Time-evolving genetic networks reveal a NAC troika that negatively regulates leaf senescence in <i>Arabidopsis</i>

Kim, Hyo Jung,Park, Ji-Hwan,Kim, Jingil,Kim, Jung Ju,Hong, Sunghyun,Kim, Jeongsik,Kim, Jin Hee,Woo, Hye Ryun,Hyeon, Changbong,Lim, Pyung Ok,Nam, Hong Gil,Hwang, Daehee National Academy of Sciences 2018 PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF Vol.115 No.21

<▼1><P><B>Significance</B></P><P>Leaf senescence is regulated in a complex manner, involving time-dependent interactions with developmental and environmental signals. Genetic screens have identified key regulators of senescence, particularly late-stage senescence regulators. Recently, time-course gene-expression and network analyses, mostly analyses of static networks, have predicted many senescence regulators. However, senescence is defined by time-evolving networks, involving the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks of NAM/ATAF/CUC (NAC) transcription factors, central regulators of leaf senescence in <I>Arabidopsis</I>, via time-course gene-expression analysis of NACs in their mutants. These time-evolving networks revealed a unique regulatory module of NACs that controls the timely induction of senescence-promoting processes at a presenescent stage of leaf aging.</P></▼1><▼2><P>Senescence is controlled by time-evolving networks that describe the temporal transition of interactions among senescence regulators. Here, we present time-evolving networks for NAM/ATAF/CUC (NAC) transcription factors in <I>Arabidopsis</I> during leaf aging. The most evident characteristic of these time-dependent networks was a shift from positive to negative regulation among NACs at a presenescent stage. ANAC017, ANAC082, and ANAC090, referred to as a “NAC troika,” govern the positive-to-negative regulatory shift. Knockout of the NAC troika accelerated senescence and the induction of other <I>NAC</I>s, whereas overexpression of the NAC troika had the opposite effects. Transcriptome and molecular analyses revealed shared suppression of senescence-promoting processes by the NAC troika, including salicylic acid (SA) and reactive oxygen species (ROS) responses, but with predominant regulation of SA and ROS responses by ANAC090 and ANAC017, respectively. Our time-evolving networks provide a unique regulatory module of presenescent repressors that direct the timely induction of senescence-promoting processes at the presenescent stage of leaf aging.</P></▼2>

초·중·고 학생들의 과학 정의적 특성 추이 분석을 위한 종단적 연구

김효남,정완호,정진우,양일호,김영신 한국교원대학교 과학교육연구소 1999 청람과학교육연구논총 Vol.9 No.1

이 연구는 우리 나라 학생들의 과학의 정의적 특성의 성취 수준을 종단적으로 분석하여 국가 수준에서 과학교육 성과를 가늠해 봄으로써, 과학교육의 질을 향상시킬 수 있는 과학교육 정책의 기초자료를 제공하는데 그 목적이 있다. 이를 위하여 1997년 2월에 초중고학생 3603명, 1999년 3월에 초중고학생 4737명을 대상으로 과학의 정의적 특성을 조사하였다. 본 연구의 결과는 학교급별 과학의 정의적 특성의 성취 수준이 초등학교에서 중학교까지 통계적으로 유의미하게(p<.05) 하락 한 후 고등학교에서 상승하는 것으로 나타났다. 또한, 남학생이 여학생보다 통계적으로 유의미하게 높게 나타났다(p<.05). 남녀간의 성취수준의 차이는 학년이 올라갈수록 감소하는 것으로 나타났다. 지역별로는 소도시보다 대도시가 높게 나타났으며, 시간이 지날수록 그 차이가 커지는 것으로 나타났다. 1997년과 1999년의 성취 수준을 비교해보면 1999년의 성취수준이 통계적으로 유의미하게 낮게 나타났다. 또한, 국가수준의 과학에 대한 성취도를 분석할 수 있는 모니터 과정이 계속적으로 이루어지길 기대한다. The purpose of this study was to longitudinally analyze Korean students' trend on science-related affective characteristics and provide descriptive data for improving the quality of Korean science education. In this longitudinal study, 3603 students and 4737 students were sampled in elementary and secondary schools, respectively in January 1997 and March 1998. The result showed that students' affective characteristics were decreased with statistical significance(p<0.05) during elementary and junior high school periods, but students' affective characteristics were increased during high school period. Affective characteristics of male were significantly higher than those of females' (p<0.05). Difference on affective characteristics between female and male students was being decreased by increasing grades. Students' affective characteristics in metropolitan areas were significantly higher than those in regional areas and difference between them was increased in 1999 comparing to 1997. The students' affective characteristics in 1999 were lower than those in 1997 totally.

