축우 부루셀라병의 ELISA 진단법에 관한 연구

임윤규,이두식,박전홍,양기천,김승호,김공식,현관종,김우택,이영순 濟州大學校 農科大學 動物科學硏究所 1993 動物科學論叢 Vol.8 No.1

Enzyme-linked Immuno sorbent Assay (ELISA) for the serological diagnosis oi Bruceiia abortus was developed and compared with plate aggluhnation test Cell wall antigen was extracted from Brucella abortus 1119-3 by sonicabon and with a sodium deoxychlate solution Optimum protein concentra tion of coating antigen 0.4㎍/100㎕ protein on each microtiter plate well. Horse radish peroxidase(HRP) labled protein-G was used as a tracer of reacted antibodies ELISA confirmed the agreeable results of 40 rases out of 43 cases by plate aggulutination test ELISA diagnosed positive cases (10 out of 12) and negatiw cases (1 out of 12) with dubious sera by plate agglutination test From this results EL ISA could be used for the early diagnostic tools of Brucellosis in cattle.

기무라씨병의 치험례 -2례 증례보고-

임재호,최재원,김우경,정전은 大韓成形外科學會 1990 Archives of Plastic Surgery Vol.17 No.5

Kimura's diseas is a distinct clinico-pathologic entity characterized by proliferation of vascular endothelial cells and lymphoid hyperplasia with florid germinal centers associated with lymphocytic, histiocytic and eosinopholic infiltration. Two cases of Kimura's disease are presented. The first was 53 year-old female patient who had a slow growing mass on right parotid area. The second case was a 25 year-old female patient with two irregular growing masses on both submandibular region. Both cases had markedly increased peripheral eosinophilia with 34% in first case and 31% in second case. The first was treated by superficial parotidectomy and the second by direct surgical excision of masses. The specimens were confirmed ad Kimura's disease on histopathologic examination and the two cases are presented with the review of literature.

Taxol합성에 관한 연구. Ⅱ : 3-(2-Hydroxyethyl)-2, 2, 4-trimethyl-3-cyclohexenone SEM ether의 합성

임남수,신동수,정윤성,김정주,박두천,주우홍 昌原大學校 基礎科學硏究所 1995 基礎科學硏究所論文集 Vol.7 No.-

3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-cyclohexenone SEM ether ??, an intermediate in the synthesis of taxol was provided in eight steps. The keto ketal ?? was obtained in three steps from 1,3-cyclohexanedione. Reaction of the potassium enolate of ?? with N-phenyltrifluoromethanesulfonimide provided triflate ?? in 84% yield. Thus palladium-mediated cross-coupling reaction of enol triflate ?? with vinyltributylstannane afforded diene ?? in a 89% yield. 3-(2-Hydroxyethyl)-2,2,4-trimethyl-3-Cyclohexenone SEM ether was prepared from diene ?? by sequential hydroboration [9-BBN, THF, 89%], deprotection[pTsOH ·H₂O, THF-H₂O,~100%], and silyation [SEMC], iPr₂NEt, nBu₄NI, CH₂Cl₂, 94%]

Nd:YAG Contact Laser를 이용한 새로운 미세혈관문합술의 실험적 연구

김우경,정전은,박승하,임재호,조진환 大韓成形外科學會 1993 Archives of Plastic Surgery Vol.20 No.3

A comparative study was undertaken to evaluate the contact Neodymium : yttrium aluminum garnet(Nd : YAG) laser system for vascular anastomosis of small caliber blood vessels(diameter 0.5-1.2mm) in the animal model. In this study 40 femoral arteries and 40 femoral veins of Sprague-Dawley rats were anastomosed by contact laser assisted microvascular anastomosis(LAMA) utilizing 3 stay sutures which were placed 120 degrees apart and the intervals welded with contact Nd : YAG laser unit, conventionally sutured anastomosis(CSA) served as controls. The time needed for vascular anastomosis, patency rate (immediate postoperative, postoperative 2nd day, postoperative 1 week, postoperative 4 week), gross and microscopic evaluations were compared to conventional microsurgical suture technique. The results are as follows : (1)Post-operative patency rate was 82.5% for femoral artery and 75% for femoral vein by contact LAMA technique compared to 90% and 75% by CSA technique at post operative 4 weeks. (2)Less time-consumed for arterial anastomosis by 6 minutes 23 seconds and venous anastomosis by 8 minutes 55 seconds with contact LAMA technique compared to CSA technique. (3)Grossly almost complete healing had taken place by post-operative 1 week by contact LAMA technique. (4)Aneurysm formation was 5% for femoral artery and 15% for femoral vein by contact LAMA technique compared to 5% and 10% respectively by CSA technique. (5)Microscopically, re-endothelization was complete by post-operative 7th day by contact LAMA technique. There was less medial hypertrophy and hyperplasia and also less inflammatory response compared to CSA.

