METTL3 promotes adriamycin resistance in MCF-7 breast cancer cells by accelerating pri-microRNA-221-3p maturation in a m6A-dependent manner

Pan Xiaoping,Hong Xiaolv,Li Sumei,Meng Ping,Xiao Feng 생화학분자생물학회 2021 Experimental and molecular medicine Vol.53 No.-

Breast cancer (BC) is the most prevalent malignant neoplasm among women and is the fifth most common cause of cancer-associated death worldwide. Acquired chemoresistance driven by genetic and epigenetic alterations is a significant clinical challenge in treating BC. However, the mechanism of BC cell resistance to adriamycin (ADR) remains to be elucidated. In this study, we identified the methyltransferase-like 3/microRNA-221-3p/homeodomain-interacting protein kinase 2/Che-1 (METTL3/miR-221-3p/HIPK2/Che-1) axis as a novel signaling event that may be responsible for resistance of BC cells to ADR. A dual-luciferase reporter gene assay was employed to test the presence of miR-221-3p binding sites in the 3′UTR of HIPK2. Drug resistance was evaluated by immunoblotting multidrug resistance protein 1 (MDR1) and breast cancer resistance protein (BCRP). Cultured ADR-resistant MCF-7 cells were assayed for their half maximal inhibitory concentration (IC50) values and apoptosis using an MTT assay and Annexin V-FITC/PI-labeled flow cytometry, and the cells were then xenografted into nude mice. METTL3 knockdown was shown to reduce the expression of miR-221-3p by reducing pri-miR-221-3p m6A mRNA methylation, thereby reducing the IC50 value of ADR-resistant MCF-7 cells, reducing the expression of MDR1 and BCRP, and inducing apoptosis. Mechanistically, miR-221-3p was demonstrated to negatively regulate HIPK2 and upregulate its direct target Che-1, thus leading to enhanced drug resistance in ADR-resistant MCF-7 cells. In vitro results were reproduced in nude mice xenografted with ADR-resistant MCF-7 cells. Our work elucidates an epigenetic mechanism of acquired chemoresistance in BC, in support of the METTL3/miR-221-3p/HIPK2/Che-1 axis as a therapeutic target for the improvement of chemotherapy.

Evaluation of HP300 Soybean Protein in Starter pig Diets

Zhu, Xiaoping,Li, Defa,Qiao, Shiyan,Xiao, Changting,Qiao, Qingyan,Ji, Cheng Asian Australasian Association of Animal Productio 1998 Animal Bioscience Vol.11 No.2

One growth trial and one digestibility trial were conducted to evaluate the nutritional value of HP300, a commercially processed soybean meal product for weanling pigs. Dried whey, fish meal and/or full fat extruded soybeans (FFES) as well as portions of soybean meal (SBM) were replaced with HP300 in weanling pig diets. The objectives were to investigate the effects of HP300 on growth performance, digestibility, ileal amino acid digestibility and blood amino acid concentration in weanling pigs. One hundred and twenty crossbred $(Duroc{\times}Beijing\;Black{\times}Landrace)$ pigs weaned at 28 days of age were used in the growth trial. The pigs were randomly allocated to five treatments, with three pens per treatment and eight pigs per pen. The trial duration was 28 days. The control (CTRL) diet contained no HP300; in treatments 2, 3 and 4, dried whey and fish meal were replaced by 3.0%, 7.5% and 10.5% HP300; in treatment 5, full fat extruded soybeans were replaced by 10.5% HP300 plus soybean oil to attain the same metabolic energy content as FFES. Five T-cannulated barrows were used in a digestibility trial with a $5{\times}5$ Latin square design to determine nitrogen retention and amino acid ileal digestibility of HP300 used alone or mixed with other ingredients. The results indicated that replacement of dried whey, fish meal, full fat extruded soybeans and a part of the soybean meal with HP300 in piglet diets improved average daily gain and feed conversion ratio (p < 0.05). There was a trend toward improved DM, crude protein and amino acid ileal digestibilities and improved protein and amino acid ileal digestibilities and improved protein net availability with the use of HP300 in swine diets.

Partial gasification of coal in a fluidized bed reactor: Comparison of a laboratory and pilot scale reactors

Rui Xiao,Laihong Shen,Mingyao Zhang,Baosheng Jin,Yuanquan Xiong,Yufeng Duan,Zhaoping Zhong,Xiaoping Chen,Yaji Huang,Hongcang Zhou 한국화학공학회 2007 Korean Journal of Chemical Engineering Vol.24 No.1

New Construction Method for Quaternary Aperiodic, Periodic, and Z-Complementary Sequence Sets

Fanxin Zeng,Xiaoping Zeng,Zhenyu Zhang,Xiangyong Zeng,Guixin Xuan,Lingna Xiao 한국통신학회 2012 Journal of communications and networks Vol.14 No.3

