HCV-Specific Interleukin-21+CD4+ T Cells Responses Associated with Viral Control through the Modulation of HCV-Specific CD8+ T Cells Function in Chronic Hepatitis C Patients

Guo-Hua Feng,Fu-Sheng Wang,Ji-Yuan Zhang,Qing-Lei Zeng,Lei Jin,Junliang Fu,Bin Yang,Ying Sun,Tianjun Jiang,Xiangsheng Xu,Zheng Zhang,Jinhong Yuan,Liyuan Wu 한국분자세포생물학회 2013 Molecules and cells Vol.36 No.4

Interleukin-21 (IL-21)+CD4+ T cells are involved in the immune response against hepatitis B virus (HBV) by secreting IL-21. However, the role of IL-21+CD4+ T cells in the immune response against chronic hepatitis C (CHC) virus infection is poorly understood. This study aimed to investigate the role of IL-21+CD4+ T cells in CHC patients and the potential mechanisms. The study subjects in-cluded nineteen CHC patients who were grouped by viral load (low, < 106 RNA copies/ml, n = 8; high, > 106 RNA copies/ml, n = 11). The peripheral frequency of HCV-specific IL-21+CD4+ T cells was higher in the low viral load group and was negatively correlated with the serum HCV RNA viral load in all CHC patients. Meanwhile, IL-21+ cells accumulated in the liver in the low viral load group. In vitro, IL-21 treatment increased the expression of proliferation markers and cytolytic molecules on HCV-specific CD8+ T cells. In summary, these findings suggest that HCV-specific IL-21+CD4+ T cells might contribute to HCV control by rescuing HCV-specific CD8+ T cells in CHC patients.

Analysis on Modeling and Motion Simulation Based on Manipulator End Executor of Small Satellite during the Grasping Process

Zhang Yuan,Shao Junpeng,Zhang Liyuan,Dai Ye,Lai Yinan 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.10

This paper introduces the components and functions of institution aiming at docking capture link of the small satellite manipulator end executor. Putting forward to Euler method and quaternion as the theoretical basis, kinetics of grasping institution the relative position and attitude are modeling and analyzed. The axial tilt grab conditions of the simulation analysis are finished by the ADAMS software. The results of the simulation analysis for the relative position and velocity and the hand-grasp collision force are concluded.

Isolation and characterization of heat-responsive gene TaGASR1 from wheat (Triticum aestivum L.)

Liyuan Zhang,Xiaoli Geng,Haiyan Zhang,Chunlei Zhou,Aiju Zhao,Fei Wang,Yue Zhao,Xuejun Tian,Zhaorong Hu,Mingming Xin,Yingyin Yao,Zhongfu Ni;Qixin Sun,Qixin Sun,Huiru Peng 한국식물학회 2017 Journal of Plant Biology Vol.60 No.1

GA-stimulated transcript (GAST) family genes have been identified in numerous plant species. In this paper, we isolated and characterized a heat-responsive gene, TaGASR1, from heat tolerant variety TAM107. The complete ORF of TaGASR1 was cloned, which encoded a 98-kDa protein, and the sequence shared 51.52% similarity to OsGASR1. Analysis of the TaGASR1 promoter region showed that it contained a heat shock element (HSE) and several cis-elements involved in abiotic stress response and hormone signal transduction. Expression patterns of TaGASR1 revealed that it was strongly induced by stress factors, such as high temperature, drought, high salinity and oxidation, as well as the phytohormones, including MeJA, ACC and ABA, which suggested the TaGASR1 gene might participate in these stress and hormone signal transduction pathways. Transient expression of TaGASR1-GFP fusion proteins in onion epidermal cells indicated that TaGASR1 protein was localized to the cell membrane or cytosol. Further analysis showed that ectopic expression of TaGASR1 in Arabidopsis enhanced thermotolerance and reduced the accumulation of reactive oxygen species (ROS) after heat stress. Moreover, we also found that TaGASR1-overexpressing wheat improved tolerance to heat stress and oxidative stress.