근자외선 레이저를 위한 Ar 섬광관의 방전 특성과 BBQ의 형광 강도

吳喆漢,林春雨,朴成珍 慶北大學校 1992 論文集 Vol.52 No.-

The fluorescence characteristics of near UV dye, BBQ, pumped by Ar flashlamp have been studied for various electrical input energies applied to the flashlamp and argon fill pressures in the flash tube. The peak wevelength of fluorescence is about 380nm when the fluorescence intensity is at the peak value. The rise time of the fluorescence pluse is about 7μsec and its FWHM is about 55μ sec When the input energy is 72J. The fluorescence energy increases linearly with the input energy and the fluorescence energy in creases as Ar fill pressure in the flash tube increases, but it decreases over 170Torr of Ar pressure. Therefore, the optimum pressure of the tube for fluorescence emission is 170Torr.

조명광원의 색온도와 조도 및 스펙트럼 측정

최대욱,박성진,임춘우,석성수,오철한 慶北大學校 師範大學 科學敎育硏究所 1999 科學敎育硏究誌 Vol.23 No.-

The peak wavelength and temperatures of W-Halogen standard light source were measured by using the modified Wien's Law. In the case of graybody, the emissivity ε should be taken into account in using the Wien's Law. In the experiment, PDA spectroscope was used for the measurement of peak wavelength of intensity distribution of spectrum. As the current of light source increases from 5A to 7.4A, the peak wavelength of intensity distribution of the spectrum decreased from 717nm to 690nm, and temperature increased from 3127K to 3330K. Illuminations of three-wavelength lamps and light source were also measured. It was found that irradiation spectrum of those three-wavelength lamps are not coincident to blue(450nm), green(550nm) and red(610nm).

피틴산(Phytic acid)이 비브리오(Vibrio vulnificus) 패혈증에 미치는 영향

정영호,조천휘,이선우,임치환 충남대학교 농업과학연구소 2005 농업과학연구 Vol.32 No.1

Phytic acid chelates excellently the metallic ions and the positive ions, especially has high affinity with Fe^(2+) and Ca^(2+). Merits of phytic acid can be taked in easily, edibile and harmless to body, so it was investigated that phytic acid can be substituted for EDTA in this study. 1. The Intensificative effect of chelating agent and disinfective osmotic shock of Vibrio vulnificus The number of initial existent fungi measured 1.7x10^(6). The percentages of the survival fungi against the osmotic shock by distillated water were calculated at 1 minute, 3 minute and 5 minute after inoculation. The percentages of the survival fungi in Mg^(2+) were 92.5%, 91.8% and 79.8% at each time, the average percentage was 88%. Also the sudden extinction was observed around 1 minute after inoculation and the survival fungi were not observed from 3 through 5 minute in spite of repeated experimentation. 2. Influence of Vibrio vulnificus on the survival of the mice. The first mouse started to die in 180 minute after inoculation in case that the inoculating number was 2.3x10^(7) cfu/ml. All died within 4.5 hour. The average of survival time was 226 minute. The first mouse started to die in 228 minute after inoculation in case that the inoculating number was 0.8x10^(6) cfu/ml. All died within 5 hour. The average of survival time was 300 minute and the survival time was 1.3 times high. The tendencies of death in two cases were similar, but the fatal rate were largely dependent on inoculating number.

