Serial values for hematologic and biochemical analysis after myocardial infarction in rats

( Mi Jin Lee ),( Hyun Jin Tae ),( Ying Hua Li ),( Do Hyeon Yu ),( In Ae Han ),( Seok Won Lee ),( Dong Choon Ahn ),( In Shik Kim ),( Jin Ho Park ) 한국가축위생학회 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction(MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hema-tologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and bioche-mical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery(LMDA) of the left coronary artery was ligated during the progression(day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group(n=24, thoracotomy without LMDA ligation) and the experimental(MI) group(n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell(RBCs), hemoglobin and hematocrit(HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, choles-terol, total creatine kinase(CK) and isoenzyme MB, and lactate dehydrogenase(LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

Serial values for hematologic and biochemical analysis after myocardial infarction in rats

Lee, Mi-Jin,Tae, Hyun-Jin,Li, Ying-Hua,Yu, Do-Hyeon,Han, In-Ae,Lee, Seok-Won,Ahn, Dong-Choon,Kim, In-Shik,Park, Jin-Ho The Korean Society of Veterinary Service 2008 韓國家畜衛生學會誌 Vol.31 No.2

To diagnose acute myocardial infarction (MI), many cardiac markers have been used in hematologic and biochemical analysis, and many studies have been published for hematologic and biochemical analysis associated with human acute MI. However, after occurrence of acute MI, the serial investigation for values in hematologic and biochemical analysis including chronic MI has rarely been performed. To observe the change of the serial values in hematologic and biochemical analysis, we induced artificial MI. The left main descending artery (LMDA) of the left coronary artery was ligated during the progression (day 1, 3, 5, 7, 14 and 30) of MI. Total 66 Sprague-Dawley rats were divided into the sham group (n=24, thoracotomy without LMDA ligation) and the experimental (MI) group (n=42, with LMDA ligation). And all individual in each group was sacrified at day 1, 3, 5, 7, 14 and 30 for the hematologic and biochemical analysis. In comparison of hematologic analysis between the sham and MI groups, the mean values of red blood cell (RBCs), hemoglobin and hematocrit (HCT) showed a steady increase. In biochemical analysis, the mean values of glucose, cholesterol, total creatine kinase (CK) and isoenzyme MB, and lactate dehydrogenase (LDH) were increased in all MI groups compared with the sham groups. The results of this study suggest that early hematologic and biochemical mean values occurred after acute MI are similar to those of human acute MI. In conclusion, we could observe the alterations and serial values in hematologic and biochemical analysis to the extent of chronic status after acute MI.

암 병동 간호사의 통증관리에 대한 지식과 태도

공성화,방찌야,서민숙,현신숙,김희정,이미애1,이미애2,유현희,허재경,김은애,박경숙 성인간호학회 2004 성인간호학회지 Vol.16 No.1

Purpose: The purpose of this study is to evaluate the knowledge and attitudes of oncology ward nurses toward cancer pain management and to find ways to improve the educational program for nurses. Method: A total of 209 nurses working at the oncology ward of three hospitals in Seoul and a Gyenggi Province. The survey instrument used was the 32-item scale for evaluating nurses knowledge and attitudes originally developed by McCaffery and Ferrell'(1990). that was by Kim'(1997). Result: In terms of the nurses knowledge of pain management. the result showed that the nurses scored an average of 67.8 out of 100 for phamacokinetics of opioids. 84.8 for classification of analgesics, 60.1 for pain assessment, and 70.7 for drug administration. 18.2% of the nurses hesitate to inject the narcotic agent because of concerns regarding the drug's potential side effects. there was significant difference in the knowledge of pain management according to the general characteristics of pain in terms of the nurses age(p=.001), position (p=.016). years of experiences(p=.002), experience of cancer pain education(p= .001). Conclusion: The also showed that nurses working at cancer ward lack knowledge. It is important to provide intensive education to nurses about cancer pain management.

