황구침법(黃丘鍼法)을 이용한 고혈압 환자의 혈압강하 효과에 대한 증례보고

한창현 ( Chang Hyun Han ),황치원 ( Chi Won Hwang ),신미숙 ( Min Suk Shin ),신선화 ( Seon Hwa Shin ),최선미 ( Sun Mi Choi ) 대한경락경혈학회 2006 Korean Journal of Acupuncture Vol.23 No.3

Objectives: The aim of the study was to investigate the antihypertensive effect of Hwang-gu Acupuncture in hypertensive patients. Methods: We measured the blood pressure of the patients who were admitted in the Oriental Medical Clinic of Brother from 25th November 2005 to 27th March 2006. We included the patients only in case of the systolic blood pressure was over 140mmHg or diastolic blood pressure was over 90mmHg, Twenty two patients were treated by Hwang-gu Acupuncture. In order to evaluate the effect of the Hwang-gu Acupuncture, the blood pressure was measured before and after Acupuncture procedure total 4 times, 10times. Results: There were a significant decrease in the systolic blood pressure and significant decrease in the diastolic blood pressure by Hwang-gu Acupuncture 4 times. The effect of Hwang-gu Acupuncture by measurement time on blood pressure were follows: In a systolic blood pressure was gradually deceased significantly from 1st to 4th but diastolic blood pressure was significantly decreased after 2nd and 4th only. Conclusions: These results suggest that Hwang-gu Acupuncture is effective in decreasing the systolic and diastolic blood pressure.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

본문참조

중심어 찾기 지도 방법 연구

황현미 한국초등국어교육학회 2005 한국초등국어교육 Vol.28 No.-

중심어는 텍스트를 이해하는 데 중요한 요소이다. 그러나 학교 현장에서는 중심어 찾기에 대한 지도가 이루어지고 있지 않다. 이에 본 연구는 중심어 찾기 지도 방법에 대해 논의하고자 한다. 이를 위해 본 연구는 중심어의 개념 고찰을 시작으로 중심어 찾기 지도 방법을 세 가지 측면에서 접근해 보았다. 첫째, 중심어에 대한 정의를 내리고, 중심어를 1차 중심어, 2차 중심어로 나누었다.그리고 교육과정에서 중심어와 관련된 여러 요소를 검토하고, 교육과정 상에 나타나 있는 중심어 지도에 대한 문제점을 살펴보았다. 둘째, 중심어를 찾는 과정을 제시하고 그 안에서 중심어 찾기 방법을 체계화했다. 중심어 찾기 과정을 가시화하고, 중심어 찾기 처리 능력을 삭제와 대치 2개의 축으로 정리했다. 그리고 중심에 찾기 방법을 두 가지 반복과 상위어로 찾기로 제시하였다. 셋째, 중심어 찾기 지도 방법을 살펴보았다.중심어 찾기 방법을 정리했다고 이것이 그 대로 지도 방법이 되진 않는다. 아동들에게 가르치기 위한 체계적인 접근이 필요하다. 이를 위해 중심어 찾기 지도 방향과 지도 단계, 중심어 찾기 지도 시 고려해야 할 사항, 지도 내용의 위계화와 교육적 활용 방안을 제시하였다. 본 연구는 중심어 찾기 방법이라는 학습자 중심의 활동을 가시화 하고, 교수방법으로 체계화했다는데 그 의의를 두고 본 연구가 현장에 바로 적용되어 활용되어지기를 기대한다. The keyword is one of the most important factors to understand text, However, it was not performed to teach the method for finding keywords in language class. Therefore, this study explores the teaching method for finding keywords. For this, this paper approached the methods to find keywords with three apects. Firstly, defined what the keywords were, and divided the keywords into two groups: the first keyword, and the second keyword. In next step, searched the factors were related to the keywords in current curriculum and found problems about the factors. Secondly, reviewed a process for finding thie keywords, systematized the process, visualized the process, and summarized the ability of finding keywords as delete and replacement. In addition, suggested two finding methods that use repetition and tracking higher keywords to find correct keywords. Thirdly, suggested teaching methods for finding keywords. It is necessary to approach systematically to teach students. Even though there is an optomal method to find keywords, if there is no effective teaching method, then the optimal method isuseless. Therefor. this study suggested new concepts for teaching methods to find keywords; the teaching direction for finding keyword, the procedure for finding keywords, the factors that teachers should consider when they teach students, of teaching contents and educational application methods in real classroom. This study had a meaning that showing the systematical approach to teach students the methods to find keyword effectively. Lastly, this paper expected the suggested methods would be applied in language classrooms directly.

