소염방 약침이 흰쥐 시상하부 면역 조절에 미치는 영향

이태후 ( Tae Hoo Yi ),박히준 ( Hi Joon Park ),임사비나 ( Sa Bi Na Lim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.2

목적: 본 연구는 염증유발 내독소인 LPS 자극을 통한 면역 스트레스에 대한 약침의 치료효과와 기전을 알아보기 위해 설계되었다. LPS를 통한 염증반응은 다양한 기전을 통해 이루어지는 데 특히, 산화질소 (NO)를 생성하는 효소인 iNOS의 활성은 시상하부에서 NO의 생성을 촉진함으로써 염증반응을 발현시키는 중요한 역할을 하는데 약침이 LPS로 인해 활성화된 iNOS의 발현에 어떠한 영향을 미치는지 알아보고자 하였다. 실험방법: 흰쥐에 LPS를 투여하고 2시간 경과한 후 약침 (소염방, 대한약침학회)을 경혈 (합곡, 족삼리)에 좌,우 각각 0.2cc 씩 피하 투여하고 RT-PCR 방법을 통하여 면역활성에 중요한 역할을 하는 시상하부에서의 iNOS의 mRNA 발현을 알아 보았다. 실험결과: LPS 투여로 인하여 증가하였던 iNOS mRNA가 약침치료를 통해 현저하게 감소하였다. 그러나 비경혈점 (미추부위 임의혈)에 약침을 투여한 군과 경혈점에 생리식염수를 투여한 군에서는 iNOS mRNA 가 감소하지 않았다. 이는 약침이 LPS를 투여한 흰쥐의 시상하부에서 iNOS의 mRNA 발현에 조절 작용을 가지고 있는 것을 보여주는 결과로 약침이 면역활성에 중요한 역할을 하는 NO의 생성을 억제함으로써 면역 스트레스에 치료효과를 나타내는 것으로 보여진다. 결론: 본 연구는 약침치료가 면역반응의 중추기관인 시상하부에서 면역반응의 중요한 조절인자인 NO의 생성을 조절하는 기전을 통해 그 치료효과를 나타내는 것을 밝혔고 더 나아가 약침에 대한 현대과학적 연구에 중요한 발판을 마련했다는 점에서 그 의의를 가지고 있다고 사료된다.

비만(肥滿) 치료(治療)를 위한 경혈(經穴) 및 약물(藥物)에 관한 고찰(考察)

주준성 ( Jun Sung Joo ),채우석 ( Woo Seok Chae ),신미숙 ( Mi Sook Shin ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

Objectives: This study was designed to investigate possibilities of herbal acupuncture for obesity. Methods: We researched into 23 kinds of literature and considered acupoints and herbs of herbal acupuncture for obesity. Results: Prescriptions for obesity include Erchentang(二陳湯), Liujunzitang(六君子湯) for phlegm-retention syndrome(痰飮), Wulingsan(五령散), Qianghuoshengshitang(羌活勝濕湯), Shenshitang(渗濕湯), Lingguishugantang(령桂朮甘湯) for Dampness syndrome(濕證) and Pingweisan(平胃散) for activating spleen and stomach(運化脾胃). And Rhizoma atractylodis(蒼朮), Tuber pinelliae(半夏), Rhizoma atractylodis macrocephalae(白朮), Fructus ponciri(枳殼), Fructus immaturus ponciri(枳實), Poria(茯령), Semen coicis(薏苡仁), Rhizoma alismatis(澤瀉) Fructus amomi(砂仁), Polyporus umbellatus(猪령), Medulla tetrapanacis(通草) can be used for both herbal medication and herbal acupuncture. And also there are some important acupoints for treating obesity, which are Sanyinjiao(三陰交), Pishu(脾兪), Gongsun(公孫), Fenglong(豊隆), Yinlingquan (陰陵泉), Zusanli(足三里), Qihai(氣海), Sanjiaoshu(三焦兪), Zhongwan(中脘), Neiguan(內關), Weishu(胃兪), Shenshu(腎兪) and Shangjuxu(上巨虛). Conclusions: We recommend that herbal acupuncture can be used for obesity. This method is a kind of injection using herbal extract, which can apply pharmacognosy theory and meridian system at the same time.

