Inhibitory Effects of Syk Transfection on Lung Cancer Cell Invasion

Peng, Chuan-Liang,Zhang, Ying,Sun, Qi-Feng,Zhao, Yun-Peng,Hao, Ying-Tao,Zhao, Xiao-Gang,Cong, Bo Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.5

Objective: Spleen tyrosine kinase (Syk) is closely related to tumor invasion and metastasis, and has been shown to have potential inhibitory effects in tumors. In this study, we constructed a eukaryotic expression vector for Syk and analyzed its effects on invasive ability of the A549 non-small cell lung cancer cell line in vitro. Methods: A fragment of Syk was obtained by RT-PCR from human lung cancer cells and cloned into the expression vector pLNCXSyk. After restriction endonuclease digestion, PCR and DNA sequencing confirmation, the recombinant Syk expression plasmid was transfected into A549 human lung cancer cells using lipofectamine protocols. After selection, the cells stably expressed Syk. Detection of Syk expression of the cells by RT-PCR, and invasive ability were examined. Results: The eukaryotic expression plamid pLNCXSyk was constructed and expressed stably in the A549 human lung cancer cells. The RT-PCR results showed that Syk mRNA expression was upregulated significantly (P<0.05). Lower invasion through a basal membrane were apparent after transfection (P<0.05). Conclusions: A eukaryotic expression plasmid to cause Syk expression in lung cancer cells can obviously inhibit their invasive ability in vitro.

Synthesis and Biological Evaluation of Furan-chalcone Derivatives as Protein Tyrosine Phosphatase Inhibitors

Sun, Liang-Peng,Jiang, Zhe,Gao, Li-Xin,Sheng, Li,Quan, Ying-Chun,Li, Jia,Piao, Hu-Ri Korean Chemical Society 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

Synthesis and Biological Evaluation of Furan-chalcone Derivatives as Protein Tyrosine Phosphatase Inhibitors

Liang-Peng Sun,Zhe Jiang,Li-Xin Gao,Li Sheng,Ying-Chun Quan,Jia Li,Hu-Ri Piao 대한화학회 2013 Bulletin of the Korean Chemical Society Vol.34 No.4

Observation of the topological surface state in the nonsymmorphic topological insulator KHgSb

Liang, A. J.,Jiang, J.,Wang, M. X.,Sun, Y.,Kumar, N.,Shekhar, C.,Chen, C.,Peng, H.,Wang, C. W.,Xu, X.,Yang, H. F.,Cui, S. T.,Hong, G. H.,Xia, Y.-Y.,Mo, S.-K.,Gao, Q.,Zhou, X. J.,Yang, L. X.,Felser, C. American Physical Society 2017 Physical review. B Vol.96 No.16

<P>Topological insulators represent unusual topological quantum states, typically with gapped bulk band structure but gapless surface Dirac fermions protected by time-reversal symmetry. Recently, a distinct kind of topological insulator resulting from nonsymmorphic crystalline symmetry was proposed in the KHgX (X = As, Sb, Bi) compounds. Unlike regular topological crystalline insulators, the nonsymmorphic glide-reflection symmetry in KHgX guarantees the appearance of an exotic surface fermion with hourglass shape dispersion (where two pairs of branches switch their partners) residing on its (010) side surface, contrasting to the usual two-dimensional Dirac fermion form. Here, by using high-resolution angle-resolved photoemission spectroscopy, we systematically investigate the electronic structures of KHgSb on both (001) and (010) surfaces and reveal the unique in-gap surface states on the (010) surface with delicate dispersion consistent with the 'hourglass Fermion' recently proposed. Our experiment strongly supports that KHgSb is a nonsymmorphic topological crystalline insulator with hourglass fermions, which serves as an important step to the discovery of unique topological quantum materials and exotic fermions protected by nonsymmorphic crystalline symmetry.</P>

Control System Design of Anti-rolling Tank Swing Bench Using BP Neural Network PID Based on LabVIEW

Lihua Liang,Mingxiao Sun,Songtao Zhang,Yu Wen,Peng Zhao,Jia Yuan 보안공학연구지원센터 2015 International Journal of Smart Home Vol.9 No.6

The anti-rolling tank swing bench is a typical hydraulic position servo system. Its function is to simulate the ship roll motion and to verify the performance of the anti-rolling tank. Generally, the PID control method can achieve an ideal control effect. But the uncertainties, such as the couplings and the nonlinearty of the swing bench, make it difficult to establish the precise mathematical model of system. During experiments, the different disturbance torques are generated according to the different dimension scales of the anti-rolling tanks. Changes of the natural characteristics of the system are caused by changes of load, and the fixed parameters of PID controller are difficult to continue to ensure a good control effect. In order to reduce the influences caused by the factors mentioned above, a BP neural network PID control system based on virtual instrument LabVIEW is designed. By simulating the responses of the system in real time control, the verification tests using actual system are avoided. Finally, the experiments of swing bench are carried out. The results show that the controller can ensure the control accuracy and stability. The parameters tuning process efficiency is improved. The operation is convenient. The data acquisition process is simple and practical.

