Retinoic acid induces expression of Ig germ line α transcript, an IgA isotype switching indicative, through retinoic acid receptor

Mi-Hee Park,박석래,Mi-Ra Lee,Young-Ha Kim,Pyeung-Hyeun Kim 한국유전학회 2011 Genes & Genomics Vol.33 No.1

Retinoic acid (RA) is considered to possess an activity of IgA isotype switching. Thus far, TGF-β1 is known to be the most powerful IgA isotype switch factor. To elucidate the molecular mechanisms underlying the Ig germ line (GL) α transcriptional regulation by RA, we constructed three different sizes of mouse GLα promoter reporters; short-GLα(-130/+14), middle-GLα(-448/+72) and long-GLα(-3028/+72). Based on luciferase assay, RA increased the activity of all three GLα promoter reporters by approximately 2-fold and the effect was further enhanced by TGF-β1. Overexpression of Smad3/4 increased TGF-β1-induced GLα promoter activities but had no effect on RA-induced GLα promoter activities. In order to analyze the characteristics of the RA-inducible GLα promoter region,we also constructed two mutant reporters: Smad3 binding elements (SBEs)-substituted short-GLα (short-GLα mSBE)and Runx3 binding elements (RBEs)-substituted short-GLα(short-GLα mRBE) promoter reporters. Promoter activities of the two mutant reporters to RA were comparable to that of wild type reporter, while those of the two mutant reporters to TGF-β1 were markedly diminished as compared to that of WT short-GLα. Finally, RA-induced GLα transcription was virtually disappeared by LE540, an antagonist of RA receptor (RAR). Taken together, these results suggest that RA induces GLα transcription mainly through RAR pathway, where neither Smad3/4 nor Runx3 is involved.

류마티스관절염 환자의 인슐린 저항성 결정인자

이정욱 ( Joung Wook Lee ),박영은 ( Young Eun Park ),조미라 ( Mi Ra Cho ),백승훈 ( Seung Hoon Baek ),김근태 ( Geun Tae Kim ),이준희 ( Jun Hee Lee ),김성일 ( Sung Il Kim ) 대한류마티스학회 2009 대한류마티스학회지 Vol.16 No.2

Objective: Rheumatoid arthritis (RA) is associated with an increased cardiovascular events. These may be related to insulin resistance (IR). We evaluated status of IR and analyzed the relationship between IR and clinical and laboratory characteristics in patients with RA. Methods: We examined 52 RA patients (43 females) and 52 age and sex matched healthy controls. We measured Homeostasis model assessment (HOMA) IR, calculated according to fasting serum glucose and insulin. Results: In patients, age was 50.8±10.2 years old, disease duration was 42.1±30.9 months. In controls, HOMA IR was 0.62±0.30 and in patients, it was 1.28±0.50. This difference was highly significant (p<0.001). Patients with early RA (disease duration is less than 36 months) were 28, and established RA (more than 36 months) were 24. HOMA IR was significantly higher in patients with established RA (1.42±0.45) than those with early RA (1.16±0.45) (p=0.03), and significantly correlated with disease duration (r=0.36, p=0.01), BMI (r=0.36, p<0.001), total cumulative prednisolon dose (r=0.34, p=0.01). Disease duration and BMI were independent predictors for HOMA IR (p<0.01, p=0.03). Conclusion: In patients with RA, IR measured by HOMA IR was more significantly increased than that of healthy control and significantly correlated with disease duration, BMI, and total cumulative prednisolon dose; however, the determinants of HOMA IR in RA patients were disease duration and BMI.

마우스에서 조합시험법에 의한 고려홍삼의 생식발생독성 평가

김미라, 박동선, 장자영, 신선희, 이예은, 백인정, 연정민, 이세라 , 남상섭, 황방연, 노재섭, 김순선, 조대현, 남상윤, 이범준, 김윤배, 윤영원 충북대학교 동물의학연구소 2006 Journal of Biomedical and Translational Research Vol.7 No.1

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알코올의 섭취가 흰쥐의 혈액과 간의 지질대사에 미치는 영향

이명숙,김영연,김효숙,김매하,전진순,서진숙,송선미,양영재,윤원손,이미라,이효영,정승현 誠信女子大學校 生活文化硏究所 2004 生活文化硏究 Vol.18 No.-

