Effects of Lactobacillus salivarius Ren on cancer prevention and intestinal microbiota in 1, 2-dimethylhydrazine-induced rat model

Ming Zhang,Xing Fan,Bing Fang,Chengzhen Zhu,Jun Zhu,Fazheng Ren 한국미생물학회 2015 The journal of microbiology Vol.53 No.6

Probiotics have been suggested as a prophylactic measure in colon cancer. The aim of this study was to investigate the impact of Lactobacillus salivarius Ren (Ren) in modulating colonic microbiota structure and colon cancer incidence in a rat model after injection with 1,2-dimethyl hydrazine (DMH). The results indicated that oral administration of Ren could effectively suppress DMH-induced colonic carcinogenesis. A significant decrease in cancer incidence (87.5% to 25%) was detected in rats fed with a dose of 5 × 1010 CFU/kg bodyweight per day. Using denaturing gradient gel electrophoresis and Real-time PCR combined with multivariate statistical methods, we demonstrated that injection with DMH significantly altered the rat gut microbiota, while Ren counteracted these DMH-induced adverse effects and promoted reversion of the gut microbiota close to the healthy state. Tvalue biplots followed by band sequencing identified 21 bacterial strains as critical variables affected by DMH and Ren. Injection of DMH significantly increased the amount of Ruminococcus species (sp.) and Clostridiales bacteria, as well as decreasing the Prevotella sp. Administration of Ren reduced the amount of Ruminococcus sp., Clostridiales bacteria, and Bacteroides dorei, and increased the amount of Prevotella. Real-time PCR results were consistent with the results derived by t-value biplots. These findings suggested that Ren is a potential agent for colon cancer prevention. In conclusion, the results in the present study suggest a potential therapeutic approach based on the modulation of intestinal microflora by probiotics may be beneficial in the prevention of colorectal carcinogenesis.

뇌졸중환자의 인지기능에 관한 연구

류명인,소희영 충남대학교 간호과학연구소 2006 충남대 간호학술지 Vol.9 No.1

Purpose: This study was to explore the cognitive function of stroke patients. Method: The study sample were 88 who were possible to communicate and agreed to participate. The data was collected at OPD and wards from 2 Veteran's hospital located at Daejeon and Seoul and one C University hospital by trained nursing students at 2004 February. this research design is survey research. The measurement tool was MMSE-K. The data were analysed by SPSS 10.0 using frequency, t-test ANOVA, Pearson's correlation coefficients and Regression. Result: The mean score of MMSE-K was 26.74, being from minimum 13 to maximum 30. There was significant difference in attention/calculation subtype of MMSE-K according to length of education. There were statistically significant correlation among Time orientation, Place orientation, Registration, Attention & Calculation, Recall and Language. Conclusion: The cognitive function score of stroke patients was within normal limit. The length of education was a significant demographic characteristic affecting cognitive function. For rehabilitative care of stroke patient, the nurse has to collect data about cognition as well as demographic characteristics like income and perceived health status in order tar understand better and improve patients cognitive function.

Lactobacillus salivarius REN Counteracted Unfavorable 4-Nitroquinoline-1-Oxide-Induced Changes in Colonic Microflora of Rats

Ming Zhang,Xuewei Qiao,Liang Zhao,Lu Jiang,Fazheng Ren 한국미생물학회 2011 The journal of microbiology Vol.49 No.6

Probiotics and carcinogens both have a significant effect on the microfloral composition of the human intestine. The objective of this study was to investigate the impact of an important carcinogen, 4-Nitroquinoline-1- Oxide on colonic microflora and the efficacy of the probiotic Lactobacillus salivarius REN as an agent of counteracting these effects. Using denaturing gradient gel electrophoresis (DGGE) combined with redundancy analysis, we demonstrated that both 4-Nitroquinoline-1-Oxide and L. salivarius REN significantly altered the bacterial communities of rat colons. A total of 27 bacterial strains were identified as being affected by treatment with 4-Nitroquinoline-1-Oxide or L. salivarius REN using a t-value biplot combined with band sequencing. 4-Nitroquinoline-1-Oxide treatment increased the abundance of two potential pathogens (one Helicobacter strain and one Desulfovibrio strain), as well as reducing the abundance of two potentially beneficial strains (one Ruminococcaceae strain and one Rumen bacteria). The Helicobacter strain was initally detected in carcinogen-treated rat intestinal microflora, but L. salivarius REN treatment effectively suppressed the growth of the Helicobacter strain. These results suggested that L. salivarius REN may be a potential probiotic, efficiently acting against the initial infection with, and the growth of pathogenic bacteria.