스마트카 사용자체험의 긍정적 정서 및 제품가치가 생활만족도에 미치는 영향에 관한 연구

장려원 ( Zhang Liyuan ),노형진 한국품질경영학회 2017 한국품질경영학회 학술대회 Vol.2017 No.1

The Role of Wheat Germ Agglutinin in the Attachment of Pseudomonas sp. WS32 to Wheat Root

Jian Zhang,Liyuan Meng,Yuanyuan Cao,Huiping Chang,Zhongyou Ma,Leni Sun,Ming Zhang,Xinyun Tang 한국미생물학회 2014 The journal of microbiology Vol.52 No.12

Wheat germ agglutinin (WGA), which is secreted on thesurface of wheat root, has been defined as a protein that reversiblyand non-enzymatically binds to specific carbohydrates. However, little attention has been paid to the functionof WGA in the attachment of bacteria to their host plants. The aim of this study was to investigate the role of WGA inthe attachment of Pseudomonas sp. WS32 to wheat roots. Wheat roots were initially treated with double-distilled water,WGA-H (WGA solution that was heated at 100°C for 15 min)and WGA, independently. Subsequently, the roots were coincubatedwith cell solutions (109 cells/ml). A dilution platemethod using a solid nutrient medium was employed to determinethe adsorption of WS32 to wheat roots. WGA waslabeled with fluorescein isothiocyanate and detected usingthe fluorescent in situ hybridization (FISH) technique. Thenumber of adsorptive WS32 cells on wheat roots was significantlyincreased when the wheat roots were pretreatedwith WGA, compared with the control treatment (p = 0.01). However, WGA-H failed to increase the amount of bacterialcells that attached to the wheat roots because of the lossof its physiological activity. The FISH assay also revealedthat more cells adhered to WGA-treated wheat roots than tocontrol or WGA-H-treated roots. The results indicated thatWGA can mediate Pseudomonas strain WS32’s adherenceto wheat seedling roots. The findings of this study provide abetter understanding of the processes involved in plant-microbe interactions.

Microfluidic fabrication of microparticles for biomedical applications

Li, Wen,Zhang, Liyuan,Ge, Xuehui,Xu, Biyi,Zhang, Weixia,Qu, Liangliang,Choi, Chang-Hyung,Xu, Jianhong,Zhang, Afang,Lee, Hyomin,Weitz, David A. The Royal Society of Chemistry 2018 Chemical Society reviews Vol.47 No.15

<P>Droplet microfluidics offers exquisite control over the flows of multiple fluids in microscale, enabling fabrication of advanced microparticles with precisely tunable structures and compositions in a high throughput manner. The combination of these remarkable features with proper materials and fabrication methods has enabled high efficiency, direct encapsulation of actives in microparticles whose features and functionalities can be well controlled. These microparticles have great potential in a wide range of bio-related applications including drug delivery, cell-laden matrices, biosensors and even as artificial cells. In this review, we briefly summarize the materials, fabrication methods, and microparticle structures produced with droplet microfluidics. We also provide a comprehensive overview of their recent uses in biomedical applications. Finally, we discuss the existing challenges and perspectives to promote the future development of these engineered microparticles.</P>

Investigation of Rebar Exposure Issues of Diaphragm Wall and Influencing Factors Analysis

Liyuan Tong,Qiwen Guo,Hongbo Che,Mingfei Zhang,Huangsong Pan,Hongjiang Li 대한토목학회 2019 KSCE JOURNAL OF CIVIL ENGINEERING Vol.23 No.4

The exposure of rebar in diaphragm wall was discovered after excavation in more than 20 subway stations, along the lines 1 to 4 in Suzhou, China. Such phenomena were investigated from strata formation, hydrogeological condition, construction techniques, adjacent surcharge load and other aspects. Based on field investigation, in situ testing and numerical simulation, the disturbance of trenching operations and the main factors that affect the stability of the diaphragm wall during the trenching process are analyzed comprehensively. The results show that the deformation behavior and stability of slurry trench walls are mainly influenced by strata characteristics, adjacent surcharge loading, formation of bentonite filter cake on trench walls, pressure of a confined aquifer, height of slurry filling and slurry density. The underground water pressure in the confined aquifer of silty soil layers is considered as one of the main controlling factors, which is verified by the subsequently dewatering and trenching tests. During the construction process, the deformation and stability of the trench walls can be improved by adjusting and controlling the crucial controlling factors. Further, relevant remedial measures as well as the preventive methods during the process of constructing diaphragm walls and subsequent soil excavation are addressed.

