Exploring the Key Genes and Pathways of Osteoarthritis in Knee Cartilage in a Rat Model Using Gene Expression Profiling

Yi-Ming Ren,Xin Zhao,Tao Yang,Yuan-Hui Duan,Yun-Bo Sun,Wen-Jun Zhao,Meng-Qiang Tian 연세대학교의과대학 2018 Yonsei medical journal Vol.59 No.6

Purpose: To compare differentially expressed genes (DEGs) mediating osteoarthritis (OA) in knee cartilage and in normal kneecartilage in a rat model of OA and to identify their impact on molecular pathways associated with OA. Materials and Methods: A gene expression profile was downloaded from the Gene Expression Omnibus database. Analysis ofDEGs was carried out using GEO2R. Enrichment analyses were performed on the Gene Ontology (GO) and Kyoto Encyclopedia ofGenes and Genomes pathway using the Search Tool for the Retrieval of Interacting Genes database (http://www.string-db.org/). Subsequently, the regulatory interaction network of OA-associated genes was visualized using Cytoscape software (version 3.4.0;www.cytoscape.org). Results: In the gene expression profile GSE103416, a total of 99 DEGs were identified. Among them, 76 DEGs (76.77%) were overexpressed,and the remaining 23 DEGs (23.23%) were underexpressed. GO and pathway enrichment analyses of target geneswere performed. Using gene-gene interaction network analysis, relevant core genes, including MET, UBB, GNAI3, and GNA13,were shown to hold a potential relationship with the development of OA in cartilage. Using quantitative real-time PCR, the Gna13/cGMP-PKG signaling pathway was identified as a potential research target for therapy and for further understanding the developmentof OA. Conclusion: The results of the present study provide a comprehensive understanding of the roles of DEGs in knee cartilage in relationto the development of OA.

Identification of differential DNA methylated regions between different development of entomopathogenic fungi, Beauveria bassiana NCHU-157, by Nanopore sequencing

Yi-Hsuan Li,Ju-Chun Chang,Ming-Ren Yen,Yu-Feng Huang,Tzu-Han Chen,Yu-Shin Nai 한국응용곤충학회 2022 한국응용곤충학회 학술대회논문집 Vol.2022 No.10

Involvement of Nuclear Factor Kappa B in High-Fat Diet-Related Pancreatic Fibrosis in Rats

( Ming Xian Yan ),( Hong Bo Ren ),( Yi Kou ),( Min Meng ),( Yan Qing Li ) 대한소화기학회 2012 Gut and Liver Vol.6 No.3

Background/Aims: High-fat diets contribute to pancreatic fibrogenesis, but the pathogenesis remains unclear. This study investigated the role of nuclear factor kappa B (NF-κB) in high-fat diet-induced pancreatic fi brosis in rats. Methods: Male Wistar rats were fed a high-fat diet or standard normal chow for 20 weeks. Pancreatic fibrosis was determined by Sirius red staining. Immunohistochemical staining, reverse transcription-polymerase chain reaction and Western blotting were used to identify NF-κB-associated genes or protein expressions. Results: Infl ammation, fat deposition, pancreatic stellate cell activation and fi brosis were observed in the pancreases of the high-fat diet group. NF-κB subunit p65 (NF-κB/ p65) expression was localized to the nucleus, and intercellular adhesion molecule 1 (ICAM-1) was over-expressed. Pancreatic gene expression levels of NF-κB/p65, ICAM-1 and tumor necrosis factor α were all elevated signifi cantly in rats fed a high-fat diet compared with control rats. Western blotting also revealed signifi cantly increased levels of ICAM-1 and nuclear NF-κB/p65 in rats fed high-fat diets comparison with control rats. Conclusions: NF-κB is involved in high-fat diet-related pancreatic fi brosis. (Gut Liver 2012;6:381-387)

Research Articles : The Homologous Region 3 from Bombyx mori Nucleopolyhedrovirus Enhancing the Transcriptional Activity of Drosophila hsp70 Promoter

( Shun Ming Tang ),( Yong Zhu Yi ),( Ya Jing Zhou ),( Zhi Fang Zhang ),( Yi Ren Li ),( Jia Lu He ) 한국잠사학회 2004 International Journal of Industrial Entomology Vol.9 No.2

Analytical and experimental research on wind-induced vibration in high-rise buildings with tuned liquid column dampers

Liu, Ming-Yi,Chiang, Wei-Ling,Chu, Chia-Ren,Lin, Shih-Sheng Techno-Press 2003 Wind and Structures, An International Journal (WAS Vol.6 No.1