To investigate the effects of ethanol on the lipid metabolism in blood and liver, twenty one male Sprague Dawley rats had orally taken the 25% alcohol and 50% alcohol by 5g per Kg of weight during 3 weeks after the adjusting period. For the energy balance, 85% sucrose and 45% sucrose was added in control group and 25% alcohol group each. The weight was reduced by alcohol treatment according to the levels, otherwise the weight of liver and heart was little increased in alcohol groups rather than in control without significance. Moreover, RBC, WBC, hemoglobin and hematocrit were increased by alcohol treatment according to the levels without significance. Otherwise, adjusted bilirubin levels by 100g of weight was significantly increased by alcohol treatment dose dependently. Alcohol induced RBC degradation and increased circulated bilirubin because of liver damage. For blood lipid profiles, blood cholesterol was increased in 50% of alcohol group compared to that of 25% and control groups, otherwise, blood triglycerides (TG) was increased by 30% in 25% alcohol groups rather than in control but not significantly different. The hepatic lipid composition, cholesterol levels in alcohol treatment groups was lower than those in control. The hepatic TG was not influenced by alcohol treatment. The two reasons of decreasing hepatic lipids were suggested by 85% sucrose of control for energy balance more synthesized cholesterol and TG than those in 25 or 50% alcohol groups, and by cholesterol and TG could not be synthesized because of liver damage from alcohol.

3′‐Sialyllactose as an inhibitor of p65 phosphorylation ameliorates the progression of experimental rheumatoid arthritis

Kang, Li‐,Jung,Kwon, Eun‐,Soo,Lee, Kwang Min,Cho, Chanmi,Lee, Jae‐,In,Ryu, Young Bae,Youm, Tae Hyun,Jeon, Jimin,Cho, Mi Ra,Jeong, Seon‐,Yong,Lee, Sang‐,Rae,Kim, Wook,Yang John Wiley and Sons Inc. 2018 British journal of pharmacology Vol.175 No.23

<P><B>Background and Purpose</B></P><P>3′‐Sialyllactose (3′‐SL) is a safe compound that is present in high levels in human milk. Although it has anti‐inflammatory properties and supports immune homeostasis, its effect on collagen‐induced arthritis (CIA) is unknown. In this study, we investigated the prophylactic and therapeutic effect of 3′‐SL on the progression of rheumatoid arthritis (RA) in <I>in vitro</I> and <I>in vivo</I> models.</P><P><B>Experimental Approach</B></P><P>The anti‐arthritic effect of 3′‐SL was analysed with fibroblast‐like synoviocytes <I>in vitro</I> and an <I>in vivo</I> mouse model of CIA. RT‐PCR, Western blotting and ELISA were performed to evaluate its effects <I>in vitro</I>. Histological analysis of ankle and knee joints of mice with CIA was performed using immunohistochemistry, as well as safranin‐O and haematoxylin staining.</P><P><B>Key Results</B></P><P>3′‐SL markedly alleviated the severity of CIA in the mice by reducing paw swelling, clinical scores, incidence rate, serum levels of inflammatory cytokines and autoantibody production. Moreover, 3′‐SL reduced synovitis and pannus formation and suppressed cartilage destruction by blocking secretion of chemokines, pro‐inflammatory cytokines, https://en.wikipedia.org/wiki/Matrix_metalloproteinases and osteoclastogenesis <I>via</I> NF‐κB signalling. Notably, phosphorylation of p65, which is a key protein in the NF‐κB signalling pathway, was totally blocked by 3′‐SL in the RA models.</P><P><B>Conclusions and Implications</B></P><P>3′‐SL ameliorated pathogenesis of CIA by suppressing catabolic factor expression, proliferation of inflammatory immune cells and osteoclastogenesis. These effects were mediated <I>via</I> blockade of the NF‐κB signalling pathway. Therefore, 3′‐SL exerted prophylactic and therapeutic effects and could be a novel therapeutic agent for the treatment of RA.</P>

