Streptomyces xinghaiensis sp. nov., isolated from marine sediment.

Zhao, Xin-Qing,Li, Wen-Jun,Jiao, Wen-Ce,Li, Yan,Yuan, Wen-Jie,Zhang, Yu-Qin,Klenk, Hans-Peter,Suh, Joo-Won,Bai, Feng-Wu Society for General Microbiology 2009 International journal of systematic and evolutiona Vol.59 No.11

<P>A novel actinomycete, strain S187(T), was isolated from a marine sediment sample collected from Xinghai Bay, Dalian, China. Growth occurred on ISP medium 2 containing 0-9 % NaCl and at pH 6.0-9.0 and 10-45 degrees C. The cell wall of strain S187(T) contained the isomer ll-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H(6)) (40.8 %), MK-9(H(8)) (38.2 %) and MK-9(H(2)) (8.8 %). The major fatty acids were iso-C(16 : 0) (29.6 %), anteiso-C(15 : 0) (14.0 %) and anteiso-C(17 : 0) (11.6 %). Cells contained phosphatidylethanolamine, phosphatidylinositol, phosphatidylglycerol, phosphatidylinositol mannosides and one unknown phospholipid. The G+C content of the genomic DNA was 72.01 mol%. The 16S rRNA gene sequence of the isolate had similarities of 98.1 and 97.5 % with those of Streptomyces flavofuscus NRRL B-8036(T) (=DSM 41426(T)) and Streptomyces albiaxialis DSM 41799(T), respectively, showing that the novel strain should be assigned to the genus Streptomyces. DNA-DNA hybridizations with the two above-mentioned Streptomyces species showed 31.4 and 46.9 % relatedness, respectively. Moreover, the three strains differed in some physiological and biochemical properties. Thus, on the basis of phenotypic and genotypic analyses, it is proposed that strain S187(T) represents a novel species of the genus Streptomyces, for which the name Streptomyces xinghaiensis sp. nov. is proposed; the type strain is S187(T) (=NRRL B-24674(T)=CCTCC AA 208049(T)=KCTC 19546(T)).</P>

Attenuation of Beryllium Induced Hepatorenal Dysfunction and Oxidative Stress in Rodents by Combined Effect of Gallic Acid and Piperine

Zhao, Jun-Quan,Du, Guo-Zhen,Xiong, You-Cai,Wen, Yi-Fu,Bhadauria, Monika,Nirala, Satendra Kumar 대한약학회 2007 Archives of Pharmacal Research Vol.30 No.12

We determined a minimum effective dose of gallic acid (3,4,5-trihydroxy benzoic acid; 50 mg/kg, Lp.) and piperine (10 mg/kg, p.o.) through their therapeutic potential and further evaluated them individually and in combination against beryllium-induced biochemical alterations and oxidative stress-consequences in female albino rats. The administration of beryllium altered blood biochemical variables by significantly depleting hemoglobin, albumin and urea, whereas it enhanced bilirubin and creatinine. The release of serum transaminase, lactate dehydrogenase and ${\gamma}-glutamyl$ transpeptidase was significantly greater, and was concomitant with a decreasein serum alkaline phosphatase. A significant increase in lipid peroxidation and a decrease in glutathione, superoxide dismutase and catalase in the liver and kidney was an indication of oxidative stress due to beryllium exposure. Individual administration of gallic acid and piperine moderately reversed the altered biochemical variables, whereas the combination of these was found to completely reverse the beryllium-induced biochemical alterations and oxidative stress consequences. We concluded that gallic acid exerts a synergistic effect when administered with piperine and provides a more pronounced therapeutic potential in reducing beryllium-induced hepatorenal dysfunction and oxidative stress consequences.

Long Noncoding RNA FBXL19-AS1-Mediated Ulcerative Colitis-Associated Intestinal Epithelial Barrier Defect

Zhao Xun,Cui De-Jun,Yang Liu-chan,Yuan Wen-Qiang,Yan Fang 한국조직공학과 재생의학회 2022 조직공학과 재생의학 Vol.19 No.5

BACKGROUND: This study commenced to uncover the role of long non-coding RNA FBXL19 antisense RNA 1 (FBXL19-AS1) in the development of ulcerative colitis (UC) and its possible mechanism. METHODS: FBXL19-AS1 expression in the colonic sigmoid mucosa of UC patients was detected. A colitis model was induced in mice using 5% dextran sodium sulfate. Hematoxylin–eosin staining was performed for histopathological examination. Apoptosis was detected by Tunel staining and tissue fibrosis was detected by immunohistochemistry. Also, intestinal permeability was examined. The concentrations of inflammatory factors IL-1b and IL-18 were detected by enzyme-linked immunosorbent assay. The relationship between FBXL19-AS1, miR-339-3p and RHOB was verified by RNA immunoprecipitation assay and dual luciferase reporter assay. RESULTS: The expression of FBXL19-AS1 was increased in dextran sodium sulfate (DSS)-induced colitis mouse model. FBXL19-AS1 interference or miR-339-3p overexpression inhibited DSS-induced colonic epithelial cell apoptosis and inflammatory response, and improved intestinal epithelial barrier defects, thereby ameliorating DSS-induced colitis injury in mice. FBXL19-AS1 sponged miR-339-3p while miR-339-3p targeted RHOB. Overexpression of RHOB reversed the protective effect of inhibition of FBXL19-AS1 on DSS-induced colitis in mice. CONCLUSION: FBXL19-AS1 reduces miR-339-3p-mediated targeting of RHOB and aggravates intestinal epithelial barrier defect in DSS-induced colitis in mice.