문화기술적 방법에 의한 초등학교 3학년 학생의 식물 관찰능력 조사

김진영,김효남 한국교원대학교 과학교육연구소 2003 청람과학교육연구논총 Vol.13 No.1

This study was examine the 3rd graders to observe plants. So to speak, it was a survey of how much their capacity was, how many the items of observations were, how the types of observations were, and what the wrong case and the good case were. The researcher took part in their activities, took notes, recorded the scenes on video tape, and allowed the students to produce the observation report which served as a method of collecting the research data. After that, the researcher analyzed the data collected including the interview data with some students. The following research conclusions were: First, the third grades have the capacity to find some characteristics of plants observed. They understood what they should observe from each suggested plant and subsequently made a few observations appropriate for their academic level. Second, at the average number of frequency in plant observation per student, nonoperational observations were 3.5, operational observations, measurements, and observations of difference and similarity were 0.5. And there were a few of good case and bad case in the consequence of analysis. Nonoperational observations were centered for shape, color, feel, and smell, and the number of them was 7 times of the other items of observations, so we can say that they used mostly the five senses. As a result of analyzing the contents of students' reports faulty data had many abstract expressions, where as reports containing more complete and better analyzed data included more concrete and detailed expressions. Therefore, we can conclude that the third grades need more practice drills in observation ability.

자연과 수업에서 Vee Diagram 의 적용에 대한 연구

정진우,송보용,김효남 한국교원대학교 과학교육연구소 1994 청람과학교육연구논총 Vol.4 No.1

The purpose of this study is to analize the effect of Vee Diagram on the ability of scientific investigation, scientific attitude, cognitive ability in elementary school. For this study, 68 sixth grade students were selected and they were divided into two group: one is the experimenal group with Vee diagram instruction, the other is the control group with conventional instruction. Data were collected from the samples to know effect of Vee Diagram instruction and compared the experimental group with the control group. The result of analysis is as follows: 1. The vee diagram instruction is better than the conventional instruction for improving the scientific inquiry skills. 2. The vee diagram instruction does not show statistically meaningful difference to the conventional instruction for improving the scientific attitude. 3. The vee diagram instruction is better than conventional instuction for improving scientific knowledge. The results indicated that Vee diagram instruction is effective in learning strategy for improving scientific attitude.