항암화학요법 관련 오심과 구토 완화를 위한 비약물요법에 관한 연구동향

소향숙,최자윤,조인숙,김영재,김지영,김애숙,김옥미,김춘심,김현오,설영애,안정옥,이애리,이영자 성인간호학회 2003 성인간호학회지 Vol.15 No.3

Purpose: Purposes of this study were to understand the current trends on complementary therapy in relieving chemotherapy-induced nausea and vomiting and to suggest the future research direction. Method: Subjects were selected on CINAHL, MEDLINE, Korean Academy Data Base from 1980 to 2001 which used nausea, vomiting, chemotherapy and complementary therapy as key words in experimental studies. Eight korean articles and twenty-one international articles were analyzed in terms of general characteristics, research methods, and types of complementary therapy. Data were analyzed by using descriptive statistical methods. Result: Since 2000, researchers have more actively used complementary therapy. In subject characteristics, mean age was 35.5 years old, 45% of the researchers were performed with high level of incidence of chemotherapy induced vomiting, 14% of them set limit of consecutive cycle during research, and 65% of them did not comment the selecting criteria of sample. About 60% of them were designed post-test only control group: 35% used INV by Rhodes, 31% used Likert scale, and 24% used VAS for dependent variable. Muscle relaxation therapy was mostly applied for relief of nausea and vomiting. Conclusion: Further studies will be needed to control extrinsic variables affecting nausea and vomiting in research design and to accumulate evidence with studies applying various complementary therapies.

Phenol Stress,에 따른 Saccharomyces cerevisiae의 단백질 발현 연구

이미애,이명석 숙명여자대학교 자연과학연구소 2003 자연과학논문집 Vol.- No.14

급속히 발달하고 변화하는 주위환경의 영향으로 자연에 해로운 물질들이 많이 유입되게 되었고, 이로 인해 많은 생명들이 영향을 받게 되었다. 이에 따라 생물들은 변화하는 주위환경에 반응하여 stress proteins을 생성한다. 이번 연구에서는 yeast인 Saccaromyces cerevisiae에 환경 유해물질 중 하나인 페놀(2,4-dinitrophenol: DNP)을 처리하여 성장 단계별 protein의 발현 패턴을 two-dimensional electrophoresis system을 이용하여 연구하고 분석하였다. Web (http://www.expasy.org)에서 phenol을 처리하였을 경우에만 발현된 단백질 중 ALD4, GLK1, TRR1 유전자를 확인할 수 있었고, stationary phase에서 SSA2, SSC1, GLN1 유전자를 확인할 수 있었다. 이들의 세포내 역할에 대하여 분석, 토의하였다. Many microbial process are concerned with their constantly changing external environments to which the organism must respond quickly in order to compete successfully. As a response to harmful environmental conditions, the cell may produce additional proteins, often referred to as stress proteins. In this study was analysis of Saccharomyces cerevisiue protein expression pattern by phenol(2,4-dini-trophenol) stress. Protein separation used 2-dimensional electrophoresis system. Three proteins (Ald4p, G1k1p, Trr1p) were identified using the yeast protein map on the web(http://www.expasy.org) in exponential phase. And three proteins (Gln1p, Ssa2p, Ssc1p) were identified in statinary phase. The A1d4p has a pH 5.66 and molecular weight 53,974. The G1k1p has a pH 5.74 and molecular weight 55,245. The Trr1p has a pH 5.25 and molecular weight 34,039. The G1n1p has a pH 5.75 and molecular weight 41,616. The Ssa2p has a pH 4.66 and molecular weight 69,380. The Ssc1p has a pH 5.01 and molecular weight 68,095. A1d4p is aldehyde dehydrogenase. G1k1p is glucokinase. Trr1p is thioredoxin dependent peroxide reductase. G1n1p is glutamine synthetase. Ssa2p is heat shock protein. and, Ssc1p is chaperonin.

