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Nakano, Takafumi,Seo, Hong-Yul The Korean Society of Systematic Zoology 2014 Animal Systematics, Evolution and Diversity Vol.30 No.2
Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, $tRNA^{Cys}$, $tRNA^{Met}$, 12S rRNA, $tRNA^{val}$, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.
Masuo Nakano,Shiro Nakano,Hideo Takekoshi 한국식품영양과학회 2007 Journal of medicinal food Vol.10 No.1
In addition to meeting nutritional requirements, breast milk plays important roles in biodefense for nursing in-fants. Dioxins have been detected at high concentrations in breast milk, raising concerns about disorders in nursing infantscaused by breast milk containing dioxins in Japan. We analyzed dioxin levels in breast milk and maternal blood samples from35 pregnant women in Japan. We also measured immunoglobulin (Ig) A concentrations in breast milk and investigated cor-relations with dioxin concentrations. In addition, 18 of the 35 women took Chlorella pyrenoidosa(Chlorella) supplementsduring pregnancy, and the effects on dioxin and IgA concentrations in breast milk were investigated. Toxic equivalents weresignificantly lower in the breast milk of women taking Chlorellatablets than in the Control group (P. .003). These resultssuggest that Chlorellasupplementation by the mother may reduce transfer of dioxins to the child through breast milk. No sig-nificant correlation was identified between dioxin and IgA concentrations in breast milk in the Control group. It is unlikelythat normal levels of dioxin exposure via food have a remarkable influence on IgA in breast milk. IgA concentrations in breastmilk in the Chlorellagroup were significantly higher than in the Control group (P. .03). Increasing IgA levels in breast milkis considered to be effective for reducing the risk of infection in nursing infants. The present results suggest that Chlorellasupplementation not only reduces dioxin levels in breast milk, but may also have beneficial effects on nursing infants by in-creasing IgA levels in breast milk.
Takafumi Nakano,Hong-Yul Seo 한국동물분류학회 2014 Animal Systematics, Evolution and Diversity Vol.30 No.2
Specimens of the genus Orobdella Oka, 1895 from Korea, including various locations in the Korean Peninsula, were identified as Orobdella tsushimensis Nakano, 2011. Phylogenetic analyses using mitochondrial cytochrome oxidase subunit 1 (COI), ND1, tRNACys, tRNAM<SUP>et</SUP>, 12S rRNA, tRNA<SUP>val</SUP>, and 16S rRNA markers show that the newly collected specimens form a monophyletic group with the known O. tsushimensis specimens. The genetic distance of COI of these specimens was in the range 0.4-6.6%. These results confirm that the newly collected specimens belong to O. tsushimensis. This is the first record of the genus Orobdella from the Korean Peninsula.
Akiyo Nakano,Ryuichi Nakano,Yuki Suzuki,Kyoichi Saito,Kei Kasahara,Shiro Endo,Hisakazu Yano 대한진단검사의학회 2018 Annals of Laboratory Medicine Vol.38 No.4
Dear Editor, Carbapenem-resistant Enterobacteriaceae have acquired carbapenemase genes [1], which differ substantially across countries [2]. Transferable carbapenemase IMP-type metallo-β-lactamases, particularly IMP-1 and IMP-6, are commonly identified in the clinical setting in Japan [3, 4] and exhibit different substrate specificity despite having a difference of only one amino acid (IMP-6: Ser214Gly). IMP-1 producers are more resistant to imipenem than to meropenem, whereas IMP-6 producers are more resistant to meropenem [5]. We previously found that the susceptibility rate of IMP-6-positive Escherichia coli was higher for imipenem than for meropenem [3]. Thus, IMP-6-producing isolates may be erroneously categorized as imipenem-susceptible, which could lead to treatment failure in patients.
Hong-Yul Seo,Takafumi Nakano 한국응용곤충학회 2012 한국응용곤충학회 학술대회논문집 Vol.2012 No.05
New record of family of arhynchobdellida leeches (Orobdellidae) from Gageodo Island is presented. We have surveyed for land leeches in Korea from July to September in 2011 and found 1 species. This is Orobdella tsushimensis Nakano, 2011, reported for the first time in Korean Peninsula. This species gets the following characteristics: IV unianulate; 1/2+5 annuli between gonopores; bottle-shaped gastroporal duct; and epididymides in XVII–XIX. According to their morphological features, the diagnosis of O. tsushimensis is slightly emended. Phylogenetic analyses using mitochondrial COI, tRNACys, tRNAMet, 12SrDNA, tRNAVal and 16SrDNA markers show that the Orobdella specimen from Gageodo Isl. and O. tsushimensis from Tsushima Island, Japan, form a monophyletic clade and also confirm the identification of the Orobdella specimens from Gageodo Isl.