IBM Zigbee Positioning Method for Smart Home Applications

Ming-Hui Jin,Chung-Jung Fu,Chih-Hao Yu,Hung-Ren Lai,Ming-Whei Feng 보안공학연구지원센터 2008 International Journal of Smart Home Vol.2 No.2

This paper presents a smart home system prototype which employs an indoor positioning system called the III Beacon Match (I3BM) positioning method to intelligently trigger the appropriately services for the home members. To overcome the ninja problem in the I3BM method, the signal filtering, adjustment and smooth procedures are proposed. The proposed system prototype employs the Zigbee module to implement the prototypes of the components for the I3BM positioning method, and the prototypes had passed the ZigBee Compliant Platform (ZCP) certification test. The proposed system prototype which intelligently controls the air condition and light system for smart home applications is also verifying in our demo room and in the smart house in National Taiwan University.

A 4th-Order Wideband Sigma Delta Modulator Using Linear System Theory Technique

Ming-yuan Ren,Yong-sheng Zhang,Wei-kun Wu,Zhen-xi Bao,Zi-wei Zhao,Chao Li,Hong-guo Zhang 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.1

A 4th-Order Wideband Sigma Delta modulator structure is proposed in this paper, which uses linear system theory technique. There are many implementation techniques to improve the performance of Sigma Delta ADCs, such as noise coupling, differential sampling and dynamic elements matching. An extra loop delay is needed to be added in the system. This paper explains how to make the modulator stable with the addition of feed-forward and feed-back branches which are used to compensate the extra loop delay. Next, we restore the signal and noise transfer functions. A 4th-Order Wideband delta-sigma modulator have been designed and simulated to verify the method.

Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue

Jiang, Ming Feng,Hu, Ming Jun,Ren, Hong Hui,Wang, Li Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.12

Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp open reading frames, which encodes 148 amino acids (16.54 kDa) with a signal peptide of 18 amino acids, was sequenced. Further analysis indicated that the nucleic acid and amino acid sequences identities between yak and cow milk lysozyme were 89.04% and 80.41%, respectively. Recombinant yak milk lysozyme (rYML) was produced by Escherichia coli BL21 and Pichia pastoris X33. The highest lysozyme activity was detected for heterologous protein rYML5 (M = 1,864.24 U/mg, SD = 25.75) which was expressed in P. pastoris with expression vector $pPICZ{\alpha}A$ and it clearly inhibited growth of Staphylococcus aureus. Result of the YML gene expression using quantitative polymerase chain reaction showed that the YML gene was up-regulated to maximum at 30 day postpartum, that is, comparatively high YML can be found in initial milk production. The phylogenetic tree indicated that the amino acid sequence was similar to cow kidney lysozyme, which implied that the YML may have diverged from a different ancestor gene such as cow mammary glands. In our study, we suggest that YML be a new c-type lysozyme expressed in yak mammary glands that plays a role as host immunity.