Antioxidant Enzymes and Photosynthetic Responses to Drought Stress of Three Canna edulis Cultivars

Wen-E Zhang,Fei Wang,Xue-jun Pan,Zhi-guo Tian,Xiu-ming Zhao 한국원예학회 2013 원예과학기술지 Vol.31 No.6

Edible canna is a productive starch source in some tropical and semitropical regions. In these regions, water deficit stress is one of factors that limit the crop yield. In the present study, we investigated seven physiological indexes and photosynthetic responses of three edible canna (Canna edulis Ker.) cultivars (‘PLRF’, ‘Xingyu-1’, and ‘Xingyu-2’) under 35 days drought stress. Our results indicated that drought treatment caused visible wilting symptoms in all cultivars, especially in ‘Xingyu-1’. Coupled with the increase of wilting symptoms, relative water content (RWC) and chlorophyll content decreased progressively, malondialdehyde (MDA) content gradually increased, and key antioxidant enzymes such as superoxide dismutase (SOD), peroxidase (POD), catalase (CAT) activities increased first and then decreased in all three cultivars. The effect of water stress was more pronounced in ‘Xingyu-1’ than in ‘PLRF’ and ‘Xingyu-2’, and in lower leaves than in upper leaves. In addition, 35 days drought stress also significantly reduced the photosynthetic capacity. Consistent with antioxidant parameters, photosynthetic changes of ‘Xingyu-2’ were less than those of the other cultivars under water deficit stress. Drought stress caused a significant increase of water use efficiency (WUE) in ‘Xingyu-2’, but little in ‘PLRF’, and obvious decrease in ‘Xingyu-1’. These results indicated that ‘Xingyu-2’ was more tolerant to drought stress than ‘PLRF’ and ‘Xingyu-1’ by maintaining lower lipid peroxidation and higher antioxidant enzyme activities.

Percutaneous catheter drainage for abscess after surgery

Wen-Bo Zhu,Xiao-Hui Zhao,Hai-Liang Li,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Ke Zhao,Hong-Tao Hu 소화기인터벤션의학회 2022 Gastrointestinal Intervention Vol.11 No.4

Percutaneous abscess drainage (PAD) has been proven to be a safe, effective, and widely used technique for the treatment of patients with abscesses after gastrointestinal surgery. The indications for PAD are expanding and most postoperative abscesses of the gastrointestinal tract are susceptible to PAD. PAD uses various imaging methods, including ultrasound, computed tomography, and several safe and reliable catheter insertion methods.

Numerical Analysis of Solid-Liquid Two-Phase Abrasive Flow in Microcutting Polycrystalline Materials Based on Molecular Dynamics

Jun-Ye Li,Wen-Qing Meng,Kun Dong,Xin-Ming Zhang,Wei-Hong Zhao 한국정밀공학회 2018 International Journal of Precision Engineering and Vol.19 No.11

Abrasive flow polishing plays an important role as a type of precision nanomachining technology. In this study, the polishing mechanism of abrasive flow was studied using numerical simulations based on molecular dynamics. In order to study the polishing mechanism of polycrystalline materials, the molecular dynamics simulation of three kinds of polycrystalline materials was carried out. The cutting force, energy, friction coefficient, and surface morphology (during the microcutting process) of the three workpiece materials were analyzed. The analysis shows that the adhesion of atoms in the contact area on the workpiece surface will be affected by liquid phase and the type of abrasive particles, and then the surface quality polished by the abrasive flow will also be influenced. The onset of an abrasive flow polishing process involving plastic deformation (including the nucleation and migration of dislocations, grain boundary shape deformation, and deformation twinning) was observed on the workpiece surface. Defects strongly competed with each other in the form of interactions of dislocations and the grain boundaries, resulting in the migration of twin boundaries. A further analysis of the numerical results for different cutting depths and abrasive properties of the workpiece materials will be useful for understanding the different cutting effects.