Clinical and histological significance of atypical glandular cells on cervical cytology(초)

최홍준 ( Hong Jun Choi ),( Woo Chul Kim ),( Ho Soap Hahn ),( Seok Geun Yoon ),( Yong Soon Kwon ),( In Ho Lee ),( Kyung Taek Lim ),( Ki Heon Lee ),( Jae UK Shim ),( Jung Eun Mok ),( Yi Kyeong Chun2 ),( Ta 대한산부인과학회 2009 대한산부인과학회 학술대회 Vol.95 No.-

한국인의 급성 골수성 백혈병 및 골수 이형성 증후군과 HLA 연관성

윤정숙,김원일,한경자,김용구,임지향,민우성,김춘추 대한조혈모세포이식학회 2000 대한조혈모세포이식학회지 Vol.5 No.1

배경:주조직 적합항원 복합체(Major Histocompatibility Complex : MHC)는 쥐에서 처음 이식 항원으로 발견된 이래 이식 면역 분야에서 광범위하게 연구되어 왔으며, 면역 반응을 조절하는 주요 유전자로서 감염, 종양, 자가면역 질환 등의 발생에 관여한다. 사람에서 자가 면역 질환과 HLA 연관성이 증명되어 있으나, 백혈병에서 HLA 연관성에 관한 연구는 드물며 대상군 수가 유의한 결론을 얻기에 부족하다. 본 연구에서는 급성 골수성 백혈병(AML)과 골수 이형성 증후군(MDS) 환자를 대상으로 HLA class I, II 항원 및 2-유전자좌 일배체형 빈도를 구하고, 이를 정상 대조군과 비교하는 통계적 방법으로 AML 및 MDS군과 연관된 HLA 항원 및 일배체형이 있는지 살펴보고, AML군은 FAB 분류와 면역 표현형 분류에 따른 아군으로 분류하여 각 아군과 연관된 HLA 항원 및 일배체형을 관찰함으로써 AML 및 MDS군과 HLA의 연관성을 규명해 보고자 하였다. 방법:1991년 1월부터 1998년 7월까지 성모병원에 내원하여 NIH standard microlymphocytotoxicity를 이용한 혈청학적 방법으로 HLA class I, II 항원 형별 검사를 실시한 415례의 AML과 62례의 MDS 환자를 대상으로 하여 각 질환군과 FAB, 면역 표현형 아군에서 HLA 항원 빈도와 유전자 빈도를 구하고, 항원 빈도를 대조군과 비교하여 Haldane’s method에 따라 상대 위험도(RR)를 구한 뒤 chi-square 법으로 유의성을 검증하였다. AML, MDS 각 질환군과 아군에서 square root법을 이용하여 HLA A-B, C-B, B-DR 2-유전자좌 일배체형 빈도를 구하고, 이를 대조군과 비교하여 chi-square 법으로 유의성을 검증하였다. 결과:1) Cw8이 AML, MDS 질환군에서 RR이 0.10, 0.22로 매우 감소하여 이들 질환과 강한 연관성 정도를 나타냈다(p<0.001). 2) Blank 유전자 빈도의 증가가 AML, MDS 질환군의 각 HLA locus에서 관찰되었으며, 특히 이 분석 결과는 DR locus에서 현저하여, AML과 MDS군에서 DR-blank 빈도는 11.6%, 7.0%로 매우 증가하였다. 3)AML군과 A2-B61, Cw8-B61, B62-DR11 일배체형 연관성과, MDS군과 Cw3-B51, B62-DR14, B35-DR9 일배체형 연관성이 관찰되었다. 4)FAB 분류별 아군에서 AML-M3와 A33 항원 연관성과 AML-M1과 A2-B51 일배체형 연관성이 높은 통계적 유의성을 나타내었다(p<0.001). 5) AML 면역 표현형 아군에서 HLA-A29, B56 항원과 panmyeloid 면역 표현형인 CD33, CD15와의 연관성이 관찰되었고, A2-B48, Cw7-B44 일배체형과 myeloid 면역 표현형인 CD34, CD33, CD11c와의 연관성이 관찰되었다. 결론:AML과 MDS는 HLA와 연관성 있는 질환이며, AML과 MDS군의 발생 및 AML군에서 백혈병 세포의 분화 단계를 예측하기 위해 본 연구 자료가 유용할 것으로 생각된다. Background:The Major Histocompatibility Complex (MHC) has been widely studied in the field of transplantation immunology, since initially defined as transplantation antigen in mouse in 1936. Although associations have been demonstrated between many autoimmune diseases and HLA in human, the studies of HLA association with human leukemia are rare, and have too small sample size to get a significant result. We tried to invesgate the association of AML and MDS with HLA, and the association of FAB, immunological subgroup with HLA in AML group by using appropriate statistical methods. The purpose of this study is to elucidate the HLA role in the development of AML and MDS, and find any HLA antigens or haplotypes which can predict the development of AML or MDS. Methods: The subject of this study was 415 patients with AML and 62 patients with MDS who admitted in St. Mary's hospital between 1991. 1. and 1998. 7., and were typed for HLA class I, II antigens by using serological method of NIH standard microlymphocytotoxicity. We calculated the HLA antigen, gene and 2-locus haplotype frequencies in AML and MDS group and FAB, immunological subgroup, calculated relative risk by Haldane's method by comparing HLA antigen frequencies in disease group with those in normal control population. The Chi-Square method or Fisher's exact test was used to assess the significance of the differencies in the antigen and haplogtype distributions in the control vs disease population. Results: 1)The frequencies of Cw8 were severely decreased in AML and MDS population with relative risk of 0.10, 0.22, and revealed high statistical significance with p-values less than 0.001, suggesting strong Cw8 association with AML and MDS. This means Cw8 may be a gene which resists to the development of AML and MDS, or may be linked to other recessive gene, Cw8 may be a gene which encodes increased immune responses to exogenous antigens such as leukemia virus, or may be linked to other immune response gene, otherwise, Cw8 may be an antigen which has a key role in immune surveillance to the development of AML or MDS. 2) Blank gene frequencies were increased in AML and MDS group, and this phenomenon was prominent in DR locus, showing 11.6% and 7.0% of DR-blank gene frequencies in AML and MDS. This suggests increased HLA homozygosity or increased undetected antigens due to loss or modification of HLA alleles in AML and MDS. 3) The associations of A2-B61, Cw8-B61, B62-DR11 haplotypes with AML, and A30-B14, Cw3-B51, B62-DR14, B35-DR9 haplotypes with MDS were observed. 4) According to FAB classification in AML, highly significant HLA-A33 antigen association with AML-M3 and A2-B51 haplotype association with AML-M1 were observed. 5) According to immunological classification in AML, HLA-A29, B56 each antigen association with CD33, CD15, panmyeloid immunophenotypes and A2-B48, Cw7-B44 each haplotype association with CD34, CD33, CD11c, myeloid immunophenotypes were observed. Conclusion:AML and MDS is a disease associated with HLA, and this study will be valuable in predicting the development of AML and MDS, differentiation stages of leukemic cells in AML.