『북학의(北學議)』와 『설수외사(雪岫外史)』의 비교 연구

任明杰 ( Ren Ming-jie ) 성균관대학교 대동문화연구원 2019 大東文化硏究 Vol.108 No.-

본고는 연암 그룹의 중요한 구성원이며 북학파 중에서도 중국을 가장 많이 다녀온 지우로 楚亭 朴齊家(1750~1805)와 綸菴 李喜經(1745~1805 이후)의 연행체험을 통해 확인된 북학의 주장과 이용후생의 사상을 구체적으로 드러낸 『北學議』와 『雪岫外史』를 구체적으로 비교하였다. 두 사람 모두 서얼 출신이라는 면에서, 그리고 조선 후기 실학파 중의 이용후생학파의 중요한 구성원이란 점에서 이 두 책은 북학의 사상과 주장에서 많은 공통점을 드러낸다. 그러면서도 초정의 『북학의』와 윤암의 『설수외사』는 또한 각자의 특색을 분명하게 띠고 있다. 초정의 경우는 전체 사회의 제반 문제점에 관심을 두고 경세제민의 내용이 포괄하는 士農工商 전체에 대한 개혁을 주장하였는바 농업은 물론이고 공·상업, 유통업, 해외 통상, 사회제도에 이르기까지 풍부한 내용을 담고 있다. 윤암의 경우는 士農工商 중에서 ‘農’에 유독 관심을 두고 농업에 직접 관련된 분야로 확대되는 것으로 볼 수 있다. 『설수외사』는 『북학의』보다 후에 이루어진 저서이지만 오히려 상대적으로 보수적인 면모를 보이는데 이는 19세기 벽두 정조의 사망과 더불어 직면한 북학파의 위험한 입지 및 상황과 밀접한 관계가 있다. 이런 점에서 본다면 윤암의 『설수외사』는 북학을 주장한 연암그룹의 마지막 저서라고 할 수 있다. This thesis did comparison between Park Je-ga's Puk'agŭi and Lee, Hee-kyung's Solsuoesa, which specificly reflected the propositions of Bukhak School and the idea of utility and welfare confirmed by their Yeonhaeng experience. Park Je-ga(1750~1805) and Lee, Hee-kyung were good friends. Both of them were the important members of Yeonam group, who visited China most frequently. The two people went to China several times although they were both plebeians. Based on their Yeonhaeng experience, they expressed the thought of utility and welfare which was shared within Yeonam group by their writings, such as Puk'agŭi and Solsuoesa. As they were plebeians and important Silhak scholars with the consideration of utility and welfare, the two works revealed many common ideas and propositions of Bukhak. Meanwhile they clearly had their own characteristics on the contents. Through Puk'agŭi, we can knew that Park Je-ga cared about all the social problems and insisted on the comprehensive reform of scholar, farmer, artisan and merchant stratum, which was related with agriculture, industry and commerce, distribution industry, overseas trade and social system in order to administrate the country and relieve the people's suffering. Compared with him, Lee, Hee-kyung focused on the 'farmer' and the field directly related to agriculture. Solsuoesa was a later book than Puk'agŭi but rather conservative, which was closely related with the death of Jeongjo of Choson in the 19th century and the dangerous position and situation of Bukhak School. For this reason, Lee, Hee-kyung's Solsuoesa can be considered as the last work that advocated Bukhak of Bukhak School. In the future, the study on Yeonhaengroks of Bukhak scholars needs to pay attention on the differences of their works as well as the common points, so as to understand Bukhak School with a broader and more objective approach.

