensitivity, 90.00%; specificity, 84.00%), from (VN+VP) cohort (AUC, 0.961; sensitivity, 90.00%; specificity, 92.00%). Overexpressions of MIF and CCL3 were observed in NPC plasma, NPC cell lines and NPC tissues. Conclusion Plasma MIF, CCL3, and VCA-IgA combination significantly improves the diagnostic specificity of NPC in high-risk individuals.

Energy Efficiency Resource Allocation for MIMO Cognitive Radio with Multiple Antenna Spectrum Sensing

( Bing Ning ),( Shouyi Yang ),( Xiaomin Mu ),( Yanhui Lu ),( Wanming Hao ) 한국인터넷정보학회 2015 KSII Transactions on Internet and Information Syst Vol.9 No.11

The energy-efficient design of sensing-based spectrum sharing of a multi-input and multi-output (MIMO) cognitive radio (CR) system with imperfect multiple antenna spectrum sensing is investigated in this study. Optimal resource allocation strategies, including sensing time and power allocation schemes, are studied to maximize the energy efficiency (EE) of the secondary base station under the transmit power and interference power constraints. EE problem is formulated as a nonlinear stochastic fractional programming of a nonconvex optimal problem. The EE problem is transformed into its equivalent nonlinear parametric programming and solved by one-dimension search algorithm. To reduce searching complexity, the search range was founded by demonstration. Furthermore, simulation results confirms that an optimal sensing time exists to maximize EE, and shows that EE is affected by the spectrum detection factors and corresponding constraints.

PtPd/molecular sieve as dual-functional monolithic adsorbent/catalyst for effective removal of trace toluene at low-temperature and their electric-heating performance

Shuangde Li,Tao Wang,Mu Zhou,Shaohua Chai,Linfeng Nie,Zhenjiang Wu,Ning Han,Yunfa Chen 한국공업화학회 2022 Journal of Industrial and Engineering Chemistry Vol.114 No.-

A dual-functional adsorption–catalytic system for elimination of trace toluene is developed and realizedover molecular sieve-hosted varied ratio of platinum and palladium catalysts. PtPd/molecular sieveexhibites PdO and PtO2 around 2–3 nm particles with uniform loading verified by XRD, TEM, Ramanand XPS. The adsorption–desorption–catalysis for trace toluene purification is evaluated on the quartzmicro-reactor and the self-made electrically heated equipment. 3Pt1Pd/molecular sieve shows tolueneadsorption capacity of 18.22 mg/g and desorption efficiency about 77%, together with the maximum desorptiontemperature at 100 C. 3Pt1Pd/molecular sieve owns toluene conversion with 38.3% and 100% at100 and 200 C, accompanied with the higher CO2 selectivity of 0.0% and 150.0%, separately, due to thehigher Pd2+ content. The catalyst displays water vapor endurance, reproducibility, and 83.0% carbon balanceduring the whole adsorption–desorption–catalysis within 150 min, which highlights the sustainableapplicability in lean VOCs abatement. Furthermore, the possible catalytic reaction pathway of tolueneoxidation is proposed based on the in-situ DRIFTS experiments.

Genomic Correlates of Unfavorable Outcome in Locally Advanced Cervical Cancer Treated with Neoadjuvant Chemoradiation

Yuchun Wei,Chuqing Wei,Liang Chen,Ning Liu,Qiuxiang Ou,Jiani C. Yin,Jiaohui Pang,Zhenhao Fang,Xue Wu,Xiaonan Wang,Dianbin Mu,Yang Shao,Jinming Yu,Shuanghu Yuan 대한암학회 2022 Cancer Research and Treatment Vol.54 No.4

Purpose Neoadjuvant therapy modality can increase the operability rate and mitigate pathological risks in locally advanced cervical cancer, but treatment response varies widely. It remains unclear whether genetic alterations correlate with the response to neoadjuvant therapy and disease-free survival (DFS) in locally advanced cervical cancer. Materials and Methods A total of 62 locally advanced cervical cancer (stage IB-IIA) patients who received neoadjuvant chemoradiation plus radical hysterectomy were retrospectively analyzed. Patients’ tumor biopsy samples were comprehensively profiled using targeted next generation sequencing. Pathologic response to neoadjuvant treatment and DFS were evaluated against the association with genomic traits. Results Genetic alterations of PIK3CA were most frequent (37%), comparable to that of Caucasian populations from The Cancer Genome Atlas. The mutation frequency of genes including TERT, POLD1, NOS2, and FGFR3 was significantly higher in Chinese patients whereas RPTOR, EGFR, and TP53 were underrepresented in comparison to Caucasians. Germline mutations were identified in 21% (13/62) of the cohort and more than half (57%) had mutations in DNA damage repair genes, including BRCA1/2, TP53 and PALB2. Importantly, high tumor mutation burden, TP53 polymorphism (rs1042522), and KEAP1 mutations were found to be associated with poor pathologic response to neoadjuvant chemoradiation treatment. KEAP1 mutations, PIK3CA-SOX2 co-amplification, TERC copy number gain, and TYMS polymorphism correlated with an increased risk of disease relapse. Conclusion We report the genomic profile of locally advanced cervical cancer patients and the distinction between Asian and Caucasian cohorts. Our findings highlight genomic traits associated with unfavorable neoadjuvant chemoradiation response and a higher risk of early disease recurrence. PurposeNeoadjuvant therapy modality can increase the operability rate and mitigate pathological risks in locally advanced cervical cancer, but treatment response varies widely. It remains unclear whether genetic alterations correlate with the response to neoadjuvant therapy and disease-free survival (DFS) in locally advanced cervical cancer.Materials and MethodsA total of 62 locally advanced cervical cancer (stage IB-IIA) patients who received neoadjuvant chemoradiation plus radical hysterectomy were retrospectively analyzed. Patients’ tumor biopsy samples were comprehensively profiled using targeted next generation sequencing. Pathologic response to neoadjuvant treatment and DFS were evaluated against the association with genomic traits.ResultsGenetic alterations of <i>PIK3CA</i> were most frequent (37%), comparable to that of Caucasian populations from The Cancer Genome Atlas. The mutation frequency of genes including <i>TERT, POLD1, NOS2</i>, and <i>FGFR3</i> was significantly higher in Chinese patients whereas <i>RPTOR, EGFR</i>, and <i>TP53</i> were underrepresented in comparison to Caucasians. Germline mutations were identified in 21% (13/62) of the cohort and more than half (57%) had mutations in DNA damage repair genes, including <i>BRCA1/2, TP53</i> and <i>PALB2</i>. Importantly, high tumor mutation burden, <i>TP53</i> polymorphism (rs1042522), and <i>KEAP1</i> mutations were found to be associated with poor pathologic response to neoadjuvant chemoradiation treatment. <i>KEAP1</i> mutations, <i>PIK3CA-SOX2</i> co-amplification, <i>TERC</i> copy number gain, and <i>TYMS</i> polymorphism correlated with an increased risk of disease relapse.ConclusionWe report the genomic profile of locally advanced cervical cancer patients and the distinction between Asian and Caucasian cohorts. Our findings highlight genomic traits associated with unfavorable neoadjuvant chemoradiation response and a higher risk of early disease recurrence.

