The Production and Cytological Analysis of Brassica napus-B Genome Chromosome Monosomic Addition Lines and Their Hybrids

Mao Teng Li,Jun Xiang,Jian Min Liu,Long Jiang Yu,Dian Rong Li 한국유전학회 2008 Genes & Genomics Vol.30 No.2

The Brassica napus-B genome monosomic addition lines (MALs) (AACC + B`, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus-B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19II+1I, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20II and 19II+2I that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes.

Tumor-Derived Transforming Growth Factor-β is Critical for Tumor Progression and Evasion from Immune Surveillance

Li, Zheng,Zhang, Li-Juan,Zhang, Hong-Ru,Tian, Gao-Fei,Tian, Jun,Mao, Xiao-Li,Jia, Zheng-Hu,Meng, Zi-Yu,Zhao, Li-Qing,Yin, Zhi-Nan,Wu, Zhen-Zhou Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.13

Tumors have evolved numerous mechanisms by which they can escape from immune surveillance. One of these is to produce immunosuppressive cytokines. Transforming growth factor-${\beta}$(TGF-${\beta}$) is a pleiotropic cytokine with a crucial function in mediating immune suppression, especially in the tumor microenvironment. TGF-${\beta}$ produced by T cells has been demonstrated as an important factor for suppressing antitumor immune responses, but the role of tumor-derived TGF-${\beta}$ in this process is poorly understood. In this study, we demonstrated that knockdown of tumor-derived TGF-${\beta}$ using shRNA resulted in dramatically reduced tumor size, slowing tumor formation, prolonging survival rate of tumor-bearing mice and inhibiting metastasis. We revealed possible underlying mechanisms as reducing the number of myeloid-derived suppressor cells (MDSC) and $CD4^+Foxp3^+$ Treg cells, and consequently enhanced IFN-${\gamma}$ production by CTLs. Knockdown of tumor-derived TGF-${\beta}$ also significantly reduced the conversion of na$\ddot{i}$ve $CD4^+$ T cells into Treg cells in vitro. Finally, we found that knockdown of TGF-${\beta}$ suppressed cell migration, but did not change the proliferation and apoptosis of tumor cells in vitro. In summary, our study provided evidence that tumor-derived TGF-${\beta}$ is a critical factor for tumor progression and evasion of immune surveillance, and blocking tumor-derived TGF-${\beta}$ may serve as a potential therapeutic approach for cancer.

A κ-Carrageenase from a Newly Isolated Pseudoalteromonas-like Bacterium, WZUC10

Mao-hong Zhou,Jian-she Ma,Jun Li,Hai-ren Ye,Ke-xin Huang,Xiao-wei Zhao 한국생물공학회 2008 Biotechnology and Bioprocess Engineering Vol.13 No.5

A bacterial strain able to produce k-carrageenase, designated WZUC10, was isolated from a live specimen of the red alga Plocamium telfairiae collected in the East China Sea. The phylogenetic evidence and phenotypic features indicate that this strain belongs to the genus Pseudoalteromonas. WZUC10 requires NaCl for growth and k-carrageenan to induce k-carrageenase synthesis; galactose and lactose do not induce it. The optimal growth temperature is 23~27°C. The secreted enzyme, which has a molecular mass of 45 kDa, breaks down k-carrageenan into k-neocarratetraose sulfate and larger oli-gosaccharides with a repeating β-D-Galp4S-(1→4)-α-D-AnGalp structure, but cannot degrade k-neocarratetraose sulfate or k-neocarrahexaose sulfate into k-neocarrabiose sulfate. The enzyme retains 90% of its activity after 2 h at 40°C and is com-pletely inactivated after 7.5 min at 70°C. The enzyme’s optimal temperature is 30°C and its optimal pH is 7.5. The enzyme-catalyzed reaction follows Michaelis-Menten kinetics, with the Michaelis constant (Km) and the turnover number (k) being 0.015 mM and 125 s-¹, respectively. WZUC10 produces 50 U/mL k-carrageenase after cultivation at 25°C for 35 h on a me-dium containing 80 g/L glucose, 5 g/L corn steep liquor, 3 g/L k-carrageenan, and 15 g/L NaCl. k-Neocarratetraose sulfate was prepared simply with precipitation by ethanol:water (5:1, v/v).