2000년대 북한의 대중 경제의존 및 영향분석

런밍 ( Ming Ren ) 조선대학교 동북아연구소(구 통일문제연구소) 2011 동북아연구 Vol.26 No.2

이 논문은 2000년대 북한의 대중경제 경제의존과 영향력을 분석한 것으로 그 내용을 몇 가지로 함축할 수 있다. 첫째, 북한은 현 시기 지역경제 블럭화, 단일화라는 세계경제의 조류와는 별개로, 동북아 지역에서 유일하게 사회주의 계획경제체제를 고수하고 있는 나라로서, 기본 경제발전전략인 ‘자립적 민족경제건설노선’을 확고부동하게 견지하고 있다. 따라서 대외무역이 경제발전 전반에서 차지하는 비중과 역할은 습유보결(拾遺補缺,또는 호통유무(互通有无)의 정도인 관계로 대외 경제의존도는 극히 낮은 편인데, 이는 또한 향후 상당기간 동안 북한경제는 세계경제, 특히 동북아 지역경제의 변화에 그 다지 큰 영향을 받지 않을 것으로 전망하게 한다. 하지만 북한의 제2차 핵실험 이후, 미국의 대북한 경제제재가 가속화되고 한국 이명박 정부가 ‘강한 한국’을 주창하며 북한에 대한 지원을 중단하면서, ‘믿을 만한 나라는 그래도 중국밖에 없다’는 인식을 바탕으로 중국에 대한 경제의존도가 매우 높아졌음을 발견할 수 있다. 특히 전략물자와 원조차원의 심각한 대중국 경제의존 현상을 포착할 수 있다. 둘째, 북한은 아직까지 대외무역 다각화를 실현하지 못해 무역대상국이 주로 중국, 한국, 일본, 러시아, 태국 등 극소수 국가에만 국한되어 있고, 특히 최근 중국, 한국에 대한 무역의존도가 약 80~90%를 차지하고 있다. 따라서 향후 중국과 한국의 대북한 경제정책의 변화는 북한의 대외 경제활동에 결정적인 영향을 미칠 것이며, 아울러 북한의 국내경제 회복과 성장·발전에도 중요한 역할을 할 것으로 예상된다. 셋째, 최근 북한은 철강, 원유, 식량, 기계, 의약품, 일용품 등 전략물자와 관련하여 심각한 대중국 의존현상을 보이고 있는데, 이러한 상황은 향후 상당기간 동안 지속될 것으로 예측된다. 넷째, 20세기 90년대 중·후반 북한경제는 붕괴의 위기를 겪었고, 외부의 원조를 통해 간신히 파국을 모면할 수 있었다. 21세기에 접어들면서 북한경제가 미약하나마 회복 성장을 시현해 가고 있지만, 최하 수준의 생계유지를 위해 여전히 국제사회의 원조에 의존하고 있으며, 그 중 중국과 한국의 지원이 절대적인 역할을 하고 있다. 특히 중국의 대 북한 식량 및 원유 원조는 북한의 생명 줄을 쥐고 있다고 해도 무방할 것이다. 다섯째, 최근 들어 북한이 2002년 ‘7.1 경제관리개선조치’에 비해 더욱 역행하는, 즉, 더욱 강화된 계획경제 조치들을 강구하고 있는 상황임을 감안할 때, 북한은 여전히 개혁·개방에 대해 폐쇄주의적 자세를 견지하고 있는 것으로 보인다. 결국, 북한이 단시일 내에 본격적인 대내개혁, 대외개방을 실시하고, 국제사회의 일원으로 등장하기는 어려울 것이며, 이로 인해 대중국 경제의존 현상은 지속 심화될 것으로 판단된다. 여섯째, 21세기로 진입하면서, 특히 한국의 이명박 정부가 출범하면서 중 북 경제관계는 역사상 그 어느 시점과 비교하여도 무역, 투자 및 기타 분야에서 양적으로나 질적으로 괄목할만한 발전을 가져왔으며 이러한 추세는 향후 수 년간 한층 더 업그레이드될 전망이다. This study analysed the dependence of the basic economy in North Korea in the first decade of the 2000s and the effects of this dependance. The results had a few implications. First, North Korea has adhered to a planned economic system of socialism only in the northeast area regardless of trends in the world economy, such as the formation of blocks and unification, and has firmly adhered to an independent national economy building policy. Therefore, as the weight and role external trade occupies in economic development was supplementary, the North`s dependence on external economic situations is extremely low. This indicates that North Korea`s economy, in particular the northeast economy, will not be greatly affected by the external world economy. However, since the second nuclear test in North Korea, American economic restrictions on North Korea have increased. Also as the Lee Government of South Korea has stopped support for North Korea, arguing against a nuclear armed North Korea, its dependence on China has increased. This is based on the recognition that North Korea can depend on China. In particular, the North`s dependence on China for strategic goods and support is acute. Second, North Korea has not realized the formation of multi-faceted external trade. It has had trade only with China, South Korea, Japan, Russia, and Thailand. In particular, the North`s trade dependence on China and South Korea amounts to between 80 and 90% of its external trade. Because of this, it is anticipated that changes in economic policy towards North Korea by China and South Korea will affect its external economic activities and, will play an important role in the recovery, growth, and development of the internal economy of North Korea. Third, North Korea has depended heavily on China for strategic goods such as steel, crude oil, food, machinery, medical supplies, and daily necessities. This dependance is expected to continue for the forseeable future. Fourth, North Korea risked a collapsing economy in the mid and late 1990s and narrowly avoided collapse through external support. In the 21st century, it has realized its recovery, but it is still dependant on support from international society in order to maintain minimum sustainability. For this, support from South Korea and China is absolutely necessary. In particular, it can be said that China`s support for North Korea in food and crude oil decides the life of North Korea. Fifth, in considering that North Korea has been pursuing more reinforced planned economic measures, which are opposite the ``7.1 Economic Control Measures`` published in 2002, it seems that North Korea is still adhering to a closed policy against innovation and opening. In conclusion, it is difficult for North Korea to execute the internal innovation or external opening necessary to appear as a member of international society, and therefore, its economic dependence will be maintained or intensified. Sixth, in the 21st century, in particular as the Lee Government launched, economic relations between North Korea and China have developed significantly in quality and quantity, and this trend will continue.