In recent years, high-strength, light-weight materials have been widely used in the construction of high-rise buildings. Such structures generally have flexible, low-damping characteristics. Consequently, wind-induced oscillation greatly affects the structural safety and the comfort of the building's occupants. In this research, wind tunnel experiments were carried out to study the wind-induced vibration of a building with a tuned liquid column damper (TLCD). Then, a model for predicting the aerodynamic response in the across-wind direction was generated. Finally, a computing procedure was developed for the analytical modeling of the structural oscillation in a building with a TLCD under the wind load. The model agrees substantially with the experimental results. Therefore, it may be used to accurately calculate the structural response. Results from this investigation show that the TLCD is more advantageous for reducing the across-wind vibration than the along-wind oscillation. When the across-wind aerodynamic effects are considered, the TLCD more effectively controls the aerodynamic response. Moreover, it is also more useful in suppressing the acceleration than the displacement in biaxial directions. As s result, TLCDs are effective devices for reducing the wind-induced vibration in buildings. Parametric studies have also been conducted to evaluate the effectiveness of the TLCD in suppressing the structural oscillation. This study may help engineers to more correctly predict the aerodynamic response of high-rise buildings as well as select the most appropriate TLCDs for reducing the structural vibration under the wind load. It may also improve the understanding of wind-structure interactions and wind resistant designs for high-rise buildings.

The Homologous Region 3 from Bombyx mori Nucleopolyhedrovirus Enhancing the Transcriptional Activity of Drosophila hsp70 Promoter

Tang, Shun-Ming,Yi, Yong-Zhu,Zhou, Ya-Jing,Zhang, Zhi-Fang,Li, Yi-Ren,He, Jia-Lu Korean Society of Sericultural Science 2004 International Journal of Industrial Entomology Vol.9 No.2

Drosophila melanogaster heat shock protein 70 gene promoter (Dhsp70p) is widely used in transgenic insect to drive exogenous gene, and the homologous region 3 from Bombyx mori nucleopolyhedrovirus (BmNPVhr3) functions as an enhancer for several promoters. To test whether BmNPVhr3 can enhance the Dhsp70ps transcriptional activity, the reporter plasmids, which contain the Dhsp70p, the reporter $\beta$-galactosidase gene with SV40 terminator and BmNPVhr3 fragment, are constructed and transfected into the insect cell lines (Bm-N cells and Sf-21 cells) by lipofectin-mediated method. The results from the transient expression assay show that BmNPVhr3 significantly increases transcriptional activity of Dhsp70p both under the normal condition and under the heat-shock treatment, although the effects are significantly different between in Bm-N cells and in sf-21 cells. The enhancing behavior of BmNPVhr3 on the Dhsp70p is in an orientation-independent manner. Meanwhile, the effects of heat-shock treatment on Dhsp70p alone or Dhsp70p/BmNPVhr3 combination present no significant difference, indicating that BmNPVhr3 only enhances the transcriptional activity of Dhsp70p, but cant alter its characteristic of the response to the heat-shock stress. The above results suggest that the Dhsp70p/BmNPVhr3 combination is more effective one to drive exogenous gene for transgene or stable cell expression system in insects.

Incidence and Mortality of Female Breast Cancer in Jiangsu, China

Wu, Li-Zhu,Han, Ren-Qiang,Zhou, Jin-Yi,Yang, Jie,Dong, Mei-Hua,Qian, Yun,Wu, Ming Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.6

Objectives: The aim of this study was to describe and analyze the incidence and mortality of female breast cancer in Jiangsu Province of China. Methods: Incidence and mortality data for female breast cancer and corresponding population statistics from eligible cancer registries in Jiangsu from 2006 to 2010 were collected and analyzed. Crude rates, age-specific rates and age-standardized rates of incidence and mortality were calculated, and annual present changes (APCs) were estimated to describe the time trends. Results: From 2006 to 2010, 11,013 new cases and 3,068 deaths of female breast cancer were identified in selected cancer registry areas of Jiangsu. The annual average crude incidence and age-standardized incidence by world population (ASW) were 25.2/ and 17.9/100,000 respectively. The annual average crude and ASW for mortality rates were 7.03/ and 4.81/100,000. The incidence was higher in urban areas than that in rural areas, and this was consistent in all age groups. No significant difference was observed in mortality between urban and rural areas. Two peaks were observed when looking at age-specific rates, one at 50-59 years and another at over 85 years. During the 5 years, incidence and mortality increased with APCs of 4.47% and 6.89%, respectively. Compared to the national level, Jiangsu is an area with relatively low risk of female breast cancer. Conclusion: Breast cancer has become a main public health problem among Chinese females. More prevention and control activities should be conducted to reduce the burden of this disease, even in relatively low risk areas like Jiangsu.

