Lycopene Protects Against Spontaneous Ovarian Cancer Formation in Laying Hens

Kazim Sahin,Engin Yenice,Mehmet Tuzcu,Cemal Orhan,Cengizhan Mizrak,Ibrahim H. Ozercan,Nurhan Sahin,Bahiddin Yilmaz,Birdal Bilir,Bulent Ozpolat,Omer Kucuk 대한암예방학회 2018 Journal of cancer prevention Vol.23 No.1

Background: Dietary intake of lycopene has been associated with a reduced risk of ovarian cancer, suggesting its chemopreventive potential against ovarian carcinogenesis. Lycopene’s molecular mechanisms of action in ovarian cancer have not been fully understood. Therefore, in the present study, we investigated the effects of lycopene on the ovarian cancer formation using the laying hen model, a biologically relevant animal model of spontaneous ovarian carcinogenesis due to high incidence rates similar to humans. Methods: In this study, a total of 150 laying hens at age of 102 weeks were randomized into groups of 50: a control group (0 mg of lycopene per kg of diet) and two treatment groups (200 mg or 400 mg of lycopene per kg of diet, or ~26 and 52 mg/d/hen, respectively). At the end of 12 months, blood, ovarian tissues and tumors were collected. Results: We observed that lycopene supplementation significantly reduced the overall ovarian tumor incidence (P < 0.01) as well as the number and the size of the tumors (P < 0.004 and P < 0.005, respectively). Lycopene also significantly decreased the rate of adenocarcinoma, including serous and mucinous subtypes (P < 0.006). Moreover, we also found that the serum level of oxidative stress marker malondialdehyde was significantly lower in lycopene-fed hens compared to control birds (P < 0.001). Molecular analysis of the ovarian tumors revealed that lycopene reduced the expression of NF-B while increasing the expression of nuclear factor erythroid 2 and its major target protein, heme oxygenase 1. In addition, lycopene supplementation decreased the expression of STAT3 by inducing the protein inhibitor of activated STAT3 expression in the ovarian tissues. Conclusions: Taken together, our findings strongly support the potential of lycopene in the chemoprevention of ovarian cancer through antioxidant and anti-inflammatory mechanisms. (J Cancer Prev 2018;23:25-36)

Zinc Picolinate in the Prevention of Leiomyoma in Japanese Quail

Nurhan Sahin,Mehmet Tuzcu,İbrahim Ozercan,Kazim Sahin,Ananda S. Prasad,Omer Kucuk 한국식품영양과학회 2009 Journal of medicinal food Vol.12 No.6

Epidemiologic studies suggest that zinc deficiency may be associated with increased risk of cancer. We investigated the effects of zinc picolinate supplementation on the development of leiomyomas, malondialdehyde (MDA), 8-isoprostane, 4-hydroxyalkenal (HAE), and 8-hydroxy-2′-deoxyguanosine (8-OHdG) levels, and heat shock protein 70 (Hsp70) expression in Japanese quails. One hundred fifty quails (6 months old) were assigned to three treatment groups consisting of 50 birds in each group. Birds were fed either a basal diet or the basal diet supplemented with 30mg or 60mg of zinc/kg of diet. The animals were sacrificed after 350 days, and the tumors were identified. Zinc picolinate supplementation did not affect the number of leiomyomas compared to control birds (P>.05). However, the tumors in zinc-fed birds were smaller than those found in control birds (P=.01) Serum MDA, 8-isoprostane, and HAE levels were lower in the treatment groups than in the control group: MDA, 1.95 versus 0.93μmol/L; 8-isoprostane, 108 versus 85pg/mL; HAE, 1.55 versus 0.96μmol/L (P=.01 for all three parameters). The concentrations of serum 8-OHdG, which is a marker of oxidative damage, in the groups were 28.5, 23.6, and 20.1ng/mL, respectively (P=.01). Hsp70 expression was significantly decreased in zinc-treated birds (P<.01). The results indicate that dietary zinc picolinate supplementation reduces the growth of spontaneously occurring leiomyomas of the oviduct in the Japanese quail. Clinical trials should be conducted to investigate the efficacy of zinc supplementation in the prevention and treatment of uterine leiomyoma in humans.

Spatial Analysis for the Prediction of Soil Organic Carbon (soc) Using Digital Soil Mapping (dsm)Techniques in Mediterranean Region

Yuksel Sahin,Hakki Emrah Erdogan,Sebahattin Keskin,Mehmet Sahin 한국토양비료학회 2014 한국토양비료학회 학술발표회 초록집 Vol.2014 No.6

Investigation of crossflow features of a slender delta wing

Mehmet O. Tasci,Ilyas Karasu,Besir Sahin,Huseyin Akilli 한국풍공학회 2020 Wind and Structures, An International Journal (WAS Vol.31 No.3