2000년대 북한의 대중 경제의존 및 영향분석

런밍 ( Ming Ren ) 朝鮮大學校 統一問題硏究所 2011 統一 問題 硏究 Vol.26 No.2

이 논문은 2000년대 북한의 대중경제 경제의존과 영향력을 분석한 것으로 그 내용을 몇 가지로 함축할 수 있다. 첫째, 북한은 현 시기 지역경제 블럭화, 단일화라는 세계경제의 조류와는 별개로, 동북아 지역에서 유일하게 사회주의 계획경제체제를 고수하고 있는 나라로서, 기본 경제발전전략인 ‘자립적 민족경제건설노선’을 확고부동하게 견지하고 있다. 따라서 대외무역이 경제발전 전반에서 차지하는 비중과 역할은 습유보결(拾遺補缺,또는 호통유무(互通有无)의 정도인 관계로 대외 경제의존도는 극히 낮은 편인데, 이는 또한 향후 상당기간 동안 북한경제는 세계경제, 특히 동북아 지역경제의 변화에 그 다지 큰 영향을 받지 않을 것으로 전망하게 한다. 하지만 북한의 제2차 핵실험 이후, 미국의 대북한 경제제재가 가속화되고 한국 이명박 정부가 ‘강한 한국’을 주창하며 북한에 대한 지원을 중단하면서, ‘믿을 만한 나라는 그래도 중국밖에 없다’는 인식을 바탕으로 중국에 대한 경제의존도가 매우 높아졌음을 발견할 수 있다. 특히 전략물자와 원조차원의 심각한 대중국 경제의존 현상을 포착할 수 있다. 둘째, 북한은 아직까지 대외무역 다각화를 실현하지 못해 무역대상국이 주로 중국, 한국, 일본, 러시아, 태국 등 극소수 국가에만 국한되어 있고, 특히 최근 중국, 한국에 대한 무역의존도가 약 80~90%를 차지하고 있다. 따라서 향후 중국과 한국의 대북한 경제정책의 변화는 북한의 대외 경제활동에 결정적인 영향을 미칠 것이며, 아울러 북한의 국내경제 회복과 성장·발전에도 중요한 역할을 할 것으로 예상된다. 셋째, 최근 북한은 철강, 원유, 식량, 기계, 의약품, 일용품 등 전략물자와 관련하여 심각한 대중국 의존현상을 보이고 있는데, 이러한 상황은 향후 상당기간 동안 지속될 것으로 예측된다. 넷째, 20세기 90년대 중·후반 북한경제는 붕괴의 위기를 겪었고, 외부의 원조를 통해 간신히 파국을 모면할 수 있었다. 21세기에 접어들면서 북한경제가 미약하나마 회복 성장을 시현해 가고 있지만, 최하 수준의 생계유지를 위해 여전히 국제사회의 원조에 의존하고 있으며, 그 중 중국과 한국의 지원이 절대적인 역할을 하고 있다. 특히 중국의 대 북한 식량 및 원유 원조는 북한의 생명 줄을 쥐고 있다고 해도 무방할 것이다. 다섯째, 최근 들어 북한이 2002년 ‘7.1 경제관리개선조치’에 비해 더욱 역행하는, 즉, 더욱 강화된 계획경제 조치들을 강구하고 있는 상황임을 감안할 때, 북한은 여전히 개혁·개방에 대해 폐쇄주의적 자세를 견지하고 있는 것으로 보인다. 결국, 북한이 단시일 내에 본격적인 대내개혁, 대외개방을 실시하고, 국제사회의 일원으로 등장하기는 어려울 것이며, 이로 인해 대중국 경제의존 현상은 지속 심화될 것으로 판단된다. 여섯째, 21세기로 진입하면서, 특히 한국의 이명박 정부가 출범하면서 중 북 경제관계는 역사상 그 어느 시점과 비교하여도 무역, 투자 및 기타 분야에서 양적으로나 질적으로 괄목할만한 발전을 가져왔으며 이러한 추세는 향후 수 년간 한층 더 업그레이드될 전망이다. This study analysed the dependence of the basic economy in North Korea in the first decade of the 2000s and the effects of this dependance. The results had a few implications. First, North Korea has adhered to a planned economic system of socialism only in the northeast area regardless of trends in the world economy, such as the formation of blocks and unification, and has firmly adhered to an independent national economy building policy. Therefore, as the weight and role external trade occupies in economic development was supplementary, the North`s dependence on external economic situations is extremely low. This indicates that North Korea`s economy, in particular the northeast economy, will not be greatly affected by the external world economy. However, since the second nuclear test in North Korea, American economic