인슐린 비의존형 당뇨병 환자에서 혈장 Endothelin-1농도의 변화

신양수,조희충,김원식,국기용,김용화,정종훈,문철웅,배학연,양성훈 朝鮮大學校 附設 醫學硏究所 1992 The Medical Journal of Chosun University Vol.17 No.2

Endothelin(ET) is a 21-residue peptide originally isolated from the cultured porcine endothelial cells. There are at least three genes for endothelin:endothelin-1(ET-1), endothelin-2(ET-2), and endothelin-3(ET-3). Endothelins are present in various human biological fluids including plasma, urine, breast milk, and saliva and have been found elevated plasma ET concentrations in patients with diabetes mellitus, Patients undergoing maintenance hemodialysis due to chronic renal failure, patients with acute myocardial infarction, and patients with subarachnoid hemorrhage. Endothelial cell damage is suspected to occur in diabetic patients and may be one important cause of angiopathy, a major complication in diabetes mellitus. The elevation of ET in diabetic patients may be a marker of, and further exacerbate, their vascular disease. We measured the levels of ET-1 in plasma of 50 patients with non-insulin dependent diabetes mellitus(NIDDM) and 25 normal subjects by radioimmunoassay. The plasma ET-1 concentration (mean±S.D.) in NIDDM was 6.461 A2.510 f㏖/ ㎖, and was significantly higher than in normal subjects (4.567±1.155f㏖/㎖) (P<0.05). The plasma ET-1 concentration (mean±S.D.) in diabetic retinopathy group( 7.15±2.454 f㏖/ml) was significantly elevated than those in otherwise uncomplicated groups (5.348±2.390 f㏖/㎖)(P<0.01). The correlation between any clinical parameters and plasma ET-1 levels in NIDDM was not significant, In conclusion, this study suggest that the elevated levels of ET-1 in diabetic patients may be play a important role in the pathogenesis of diabetic complications.

Eethyl benezylidencyanoacetate 유도체에 대한 thiophenol 첨가 반응의 속도론적 연구

맹주양,黃善岩,金文執,崔元植,朴茂淳 順天鄕大學 1991 순천향대학논문집 Vol.14 No.1

Spermine에 의한 흰쥐 복강 비만세포의 히스타민 유리와 Camp, Cgmp 수준변동에 미치는 상백피의 효과

채옥희,양문식,이무삼 大韓免疫學會 1996 大韓免疫學會誌 Vol.18 No.3

Cortex Mori(Morus alba L.), the root bark of mulberry tree, has been used as an antiphlogistic, diuretic, and expectorant in Korean herbal medicine. Previous studies demonstrated that phenolic extract of Cortex Mori has a hypotensive effect in rabbits and hot water extract of Cortex Mori has an inhibitory effects on spermine and compound 48/80-induced mast cell degranulation and histamine release from rat peritoneal mast cells(RPMC). The purpose of this study is to determine whether Cortex Mori could affect the intracellular levels of cAMP and cGMP of RPMC by spermine which has been known as activators to increase cytoplasmic Ca" concentration by stimulating transmembrane Cat+ transport and mobilizing intracellular calcium, and release histamine from RPMC ultimately. Morphological changes of RPMC by spermine alone or Cortex Mori plus spermine were observed on the inverted light microscope. Various doses of spermine(10, 5, 1, 0.5, 0.1mM) were added into RPMC suspension (106cells/ml) pretreated with or without various doses of Cortex Mori(100, 50, 10, 5, lmg/ml) at 37°C for 10 minutes. After incubation, the amount of hitamine in supernatant, intracellular cAMP, and cGMP levels of RPMC were measured by radioisotope enzymatic histamine assay, cAMP and cGMP radioimmunoassays. Results were 1) the spermine(lOmM)-induced degranulation and histamine release from RPMC pretreated with Cortex Mori were significantly inhibited, compared to those of control without Cortex Mori pretreatment, 2) the cAMP and cGMP levels of unstimulated RPMC were 17.5 ± 1.9 and 120 x 10-3± 20 pmols, respectively, 3) after the addition of the spermine, the intracellular levels of cAMP were decreased and those of cGMP were increased, 4) dose dependent fashions of cAMP and cGMP levels of RPMC were induced by increased concentrations of spermine, 5) Cortex Mori increased the cAMP levels(200.0± 11.4%) and decreased the cGMP levels (22.5±4.2%) of RPMC remarkably, compared to those of normal control, and 6) the spermine-induced cAMP levels of RPMC pretreated with Cortex Mori weresignificantly increased, compared to those of positive control without Cortex Mori pretreatment and the spermine-induced cGMP levels of RPMC pretreated with Cortex Mori were remarkably decreased, compared to those of positive control without Cortex Mori pretreatment. From the above results, it is suggested that Cortex Mori contains some substances to inhibit the spermineinduced mast cell activation so as mast cell degranulation, histamine release, and that its inhibitory effect may occure via specific mechanisms of increasing of intracellular cAMP levels or blocking of increasing of intracellular cGMP levels.