위선암에서 p53, MMP-2 및 TIMP-2의 표출

황호현,김의한,오미혜,조현득,양승하 순천향대학교 교수학습개발센터 2004 Journal of Soonchunhyang Medical Science Vol.10 No.3

Gastric adenocarcinoma is the most common malignant tumor in Korea. Many oncogens have been found in malignant tumor cells. Among these oncogene, MMP-2 and TIMP-2 show important role in invasivness and metastasis of malignant tumor cells. P53 is regulator of cellular proliferation, and the over expression of p53 relate to malignant transformation and prognosis. To evaluate the expression of MMP-2, TIMP-2, and p53 according to histopathologic features and the relation between these antigens and prognosis in gastric adenocarcinoma, we have performed immune staining, using mouse anti-MMP-2, mouse anti-p53, and mouse anti-TIMP-2 for 91 cases. The results were as follows; 1) The expression rates of MMP-2, TIMP-2, and p53 in gastric adenocacinoma were 58.2%, 57.1%, and 49.5%, respectively. 2) MMP-2 and TIMP-2 in gastric adenocarcinoma showed reverse correlation.(p<0.001) 3) MMP-2 showed more higher expression rates in intesinal type and sell differentiated form of gastric adenocarcinoma, and in cases showing more deeper invasion and metastasis to lymph nodes in advanced carcinoma. 4) TIMP-2 in advanced carcinoma showed more lower expression rates in cases showing more deeper invasion and metastasis to lymph nodes. 5) MMP-2 expression and TIMP-2 non-expression showed more higher frequency in intestinal type of gastric adenocarcinoma and in cases showing more deeper invasion depth in advanced carcinoma. 6) p53 in advanced carcinoma showed more higher expression rates in cases more deeper invasion and metastasis to lymph nodes. Above results indicated that MMP-2 and TIMP-2 in gastric adenocarcinoma shows reverse correlation, and may be show reverse relation according to progression, type, invasion depth, and metastasis to lymph node. The expression of p53 may be related to metastasis to lymph nodes and degree of depth in advanced carcinoma.

오매(Fructus Mume)의 항산화물질의 분리 및 특성연구

황현주,안은미,백남인,조재선,김해영 경희대학교 생명자원과학연구원 1998 硏究論文集 Vol.19 No.-

천연물로부터 새로운 항산화제를 개발하기 위한 연구의 일환으로 한방에서 사용되는 한약재 중의 하나인 오매(烏梅)의 물 추출물과 메탄을 추출물 및 순차 분획물에 대해 팜유, 우지와 돈지를 기질로하여 유지저아시험, Rancumat method, 전자공여작용 등으로 항산화성을 확인한 결과, EtOAc 분획물에서 강한 항산화 활성을 나타내어 column chromatography, thin layer chromatography를 통하여 활성성분에 관한 연구를 시도하였다. 1. 오매 용매분획물 중 EtOAc 분획물(400 ppm)은 유지저장실험(60℃)에서, 팜유, 우지와 돈지에 모두 우수한 효과를 나타내었으며, 특히 우지에서 높은 활성을 나타내었고, BHA(200 ppm)와 α-tocopherol(200 ppm)보다 활성이 높았다. 2. 오매 EtOAc 분획물(400 ppm)은 Rancimat method에서는 팜유, 우지와 돈지에 비교적 우수한 효과를 나타내었으며, 우지에서 AI가 4.39로 상당히 높은 활성을 나타내었다. 3. 오매의 용매분획물 중 EtOAc 분획물이 DPPH라디칼에 대한 강한 소거활성을 나타내었으며, 그 활성은 EtOAc 분획물의 농도에 의존하여 증가하였고, SC_(50)은 21.2μg/ml이었다. 4. 오매 EtOAc 분획물에서 분리한 8개 획분 중 E4에서 SC_(50)이 16.7μg/ml으로 높은 DPPH 라디칼 소거활성을 나타내었으며, 원인 물질은 여러 가지 스펙트럼 측정 결과 수종 물질이 혼합물인 것으로 밝혀져, 보다 순수한 형태로의 분리 후 정확한 동정의 시도가 요구되었다. Fructus Mume is the roasted hits of Prunus mwme and has been used as tradibonal Chmese medicine. The anboxidatwe effect of the extract from Fructus Mume was measured using different methodsn such as, electron donating abdihes(EDA), Rancimat and oven tests. The methanol extract obtained from the Fructus Mume was solvent-fractionated with ethyl acetate, n-butanol and water, respectively. The ethyl acetate fraction showed the highest antioxidative activity among them. To identify the compounds with antioxidative activity, the isolates from thm layer chromatography were studied further. This study demonstrates that Fructus Mume might be useful for the prevention or treatment of disorders associated with oxidative damage.