Spot 42 RNA regulates putrescine catabolism in Escherichia coli by controlling the expression of puuE at the post-transcription level

Xin Sun,Ruyan Li,Guochen Wan,Wanli Peng,Shuangjun Lin,Zixin Deng,Rubing Liang 한국미생물학회 2021 The journal of microbiology Vol.59 No.2

Putrescine, a typical polyamine compound important for cell growth and stress resistance, can be utilized as an energy source. However, the regulation of its catabolism is unclear. Here the small RNA (sRNA) Spot 42, an essential regulator of carbon catabolite repression (CCR), was confirmed to participate in the post-transcriptional regulation of putrescine catabolism in Escherichia coli. Its encoding gene spf exclusively exists in the γ-proteobacteria and contains specific binding sites to the 5 -untranslated regions of the puuE gene, which encodes transaminase in the glutamylated putrescine pathway of putrescine catabolism converting γ-aminobutyrate (GABA) into succinate semialdehyde (SSA). The transcription of the spf gene was induced by glucose, inhibited by putrescine, and unaffected by PuuR, the repressor of puu genes. Excess Spot 42 repressed the expression of PuuE significantly in an antisense mechanism through the direct and specific base-pairing between the 51–57 nt of Spot 42 and the 5 - UTR of puuE. Interestingly, Spot 42 mainly influenced the stability of the puuCBE transcript. This work revealed the regulatory role of Spot 42 in putrescine catabolism, in the switch between favorable and non-favorable carbon source utilization, and in the balance of metabolism of carbon and nitrogen sources.

Ellipsometric Study of the Optical Properties of Silicon-Based Si:SiO2 Composite Thin Films under Different Annealing Temperatures

Bin Sun,Yu-Xiang Zheng,Cong-Hui Xu,Liang-Yao Chen,Peng Zhou,Yue-Rui Chen,Yu-Fei Kong 한국물리학회 2006 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.49 No.5I

In our work, silicon and silica composite .lms were prepared by using a magnetron sputtering method, and the samples were annealed in the temperature range between 200 ±C and 600 ±C. After annealing, the samples were investigated by spectroscopic ellipsometry (SE), and the SE spectra were measured in the range of 1.5 eV ≫ 4.5 eV with a dual-rotating-element ellipsometer. In order to investigate the microstructure information of the .lm by .tting the SE spectra using the eective- medium approximation (EMA), we modeled the whole .lm by using a multilayer optical model including a surface oxide layer, a composite layer, and substrate layer. We used dierent mixtures to describe the composite layer, and .nally we found that a mixture of amorphous silicon (a-Si), polycrystalline silicon (p-Si), and silicon dioxide had the best .t to the SE spectra. Whereafter, we studied the dependences of the surface roughness, the layer structure, and the composition on the dierent annealing temperatures and found that with increasing annealing temperature, the surface oxide layer grew in thickness, the silicon dioxide maintained its volume fraction, and a-Si transferred to p-Si.

VvMYBA6 in the promotion of anthocyanin biosynthesis and salt tolerance in transgenic Arabidopsis

Miao Sun,Xin-Xin Feng,Jian-Jie Ga,Ri-He Peng,Quan-Hong Yao,Liang-Ju Wang 한국식물생명공학회 2017 Plant biotechnology reports Vol.11 No.5

Transcription factor MYB is a critical factor involved in anthocyanin biosynthesis and abiotic stress resistance of plants. This paper reports the functions of codon-optimized transcription factor VvMYBA6 expressed in transgenic Arabidopsis plants. VvMYBA6 overexpression resulted in significant increase of anthocyanin content, which also enhanced salt and mannitol tolerance in transgenic Arabidopsis plants. Besides, real-time quantitative PCR (qRT-PCR) analysis showed that overexpression of VvMYBA6 promoted the expression level of genes involved in anthocyanin biosynthesis, abscisic acid (ABA) synthesis, proline synthesis, stress responses and ROS scavenging under salt and mannitol stress. According to further analysis under salt and mannitol stress, overexpression of VvMYBA6 significantly increased ABA and proline content, activities of superoxide dismutase (SOD), peroxidase (POD) and catalase (CAT), as well as reduced the content of H2O2 and malonaldehyde (MDA). The results demonstrated that the overexpression of VvMYBA6 enhanced salt and mannitol tolerance based on increasing synthetic levels of anthocyanin and ABA in transgenic Arabidopsis plants. Therefore, the overexpression of VvMYBA6 could be widely used in promoting salt tolerance in plants.