하악골에 발생한 낭종에 관한 임상적 연구

김재곤,김미라,안수현,백병주 大韓小兒齒科學會 1997 大韓小兒齒科學會誌 Vol.24 No.3

Cystic lesions of the mandible are fairly common and usually a presumptive diagnosis is made readily, more often on the basis of roentgenographic appearance than by any other means. Occasionally, however, it is easy to fall into errors of diagnosis which may affect one's choice of treatment. The diagnosis in these situations are usually resolved by the histopathologic examination of material obtained by surgical exploration. The present discussion to the lesions which can be most easily confused in the roentgenographic interpretation, that is, the traumatic bone cyst, the dentigerous cyst, the radicular cyst. These lesions often grow to considerable size before they cause any subjective or objective symptoms. Less frequently, perhaps, the patient presents himself with the complaint of enlargement of the affected part, a discharge, or pain. On rare occasions the first sign is fracture through the cyst cavity. In any case, an adequate roentgenographic survey by means of extraoral films is essintial. This is the primary means of diagnosis. The three lesions in mandible, reported here, resembled each other roentginographically in that they were osteolytic lesions. THe follicular and radicular cysts usually have a smooth periphery and may be surrounded by a white line. The follicular cyst, slow-growing lesions, is usually associated with an unerupted tooth. The radicular cyst, the most common type of the odontogenic cyst, is always associated with a nonvital tooth, or it may persist as a "residual" radicular cyst after the causative tooth has been extracted. The traumatic bone cyst can often be differentiated from the first two in that the periphery is less definite and is irregular. It is thought that because it does not occur in older people, the lesion is self-limiting and heals spontaneously if left alone.

국내의 치매관련 간호연구논문 분석

김혜영,홍춘실,오경옥,이선영,정현숙,이지혜,남미라 충남대학교 간호과학연구소 2002 충남대 간호학술지 Vol.5 No.1

The purpose of this study was to analyze the trends of korean nursing research on dementia and to suggest the direction of future research. A total of 52 nursing research articles published from 1992 to 2001 were selected for this study. These articles were analyzed in a systematic way including time and source of publication, design of research, type of subjects, variable and tool of measurement, intervention outcome of experimental research, and theme of qualitative research. The results were as follows : 1. The nursing research about dementia has appeared since 1992 and increased rapidly since 1998. 2. Eighteen of 52 studies were composed of dissertation of graduate school, and the research designs were mostly quantitative. There were 36 nonexperimental research, 11 experimental research, and 5 qualitative research designs. 3. The subjects of studies were mostly the caregivers of dementia patients as 33 of 52 studies. 4. Most measurement instruments used for research were developed by researcher him/herself, or redesigned and translated into korean as the original foreign version was used. 5. In correlational research, the trend was to study physical conditions in relation to activities of daily living and functional status in dementia patients and psychological conditions in relation to burden, quality of life, depression, and stress in their families. 6. 2 methodological research were development of assessment tool of dementia patients in community, and assessment of burden of caregiver. 7. In experimental research, subjects were all dementia patients, and interventions were music therapy(5), light therapy(2), education program, cognitive stimulation training, validation therapy, hand massage, reminiscence, horticultural therapy, and art therapy. 8. Theme of qualitative research was all caring experience of family. There were 3 phenomenologic methodology, l ethnography and l case study. Based on these findings, the following recommendations were made: 1. It' s necessary to develop a reliable and valid instrument to measure the characteristics of dementia patients and family caregivers of dementia patients. 2. Experimental study should be repeated to prove the effect of intervention for dementia patients and their family.

불소인식정도에 대한 조사보고

김미라,백병주,김재곤,이승익,양연미 大韓小兒齒科學會 1999 大韓小兒齒科學會誌 Vol.26 No.4

저자는 1998년 6월과 7월에 전북대학교병원 소아치과에 내원한 초진환자중 설문지에 응답할 수 있는 환자 50여명과 보호자 100명을 대상으로 불소에 대해 어느정도 인식하고 있는지 알아보고자 설문지를 이용하여 조사하여 다음과 같은 결론을 얻었다. 1. 불소의 치과에서의 사용여부에 대한 인식정도는 환자 26%, 보호자 51%, 전체적으로 42.7%를 보였다. 2. 불소의 우식예방효과에 대한 인식정도는 환자 86%, 보호자 85%, 전체적으로 85.7%의 높은 비율을 보였다. 3. 이에 반해 불소도포희망 여부에 대하여 환자 46%, 보호자 44%만이 긍정적으로 답하였다. 4. 불소를 이용하거나 도포한 경험은 환자 60%, 보호자 55%로 낮은 비율을 보였다. 5. 불소의 우식예방효과에 대한 인식여부와 실제이용 정도는 일치하지 않았다. 이에 치과 종사자 및 대중매체를 통해 보다 적극적인 불소사용의 교육이 이루어진다면 불소이용은 증가할 것이다. At the department of Pediatric Dentistry in Chonbuk National University Hospital, authors conducted a survey of 50 new patients and 100 new parents regarding fluoride in order to discover what they think about fluoride. The results obtained were as follows ; 1. It answered patients 26%, parents 51% and total 42.7% in knowledge about the use of fluoride in dental clinics. 2. The knowledge about caries prevention effect of fluoride is high ; patients 86%, parents 85% and total 85.3% 3. The patients and parents who wanted to use fluoride are 46%, 44% respectively 4. The patients and parents who experienced fluoride are 60%, 55% respectively 5. It is not correspond the knowledge about caries prevention effect of fluoride and the practical method of application These results suggest that the number of patients and parents who use fluoride will increase if we advise them adequately