Production of Transgenic Male Piglet as Research Model for Alzheimer's Disease

Mi-Ryung Park,Kyong-Woon Kim,Jae-Seok Woo,Seongsoo Hwang,In-Sul Hwang1,Tae-Uk Kwak,Ji-Hyun Lim,Se-Pil Park 한국동물생명공학회(구 한국동물번식학회) 2017 발생공학 국제심포지엄 및 학술대회 Vol.2017 No.10

Alzheimer’s disease (AD) is a progressive neurodegenerative disease associated with memory loss and cognitive impairments. An AD transgenic (Tg) pig model would be useful for preclinical testing of therapeutic agents. In this study, we report the use of somatic cell nuclear transfer (SCNT) to produce transgenic pigs over-expressing the human AD related genes (APP, APPswedish, Presenilin 1, and Tau). Transgenic embryos were generated by SCNT of from ear fibroblasts expressing AD genes. A total of 1808 (average 258) SCNT embryos were transferred into 7 recipients. Pregnancy was successfully maintained in one recipient. We obtained 1 cloned male piglet from a surrogate gilts and the weight of piglet was 935 g. But, the male piglet died two days after birth. The piglet expressed AD related genes by PCR and western blot analysis. Transgenes were expressed in multiple tissues, and at especially high levels in brain. AD Tg pig might be very useful for studying the disease and for testing new therapeutics in preclinical studies of human AD.

콜리플라워를 이용한 피클제조 최적화

정현아,윤지영,황재선,주나미 한국식생활문화학회 2004 韓國食生活文化學會誌 Vol.19 No.2

The purpose of this study was to determine the organoleptic characterisitcs of cauliflower pickles made in various compounding ratio according to central conposite design for optimum organoleptic characteristics of the cauliflower pickles. The optimum mixing condition of cauliflower pickles were optimized, using central composite design with 3 variables and 3 levels, by response surface methodology. The various kinds of cauliflower pickle were made in various compounding ratio of vinegar, salt and sucrose - critical ingredients of pickle recipe - and were presented to reliable panels, who graded the subjects in 7 degrees for 4 items : color, lavor, hardness and overall quality. The optimum mixing conditions of cauliflower pickle were 603.50g of vinegar, 80.13g of salt and 251.079 of sucrose in the maximum point of overall quality.

농촌지역 노인들의 우울증상에 관련된 요인들의 특성

송미숙,전기홍,송현종,김정은,박승구,신상건,이동훈,정성현,최연주,최완철,최민규,황재철,홍지만 아주대학교 1997 아주의학 Vol.2 No.2

This study was performed to identify risk factors for depression among the elderly in a rural area. A survey was done for the elderly living in Gosam-myenn, Ansung-kun during the period of July 28, 1997 and July 30, 1997. Demographic and sodoeconornic characteristics, and functional and health Status were assessed using a structured questionnaire, and the level of depression was measured by GDS score. The data obtained were analyzed by frequencies, percentage, t-test, ANOVA, and multiple regression, and the results were as follows: 1. The total sample consisted of 133 elderly. Of them, 47 elderly (35.4%) showed significant depressive Symptoms. 2. The GDS score was the highest for the group over 85 years of age (p < 0.05). Those living alone or widowed showed lower GDS score than those living with someone or married (p < 0.05). 3. The GDS score was the highest in the absolutely dependent group, followed by the partially dependent group, and the independent group (p < 0.01). The elderly who perceived their health Status as excellent showed the lowest GDS score. In contrast, those who had more than five chronic diseases showed the highest GDS score. 4. The elderly receiving financial and emotional support from their family or friends showed lower GDS score compared to those without any support. 5. The economically dependent group showed higher GDS score than that of independent group. 6. The elderly who involved in leisure activities showed lower GDS score than those without any involvement. 7. In terms of risk factors for depression, we found that perceived health Status, subjective economic Status, leisure activities, living arrangement and financial support were inversely associated with GDS score, whereas labor support was positively associated with GDS score. Based upon the above findings, this study suggests that these risk factors for depression in the elderly should be taken into consideration for a comprehensive mental health program for the elderly living in the rural area.