대장균 세포내 칼모듈린 돌연변이체 고발현을 위한 클로닝 및 Chemiluminescence 검출법에 의한 발현분석

오석홍,박윤식,양문식,최동성 우석대학교 자연과학연구소 1996 自然科學硏究論文集 Vol.1 No.-

To obtain a large amount of VU-4 calmodulin (115, Lys to Ile). VU-4 calmodulin gene with Ptac promoter (680 bp) was cloned into a plasmid, pUC18. Newly cloned pUC18/VU-4 was transferred into E. coli UT481. The plasmid isolated from cell extracts of the transformed E. coli was analyzed by restriction mapping and yielded the expected restriction endonuclease map with BamHI and EcoRI. Calmodulin was detected by Western blot analysis using chemiluminescence protocol and the estimated expression level was 60 pmole calmodulin/mg protein. Calmodulin was purified from the E. coli cell extracts by Ca^(2+)-dependent chromatography on a phenyl-Sepharose column. The purified calmodulin was identified as a mutant VU-4 calmodulin by SDS-PAGE and methyltransferase substrate test.

點播粒數에 따른 靑刈大豆의 生育反應 및 飼料成分變化

趙南棋,吳章植,朴良門,宋昌吉 濟州大學校亞熱帶農業硏究所 1997 亞熱帶農業硏究 Vol.14 No.-

本 硏究는 濟州道에 있어서 點播粒數에 따른 濟州在來大豆의 生育反應 및 飼料成分에 미치는 影響을 究明하기 위하여 1996年 5月 10日부터 8月 16日까지 遂行하였던 結果를 要約하면 다음과 같다. 1. 草長은 4粒區에서 115cm로 가장 길었으며, 1粒區에서 110cm로 가장 짧았다(Y=-0.527X^2+3.885X+107.110). 2. 莖直徑, 分枝數, 葉數 等의 形質은 點播粒數가 많아질수록 減少되는 傾向이었다. 3. 生草收量은 4粒區에서 10a當 3633kg으로 가장 많았으며, 3粒과 5粒區는 中間이었고, 1粒區에서는 2349kg으로 가장 적었다.(Y^*=-125.088X^2+995.387X+1510.790) 4. 乾草收量은 4粒區에서 839kg으로 가장 많았으며, 5粒과 6粒區에서는 中間이었고, 1粒區에서는 509kg으로 가장 적은 편이었다.(Y^*=-23.839X^2+221.255X+279.720) 5. 組蛋白賃과 粗脂肪은 點播粒數가 많아질 수록 增加하였으며, 粗纖維와 粗灰分은 減少되었다. 6. 草長은 生草收量과 正의 相關을 나타내었고, 莖直徑·分枝數·葉數·葉重 等의 形質과는 高度로 有意한 正의 相關을 나타냈으며, 粗焉白質과는 負의 相關을 나타냈다. The study has been conducted to clarify the effects of number of seeds per hill in dibbling on the growth and feed composition of Cheju Local Soybean from May 10 to August 16 in Cheju-Do. The results obtained are summarized as follows ; 1. Plant height when four seeds per hill in dibbling(115cm) was the longest, while one seeds per hill in dibbling(110cm)was the shortest(Y=-0.527X^2+3.885X+107.110). 2. The diameter of stems, number of branches and number of leaves per plant decreased with the number of seeds per hill in dibbling increased. 3. Fresh yield per 10a when four seeds per hill in dibbling(3633kg) were the greatest and three seeds per hill in dibbling, when five seeds per hill in dibbling were intermediate but one seeds per hill in dibbling(2349kg) was the lightest. (Y^*=-125.088X^2+995.387X+1510.790) 4. Dry yield per 10a when four seeds per hill in dibbling(839kg) were the heaviest and when five, six seeds per hill in dibbling were intermediate but one seeds per hill in dibbling(509kg) was the lightest(Y^*=-23.839X^2+221.255X+279.720). 5. Crude protein and crude fat increased with the number of seeds per hill in dibbling increased, but crude fiber and crude ash decreased. 6. There was positive correlation between plant height and fresh yield, and further highly positive correlation between plant height and the diameter of stems, number of branches, number of leaves, or the weight of loaves and negative correlation between plant height and crude protein.