Effect of Saccharomyces cerevisiae boulardii on sows’ farrowing duration and reproductive performance, and weanling piglets’ performance and IgG concentration

( Haiqing Sun ),( Fernando Bravo De Laguna ),( Shuai Wang ),( Fengju Liu ),( Liang Shi ),( Haidi Jiang ),( Xiaoxia Hu ),( Peng Qin ),( Jiajian Tan ) 한국축산학회 2022 한국축산학회지 Vol.64 No.1

We studied the effects of Saccharomyces cerevisiae boulardii CNCM I-1079 (LSB) supplemented to lactating sows on reproductive traits and farrowing duration and to piglets from day 7 of life on post-weaning performance and IgG concentration. Ninety-six Landrace × Yorkshire sows started the trial 5 days before the expected farrowing date. Sows were distributed into 2 groups according to parity number and backfat thickness: control (CON: regular lactation diet) and LSB (CON + LSB at 2 × 10⁹ colony forming units [CFU]/kg of feed). Seven days after birth, litters were randomly selected from each group and supplemented creep feed with or without LSB at 2 × 10⁹ CFU/kg. At weaning, piglets from CON sows were shifted to a commercial farm and allocated to 14 pens in groups of 25 piglets/pen according to the creep feed supplemented during lactation. Piglets followed a 3-phase feeding program: creep, pre-starter and starter, with or without LSB at 2 × 10⁹ CFU/kg LSB in creep and pre-starter, and 1 × 10⁹ CFU/kg LSB in starter. The piglets were vaccinated against classical swine fever on days 41 and 72 of life. One day before each vaccination and at the end of the trial, blood samples were collected from 15 randomly selected piglets per treatment and assessed for total IgG. Supplemented sows with non-supplemented litters displayed the lowest backfat thickness loss during lactation (p < 0.05). The LSB supplementation shortened farrowing duration (p < 0.05) and increased feed intake (p < 0.05) during the first week of lactation. The LSB-fed piglets were heavier at the end of creep (p < 0.05), pre-starter (p < 0.05), and the trial (p < 0.05); grew faster during creep (p < 0.05), starter (p < 0.05), and overall (p < 0.05); and displayed an improved feed conversion ratio during creep (p < 0.05). Total IgG content was higher at days 40 (p < 0.05) and 71 (p < 0.05) in LSB-fed piglets. We conclude that supplementing sows with Saccharomyces cerevisiae boulardii CNCM I-1079 from late gestation until weaning shortens farrowing duration, increases feed intake, and minimizes backfat losses during lactation. When supplemented to piglet diet, post-weaning performance is improved. This improvement observed could be linked to a better immune status, as suggested by the higher IgG.

Rapid separation and identification of 31 major saponins in Shizhu ginseng by ultra-high performance liquid chromatography-electron spray ionization-MS/MS

Ting-Ting Sun,Xin-Lei Liang,He-Yun Zhu,Xu-Ling Peng,Xing-Jie Guo,Long-Shan Zhao 고려인삼학회 2016 Journal of Ginseng Research Vol.40 No.3

Background: Among the various ginseng strains, Shizhu ginseng is endemic to China, mainly distributed in Kuandian Manchu Autonomous County (Liaoning Province, China); however, not much is known about the compounds (especially saponins) in Shizhu ginseng. Methods: A rapid, sensitive, and reliable ultra-high performance liquid chromatography coupled with MS/MS (UHPLCeMS/MS) method was developed to separate and identify saponins in Shizhu ginseng. Results: The separation was carried out on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 ㎛) with acetonitrile and 0.1% formic acid aqueous solution as the mobile phase under a gradient elution at 40℃. The detection was performed on a Micromass Quattro Micro API mass spectrometer equipped with electrospray ionization source in both positive and negative modes. Under the optimized conditions, a total of 31 saponins were identified or tentatively characterized by comparing retention time and MS data with related literatures and reference substances. Conclusion: The developed UHPLCeMS/MS method was suitable for identifying and characterizing the chemical constituents in Shizhu ginseng, which provided a helpful chemical basis for further research on Shizhu ginseng.