견/합성섬유 혼방품의 일욕염색(Ⅵ) : 산성염료/분산염료 염욕에서의 견/셀룰로오스 트리아세테이트의 염착거동 Adsorption Behavior of Acid Dyes/Disperse Dyes on Silk/Cellulose Triacetate

金公朱,박미라,강영의,김애순 全北大學校 1994 論文集 Vol.38 No.-

In one bath dyeing system of silk/cellulose triacetate(triactate) fiber blend fabric with acid/disperse dyes, adsorption behvior of acid dyes and disperse dyes on silk and triactate fabrics were examined. In the dyeing of triactate with the C.I. Disperse Red 19(Red 19) and the C.I. Disperse Red 60(Red 60) at 100℃, dye uptake with the Red 19 was higher than that with the Red 60. When the silk/triaceetate was dyed with Red 19 and the Red 60 at 100℃, dye uptake on triacetate was influenced by affinity of the dye to the silk fabric dyed together. In the treatment of the silk fabric dyed with the Red 19 and Red 60 in desorption bath with and without undyed triacetate, desorption of the dyes from the silk fabric was influenced by the affinity of the dyes to the triacetate. When the silk/triacetate was dyed with the Blue 80/Red 19 and Red 60 at 100℃, color of triacetate dyed with the Red 19 and Red 60 was not influenced by the Blue 80 but silk dyed with the Blue 80 was influenced by the Red 19.

방사선 조사 마우스에서 소장움세포의 Apoptosis 발생에 미치는 생약의 효과

김성호,안미라,나승렬,이종환,김재하,조성기,장종식,신동호 대한방사선 방어학회 2001 방사선방어학회지 Vol.26 No.1

Apoptosis의 유발을 억제하는 한약제를 파악하여 apoptosis와 관련된 정상적 또는 질병에 대한 연구에 자료를 제공하고자 방사선에 의해 유도된 apoptosis를 지표로 한의학적 처방에서 많이 사용되는 대표적 한약제(24종)의 효과를 검증하였다. 용안육, 산조인, 원지, 인삼, 복령, 목향, 천궁, 백작약, 승마, 시호 및 눈꽃동충하초 투여군에서 apoptosis는 감소되었으며, 이들 생약제는 apoptosis와 관련된 질병의 예방 및 치료에 적용할 수 있을 것이다. 추 후 이들 생약의 작용 기전에 대한 연구가 요구된다. This study was performed to determine the effect of several herbs on radiation-induced apoptosis in jejunal crypt cells. Longyanrou(Euphoris longana), Suanzaoren(Zizyphus vulgaris), Yuanzhi(Polygala tenuifolia), Rensan(Panax ginseng), Fuling(Poria cocos), Muxiang(Saussurea lappa), Chuanxiong(Cnidium officinale), Baishaoyao(Paeonia lactifolia), Shengma(Cimicifuga heracleifolia), Chaihu(Bupleurum falcatum) and Dongchongxiacao(Paecilomyces japonica) reduced the frequency of radiation-induced apoptosis(p<0.05). Although the mechanisms of this effect remain to be elucidated, these results indicated that Longyanrou, Suanzaoren, Yuanzhi, Rensan, Fuling, Muxiang, Chuanxiong, Baishaoyao, Shengma, Chaihu and Dongchongxiacao might be useful inhibitors of apoptosis, especially since these are relative nontoxic natural products.