Eethyl benezylidencyanoacetate 유도체에 대한 thiophenol 첨가 반응의 속도론적 연구

朴茂淳,金文執,崔元植,黃善岩,孟柱煬 순천향대학교 1991 논문집 Vol.14 No.1

Reaction of benzaldehyde derivatives with ethylacetate afforded ethylbenzylidenecyanoacetate. Especially, the crystal structure of ethyl p-methoxybenzylidenecyanoacetate were demonstrated by X-ray diffrection method. The crystal structure is only exist in E-form. The kinetic studies on the mechanism of nucleophilic addition of thiophenol to ethyl benzylidenecyanoacetate have been investigated by UV-spectrophotomery and the rate equation which can be applied over wide pH range was obtained. From the rate equation, effect of generalbase and substituent, the fellowing reaction proposed. Below pH 3.0, thiophenol added to the double bond, while above pH9.0 thiophenoxide ion added to the double bond. In the range of pH from 3.0 to 9.0, these reaction occurred competitively.

Wolff-Parkinson-White증후군의 임상상 및 전기생리학적 소견

안신기,이문형,장양수,오동진,최인석,하종원,임세중,김병옥,정남식,심원흠,조승연,김성순 대한순환기학회 1997 Korean Circulation Journal Vol.27 No.9

성인원외폐렴의 원인미생물에대한 전향적 다기관 연구 : 성인원외폐렴의 원인으로 세균의 역할을 중심으로

우준희,강재명,김양수,신완식,류진홍,최정현,김양리,정희진,어수택,박춘식,정문현,정기석,이찬주,류지소 대한감염학회 2001 감염 Vol.33 No.1

Background : Communite-acquired peumonia (CAP) is one of the leading causes of mortality and morbidity. Despite progress in diagnostic techniques and treatments, management of pneumonia remains challenging, because the precise etiology remains uncertain in as many as 49% of cases. The limitaions of identifying etiologic agents make it necessary to use empiric antibiotics in almost all patients, and furthermore emergence of antibiotic-resistant organisms pose difficulties to the selection of an empiric antibiotic regimen. For the optimal choice of empirical antibiotics, we should know the frequency of etiologic agents and antimicrobial resistance rates in the community. Methods : A prospective multicenter study of community-acquired pneumonia in Korea was carried out between May 1997 and April 2000. The microbiologic diagnosis was based on the results of sputum culture, blood culture and pleural culture. Results : Five hundred eighty eight cases of community-acquired peumonia in 562 patients admitted to the hospitals. The mean age was 59.9 with male predominance (58.3%), and 370 (63%) had underlyin gillness. The etiologic agents were identified in 38.3%, and the list of individual agents, in decreasing order, was Streprococcus pneumoniae (21.7%), Klebsiella pneumoniae (14.8%) Pseudomonas aeruginosa (9.8%), Staphylococcus aureus (9.5%), viridans group streptococci (5.7%), Enterobacter cloacae (4.2%), Hemophillus Influenza (3.8%), The rates of admission to the intensive care unit was 10.4%. The motality was 7.1%. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Forty percents of S. aureus were methicillin-resistant S. aureus. K. penumoniae were susceptible to cephalosporin and quinolone. Conclusion : In Korea, S.pneumoniae is the most important agent causing community-acquired pneumonia. Susceptible rates of S. pneumoniae to penicillin was 36.6% and showed multidrug resistant. Gram negative bacteria such as K. pneumoniae, P. aeruginosa showed high incidence when compared with that of other countries. Most of them had underlying diseases including bronchiectasis and chronic obstructive pulmonary diseases. (Korean J Infect Dis 33:1∼7, 2001)

Herbicide Resistance of Tobacco Chloroplasts Expressing the bar Gene

Moon-Sik Yang,Tae-Jin Kang,서조은,Nguyen-Hoang Loc 한국분자세포생물학회 2003 Molecules and cells Vol.16 No.1

Transgenic Tobacco Plants Expressing a Mutant VU-4 Calmodulin Have Altered Nicotinamide Co-Enzyme Levels and Hydrogen Peroxide Levels

Yang, Moon Sik,Oh, Suk Heung,Park, Yoon Sick 생화학분자생물학회 2000 BMB Reports Vol.32 No.1

In order to understand the biological role of calmodulin in plants, transgenic tobacco plants expressing a calmodulin mutant (VU-4 calmodulin, lys to ile-115) gene have been analyzed. SDS-PAGE and Western-blot analyses showed that the foreign calmodulin mutant is stably and highly expressed in the transgenic tobacco plants. The levels of H202 were elevated approximately 2-fold in the transgenic plants. Furthermore, the transgenic tobacco plants have more than 6-fold higher levels of NADPH compared to control tobacco plants. The present findings, combined with previous data showing differences in the susceptibility of the transgenic tobacco seeds and normal tobacco seeds to fungal contamination (Oh and Yang, 1996), suggest that the expression of the calmodulin derivative gene in tobacco plants could increase resistance to infection by fungal pathogens.