Lysinibacillus tabacifolii sp. nov., a Novel Endophytic Bacterium Isolated from Nicotiana tabacum Leaves

Yan-Qing Duan,Song-Tao He,Qing-Qing Li,Ming-Feng Wang,Wen-Yuan Wang,Wei Zhe,Yong-Hong Cao,Ming-He Mo,Yu-Long Zhai,Wen-Jun Li 한국미생물학회 2013 The journal of microbiology Vol.51 No.3

A Gram-positive, catalase- and oxidase-positive, strictly aerobic, endospore-forming rod bacterium, designated K3514T, was isolated from the leaves of Nicotiana tabacum. The strain was able to grow at temperatures of 8–40°C, pH 5.0–10.0 and NaCl concentrations of 0–7%. The predominant quinones (>30%) of this strain were MK-7(H2) and MK-7. Phylogenetic analysis of 16S rRNA gene sequence showed that strain K3514T was affiliated to the genus Lysinibacillus, with its closest relatives being Lysinibacillus mangiferihumi (98.3% sequence similarity), Lysinibacillus sphaericus (97.9% sequence similarity), Lysinibacillus fusiformis (97.4% sequence similarity), and Lysinibacillus xylanilyticus (97.3% sequence similarity). However, low levels of DNA-DNA relatedness values suggested that the isolate was distinct from the other closest Lysinibacillus species. Additionally, based on analysis of morphological, physiological, and biochemical characteristics, the isolate could be differentiated from the closest known relatives. Therefore, based on polyphasic taxonomic data, the novel isolate likely represents a novel species, for which the name Lysinibacillus tabacifolii sp. nov. and the type strain K3514T (=KCTC 33042T =CCTCC AB 2012050T) are proposed.

Robust Adaptive Dynamic Surface Control of Uncertain Nonlinear Systems

Ming-Zhe Hou,Guang-Ren Duan 제어·로봇·시스템학회 2011 International Journal of Control, Automation, and Vol.9 No.1

This paper studies the robust adaptive dynamic surface control of a class of nonlinear systems with unmatched uncertainties. The unmatched uncertainties consist of not only the linearly parameterized terms but also the nonlinearly parameterized terms. The bound of each nonlinearly parameterized uncertainty term is supposed to be expressed by a known nonnegative function multiplied by a constant called bound parameter. According to whether the bound parameters are known or not, two different kinds of robust adaptive dynamic surface control algorithms are proposed. It is proved that in each case all the states of the closed-loop system are kept uniformly ultimately bounded, and the output is driven to track a feasible desired output trajectory with an arbitrarily small error. An example is also employed to indicate the effect of the proposed methods.

Finite-time L2-L∞ Filtering for Nonlinear Stochastic Systems Based on a Novel Stochastic Finite-time Stability Theorem

Ming-Zhe Hou,Ai-Guo Wu,Guang-Ren Duan 제어·로봇·시스템학회 2017 International Journal of Control, Automation, and Vol.15 No.2

This paper presents a novel stochastic finite-time stability theorem and gives its application in the finitetimeL2 L¥ filter design for nonlinear stochastic systems. Different form the frequently-used stochastic finitetimestability result, the proposed one does not require that all the states have the same fractional order exponent. Based on this result, a sufficient condition is given for nonlinear stochastic systems to possess the finite-time L2 L¥ performance with a prescribed gain. Further, an existence condition of the finite-time L2 L¥ filter with aprescribed disturbance attenuation level is given for nonlinear stochastic systems with external disturbance inputs. The effectiveness of the obtained results is illustrated by an example.