Mapping out the surgical anatomy of the lingual nerve: a systematic review and meta-analysis

Lin Sheena Xin Yi,Sim Paul Ruiqi,Lai Wei Ming Clement,Lu Jacinta Xiaotong,Chew Jacob Ren Jie,Wong Raymond Chung Wen 대한구강악안면외과학회 2023 대한구강악안면외과학회지 Vol.49 No.4

Objectives: Understanding the lingual nerve’s precise location is crucial to prevent iatrogenic injury. This systematic review seeks to determine the lingual nerve’s most probable topographical location in the posterior mandible. Materials and Methods: Two electronic databases were searched, identifying studies reporting the lingual nerve’s position in the posterior mandible. Anatomical data in the vertical and horizontal dimensions at the retromolar and molar regions were collected for meta-analyses. Results: Of the 2,700 unique records identified, 18 studies were included in this review. In the vertical plane, 8.8% (95% confidence interval [CI], 1.0%-21.7%) and 6.3% (95% CI, 1.9%-12.5%) of the lingual nerves coursed above the alveolar crest at the retromolar and third molar regions. The mean vertical distance between the nerve and the alveolar crest ranged from 12.10 to 4.32 mm at the first to third molar regions. In the horizontal plane, 19.9% (95% CI, 0.0%-62.7%) and 35.2% (95% CI, 13.0%-61.1%) of the lingual nerves were in contact with the lingual plate at the retromolar and third molar regions. Conclusion: This systematic review mapped out the anatomical location of the lingual nerve in the posterior mandible, highlighting regions that warrant additional caution during surgeries to avoid iatrogenic lingual nerve injuries.

Up-regulation of Bax and endonuclease G, and down-modulation of Bcl-XL involved in cardiotoxin III-induced apoptosis in K562 cells

Sheng-Huei Yang,Ching-Ming Chien,Mei-Chin Lu,Yi-Hsiung Lin,Xiu-Wei Hu,Shinne-Ren Lin 생화학분자생물학회 2006 Experimental and molecular medicine Vol.38 No.4

Cardiotoxin III (CTX III), a basic polypeptide with 60 amino acid residues isolated from Naja naja atra venom, has been reported to have anticancer activity. CTX III-induced K562 cell apoptosis was confirmed by DNA fragmentation (DNA ladder, sub-G1 for-mation) and phosphatidylserine (PS) externalization with an IC50 value of 1.7 g/ml at 48 h. A mechanistic analysis demonstrated that CTX III-induced apop-totic cell death was accompanied by up-regulation of both Bax and endonuclease G (Endo G), and downregulation of Bcl-XL. CTX III had no effect on the levels of Bcl-2, Bid, XIAP survivin, and AIF proteins. CTX III treatment caused loss of the mitochondrial membrane potential (Ψm), release of mitochon-drial cytochrome c to the cytosol, and activation of both caspase-9 and -3. CTX III-induced apoptosis was significantly blocked by the broad-spectrum cas-pase inhibitor Z-VAD-FMK. However, CTX III did not generate reactive oxygen species (ROS) and talase, did not block CTX III-induced apoptosis in K562 cells. Modulation of Bax, Bcl-XL, and the Endo G proteins, release of mitochondrial cytochome c, and activation of caspase-3 and -9 all are involved in the CTX III-triggered apoptotic process in human leukemia K562 cells.

Lentivirus-mediated shRNA Interference Targeting SLUG Inhibits Lung Cancer Growth and Metastasis

Wang, Yao-Peng,Wang, Ming-Zhao,Luo, Yi-Ren,Shen, Yi,Wei, Zhao-Xia Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.10

Objective: Lung cancer is a deadly cancer, whose kills more people worldwide than any other malignancy. SLUG (SNAI2, Snail2) is involved in the epithelial mesenchymal transition in physiological and in pathological contexts and is implicated in the development and progression of lung cancer. Methods: We constructed a lentivirus vector with SLUG shRNA (LV-shSLUG). LV-shSLUG and a control lentivirus were infected into the non-small cell lung cancer cell A549 and real-time PCR, Western blot and IHC were applied to assess expression of the SLUG gene. Cell proliferation and migration were detected using MTT and clony formation methods. Results: Real-time PCR, Western Blot and IHC results confirmed down-regulation of SLUG expression by its shRNA by about 80%~90% at both the mRNA and protein levels. Knockdown of SLUG significantly suppressed lung cancer cell proliferation. Furthermore, knockdown of SLUG significantly inhibited lung cancer cell invasion and metastasis. Finally, knockdown of SLUG induced the down-regulation of Bcl-2 and up-regulation of E-cadherin. Conclusion: These results indicate that SLUG is a newly identified gene associated with lung cancer growth and metastasis. SLUG may serve as a new therapeutic target for the treatment of lung cancer in the future.