In the present work, the main features of primary vortices and the vorticity concentrations downstream of vortex bursting in crossflow plane of a delta wing with a sweep angle of Λ=70° were investigated under the variation of the sideslip angles, β. For the pre-review of flow structures, dye visualization was conducted. In connection with a qualitative observation, a quantitative flow analysis was performed by employing Particle Image Velocimetry (PIV). The sideslip angles, β were varied with four different angles, such as 0°, 4°, 12°, and 20° while angles of attack, α were altered between 25° and 35°. This study mainly focused on the instantaneous flow features sequentially located at different crossflow planes such as x/C=0.6, 0.8 and 1.0. As a summary, time-averaged and instantaneous non-uniformity of turbulent flow structures are altered considerably resulting in non-homogeneous delta wing surface loading as a function of the sideslip angle. The vortex bursting location on the windward side of the delta wing advances towards the leading-edge point of the delta wing. The trajectory of the primary vortex on the leeward side slides towards sideways along the span of the delta wing. Besides, the uniformity of the lift coefficient, CL over the delta wing plane was severely affected due to unbalanced distribution of buffet loading over the same plane caused by the variation of the sideslip angle, β. Consequently, dissimilarities of the leading-edge vortices result in deterioration of the mean value of the lift coefficient, CL.

Investigation of flexural strength and cytotoxicity of acrylic resin copolymers by using different polymerization methods

Sahin, Onur,Ozdemir, Ali Kemal,Turgut, Mehmet,Boztug, Ali,Sumer, Zeynep The Korean Academy of Prosthodonitics 2015 The Journal of Advanced Prosthodontics Vol.7 No.2

PURPOSE. The aim of this study was to appraise the some mechanical properties of polymethyl methacrylate based denture base resin polymerized by copolymerization mechanism, and to investigate the cytotoxic effect of these copolymer resins. MATERIALS AND METHODS. 2-hydroxyethyl methacrylate (HEMA) and isobutyl methacrylate (IBMA) were added to monomers of conventional heat polymerized and injection-molded poly methyl methacrylate (PMMA) resin contents of 2%, 3%, and 5% by volume and polymerization was carried out. Three-point bending test was performed to detect flexural strength and the elasticity modulus of the resins. To determine the statistical differences between the study groups, the Kruskall-Wallis test was performed. Then pairwise comparisons were performed between significant groups by Mann-Whitney U test. Agar-overlay test was performed to determine cytotoxic effect of copolymer resins. Chemical analysis was determined by FTIR spectrum. RESULTS. Synthesis of the copolymer was approved by FTIR spectroscopy. Within the conventional heat-polymerized group maximum transverse strength had been seen in the HEMA 2% concentration; however, when the concentration ratio increased, the strength decreased. In the injection-molded group, maximum transverse strength had been seen in the IBMA 2% concentration; also as the concentration ratio increased, the strength decreased. Only IBMA showed no cytotoxic effect at low concentrations when both two polymerization methods applied while HEMA showed cytotoxic effect in the injection-molded resins. CONCLUSION. Within the limitations of this study, it may be concluded that IBMA and HEMA may be used in low concentration and at high temperature to obtain non-cytotoxic and durable copolymer structure.

Assessment of nonlinear static and incremental dynamic analyses for RC structures

Mehmet Emin Oncu,Merve Sahin Yon 사단법인 한국계산역학회 2016 Computers and Concrete, An International Journal Vol.18 No.6

In this study, seismic behaviour of reinforced concrete buildings using the pushover and incremental dynamic analysis method was investigated. A numerical study was performed for a reinforced concrete frame building. Pushover analysis according to uniform and triangular load shapes and incremental dynamic analyses were performed for selected building. For the nonlinear analysis, three ground motion records were selected to ensure compatibility with the design spectrum defined in the Turkish Seismic Code. The maximum response, dynamic pushover curve, capacity curves, interstorey drifts and moment rotation curves for various element ends of the selected building were obtained. Results were compared each other and good correlation was obtained between the dynamic analyses envelope with static pushover curves for the building.

Case Report : Quadriceps Tendon Rupture due to Postepileptic Convulsion

( Mehmet Sabri Balik ),( Adem Erkut ),( Yilmaz Guvercin ),( Rifat Sahin ),( Davut Keskin ) 대한슬관절학회 2014 대한슬관절학회지 Vol.26 No.2

We present a case of quadriceps tendon (QT) rupture. QT ruptures can occur in all ages. The cause is mostly traumatic in origin. Spontaneous ruptures that are thought to result from predisposing conditions are rare. Post-convulsion QT ruptures lacking traumas in their history can be overlooked in clinical examinations. This should be born in mind by the attending physician, as early diagnosis and treatment of the condition can lead to satisfactory outcomes.