Based on the known binary sequence sets and Gray mapping,a new method for constructing quaternary sequence sets is presented and the resulting sequence sets’ properties are investigated. As three direct applications of the proposed method, when we choose the binary aperiodic, periodic, and Z-complementary sequence sets as the known binary sequence sets, the resultant quaternary sequence sets are the quaternary aperiodic, periodic, and Z-complementary sequence sets, respectively. In comparison with themethod proposed by Jang et al., the new method can cope with either both the aperiodic and periodic cases or both even and odd lengths of sub-sequences, whereas the former is only fit for the periodic case with even length of sub-sequences. As a consequence,by both our and Jang et al.’s methods, an arbitrary binary aperiodic,periodic, or Z-complementary sequence set can be transformed into a quaternary one no matter its length of sub-sequences is odd or even. Finally, a table on the existing quaternary periodic complementary sequence sets is given as well.

16-QAM Periodic Complementary Sequence Mates Based on Interleaving Technique and Quadriphase Periodic Complementary Sequence Mates

Fanxin Zeng,Xiaoping Zeng,Lingna Xiao,Zhenyu Zhang,Guixin Xuan 한국통신학회 2013 Journal of communications and networks Vol.15 No.6

Based on an interleaving technique and quadriphase periodiccomplementary sequence (CS) mates, this paper presents amethod for constructing a family of 16-quadrature amplitudemodulation(QAM) periodic CS mates. The resulting mates arise fromthe conversion of quadriphase periodic CS mates, and the period ofthe former is twice as long as that of the latter. In addition, basedon the existing binary periodic CS mates, a table on the existenceof the proposed 16-QAM periodic CS mates is given. Furthermore,the proposed method can also transform a mutually orthogonal(MO) quadriphase CS set into an MO 16-QAM CS set. Finally,three examples are given to demonstrate the validity of the proposedmethod.

Tuber borchii Shapes the Ectomycorrhizosphere Microbial Communities of Corylus avellana

( Xiaolin Li ),( Xiaoping Zhang ),( Mei Yang ),( Lijuan Yan ),( Zongjing Kang ),( Yujun Xiao ),( Ping Tang ),( Lei Ye ),( Bo Zhang ),( Jie Zou ),( Chengyi Liu ) 한국균학회 2019 Mycobiology Vol.47 No.2

In this study, eight-month-old ectomycorrhizae of Tuber borchii with Corylus avellana were synthesized to explore the influence of T. borchii colonization on the soil properties and the microbial communities associated with C. avellana during the early symbiotic stage. The results showed that the bacterial richness and diversity in the ectomycorrhizae were significantly higher than those in the control roots, whereas the fungal diversity was not changed in response to T. borchii colonization. Tuber was the dominant taxon (82.97%) in ectomycorrhizae. Some pathogenic fungi, including Ilyonectria and Podospora, and other competitive mycorrhizal fungi, such as Hymenochaete, had significantly lower abundance in the T. borchii inoculation treatment. It was found that the ectomycorrhizae of C. avellana contained some more abundant bacterial genera (e.g., Rhizobium, Pedomicrobium, Ilumatobacter, Streptomyces, and Geobacillus) and fungal genera (e.g., Trechispora and Humicola) than the control roots. The properties of rhizosphere soils were also changed by T. borchii colonization, like available nitrogen, available phosphorus and exchangeable magnesium, which indicated a feedback effect of mycorrhizal synthesis on soil properties. Overall, this work highlighted the interactions between the symbionts and the microbes present in the host, which shed light on our understanding of the ecological functions of T. borchii and facilitate its commercial cultivation.

New Construction Method for Quaternary Aperiodic, Periodic, and Z-Complementary Sequence Sets

Zeng, Fanxin,Zeng, Xiaoping,Zhang, Zhenyu,Zeng, Xiangyong,Xuan, Guixin,Xiao, Lingna The Korea Institute of Information and Commucation 2012 Journal of communications and networks Vol.14 No.3

Based on the known binary sequence sets and Gray mapping, a new method for constructing quaternary sequence sets is presented and the resulting sequence sets' properties are investigated. As three direct applications of the proposed method, when we choose the binary aperiodic, periodic, and Z-complementary sequence sets as the known binary sequence sets, the resultant quaternary sequence sets are the quaternary aperiodic, periodic, and Z-complementary sequence sets, respectively. In comparison with themethod proposed by Jang et al., the new method can cope with either both the aperiodic and periodic cases or both even and odd lengths of sub-sequences, whereas the former is only fit for the periodic case with even length of sub-sequences. As a consequence, by both our and Jang et al.'s methods, an arbitrary binary aperiodic, periodic, or Z-complementary sequence set can be transformed into a quaternary one no matter its length of sub-sequences is odd or even. Finally, a table on the existing quaternary periodic complementary sequence sets is given as well.