Radioimmunoimaging with Mixed Monoclonal Antibodies of Nude Mice Bearing Human Lung Adenocarcinoma Xenografts

Duan, Dong,Li, Shao-Lin,Zhu, Yu-Quan,Zhang, Tao,Lei, Cheng-Ming,Cheng, Xiang-Hua Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.9

The present study was conducted to evaluate radioimmunoimaging (RII) and in vivo distribution of mixed antibodies $^{99m}Tc$-EGFR-mAb and $^{99m}Tc$-CD44-mAb in nude mice bearing human lung adenocarcinoma xenografts. Single and mixed applications of the two radiolabeled monoclonal antibodies (mAbs) were compared. Direct labeling of $^{99m}Tc$ was applied to radiolabel the EGFR and CD44 mAbs. The properties of the radiolabeled antibodies were then characterized. RII and assessment of the distribution of the antibodies in nude mice bearing lung adenocarcinoma xenografts were achieved by applying separate and combined doses of $^{99m}Tc$-EGFR-mAb and $^{99m}Tc$-CD44-mAb. The labeling rates of $^{99m}Tc$ for EGFR-mAb and CD44-mAb were $91.5%{\pm}3.8%$ and $92.3%{\pm}4.1%$ respectively, with specific activities of 2.8 and $2.9MBq/{\mu}g$, respectively, and radiochemical purities (RCP) of 96.5% and 96.2%. The radioactivity uptake of the combined application of both radiolabeled antibodies was clearly higher than with a single application of either alone. The relative values of target-to-nontarget (T/NT) measured through the regional interest (ROI) technique were $5.59{\pm}0.42$ (mixed antibodies), $2.78{\pm}0.20$ ($^{99m}Tc$-EGFR-mAb), and $2.28{\pm}0.16$ ($^{99m}Tc$-CD44-mAb) in the RII. The body distribution of the radiolabeled antibodies and their imaging results were basically identical. Application of the mixed antibodies with $^{99m}Tc$-EGFR-mAb and $^{99m}Tc$-CD44-mAb can increase the radioactivity uptake of tumor tissue, leading to more ideal target-to-nontarget ratios, and therefore superior results.

Reasearch on the main factors for changes in pressure based on turbulent circulating fluidized bed coal gasification technology

Duan Feng,Jin Bao-Sheng,Huang Ya-Ji,Li Bin,Sun Yu,Wu Yiming,Zhang Ming-Yao 한국화학공학회 2010 Korean Journal of Chemical Engineering Vol.27 No.6

High temperature preheated air and steam as gasifying agent and coal gasification was performed in a pressurized turbulent circulating fluidized bed (CFB) gasification pilot plant to investigate the pressurized gasification process and estimate its potential. Within the scope of this paper this test facility as well as its operation behavior was described. Furthermore, the parameter pressure has been investigated regarding its influence on the syngas composition and was presented and discussed in the following. The results show that the gasification quality is improved at higher pressure because of the better fluidization in the reactor. Coal gasification at a higher pressure shows advantages in lower heat value and carbon conversion. With the gasifier pressure increased from 0.1MPa to 0.3MPa, the gas heating value is increased by 15%. Increasing the gasifier pressure would increase the carbon conversion from 57.52% to 76.76%. Also, the dry gas yield and efficiency of cold gas increase little with the increase of the gasifier pressure. The operating parameter of pressure exists at optimum operating range for this specific CFB coal gasification process.

Design of Unknown Input Observer for Linear Time-delay Systems

Yan-Ming Fu,Guang-Ren Duan,Shen-Min Song 대한전기학회 2004 International Journal of Control, Automation, and Vol.2 No.4

Exploring the Key Genes and Pathways of Osteoarthritis in Knee Cartilage in a Rat Model Using Gene Expression Profiling

Yi-Ming Ren,Xin Zhao,Tao Yang,Yuan-Hui Duan,Yun-Bo Sun,Wen-Jun Zhao,Meng-Qiang Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.6