저용량(低容量) He-Ne 레이저침의 혈락적용(血絡適用) 연구(硏究)

김성철 ( Sung Chul Kim ),조은희 ( Eun Hee Cho ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

Objective: The purpose of this study was to investigate the significance of the Oriental medical treatment using He-Ne laser with low energy intravascular Laser Irradiation of Blood(ILIB) through the superficial venules. Methods: The investigation of details connected with the superficial venules in the literature is performed. The investigation of details connected with the pricking blood techniques through the superficial venules in the literature is performed. The classification of the pricking blood techniques through the superficial venules by the blood-letting puncture methods in the literature is performed. The arrangement of domestic clinical treatises on the effectiveness of medical treatment using He-Ne laser with low energy ILIB through the superficial venules is performed. The consideration on the methodology for the improvement of the clinical effectiveness of He-Ne laser with low energy ILIB through superficial venules is performed. Results and Conclusions: The superficial venules are small arteries, veins and capillaries in the superficial region of the human body. In the pricking blood techniques, there are the blood-letting puncture using the implement of acupuncture to the Jing points, Extra points and superficial blood vessels and the acupuncture using the Hirudo. The methods of the blood-letting puncture are classified into the venous blood-letting puncture, the pricking, the picking out white fiber-like substances from the subcutaneous tissue, the cluster needling, the scattered needling, the blood-letting puncture of the tready collateral branch of the large channel and the blood-letting puncture of skin. The He-Ne laser with low energy ILIB through the superficial venules belongs to the Oriental medical treatment as the method of the blood-letting puncture in the vein of cubital fossa. The He-Ne laser with low energy ILIB has an effect on hyperfibrinogenemia, hyperlipidemia, speech and motor dysfunction in the case of cerebral infarction, headache, dizziness, pain and numbness. It is considered that fundamental research on the biological change of the human body, the experimental animal and the unicellular animal, and research on the effectiveness and the safety, and the development of He-Ne laser with low energy ILIB of an effective wavelength range are necessary.

삼음교(三陰交), 음릉천(陰陵泉), 방광유(膀胱兪) 단독(單獨) 및 배혈(配穴) 애구(艾灸) 시술(施術)이 Uranyl Acetate에 의하여 유발(誘發)된 백서(白鼠)의 급성신부전(急性腎不全)에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),박종승 ( Jong Seung Park ),김용성 ( Young Sung Kim ),김정상 ( Jeong Sang Kim ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.2

Objectives: The aim of this experiment is to investigate the effects of moxibustion on SP6, Sp9 and BL28 in uranyl acetate-induced acute renal failure in rats. Methods: To induce experimental acute renal failure, uranyl acetate was intraperitoneally injected to rats by 5mg/kg. 3 days later moxibustion treatment was done at SP6, Sp9 and BL28 bilaterally of the rats. And then 4 days later, we measured rats` body weight, kidney weight, serum BUN, creatinine and serum electrolytic levels(Na, K and Cl). Results: In the SP6 group, serum BUN were significantly decreased (P<0.01). In the BL28 group, serum Na level were significantly decreased (P<0.05). In the SP6·SP9 group, serum creatinine level were significantly increased but serum Na level, serum Cl level were significantly decreased(P<0.05). In the SP6·BL28 group, hypertrophy index of kidney and serum creatinine level were significantly decreased(P<0.01) and serum BUN were marginally significantly decreased (0.1<P<0.05). Conclusions: Moxibustion on SP6 and BL28 by uranyl acetate-induced acute renal failure in rats can decrease hypertrophy index of kidney, serum BUN, creatinine. It appears that treatment of SP6 and BL28 moxibustion is effective on acute renal failure.

내관 전침 자극이 뇌파의 상관 차원에 미치는 영향 -정보전달 모드도해 분석법을 중심으로-

홍승원 ( Seung Won Hong ),황배연 ( Bae Yun Hwang ),이상룡 ( Sang Ryong Lee ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

The aim of this study was to examine the effects of electroacupuncture(EA) at the PC6 (Naegwan) on normal humans using KarhunenLoeve decomposition method. Electroencephalogram(EEG) is a multi-scaled signal consisting of several components of time series with different dominant frequency ranges and different origins. EEG KarhunenLoeve decomposition method exibit site-specific and state-related differences in specific frequency bands. In this study, KarhunenLoeve decomposition method was used as a measure(D2) of complexity. 30 channel EEG study was carried out in 10 subjects (10 males; age=21.4±0.5 years). Results: We found that the average values and standard deviations of D2 at FP1, FP2, FTC1, FTC2, TT1, TT2, T4, TCP1, P3, P4, T6, OZ channel (P<0.05) were higher than during the acupuncture treatment, and the average values and standard deviations of D2 at F3, F8 channels(P<0.05) were lowered than during the acupuncture treatment. However, the comparison with that before and after the treatment shows no significant differences in all channels.