16-QAM Periodic Complementary Sequence Mates Based on Interleaving Technique and Quadriphase Periodic Complementary Sequence Mates

Zeng, Fanxin,Zeng, Xiaoping,Xiao, Lingna,Zhang, Zhenyu,Xuan, Guixin The Korea Institute of Information and Commucation 2013 Journal of communications and networks Vol.15 No.6

Based on an interleaving technique and quadriphase periodic complementary sequence (CS) mates, this paper presents a method for constructing a family of 16-quadrature amplitude modulation (QAM) periodic CS mates. The resulting mates arise from the conversion of quadriphase periodic CS mates, and the period of the former is twice as long as that of the latter. In addition, based on the existing binary periodic CS mates, a table on the existence of the proposed 16-QAM periodic CS mates is given. Furthermore, the proposed method can also transform a mutually orthogonal (MO) quadriphase CS set into an MO 16-QAM CS set. Finally, three examples are given to demonstrate the validity of the proposed method.

Co–Fe Bimetal Phosphate Composite Loaded on Reduced Graphene Oxide for Oxygen Evolution

Guoxing Zhu,Xulan Xie,Lisong Xiao,Xiaoyun Li,Xiaoping Shen,Yuanjun Liu 성균관대학교(자연과학캠퍼스) 성균나노과학기술원 2019 NANO Vol.14 No.1

Development of high-performance nonprecious metal-based catalysts for oxygen evolution reaction (OER) is crucial to improve the efficiency of water electrolysis and photoelectrochemical water splitting for harvesting and storage of solar energy. Herein, Co–Fe phosphates and their composites with reduced graphene oxide (rGO) were prepared by a simple hydrothermal method, which then acted as oxygen evolution reaction catalysts. In 1.0 M KOH aqueous solution, the as-obtained optimal composite, Co–Fe phosphate/rGO, can catalyze oxygen evolution reaction with a very sharp onset potential and a small over-potential of 338 mV to achieve a current density of 10 mA cm -2. It was found that in these Co–Fe phosphates, the optimal Co:Fe ratio is 0.75:0.25. The excellent electrocatalytic performance of the Co–Fe phosphate/rGO composite would benefit from the synergistic effects between Fe and Co species, as well as rGO substrate providing conductive channels. The formed Co–Fe phosphate/rGO electrocatalysts can be the promising replacement of precious metal-based catalysts for more practical and cost-efficient water splitting.

Effects of Long-Term Fertilizer Practices on Rhizosphere Soil Autotrophic CO₂-Fixing Bacteria under Double Rice Ecosystem in Southern China

( Haiming Tang ),( Li Wen ),( Lihong Shi ),( Chao Li ),( Kaikai Cheng ),( Weiyan Li ),( Xiaoping Xiao ) 한국미생물생명공학회 2022 Journal of microbiology and biotechnology Vol.32 No.10

Soil autotrophic bacterial communities play a significant role in the soil carbon (C) cycle in paddy fields, but little is known about how rhizosphere soil microorganisms respond to different long-term (35 years) fertilization practices under double rice cropping ecosystems in southern China. Here, we investigated the variation characteristics of rhizosphere soil RubisCO gene cbbL in the double rice ecosystems of in southern China where such fertilization practices are used. For this experiment we set up the following fertilizer regime: without any fertilizer input as a control (CK), inorganic fertilizer (MF), straw returning (RF), and organic and inorganic fertilizer (OM). We found that abundances of cbbL, 16S rRNA genes and RubisCO activity in rhizosphere soil with OM, RF and MF treatments were significantly higher than that of CK treatment. The abundances of cbbL and 16S rRNA genes in rhizosphere soil with OM treatment were 5.46 and 3.64 times higher than that of CK treatment, respectively. Rhizosphere soil RubisCO activity with OM and RF treatments increased by 50.56 and 45.22%, compared to CK treatment. Shannon and Chao1 indices for rhizosphere soil cbbL libraries with RF and OM treatments increased by 44.28, 28.56, 29.60, and 23.13% compared to CK treatment. Rhizosphere soil cbbL sequences with MF, RF and OM treatments mainly belonged to Variovorax paradoxus, uncultured proteobacterium, Ralstonia pickettii, Thermononospora curvata, and Azoarcus sp.KH33C. Meanwhile, cbbL-carrying bacterial composition was obviously influenced by soil bulk density, rhizosphere soil dissolved organic C, soil organic C, and microbial biomass C contents. Fertilizer practices were the principal factor influencing rhizosphere soil cbbL-carrying bacterial communities. These results showed that rhizosphere soil autotrophic bacterial communities were significantly changed under conditions of different long-term fertilization practices Therefore, increasing rhizosphere soil autotrophic bacteria community with crop residue and organic manure practices was found to be beneficial for management of double rice ecosystems in southern China.