Controller Backup and Replication for Reliable Multi-domain SDN

( Junli Mao ),( Lishui Chen ),( Jiacong Li ),( Yi Ge ) 한국인터넷정보학회 2020 KSII Transactions on Internet and Information Syst Vol.14 No.12

Software defined networking (SDN) is considered to be one of the most promising paradigms in the future. To solve the scalability and performance problem that a single and centralized controller suffers from, the distributed multi-controller architecture is adopted, thus forms multi-domain SDN. In a multi-domain SDN network, it is of great importance to ensure a reliable control plane. In this paper, we focus on the reliability problem of multi-domain SDN against controller failure from perspectives of backup controller deployment and controller replication. We firstly propose a placement algorithm for backup controllers, which considers both the reliability and the cost factors. Then a controller replication mechanism based on shared data storage is proposed to solve the inconsistency between the active and standby controllers. We also propose a shared data storage layout method that considers both reliability and performance. Besides, a fault recovery and repair process is designed based on the controller backup and shared data storage mechanism. Simulations show that our approach can recover and repair controller failure. Evaluation results also show that the proposed backup controller placement approach is more effective than other methods.

A Genetic Variant in MiR-146a Modifies Digestive System Cancer Risk: a Meta-analysis

Li, Ying-Jun,Zhang, Zhen-Yu,Mao, Ying-Ying,Jin, Ming-Juan,Jing, Fang-Yuan,Ye, Zhen-Hua,Chen, Kun Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.1

MicroRNAs (miRNAs) negatively regulate gene expression and act as tumor suppressors or oncogenes in oncogenesis. The association between a single nucleotide polymorphism (SNP) in miR-146a rs2910164 and susceptibility to digestive system cancers was inconsistent in previous studies. In this study, we conducted a literature search of PubMed to identify all relevant studies published before August 31, 2013. A total of 21 independent case-control studies were included in this updated meta-analysis with 9,558 cases and 10,614 controls. We found that the miR-146a rs2910164 polymorphism was significantly associated with decreased risk of digestive system cancers in an allele model (OR=0.90, 95%CI 0.87-0.94), homozygote model (OR=0.84, 95%CI 0.77-0.91), dominant model (OR=0.90, 95%CI 0.84-0.96), and recessive model (OR=0.85, 95%CI 0.79-0.91), while in a heterozygous model (OR = 0.99, 95% CI 0.89-1.11) the association showed marginal significance. Subgroup analysis by cancer site revealed decreased risk in colorectal cancer above allele model (OR=0.90, 95%CI 0.83-0.97) and homozygote model (OR=0.85, 95%CI 0.72-1.00). Similarly, decreased cancer risk was observed when compared with allele model (OR=0.87, 95%CI 0.81-0.93) and recessive model (OR=0.81, 95%CI 0.72-0.90) in gastric cancer. When stratified by ethnicity, genotyping methods and quality score, decreased cancer risks were also observed. This current meta-analysis indicated that miR-146a rs2910164 polymorphism may decrease the susceptibility to digestive system cancers, especially in Asian populations.

Risk Assessment and Mitigation Evaluation for Rockfall Hazards at the Diversion Tunnel Inlet Slope of Jinchuan Hydropower Station by Using Three-dimensional Terrestrial Scanning Technology

Mao-pu Xia,Hai-bo Li,Nan Jiang,Jun-lin Chen,Jia-wen Zhou 대한토목학회 2023 KSCE Journal of Civil Engineering Vol.27 No.1

Rockfall is a major geological disaster that frequently occurs on rock slopes in alpine and gorge regions and poses a significant threat to life and property in areas exposed to rockfall hazards. In this study, a methodology is presented for comprehensive rockfall hazard analysis, including data acquisition, source identification, numerical simulation, quantitative assessment and mitigation evaluation. A high-precision and high-resolution digital terrain model (DTM) is generated based on the point cloud data obtained by terrestrial laser scanning (TLS) and is utilized for rockfall simulation and automatic identification of structural planes. The model parameters are calibrated by three-dimensional (3D) back analysis, and the rockfall motion is simulated based on a probabilistic model and an energy dissipation model. Rockfall mitigation under passive protection is evaluated according to the rockfall interception ratio (RIR), hazard reduction rate (HRR) and statistics of the rockfall characteristics and hazards within the rockfall threat area. Under the influence of construction excavation, the diversion tunnel inlet slope of Jinchuan Hydropower Station has suffered from a series of rockfall events and potential overall instability, which has become a great threat to the construction personnel, equipment and vehicles below the slope. Thus, the presented methodology is applied to thoroughly study the example case of the rockfall events at the inlet slope of the diversion tunnel of Jinchuan Hydropower Station. The advantages, limitations and notes of each link of the presented methodology are discussed, and the suggestion of strengthening the cracked area with integral active support is proposed.