Nakano, Keisuke,Yoshioka, Hiroshi,Sengoku, Masakazu,Shinoda, Shoji 대한전자공학회 1996 APCCAS:Asia Pacific Conference on Circuits And Sys Vol.1 No.1
Dynamic Channel Assignment (DCA), which improves the efficiency of channel use in cellular mobile communication systems, requires finding an available channel for a new call after the call origination. This causes the delay which is defined as the time elapsing between call origination and completion of the channel search. For system planning, it is important to evaluate the delay characteristic of DCA because the delay corresponds to the waiting time of a call and influences service quality. It is, however, difficult to theoretically analyze the delay characteristics except its worst case behavior. The objective of this paper is analyzing the distribution and the mean value of the delay theoretically. This analysis is based on the Clique Packing model for analyzing the blocking rate performance of DCA.
Nakano, Hideo,Okumura, Reiko,Goto, Chinatsu,Yamane, Tsuneo The Korean Society for Biotechnology and Bioengine 2002 Biotechnology and Bioprocess Engineering Vol.7 No.5
By the in vitro combinatorial mutagenesis, which is a sequential reaction of PCR mutagenesis and in vitro coupled transcription/translation with Escherichia coli S30 extract, S65 and E222 of green fluorescent protein of Aequarea victoria were comprehensively changed to all possible combinations of amino acids, thus totally 400 mutant (including a wild type) proteins were simultaneously produced and their fluorescent properties were analyzed. Although a few mutations had been reported so far at the 222nd position, replacement E222 to all other19 amino acids gave fluorescent signal to the mutants by changing Ser 65 to Ala together. Among the mutants, replacement to G, A, S, Q, H and C gave relatively high fluorescence. The in vitro combinatorial mutagenesis, therefore, has been proved valuable for comprehensive structure-function studies of proteins.
Nakano, Yoshiharu,Ishiwata, Tetsuya,Yamaguchi, Satoshi,Toki, Masanori,Igarashi, Minoru,Usui, Yoshiharu,Miyamae, Hiroshi,Yamada, Yoichi,Yasuda, Heinosuke Korean Society of Photoscience 1999 Journal of Photosciences Vol.6 No.4
Various arylidene- or alkylidenemalononitriles are condensed with electrophilic ethylenes to obtain polycyanoaniline derivatives. All the anilines showed strong fluorescence and the fluorescence intensities was evaluated to the effect of substituents.
Bioavailability of Iron-fortified Whey Protein Concentrate in Iron-deficient Rats
Nakano, Tomoki,Goto, Tomomi,Nakaji, Tarushige,Aoki, Takayoshi Asian Australasian Association of Animal Productio 2007 Animal Bioscience Vol.20 No.7
An iron-fortified whey protein concentrate (Fe-WPC) was prepared by addition of ferric chloride to concentrated whey. A large part of the iron in the Fe-WPC existed as complexes with proteins such as ${\beta}$-lactoglobulin. The bioavailability of iron from Fe-WPC was evaluated using iron-deficient rats, in comparison with heme iron. Rats were separated into a control group and an iron-deficiency group. Rats in the control group were given the standard diet containing ferrous sulfate as the source of iron throughout the experimental feeding period. Rats in the iron-deficiency group were made anemic by feeding on an Fe-deficient diet without any added iron for 3 wk. After the iron-deficiency period, the iron-deficiency group was separated into an Fe-WPC group and a heme iron group fed Fe-WPC and hemin as the sole source of iron, respectively. The hemoglobin content, iron content in liver, hemoglobin regeneration efficiency (HRE) and apparent iron absorption rate were examined when iron-deficient rats were fed either Fe-WPC or hemin as the sole source of iron for 20 d. Hemoglobin content was significantly higher in the rats fed the Fe-WPC diet than in rats fed the hemin diet. HRE in rats fed the Fe-WPC diet was significantly higher than in rats fed the hemin diet. The apparent iron absorption rate in rats fed the Fe-WPC diet tended to be higher than in rats fed the hemin diet (p = 0.054). The solubility of iron in the small intestine of rats at 2.5 h after ingestion of the Fe-WPC diet was approximately twice that of rats fed the hemin diet. These results indicated that the iron bioavailability of Fe-WPC was higher than that of hemin, which seemed due, in part, to the different iron solubility in the intestine.
Measurement of time-dependent CP asymmetries in B0→KS0ηγ decays
Nakano, H.,Ishikawa, A.,Sumisawa, K.,Yamamoto, H.,Adachi, I.,Aihara, H.,Al Said, S.,Asner, D. M.,Aulchenko, V.,Aushev, T.,Ayad, R.,Babu, V.,Badhrees, I.,Bansal, V.,Behera, P.,Beleñ,o, C.,Bhuyan, American Physical Society 2018 Physical Review D Vol.97 No.9
<P>We report a measurement of time-dependent CP violation parameters in B-0 -> K-S(0)eta gamma decays. The study is based on a data sample, containing 772 x 10(6)B(B)over-bar pairs, that was collected at the Upsilon(4S) resonance with the Belle detector at the KEKB asymmetric-energy e(+)e(-) collider. We obtain the CP violation parameters of S = -1.32 +/- 0.77(stat) +/- 0.36(syst) and A = -0.48 +/- 0.41(stat) f 0.07(syst) for the invariant mass of the K-S(0)eta system up to 2.1 GeV/c(2).</P>