Factors Associated with Removal of Impactted Fishbone in Children, Suspected Ingestion

Lim, Chun Woo,Park, Min Hwan,Do, Hyun Jeong,Yeom, Jung-Sook,Park, Ji Sook,Park, Eun Sil,Seo, Ji Hyun,Park, Jung Je,Lim, Jae Young,Park, Chan Hoo,Woo, Hyang-Ok,Youn, Hee-Shang The Korean Society of Pediatric Gastroenterology 2016 Pediatric gastroenterology, hepatology & nutrition Vol.19 No.3

Purpose: The management and clinical course in pediatric patients who had ingested foreign body were investigated retrospectively to evaluate the frequency and factor associated with successful removal of fishbone foreign body. Methods: Based on the medical records of patients younger than 15 years old who visited emergency room because of foreign body ingestion from January 1999 to December 2012, the authors reviewed clinical characteristics including type of ingested foreign bodies, time to visits, managements and complications. Results: Fishbone (50.1%) was the most common ingested foreign body in children. Among 416 patients with ingested fishbone, 245 (58.9%) were identified and removed using laryngoscope, rigid or flexible endoscope from pharynx or upper esophagus by otolaryngologists and pediatric gastroenterologists. The kind of ingested fish bone in children was diverse. The mean age of identified and removed fishbone group was 7.39 years old, and higher than that of unidentified fishbone group (5.81 years old, p<0.001). Identified and removed fishbone group had shorter time until hospital visit than the unidentified fishbone group (2.03 vs. 6.47 hours, p<0.001). No complication due to ingested fishbone or procedure occurred. Conclusion: Older age and shorter time from accident to hospital visit were the different factors between success and failure on removal of ingested fish bone in children.