ZNF488 Enhances the Invasion and Tumorigenesis in Nasopharyngeal Carcinoma Via the Wnt Signaling Pathway Involving Epithelial Mesenchymal Transition

Dan Zong,Li Yin,Qian Zhong,Wen-jie Guo,Jian-hua Xu,Ning Jiang,Zhi-rui Lin,Man-zhi Li,Ping Han,Lin Xu,Xia He,Mu-sheng Zeng 대한암학회 2016 Cancer Research and Treatment Vol.48 No.1

Purpose The purpose of this study was to investigate the function of Zinc finger protein 488 (ZNF488) in nasopharyngeal carcinoma (NPC). Materials and Methods The endogenous expression of ZNF488 in NPC tissues, normal nasopharyngeal epithelium tissues and NPC cell lines were detected by quantitative reverse transcription polymerase chain reaction. ZNF488 over-expressing and knock-down NPC cell line models were estab- lished through retroviral vector pMSCV mediated over-expression and small interfering RNA (siRNA) mediated knock-down. The invasion and migration capacities were evaluated by wound healing and transwell invasion assays in ZNF488 over-expressing and control cell lines. Soft-agar colony formation and a xenograft experiment were performed to study tumorigenic ability in vitro and in vivo. Immunofluorescence and western blotting analysis were used to examine protein changes followed by ZNF488 over-expression. Microarray analysis was performed to explore gene expression profilings, while luciferase reporter assay to evaluate the transcriptive activity of Tcf/Lef. Results ZNF488 was over-expressed in NPC tissues compared with normal tissues, especially higher in 5-8F and S18, which are well-established high metastatic NPC clones. Functional studies indicate that over-expression of ZNF488 provokes invasion, whereas knock-down of ZNF488 alleviates invasive capability. Moreover, over-expression of ZNF488 promotes NPC tumor growth both in vitro and in vivo. Our data further show that over-expression of ZNF488 induces epithelial mesenchymal transition (EMT) by activating the WNT/β -catenin signaling pathway. Conclusion Our data strongly suggest that ZNF488 acts as an oncogene, promoting invasion and tumorigenesis by activating the Wnt/β -catenin pathway to induce EMT in NPC.

Seroprevalence of Toxoplasma gondii Infection in Police Dogs in Shenyang, Northeastern China

Cheng-Wu Liu,Na Yang,Jian-Bin He,Ming-Yang Mu,Min Yang,Ning Sun,Hong-Kui Li 대한기생충학열대의학회 2013 The Korean Journal of Parasitology Vol.51 No.5

A Method of Zero self-modification and Temperature Compensation for Indoor Air Quality Detection Based on a software Model

Cuiping Li,Jiuqiang Han,Qibin Huang,Xiaoqiang Dong,Xuequan Ding,Yaming Ding,Dianguo Zhang,Ning Mu 제어로봇시스템학회 2012 제어로봇시스템학회 국제학술대회 논문집 Vol.2012 No.10

It is very difficult to apply non-dispersive infrared sensor to detect the indoor air quality and maintain very low zero and temperature drift over long periods. Frequently manual zero setting and calibration are required. To solve the issues of zero and temperature drift of non-dispersive infrared sensor, a software model based on zero gas intensity, reference channels intensity, standard temperature, environmental temperature, temperature drift coefficient, etc. has been established to automatically modify and compensate the zero and temperature drift existing in the long-term continuous operation of the infrared sensor. The test result and long-term application indicate the detection precision of the instrument is less than 5%F.S in various changing environmental conditions. The average detection precision of carbon dioxide has been improved from 9.26% before comprehensive processing to 1.23% after processing, while the average detection precision of methane has been improved from 10.61% before comprehensive processing to 0.70% after processing. As a result, the disadvantages existing in many gas detectors including poor stability and short calibration cycle have been overcome, thus effectively improving the detection precision and stability of the instrument and reducing the maintenance cost.