탈지대두박을 활용한 콩치즈와 스폰지 케익의 품질특성

이숙영,박미정,최애진 중앙대학교 생활과학연구소 2001 생활과학논집 Vol.14 No.-

The effects of the different mixing ratios(10:0, 7:3, or 5:5) of Jinpum soybean milk to defatted soybean meal milk and the different enzyme treatments with α -chymotrypsin or trypsin on the yields, physicochemical and textural properties, and sensory characteristics of soybean cheeses were studied. The effects of the different replacement amounts(15, 30, or 45%) of defatted soybean meal on the physicochemical and sensory characteristics of sponge cake were also studied. In the results of yield, pH, lightness, hardness, mouthfeel and overall quality of sensory characteristics, the mixing ratio of 7:3 and the trypsin treatment were recommended for better manufacturing of high-quality soybean cheese. In the results of moisture contents, specific gravity, and overall quality of sensory characteristics, there were no significant differences between control and the sample of 15% replacement with untreated defatted soybean meal, and also between control and that of 30% replacement with trypsin-treated. The trypsin treatment of defatted soybean meal resulted in similar texture to control, the decrease in beany flavor, softer mouthfeel, and better moistness.

기능평가를 통한 장애유아의 행동지원 실험연구 분석 : 1997~2006년 사이의 연구를 중심으로

이미애,한성희,이규옥 국립특수교육원 2007 특수교육연구 Vol.14 No.2

이 연구는 유아를 대상으로 기능평가를 통한 행동지원을 제공한 실험연구를 분석하여 현재의 전반적인 특징과 경향을 분석하고 문제행동 기능의 결과가 중재와 어떻게 연결되는지 살펴봄으로써 문제행동 중재를 위한 향후의 방향을 모색하고자 하였다. 이 연구의 분석 대상 논문은 주로 중다기초선 설계법을 사용하며 중재는 일반교실이나 가정 등의 자연스러운 환경에서 교사나 부모 등의 자연스러운 중재자에 의해서 이루어지고 있었으며 목표행동은 대상유아의 바람직한 행동보다는 부정적인 행동이 많았으며 중재자의 행동을 목표행동으로 본 연구도 5-6편이 있었다. 연구의 질적 수준에 대한 변인들 중 관찰자간 신뢰도를 제외하고는 중재충실도, 사회적 타당도, 유지, 일반화 등에 대한 파악이 부족한 것으로 나타났다. 기능평가는 주로 일반학급과 가정 등의 자연스러운 환경에서의 부모나 일반교사를 대상으로 한 인터뷰와 연구자나 가족, 교사 등에 의한 관칠 방법, 연구자에 의한 기능분석 방법이 많이 이용되었다. 기능평가 방법으로는 직접관찰과 인터뷰가 가장 많이 이용되었으며 평균 2~3가지 방법을 사용하고 있었으며 문제행동의 기능은 관심끌기가 가장 높았고 획득과 회피, 감각적 자극 순으로 나타났다. 행동의 기능에 따른 중재전략을 살펴보면 획득기능과 회피 기능을 가진 경우에는 선행사건 예방전략과 후속반응전략이 가장 많이 사용되었고 관심의 기능을 가진 경우에는 후속반응전략이, 감각적 자극을 목적으로 한 문제행동 중재에는 문제행동을 차단하는 전략이 사용되고 있었다. The purpose of this study was to analyze experimental studies that intervened young children's challenging behavior using functional assessment and to provide information and suggestions on future research. To accomplish this purpose, the elements of experimental study, the quality features of study(interobserver agreement, fidelity of intervention, social validity, maintenance and generalization of effect) and methods of functional assessment and intervention strategies were analyzed. The major findings of this study was followed. First, most of the analyzed articles used multi-baseline design, and the interventions were implemented were natural settings like classroom and home, and the interventionists were natural people like teachers and parents. Target behaviors were focused on problematic behaviors of subjects than desirable behaviors. Second, among the 5 quality features of study I chose, fidelity of intervention, social validity, maintenance and generalization of effect were identified poorly except interobserver agreement. Third, functional assessment was implemented by natural people like teachers and family members, and at the natural settings like classrooms and home routine. Interview to teachers and family members and direct observation by researchers, teachers and family members were used. The most common functional assessment methods were direct observation and interview. And 2 or 3 methods were used one study. The order of the function of problematic behavior was attention, obtain tangible, escape, and sensory stimulation. As an interventions strategies, antecedents events, alternative skills and consequent responding were applied comparatively the same rate. Based on this results, some recommendations are suggested.