국가수준의 과학에 관련된 정의적 특성의 평가체제 개발

김효남,정완호,정진우 한국교원대학교 과학교육연구소 1998 청람과학교육연구논총 Vol.8 No.1

학생들의 과학적 소양을 기르는데에는 과학과 관련된 정의적 특성이 중요한 역할을 한다. 과학적 태도, 자연과 과학에 대한 가치, 호기심과 관심 등의 과학과 관련된 정의적 특성이 대부분의 과학교육 목표에 포함되고 있다. 이 연구에서는 과학과 관련된 정의적 특성의 평가체제와 문항을 개발하였다. 인식, 흥미와 과학적 태도의 세 개의 주범주와 16개의 소범주로 평가체제가 구성되었다. 평가체제의 타당도는 10명의 과학교육학자들에 의하여 검증되었다. 48개의 평가 문항을 문항반응분석과 문항· 검사 상관계수를 고려하여 총 64 문항 중에서 선정하였다. 크론바하 알파계수는 인식과 흥미에서 0.83이고, 과학적 태도에서는 0.86이었다. 검사의 결과는 다음과 같다. 첫째, 과학과 관련된 정의적 특성에서 남학생이 여학생 보다 더 높은 점수를 나타내었다. 둘째, 초등학생이 중등학생보다 더 높은 과학과 관련된 특성 점수를 나타내었다. 마지막으로 도시지역의 학생이 시골지역의 학생보다 더 높은 과학과 관련된 특성 점수를 보여 주었다. Science-related affective domain is considered as a very important domain for achieving students' scientific literacy. Most science educational objectives include some objectives about affective domain such as scientific attitudes, values, curiosity, interests and concerns about nature and science. In this research, the evaluation framework and items of science-related affective domain were developed. The evaluation framework consists of three main categories such as cognition, interest and scientific attitudes and 16 subcategories. The validity of the evaluation framework is tested by ten science educators. Forty-eight items are selected among item pool by considering item response analysis and item-test correlation coefficient. The Cronbach α of the tests is 0.83(cognition and interest) and 0.86(scientific attitudes). The results of the test are as follows. First, boys got higher scores than girls. Second, elementary students got higher scores than secondary students. Finally, students in urban areas showed higher scores than those in rural areas.

맹출장애가 있는 하악대구치의 임상적 관리

손우성,이해경,남형진,강상욱,송효경,김성식 부산대학교 병원 암연구소 2009 부산대병원학술지 Vol.- No.25

초등학교 학생들의 귀납연역적 추론 능력과 정신 용량 및 보석 오류와의 관계

김설한,정진우,김효남 한국교원대학교 과학교육연구소 1998 청람과학교육연구논총 Vol.8 No.1

The purpose of this study was to analyze the problem solving strategies of elementary school students and to find out correlations between the functional mental capacity, the perseveration error and the Creature Card Task solving ability. To study this purpose, four categories were selected through pilot test. The sample consisted of 231, the 4th grade students and the 5th grade students in Inchon, Korea and selected 32 students among them. Three instruments were used in this study, Creature Card Task, FIT(Figural Intersection Test) and WCST(Wisconsin Card Sorting Test). Researcher interviewed 32 students about Creature Card Task solving strategies and tests with FIT, WCST. Major findings of the study are as follows: 1. Creature Card Task solving strategies of the selected 4th & 5th grade students were different. Some students solved problems during individual interviews. 2. Creature Card Task solving abilities were significantly correlated with the functional mental capacity and the perseveration error.

악골에 발생한 중심성 골종(Central Osteoma)의 치험례

김일규,김효정,최진호,장금수,오남식 대한악안면성형재건외과학회 2002 Maxillofacial Plastic Reconstructive Surgery Vol.24 No.1

Two cases are described of Endosteal Osteoma which developed in the Jaw bone and were painful. The tumors were radiographically characterized by dense radiopaque lesion. The surface was smooth and overlying mucosa was normal in color. Tentative diagnosis was OSteoma. The tumors were treated surgically. Postoperatively the patients made uneventful recovery and the wound healed satisfactory. Postoperative radiograph showed that radiopaque mass had been completely removed. Histologically, biopsy was reported as Central Osteoma. Subsequent follow-up examinations show no recurrence of the lesion or the pain.