족삼리(足三里)에 시술(施術)한 오수유약침(吳茱萸藥鍼)이 항암(抗癌) 및 면역기능(免疫機能)에 미치는 영향(影響)

박기홍 ( Gi Hong Park ),이병렬 ( Byung Ryul Lee ),임윤경 ( Yun Kyoung Yim ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

Objectives and methods: Study the anti-cancer, anti-metastasis and immune response improvement effects of Herbal-acupuncture with Evodiae Fructus infusion solution, we injected Evodiae Fructus infusion solution into Zusanli(St36) of C57BL/6 mouse which is corresponding to human Zusanli(St36). We observed its effect on the number of CD25+/CD4+, CD8+/CD3e+, CD69+/B220+, NK1.1+/CD3e+ cells in mouse PBMCs, the number of the pulmonary colony, MST and ILS of C57BL/6 mice implanted intravenously with B16-F10 melanoma. Results and Conclusions: The spleen cells proliferation of the sample groups treated with EDR-HAS extract has increased significantly compared with that of the control group. The percentage of the CD25+/CD4+, CD8+/CD3e+, CD69+/B220+, NK1.1+/CD3e+ cells in C57BL/6 mouse PBMCs of the sample groups treated with EDR herbal-acupuncture has increased compared with that of the control group. The lung colony number of the sample groups treated with EDR herbal-acupuncture has decreased significantly compared with that of the control group. MST and ILS of the sample groups treated with EDR herbal-acupuncture have increased significantly compared with those of the control group.

십이경근(十二經筋)과 근육(筋肉)과의 관계(關係)에 대한 연구

심원보 ( Won Bo Sim ),김용득 ( Yong Deuk Kim ),안영남 ( Young Nam An ),김경식 ( Kyung Sik Kim ),손인철 ( In Chul Sohn ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.2

The Oriental Medicine is described with so many terms of its own theory causing misunderstand of the concept which is expressed with same term used in modern medicine. Muscular system is also used in the both medicines, the Oriental Medicine and modern medicine. For the purpose of resolving the misunderstand of using the medical terms, we referred to a large number of literature for the muscular system in both medicine. Although there are few references concerning about systematic components in the Oriental Medicine, among the concepts of the Oriental Medicine, there are comparatively many approaches to the Meridian muscular system, a muscular system related with the Meridian, Therefore, there are many similarities and differences in the interrelation between the muscular system which was stated at the time of the concept of the Meridian system was developed and anatomical muscular system in the modern medicine. As a result of survey the references, we found out that anatomical muscular system is limited to visual compartments, whereas the Meridian muscular system is covering not only visual components but also the relation with internal organs. We conclude that there are conceptual differences in the kyung-keun system in the past and anatomical muscular system in the present.

족삼리(足三里), 합곡(合谷) 단일(單一) 및 배혈(配穴) 침자(鍼刺)가 상치통(上齒痛) 유발(誘發) 백서(白鼠)의 dEMG에 미치는 영향(影響)

나창수 ( Chang Su Na ),오창록 ( Chang Rock Oh ),조명래 ( Myung Rae Cho ),채우석 ( Woo Seok Chae ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.1

Objectives: The pain was induced on upper and lower incisor of the rat based on the theory of `connections of upper incisor pain with stomach meridian and lower incisor pain with large intestine meridian`. Such acupoints as LI4 and ST36 were used for alleviation of upper and lower incisor pain. Methods: The digastric myogram (dEMG) was utilized for the pain measurement. Results: The ST36 acupuncture after induction of upper incisor pain was gradually decreased or increased the dEMG. The LI4 acupuncture after induction of upper incisor pain was gradually decreased the dEMG. The ST36+LI4 acupuncture after induction of upper incisor pain was gradually decreased the dEMG. We knew this thing which the ST36+LI4 acupuncture decreased the dEMG most greatly and kept long compared to ST36 acupuncture, LI4 acupuncture. Conclusions: Acupuncture treatment at the loci of not only ST36 acupuncture but LI4 acupuncture were relieved the upper incisor pain. It was well suitable to the theory `connections of upper incisor pain with stomach meridian` that ST36 acupuncture decreased the upper incisor pain. But there was not to decrease the dEMG for the ST36 acupuncture. We are considered as tracing study continuously about ST36 acupuncture. It was not suitable to the theory `connections of upper incisor pain with stomach meridian` that LI4 acupuncture decreased the upper incisor pain. These results was considered as the function by the characteristic of the LI4 acupoint.