restrictions on North Korea have increased. Also as the Lee Government of South Korea has stopped support for North Korea, arguing against a nuclear armed North Korea, its dependence on China has increased. This is based on the recognition that North Korea can depend on China. In particular, the North`s dependence on China for strategic goods and support is acute. Second, North Korea has not realized the formation of multi-faceted external trade. It has had trade only with China, South Korea, Japan, Russia, and Thailand. In particular, the North`s trade dependence on China and South Korea amounts to between 80 and 90% of its external trade. Because of this, it is anticipated that changes in economic policy towards North Korea by China and South Korea will affect its external economic activities and, will play an important role in the recovery, growth, and development of the internal economy of North Korea. Third, North Korea has depended heavily on China for strategic goods such as steel, crude oil, food, machinery, medical supplies, and daily necessities. This dependance is expected to continue for the forseeable future. Fourth, North Korea risked a collapsing economy in the mid and late 1990s and narrowly avoided collapse through external support. In the 21st century, it has realized its recovery, but it is still dependant on support from international society in order to maintain minimum sustainability. For this, support from South Korea and China is absolutely necessary. In particular, it can be said that China`s support for North Korea in food and crude oil decides the life of North Korea. Fifth, in considering that North Korea has been pursuing more reinforced planned economic measures, which are opposite the ``7.1 Economic Control Measures`` published in 2002, it seems that North Korea is still adhering to a closed policy against innovation and opening. In conclusion, it is difficult for North Korea to execute the internal innovation or external opening necessary to appear as a member of international society, and therefore, its economic dependence will be maintained or intensified. Sixth, in the 21st century, in particular as the Lee Government launched, economic relations between North Korea and China have developed significantly in quality and quantity, and this trend will continue.

中國人民幣國際化的路徑依賴及模式按排探析

임명 ( Ming Ren ) 조선대학교 사회과학연구원 2010 서석사회과학논총 Vol.3 No.1