Purpose: To compare differentially expressed genes (DEGs) mediating osteoarthritis (OA) in knee cartilage and in normal kneecartilage in a rat model of OA and to identify their impact on molecular pathways associated with OA. Materials and Methods: A gene expression profile was downloaded from the Gene Expression Omnibus database. Analysis ofDEGs was carried out using GEO2R. Enrichment analyses were performed on the Gene Ontology (GO) and Kyoto Encyclopedia ofGenes and Genomes pathway using the Search Tool for the Retrieval of Interacting Genes database (http://www.string-db.org/). Subsequently, the regulatory interaction network of OA-associated genes was visualized using Cytoscape software (version 3.4.0;www.cytoscape.org). Results: In the gene expression profile GSE103416, a total of 99 DEGs were identified. Among them, 76 DEGs (76.77%) were overexpressed,and the remaining 23 DEGs (23.23%) were underexpressed. GO and pathway enrichment analyses of target geneswere performed. Using gene-gene interaction network analysis, relevant core genes, including MET, UBB, GNAI3, and GNA13,were shown to hold a potential relationship with the development of OA in cartilage. Using quantitative real-time PCR, the Gna13/cGMP-PKG signaling pathway was identified as a potential research target for therapy and for further understanding the developmentof OA. Conclusion: The results of the present study provide a comprehensive understanding of the roles of DEGs in knee cartilage in relationto the development of OA.

Almost Disturbance Decoupling for a Class of Inherently Nonlinear Systems

Yan-Ming Fu,Guang-Ren Duan,Ai-Guo Wu 제어·로봇·시스템학회 2009 International Journal of Control, Automation, and Vol.7 No.2

The problem of almost disturbance decoupling (ADD) for a class of inherently nonlinear systems is considered. The controlled systems are beyond triangular form and possess uncontrollable linearization. The performance of ADD is characterized in terms of L2-L2p gain. By using the tool of adding a power integrator, under a set of growth conditions a smooth sate feedback controller is explicitly constructed to attenuate the influence of the disturbance on the output with an arbitrary degree.

Design formulas for vibration control of sagged cables using passive MR dampers

Yuanfeng Duan,Yi-Qing Ni,Hongmei Zhang,Billie F. Jr. Spencer,Jan-Ming Ko,Shenghao Dong 국제구조공학회 2019 Smart Structures and Systems, An International Jou Vol.23 No.6

In this paper, a method for analyzing the damping performance of stay cables incorporating magnetorheological (MR) dampers in the passive control mode is developed taking into account the cable sag and inclination, the damper coefficient, stiffness and mass, and the stiffness of damper support. Both numerical and asymptotic solutions are obtained from complex modal analysis. With the asymptotic solution, analytical formulas that evaluate the equivalent damping ratio of the sagged cable-damper system in consideration of all the above parameters are derived. The main thrust of the present study is to develop an general design formula and a universal curve for the optimal design of MR dampers for adjustable passive control of sagged cables. Two sag-affecting coefficients are derived to reflect the effects of cable sag on the maximum attainable damping ratio and the optimal damper coefficient. For the cable configurations commonly used in cable-stayed bridges, the sag-affecting coefficients are directly expressed in terms of the sag-extensibility parameter to facilitate the control design. A case study on adjustable passive vibration control of the longest cable (536 m) on Stonecutters Bridge is carried out to demonstrate the influence of the sag for the damper design, and to figure out the necessity of adjustability of damper coefficients for achieving maximum damping ratio for different vibration modes.

Experimental investigation on aerodynamic behavior of a Long span cable-stayed bridge under construction

Cun-ming Ma,Qing-song Duan,Haili Liao 대한토목학회 2018 KSCE JOURNAL OF CIVIL ENGINEERING Vol.22 No.7

Su-tong Bridge is a cable-stayed bridge with a main span of 1088 m whose stiffness is small and it is sensitive to wind action. Thelength of the bridge girder at maximum single cantilever stage can reach up to 540 m, much attention should be paid in theaerodynamic performances of the under construction bridge. Based on wind tunnel tests, the aerodynamic characteristics of thegirder, free standing pylon are considered. Also, field measurements of the pressure distribution on girder surface are presented. Theexperimental results show that only torsional vibration is found in the smooth flow at wind attack angle of 3°. Considering theinfluence of turbulence, the vortex-induced vibration of the girder is disappeared. The pressure coefficient values on the uppersurface obtained from the wind tunnel tests are relatively larger than that obtained from field measurement. The drag coefficientsvalues obtained from the wind tunnel tests are about 20% larger than that from field measurement. The crane can reduce theamplitude values of the free standing pylon at an incident angle of 0°, corresponding to along bridge axis. The turbulent flow can alsoreduce the amplitude values of vortex-induced vibration.