Synthesis of heteroatom and metallic compound self-co-doped porous carbon derived from swine manure for supercapacitor electrodes and lead ion adsorbents

Liyuan Qin,Yang Wua,Zhiwei Hou,Shihui Zhang,Enchen Jiang 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.102 No.-

Swine manure activated carbon (SMAC) with self-co-doped metallic compounds and heteroatoms wasprepared by combining KOH activation with pyrolysis. The structure, electrochemical performance andadsorption of SMACs prepared with various activation temperatures, activation times, alkali-carbonratios and pickling steps were investigated. A hierarchical pore structure existed in all the SMACs, whichshowed excellent and significantly different properties, including a specific capacitance of 278 F/g, and ahigh SSA of 2321 m2/g (SMACHF), which retained 100% of its capacitance after 10,000 cycles at 5 A/g and aPb2+ adsorption capacity of 211.8 mg/g at room temperature (SMACNON). The SMACs contained heteroatomfunctional groups and Si, Ca and Mg compounds, which did not produce pseudocapacitors but didincrease the Pb2+ adsorption capacity and electrochemical cycling stability. The Pb2+ adsorption capacitiesof SMACNON, SMACPHF and SMACHF corresponded to the effect of the functional group content ratherthan the effect of the SSA. These porous carbons were derived from inexpensive swine manure, combinesuitable porosity with metallic elements and heteroatom doping, and have promising uses in multifunctionalapplications.

Secondary Squamous Cell Carcinoma of the Oral Cavity after Nasopharyngeal Carcinoma

Liyuan Dai,Qigen Fang,Peng Li,Junfu Wu,Xu Zhang 대한암학회 2020 Cancer Research and Treatment Vol.52 No.1

Purpose The main goal of this study was to analyze the prognosis of secondary oral squamous cell carcinoma (SCC) with a comparison with sporadic oral SCC by a matched-pair design. Materials and Methods Records of patients with surgically treated primary oral SCC were reviewed, and a total of 83 patients with previous history of radiotherapy for nasopharyngeal carcinoma (NPC) were retrospectively enrolled. A matched-pair study was performed, each NPC survivor was matched with two sporadic oral SCC patients by age, sex, primary tumor site, adverse pathologic characteristics, disease stage, neck node status, and tumor stage. The overall survival (OS) and disease-specific survival (DSS) rates were calculated by the Kaplan-Meier method; independent prognostic factors were evaluated by the Cox proportional hazards method. Results Compared with sporadic oral SCC patients, NPC survivors were less likely to be smokers (p=0.004), perineural invasion and lymphovascular invasion were more common in NPC survivors (both p < 0.001). The 5-year OS and DSS rates in NPC survivors were 47% and 54%, respectively; the 5-year OS and DSS rates in sporadic oral SCC patients were 62% and 67%, respectively; the difference was significant (both p < 0.05). In survival analysis, disease stage remained to be independent prognostic factor for both the OS and DSS. Conclusion NPC survivors had worse OS and DSS than sporadic oral SCC patients, NPC survivors were less likely to be smokers, but had higher opportunity of perineural invasion and lymphovascular invasion. Disease stage was the most important predictor for the survival in NPC survivors.

Isolation and Characterization of Plant Growth-Promoting Rhizobacteria from Wheat Roots by Wheat Germ Agglutinin Labeled with Fluorescein Isothiocyanate

Jian Zhang,Jingyang Liu,Liyuan Meng,Zhongyou Ma,Xinyun Tang,Yuanyuan Cao,Leni Sun 한국미생물학회 2012 The journal of microbiology Vol.50 No.2

Thirty-two isolates were obtained from wheat rhizosphere by wheat germ agglutinin (WGA) labeled with fluorescein isothiocyanate (FITC). Most isolates were able to produce indole acetic acid (65.6%) and siderophores (59.3%), as well as exhibited phosphate solubilization (96.8%). Fourteen isolates displayed three plant growth-promoting traits. Among these strains, two phosphate-dissolving ones, WS29 and WS31, were evaluated for their beneficial effects on the early growth of wheat (Triticum aestivum Wan33). Strain WS29and WS31 significantly promoted the development of lateral roots by 34.9% and 27.6%, as well as increased the root dry weight by 25.0% and 25.6%, respectively, compared to those of the control. Based on 16S rRNA gene sequence comparisons and phylogenetic positions, both isolates were determined to belong to the genus Bacillus. The proportion of isolates showing the properties of plant growth-promoting rhizobacteria (PGPR) was higher than in previous reports. The efficiency of the isolation of PGPR strains was also greatly increased by WGA labeled with FITC. The present study indicated that WGA could be used as an effective tool for isolating PGPR strains with high affinity to host plants from wheat roots. The proposed approach could facilitate research on biofertilizers or biocontrol agents.