자폐장애 환자에서 FMR-1 유전 삼염기 반복의 분자생물학적 분석

곽호순,정철호,전효진,장은진,김희철,김정범,박영남 대한소아청소년정신의학회 2000 소아청소년정신의학 Vol.11 No.1

연구목적 : 자폐장애의 원인을 유전학적으로 규명하려는 연구가 시도되고 있으며, 그 중 fragile-X 증후군과의 연관성에 대한 연구가 활발히 진행되고 있다. fragile-X 염색체(Xq27.3)는 세포유전학적 방법으로 증명할 수 있으나 검사에 많은 제약과 단점이 있으므로, 본 연구에서는 보다 신뢰성이 높은 분자 생물학적 방법으로 FMR-1 유전자내 CGG 삼염기 반복부위를 분석하여 자폐장애와 fragile-X 증후군의 연관성을 규명하고자 하였다. 방법 : 자폐장애 환아(99명)와 정상대조군(8명)을 대상으로 FMR-1 유전자의 CGG 반복배열 부위를 sense와 antisense primer를 이용하여 PCR법으로 분석하엿으며, 동시에 세포유전학적 검사도 시행하였다. PCR 분석에서 CGG 반복수가 50 이상인 경우에 대해서는 StB12.3 혹은 Pfxa3 probe를 이용한 Sourthern blot hybridization으로 확인하였다. 결과 : FMR-1 유전자의 CGG 반복배열에 대한 PCR 분석 결과 CGG 삼염기의 반복배열의 수는 자폐장애 환자군과 정상대조군 사이에 통계적으로 유의한 차이가 없었다(p=0.207). 자폐장애 환자에서 CGG 반복수가 50회 이상인 조기변이(premutation) 환자가 2명 있었으나 Sourthern blot hybridization 결과 완전변이(full mutation)로 판정할 수 있는 경우는 없었다. 세포유전학적 검사에서 환자군 모두에서 정상 핵형을 나타내었으며 fragile-X 염색체는 확인되지 않았다. 결론 : 이상의 결과에서 자페장애 환자가 FMR-1 유전자의 CGG 삼염기 반복부위 이상, 즉 fragile-X 염색체 이상을 동반하지 않았음을 증명할 수 있었다. 이는 fragile-X 증후군을 자�장애의 직접적인 원인이라고 보기에는 어려움이 있음을 시사한다. Objectives : There has been a rapid expansion of studies aimed at elucidating the genetic basis of autistic disorder, especially it's relationship to fragile-X syndrome. The detection of fragile X chromosome(Xq27.3) by cytogenetic analysis has revealed many difficulties in testing. Therefore, to explore the relationship between autistic disorder and fragile X syndrome, this study administered molecular biologic methods which examined an unstable CGG repeat within the fragile X mental retardation-1(FMR-1) gene. Methods : Ninety nine autistic children and eight normal control children were tested. The number of CGG repeats within FMR-1 gene was measured after amplification by PCR, and cytogenetic analysis was also carried out to detect fragile site Xq27.3. Sourthern blot hybridization, using StB12.3 and/or Pfxa3 probe, was done for the patients showing expansion of more than 50 CGG repeats(premutation). Results : All but two autistic patients had no expansion in CGG repeats by PCR and there was no significant statistical difference in number of CGG repeat in comparison with normal control. Two autistic patients, considered as premutation by PCR analysis, had no full mutation or premutation by Southern blot hybridization. All autistic children tested did not have any abnormal karyotype or fragile site Xq27.3. Conclusions : These results suggest that autistic patients may not have abnormality in FMR-1 gene or abnormal expansion in CGG repeat. In conclusion, fragile X syndrome may not be antecedent of autistic disorder. KEY WORDS : Autistic disorder·FMR-1 gene trinucleotide repeats·Molecular biologic analysis.

위암과 식도암이 병발된 다발성 원발성 악성종양 1예

박사영,이나영,이효진,이선영,최진혁,이순남,심강섭,성순희,한운섭 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

Multiple primary cancer means that more that two cancers occur independently in an individual. Recently, the incidence of multiple primary cancer has increased with lengthened survival, of cancer patients, development of new diagnostic technique and increased clinical evaluation. We report a patient who had adenocarcinoma of stomach combined with squamous cell carcinoma of esophagus simultaneously.