거대 난소 종양의 염전 1예

김미영,이해혁,유애리,박정희,이임순,정수호,고은석,레앙소팥,배동한,김태희,이권해 순천향의학연구소 2008 Journal of Soonchunhyang Medical Science Vol.14 No.1

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중환자실 환자에서 원내 칸디다혈증 발생의 위험 인자

이유현,최희정,이미애 대한감염학회 2004 감염과 화학요법 Vol.36 No.4

Background : Nosocomial candidemia is one of the most important disease entity in hospital not only because of increasing incidence but also because of its high fatality rate. The purpose of this study is to identify risk factors predicting the development of nosocomial candidemia and to evaluate its clinical outcome of nosocomial candidemia in the intensive care unit (ICU) patients. Materials & Methods : We conducted a retrospective, matched case-control study in adult patients admitted to ICU from January 1998 through December 2002 in Ewha Womans University Mokdong hospital. Sixty cases with candidemia were retrospectively identified from the records of the Department of Clinical Microbiology. The possible risk factors for the development of nosocomial candidemia were compared between 60 patients and 120 control subjects. Results : Candida albicans was the most commonly isolated organism. In the multivariate analysis, number of concomitant bacteremia (OR=17.3), number of candida infection in other site (OR=20.6), number of antibiotics used (OR=4.2), duration of indwelling central venous catheter (OR=16.1), and insertion of arterial line for more than 8 days (OR=6.9) were identified as significant risk factors for development of candidemia. Crude mortality rate was higher in candidemia patients compared with control subjects (P=0.004). Conclusion : The best predictors of development of candidemia in ICU patients were concomitant bacteremia, candida colonization in other site, combined use of antibiotics, and long-indwelling arterial and central venous catheter. For the ICU patients with high risk factors of candidemia, we could consider the intervention to prevent emergence of candidemia. 목적 : 원내 감염 칸디다혈증은 최근 의학 기술의 발전과 함께 면역 기능이 감소된 경우 특히 발생 빈도가 증가할 뿐 아니라 높은 치사율로 그 중요성이 강조되고 있다. 이 연구의 목적은 중환자실에 입원한 환자에서 칸디다혈증 발생의 위험 요인과 이로 인한 사망률을 파악하는데에 있다. 재료 및 방법 : 1998년 1월부터 2002년 12월까지 5년간 이화여대 목동병원 중환자실에 입원하였던 환자를 대상으로 후향적으로 입원 기록을 검토하여 환자-대조군 연구를 시행하였다. 환자군은 진단 검사의학과 미생물실 자료에 의거, 칸디다혈증이 있었던 60명을 대상으로 하였고 대조군은 같은 시기에 중환자실에 입원하였으나 칸디다 혈증은 없었던 120명으로 짝지어 각기 28개의 가능성있는 위험 인자와 치사율에 대해 조사하였다. 결과 : 칸디다혈증을 유발하는 균종은 Candida albicans가 24예(40%)로 가장 흔하였다. 다변량 분석을 통해서 균혈증이 동반된 경우(OR=17.3), 칸디다가 다른 부위에서도 검출된 경우(OR=20.6), 항생제 병용이 많을수록(OR=4.2), 중심정맥관 삽입 기간이 길수록(OR=16.1), 동맥관 삽입이 8일 이상인 경우(OR=6.9)가 중환자실 환자에서 칸디다혈증 발생의 독립적인 위험인자이었다. 보통 사망률(Crude mortality)은 환자군에서 48.3%, 대조군에서 26.7%로 칸디다혈증 환자에서 사망률이 유의하게 높았다(P=0.004). 결론 : 칸디다혈증의 발생은 균혈증이 동반된 경우, 다른 부위에서 칸디다가 검출된 경우에 증가하며, 항생제 병용이 많을수록, 혈관 내에 중심정맥관이나 동맥관이 장기간 유치되어 있을수록 증가하였다. 칸디다혈증의 사망률 역시 높아, 칸디다혈증의 위험이 높은 중환자실 환자를 조기에 색출하고 칸디다혈증 발생을 예방할 수 있는 방법들에 대한 연구가 필요하겠다.

One of Ours와 A Lost Lady에 나타난 미국의 꿈

이경아,하미애 한국영어영문학회 경남지부 1997 현대영미어문학 Vol.15 No.2