Protective Effects of the Nuclear Factor Kappa B Inhibitor Pyrrolidine Dithiocarbamate on Experimental Testicular Torsion and Detorsion Injury

Sahin Kabay,Hilmi Ozden,Gul Guven,Dilek Burukoglu,Mehmet Cengiz Ustuner,Fatma Topal,Hasan Veysi Gunes,Derya Ustuner,Cansu Ozbayer 대한약리학회 2014 The Korean Journal of Physiology & Pharmacology Vol.18 No.4

Testicular torsion results with the damage of the testis and it is a surgical emergency. Pyrrolidinedithiocarbamate (PDTC) is a low-molecular-weight antioxidant and potent inhibitor of nuclear factorkappa B (NF-κB) activation. In this study, we aimed to investigate the effects of PDTC to testiculartorsion-detorsion (T/D) injury. Forty adult male Sprague-Dawley rats were separated into four groups. A sham operation was performed in group I. In group II, torsion is performed 2 hours by 720 degreeextravaginally testis. In group III, 4 h reperfusion of the testis was performed after 2 h of testiculartorsion. In group IV, after performing the same surgical procedures as in group III, PDTC (100 mg/kg,intravenous’s) was administered before 30 min of detorsion. The testes tissue malondialdehyde (MDA),superoxide dismutase (SOD) catalase (CAT) level was evaluated. Histological evaluations were performedafter hematoxylin and eosin staining. Testicular tissue MDA levels were the highest in theT/D groups compared with treatment group. Administration of PDTC prevented a further increase inMDA levels. Significant decrease occurred in CAT and SOD levels in treatment group compared withthe control group. The rats in the treatment group had normal testicular architecture. The resultssuggest that PDTC can be a potential protective agent for preventing the biochemical and histologicalchanges related to oxidative stress in testicular injury caused by testis torsion.

COMPARISON OF REPAIR INTEGRITY AND FUNCTIONAL OUTCOMES BETWEEN KNOT-TYING AND KNOTLESS SUTURE-BRIDGE ARTHROSCOPIC ROTATOR CUFF REPAIR: A PROSPECTIVE RANDOMIZED CLINICAL TRIAL

Koray Sahin,Fatih Senturk,Mehmet Ersin,Mechmet Chodza,Ufuk Arzu,Ali Ersen 대한견주관절의학회 2021 대한견주관절학회 학술대회논문집 Vol.2021 No.3

Introduction and Background Suture-bridge (SB) rotator cuff repair (RCR) is widely used due to its superior biomechanical and clinical outcomes. However, knot-tying SB technique has been suspected to compromise the tendinous biology and to cause strangulation and medial repair failures. Therefore, knotless SB technique has been proposed to decrease retear rates. The purpose of this study is to compare clinical, structural outcomes and retear patterns between two techniques Material and Method 104 patients with full-thickness rotator cuff tear were randomly and prospectively allocated to knot-tying (group I) or knotless (group II) SB surgeries. Clinical outcome measures included range of motion values, subjective pain scores (VAS) and functional scores (Constant score). Repair integrity was evaluated with magnetic resonance imagings using Sugaya classification. Retears were also classified according to their patterns as type 1 (lateral) and type 2 (medial). 88 patients completed the follow-up period and were included to final analysis. Results Mean age of patients was 54.3 years in group I and 55.8 years in group II. Mean follow-up period was 25.4 and 23.3 months respectively. Mean pain score of group I decreased from 7.4 to 1.0 (p<0.0001). The corresponding values for group II decreased from 7.1 to 1.3 (p<0.0001). In group I, mean Constant score increased from 51.7 to 86.0 (p<0.0001) and in group II, mean Constant score increased from 49.4 to 87.2 (p<0.0001). There was not statistically significant difference between two groups regarding post-operative pain and functional scores (p>0.05). Post-operative MR imaging revealed that the retear rate was 19.0% (8/42) in group I and 28.3% (13/46) in group II (p>0.05). Type 2 failure rate was 75.0% (6/8) in group I and 23.1% (3/13) in group II (p=0.03). Conclusions Both techniques showed excellent improvement and comparable clinical outcomes. However, there was no significant difference in retear rates between techniques. Type 2 failure rate was higher in knot-tying technique.

Neutrophil-lymphocyte Ratio Findings and Larynx Carcinoma: a Preliminary Study in Turkey

Duzlu, Mehmet,Karamert, Recep,Tutar, Hakan,Karaloglu, Furkan,Sahin, Melih,Cevizci, Rasit Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.1

Background: To identify the potential prognostic role of the neutrophil/lymphocyte (N/L) ratio in larynx carcinoma. Materials and Methods: Oncologic archive charts of patients with a larynx carcinoma diagnosis between the years 2010 and 2013 were retrospectively reviewed. The inclusion criterion was to be available with hemogram test prior to diagnostic procedure. Patients undergoing septorinoplasty comprised the control group. Results: There were 65 cases in the study and 42 cases in control group meeting inclusion criteria. In general a non-significant increase in N/L ratio was observed with increasing tumor size and stage (p>0.05) in larynx carcinoma. The N/L ratio was found to be significantly higher in larynx carcinoma compared to control group (p=0.004). Conclusions: In conclusion, the N/L ratio was shown to be significantly increased in larynx carcinomas compared to control group. Further studies are needed to assess any prognostic role.