Identification of a Novel Human Zinc Finger Gene, ZNF438, with Transcription Inhibition Activity

( Zhao Min Zhong ),( Bo Wan ),( Yun Qiu ),( Jun Ni ),( Wen Wen Tang ),( Xin Ya Chen ),( Yun Yang ),( Su Qin Shen ),( Ying Wang ),( Mei Rong Bai ),( Qing Yu Lang ),( Long Yu ) 생화학분자생물학회 2007 BMB Reports Vol.40 No.4

Localized surface plasmon resonance coupling in Au nanoparticles/phosphorus dendrimer multilayer thin films fabricated by layer-by-layer self-assembly method

Zhao, Wen Bo,Park, Jeongju,Caminade, Anne-Marie,Jeong, Seong-Jun,Jang, Yoon Hee,Kim, Sang Ouk,Majoral, Jean-Pierre,Cho, Jinhan,Kim, Dong Ha Royal Society of Chemistry 2009 Journal of materials chemistry Vol.19 No.14

<P>Multilayer thin films of anionic gold nanoparticles (Au<SUB>NPs</SUB>) and cationic phosphorus dendrimers were deposited on 3-(diethoxymethyl-silyl)propylamine (3-APDMES)-coated substrates using layer-by-layer (LbL) assembly driven by electrostatic interactions. The growth of Au/dendrimer multilayers composed of Au<SUB>NPs</SUB> with diameters of ∼3 nm and ∼16 nm and dendrimer with ∼2 nm diameter was monitored by UV-vis spectroscopy. The relative amounts of Au<SUB>NPs</SUB> and dendrimers in the multilayer films were calculated using a quartz crystal microbalance. The Au-containing multilayers have two surface plasmon bands at ∼530 nm and ∼600 nm, where the latter exhibits a red shift upon increasing the areal density of AuNPs as well as increasing the layer number. The localized surface plasmon resonance (LSPR) band of the hybrid films can be tuned by adding NaCl to the dendrimer solution or by removing the organic matrix. These results demonstrate that the near-field coupling between the LSPR bands of neighboring Au layers is responsible for the controlled absorption behavior. Au mesoporous films after removing dendrimers show LSPR sensing properties for alcohols with different refractive indices in the range 1.33–1.41. A linear relationship was obtained between the LSPR peak wavelength and the refractive index of the surrounding medium.</P> <P>Graphic Abstract</P><P>LSPR coupling behaviour in multilayers of gold nanoparticles (Au<SUB>NPs</SUB>) and phosphorus dendrimers is discussed, where marked blue or red shifts were observed from the LSPR band upon controlled coupling between adjacent Au<SUB>NPs</SUB>. <IMG SRC='http://pubs.rsc.org/services/images/RSCpubs.ePlatform.Service.FreeContent.ImageService.svc/ImageService/image/GA?id=b814116a'> </P>

Percutaneous catheter drainage for abscess after surgery

Wen-Bo Zhu,Xiao-Hui Zhao,Hai-Liang Li,Chen-Yang Guo,Quan-Jun Yao,Xiang Geng,Ke Zhao,Hong-Tao Hu 소화기인터벤션의학회 2022 International journal of gastrointestinal interven Vol.11 No.4

Percutaneous abscess drainage (PAD) has been proven to be a safe, effective, and widely used technique for the treatment of patients with abscesses after gastrointestinal surgery. The indications for PAD are expanding and most postoperative abscesses of the gastrointestinal tract are susceptible to PAD. PAD uses various imaging methods, including ultrasound, computed tomography, and several safe and reliable catheter insertion methods.

Enhancement of Scenedesmus sp. LX1 Biomass Production and Lipid Accumulation Using Iron in Artificial Wastewater and Domestic Secondary Effluent

( Wen Yu Zhao ),( Jun Yi Yu ),( Yin Hu Wu ),( Yu Hong ),( Hong Ying Hu ) 한국미생물생명공학회(구 한국산업미생물학회) 2014 한국미생물·생명공학회지 Vol.42 No.2

While coupling wastewater treatment with microalgal bioenergy production is very promising, new approaches are needed to enhance microalgal growth and lipid accumulation in wastewater. Therefore, this study investigated the effect of iron on the growth, nutrient removal, and lipid accumulation of Scenedesmus sp. LX1 in both artificial wastewater and domestic secondary effluents. When increasing the iron concentration from 0 to 2 mg/l in the artificial wastewater, the biomass production of Scenedesmus sp. LX1 increased from 0.17 to 0.54 g/l; the nitrogen and phosphorus removal efficiency increased from 15.7% and 80.6% to 97.0% and 99.2%, respectively; and the lipid content was enhanced 84.2%. The relationship between the carrying capacity/maximal population growth rate of Scenedesmus sp. LX1 and the initial iron concentration were also in accordance with the Monod model. Furthermore, when increasing the iron concentration to 2 mg/l in four different domestic secondary effluent samples, the lipid content and lipid production of Scenedesmus sp. LX1 was improved by 17.4-33.7% and 21.5-41.8%, respectively.