족삼리(足三里) 침자(鍼刺)가 인체(人體)의 Skin temperature, Galvanic skin response 및 Heart rate에 미치는 영향(影響)

윤대환 ( Dae Hwan Youn ),나창수 ( Chang Su Na ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.3

Objectives: The purpose of this study was to investigate the Effect of Acupuncture Chok-samni(ST36) on Skin temperature(ST), Galvanic skin response(GSR) and Heart rate(HR) in humans. ST36 is used in uneasiness, high fever, stroke, paralysis on oriental medicine. Methods: Subjects were 18 healthy oriental medical students volunteers(15male, 3female, mean age-28). Under sequence of ST36 acupuncture, the first group was observed ST, the second group was observed GSR and the third group was observed HR. Skin temperature was measured on Haegye acupoint(ST41) before and after acupuncture stimulation. Each test took 15minutes. This test was performed twice in order to increase the statistical authenticity. Results: Our result indicates that acupuncture stimulation of ST36 statistically decreased skin temperature on ST41. Also statistically decreased in HR. The change of GSR after ST36 acupuncture was decreased, but there is no statistical. Conclusions: These results suggested that acupuncture stimulation of ST 36 has effect on ST and HR in human body, and may be used by treatment as control the nerve system.

배합비솔(配合比率)에 따른 당귀보혈탕(當歸補血湯) 약침(藥鍼)이 빈혈백서(貧血白鼠)에 미치는 영향(影響)

진재도 ( Jae Do Jin ),김윤홍 ( Yun Hong Kim ),정태영 ( Tae Young Jung ),서정철 ( Jeong Chul Seo ),임성철 ( Seong Cheol Leem ),한상원 ( Sang Won Han ) 대한경락경혈학회 2004 Korean Journal of Acupuncture Vol.20 No.2

Objective; This study was investigated to test the anti-anemic effects of Danggwibohyeol-tang for herbal acupuncture on rat with anemia induced by phenylhydrazine according to composition ratio. Methods; Danggwibohyeol-tang herbal-acupuncture was administered into Kyoksu(膈兪穴, BL17) and RBC, Hemoglobin, Hematocrit, MCV, MCH, MCHC, RDW, platelet, MPV, PDW, indirect bilirubin and lactate dehydrogenase were tested. Results; The obtained results are summarized as follows: ① In control group RBC was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group. The effect of Danggwibohyeol-tang group C was similiar to that of Danggwibohyeol-tang group B and was higher than that of Danggwibohyeol-tang group A. ② In control group hemoglobin was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group but there was no significant difference between Danggwibohyeol-tang group A, B, C. ③ In control group hematocrit was significantly reduced in comparison with normal group. And in Danggwibohyeol-tang group A, B, C there was significant inhibitory effect on reduction in comparison with control group. The effect of Danggwibohyeol-tang group C was higher than that of Danggwibohyeol-tang group A or B. ④ In case of MCV, MCH, MCHC and RDW there was no significant anti-anemic effect. ⑤ Danggwibohyeol-tang group significantly inhibited the growth of platelet. In case of Danggwibohyeol-tang A and B group there was more inhibitory effect than Danggwibohyeol-tang C group but there was no significant difference between Danggwibohyeol-tang A and B group. ⑥ In case of MPV, PDW and indirect bilirubin there was no significant difference between Danggwibohyeol-tang group and control group. ⑦ Danggwibohyeol-tang group significantly inhibited the growth of lactate dehydrogenase. In case of Danggwibohyeol-tang C group there was more inhibitory effect than Danggwibohyeol-tang B group and Danggwibohyeol-tang B group showed more inhibitory effect than Danggwibohyeol-tang A group. Conclusion; From above results Danggwibohyeol-tang for herbal acupuncture showed significant anti-anemic effect in some parameters. Further study is needed for the effect of Danggwibohyeol-tang according to different composition ratio.