Identification of ssDNA Aptamers Specific for Anti-neuroexcitation Peptide III and Molecular Modeling Studies: Insights into Structural Interactions

Jun Zhu,Jian Wang,Zhen-Cheng Su,Qin Li,Mao-Sheng Cheng,Jing-Hai Zhang 대한약학회 2008 Archives of Pharmacal Research Vol.31 No.9

Twelve ssDNA aptamers specific for a novel recombinant anti-neuroexcitation peptide (ANEPIII) were identified using the SELEX method from a 79-nucleotide ssDNA pool to purify ANEPIII in a more efficient way. To further understand the binding modes between ssDNA and ANEPIII, fully flexible dinucleotides were docked onto the homology-modeled ANEPIII structure. AutoDocking identified favorable binding sites on ANEPIII for nucleotides, which was valuable for designing more potent ligands.

Expression of HMGB1 and its Clinical Significance in T-cell Lymphoma

Mao, Xing-Jiang,Wang, Geng-Fu,Chen, Zhi-Jun,Wang, Li-Na,Zhang, Jun-Biao,Wang, Hui-Ling Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.11

Objectives: To evaluate the clinical significance of HMGB1 expression in T-cell lymphoma. Methods: Immunohistochemical staining for HMGB1 and survivin was performed with specimens from 120 cases of T-cell lymphoma and 40 cases of reactive lymphoid hyperplasia with antibodies against human HMGB1 and survivin. Results: The expression of HMGB1 and survivin was significantly higher in tissues of T-cell lymphoma than in reactive lymphoid hyperplasia. Positive expression of HMGB1 and survivin was observed in 63.7% (65/102) and 61.8% (63/102) of T-cell lymphoma cases, respectively. While was associated with gender, age, and tumor location, significant correlations with malignancy and clinical stage were observed. Spearman rank correlation analysis revealed that the expression of HMGB1 and survivin was positively correlated in T-cell lymphomas (P<0.01). Conclusions: Expression of HMGB1 and survivin in T-cell lymphomas is significantly associated with malignancy and clinical stage, but not with gender, age and tumor location. Elevated expression of HMGB1 may be an important biomarker for the development and progression of T-cell lymphoma.

Propagation Properties of a Gaussian Beam from a High-NA Optical System in a Magneto-Optical Disk

Li-Chen Jia,Yu-Xiang Zheng,Liang-Yao Chen,Peng-Hui Mao,Rong-Jun Zhang 한국물리학회 2007 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.51 No.II

We have calculated the propagation properties of a Gaussian beam from a high-numerical-aperture (NA) optical system in a magneto-optical (MO) disk by combining an angular spectra analysis and optical matrix method. We obtained the optical and magneto-optical responses, the electric and magnetic field distributions, and the Joule loss profiles for the MO disk on which a Gaussian beam from different NA system was incident and compared the propagation properties of the Gaussian beam with those of a plane wave. The results show that the propagation properties of a Gaussian beam from an optical system with a high NA differ obviously from those of a plane wave, but a Gaussian beam with a larger spot diameter ({\it e.g.}, greater than 100 $\mu$m) can be regarded as a plane wave for the considered disk structure.

Potential Antitumor Activity of SIM-89 in Non-Small Cell Lung Cancer Cells

Jun Pei,Baohui Han,Tianqing Chu,Minhua Shao,Jiajun Teng,Huifang Sha,Aiqing Gu,Rong Li,Jialin Qian,Weifeng Mao,Ying Li 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.3

Purpose: c-Met and its ligand, hepatocyte growth factor (HGF), play a critical role in oncogenesis and metastatic progression. The aim of this study was to identify inhibited enzymogram and to test the antitumor activity of SIM-89 (a c-Met receptor tyrosine kinaseinhibitor) in non-small cell lung cancer. Materials and Methods: Z’-LYTE kinase assay was employed to screen the kinase enzymogram, and mechanism of action (MOA) analysis was used to identify the inhibited kinases. Cell proliferation was then analyzed by CCK8 assay, and cell migration was determinedby transwell assay. The gene expression and the phosphorylation of c-Met were examined by realtime-PCR and western blotting, respectively. Finally, the secretion of HGF was detected by ELISA assay. Results: c-Met, activated protein kinase (AMPK), and tyrosine kinase A (TRKA) were inhibited by SIM-89 with the IC50 values of 297 nmol/L, 1.31 μmol/L, and 150.2 nmol/L, respectively. SIM-89 exerted adenosine triphosphate (ATP) competitive inhibition on c-Met. Moreover, the expressions of STAT1, JAK1, and c-Met in H460 cells were decreased by SIM-89 treatment, and c-Met phosphorylationwas suppressed in A549, H441, H1299, and B16F10 cells by the treatment. In addition, SIM-89 treatment significantly decreased the level of HGF, which accounted for the activation of c-Met receptor tyrosine kinase. Finally, we showed cell proliferationinhibition and cell migration suppression in H460 and H1299 cells after SIM-89 treatment. Conclusion: In conclusion, SIM-89 inhibits tumor cell proliferation, migration and HGF autocrine, suggesting it’s potential antitumoractivity.