치과용 접착제가 복합레진 인레이와 레진시멘트의 결합력에 미치는 영향

송미혜,박수정,조현구,황윤찬,오원만,황인남 大韓齒科保存學會 2008 Restorative Dentistry & Endodontics Vol.33 No.5

본 연구는 레진시멘트의 레진인레이에 대한 접착 시 접착제 혹은 primer의 사용이 결합력에 영향을 미치는지 평가하기위해 시행하였다. 직경 5 mm,높이 4.5 mm의 원기둥 형태로 제작한 레진인레이(Tescera, Bisco, USA)의 표면을 1000번,1500번 그리고 2000번 사포로 주수 하에서 연마하여 평편한 면을 형성한 후 레진인레이의 표면에 sandblasting을 시행한 후 표면에 1분 동안 silane을 도포하였다. 2군으로 나누어 한 군은 대조군으로,다른 한 군은 표면에 동일한 제조사의 접착제나 프라이머를 도포한 후 중합하였다. 레진인레이 상에 3 mm 직경의 구멍이 형성된 아크릴판을 고정하고,구멍에 레진 시멘트를 주입하여 경화시켰다. 레진 시멘트는 Panavia-F(Kyrary), Varolink-II(Ivoclar-Vivadent), RelyX Unicem (3M ESPE),Duolink (Bisco)와 자가중합형인 Multilink(Ivoclar-Vivadent)를 사용하였다. 제작된 시편을 만능물성시험기에 위치시고 전단결합강도를 측정하였다. 측정 결과 접착제나 프라이머를 도포한 군은 대조군에 비해 전단결합강도가 증가하였다 (p < 0.05). 하지만 Variolink-II와 Panavia-F는 전단결합강도의 차이를 보이지 않았다. 대조군에서는 각 레진 시멘트 간에 전단결합강도의 유의한 차이를 보였으며 (p < O.O1),Variolink-II가 가장 높은 결합강도를 보인 반면,자가중합형인 Multilink가 가장 낮은 결합강도를 보였다. 하지만,접착제나 프라이머를 도포한 군에서는 각 제품 간에 전단결합강도의 차이를 보이지 않았다. 이상의 결과 이원중합형이나 자가중합형 레진시멘을 이용하여 레진인레이 부착 시 silane 처리 후 접착제나 프라이머의 도포가 결합 강도의 증가를 위해 필요하리라 사료된다. This study analyzed the influence of dental adhesive/primer on the bond strength between indirect resin composite and the resin cement. Seventy disc specimens of indirect resin composite (Tescera Dentin. Bisco) were fabricated. And bonding area of all specimens were sandblasted and silane treated for one minute. The resin cements were used with or without application of adhesive/primer to bonding area of indirect resin restoration: Variolink-II (Ivoclar-Vivadont): Exite DSC, Panavia-F (Kuraray): ED-Primer, RelyX Unicem (3M ESPE): Single-Bond. Duolink (Bisco): One-step. Mulitlink (Ivoclar-Vivadent): Multilinh Primer. Shear bond strength was measured by Instron universal testing machine. Adhesive application improved shear bond strength (p < 0.05). But Variolink II and Panavia-F showed no statistically significant difference according to the adhesive application. With the above results. when resin inlay is luted by resin cement it seems that application of dental adhesive/primer is necessary in order to improve the bond strength.

시간 지연을 고려한 FCM 추론알고리즘

하경재,황철현,박미애 慶南大學校 附設 基礎科學硏究所 1996 硏究論文集 Vol.8 No.-

Uncertain causal knowledge is stored in Fuzzy Cognitive Maps(FCMs). FCM resonant states are limit cycles. A FCM limit cycle or hidden pattern is a FCM inference. However, there are some drawbacks concerned with inference and knowledge representation without the time delay in the conventional FCMs. In this paper, we propose an inference algorithm considered the time delay, based on improved FCMs represented causal relationships more naturally. Computer simulation results demonstrate the effectiveness of the proposed algorithm.