The Production and Cytological Analysis of Brassica napus-B Genome Chromosome Monosomic Addition Lines and Their Hybrids

Mao Teng Li,Jun Xiang,Jian Min Liu,Long Jiang Yu,Dian Rong Li 한국유전학회 2008 Genes & Genomics Vol.30 No.2

The Brassica napus-B genome monosomic addition lines (MALs) (AACC + B`, 2n = 39) were developed from self-pollination of pentaploid hybrids (AABCC) that were derived from hybridization between hexaploid hybrids (AABBCC) and B. napus (AACC). The alien chromosomes of the B genome in MALs were identified by the GISH technique, by observation of the meiotic behavior of pollen mother cells (PMCs), and by B-genome-specific molecular marker analysis. Studies of the meiotic behavior of B. napus-B genome chromosome MALs at diakinesis revealed that the majority of the chromosome configuration was 19II+1I, which indicated that the alien B genome chromosome remained univalent in most cases. The laggard-free PMCs also appeared at a lower ratio, which indicated that the B genome chromosome could be transmitted into gametes. The chromosome configurations of 20II and 19II+2I that appeared in double MALs (AACC+ 2 chromosomes of the B genome) indicated different homoeology between different B genome chromosomes. The paired B genome bivalent in double MALs can be normally segregated at anaphase in most cases. PMCs with multivalents were observed in all the double MAL combinations, which indicated homology of the B genome chromosomes with the A or C genome chromosomes.

Proteomics Analysis of Rice Proteins Up-regulated in Response to Bacterial Leaf Streak Disease

Dongxiao Li,Liangjiang Wang,Shaolei Teng,Guoguang Zhang,Lijia Guo,Qian Mao,Wei Wang,Min Li,Liang Chen 한국식물학회 2012 Journal of Plant Biology Vol.55 No.4

Bacterial leaf streak (BLS), caused by the pathogen Xanthomonas campestris pv. Oryzicola, is a major rice disease in tropical and subtropical regions of Asia. Rice proteins responsive to BLS are still not well characterized. We took a proteomics approach to identify the proteins that are up-regulated in rice leaves after infection. Approximately 1,500 protein spots were detected on each 2-D gel after silver-staining; those with increased protein levels were selected for MALDI-TOF-MS analysis. We identified 32 up-regulated proteins that might be involved in disease resistance signal transduction, pathogenesis, and regulation of cell metabolism. By using publicly available microarray data, we determined the mRNA transcripts of 23 proteins expressed in the leaves. Seven genes were analyzed by northern blots, which demonstrated that transcript levels were increased after bacterial infection. Our findings help elucidate the molecular mechanisms underlying BLS and provide a solid foundation for further research on the functions of relevant genes.

Modeling and simulating of center plate torsion behavior during bogie curve and turnout negotiation

Wang Shibo,Mao Yong,Teng Bing,Pan Li 대한기계학회 2014 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.28 No.7

In order to accurately simulating the tribological behavior of center plate of bogie, a model was built to calculate the torsional kinematicsparameters of the center plate when a railway freight-car bogie passes through a curve and a railway turnout. According to the torsionalkinematics parameters, a plate-on-plate torsional friction tester was built. The variation of the torsional angle, angular speed, andangular acceleration of the center plate under different conditions was investigated. The torsional angle was affected by the distancesbetween bogie centers and the radius of the railway circular curve. For different railway track geometries, the torsional angle of the centerplate during bogie curve and turnout negotiation ranged within 0.016°-1.08° and 0.04°-1.96°, respectively. The torsional speed of thecenter plate under the turnout condition was much higher than that under the curve condition. The torsional friction behavior of a centerplate material was simulated with the tester according to the calculated results.

Joint distribution of wind speed and direction in the context of field measurement

Hao Wang,Tianyou Tao,Teng Wu,Jianxiao Mao,Aiqun Li 한국풍공학회 2015 Wind and Structures, An International Journal (WAS Vol.20 No.5

The joint distribution of wind speed and wind direction at a bridge site is vital to the estimation of the basic wind speed, and hence to the wind-induced vibration analysis of long-span bridges. Instead of the conventional way relying on the weather stations, this study proposed an alternate approach to obtain the original records of wind speed and the corresponding directions based on field measurement supported by the Structural Health Monitoring System (SHMS). Specifically, SHMS of Sutong Cable-stayed Bridge (SCB) is utilized to study the basic wind speed with directional information. Four anemometers are installed in the SHMS of SCB: upstream and downstream of the main deck center, top of the north and south tower respectively. Using the recorded wind data from SHMS, the joint distribution of wind speed and direction is investigated based on statistical methods, and then the basic wind speeds in 10-year and 100-year recurrence intervals at these four key positions are calculated. Analytical results verify the reliability of the recorded wind data from SHMS, and indicate that the joint probability model for the extreme wind speed at SCB site fits well with the Weibull model. It is shown that the calculated basic wind speed is reduced by considering the influence of wind direction. Compared to the design basic wind speed in the Specification of China, basic wind speed considering the influence of direction or not is much smaller, indicating a high safety coefficient in the design of SCB. The results obtained in this study can provide not only references for further wind-resistance research of SCB, but also improve the understanding of the safety coefficient for wind-resistance design of other engineering structures in the similar area.

Disruption of the F-actin Cytoskeleton and Monolayer Barrier Integrity Induced by PAF and the Protective Effect of ITF on Intestinal Epithelium

Ling-fen Xu,Cheng Xu,Zhi-Qin Mao,Xu Teng,Li Ma,Mei Sun 대한약학회 2011 Archives of Pharmacal Research Vol.34 No.2

To explore whether platelet-activating factor (PAF) can disrupt the intestinal epithelial barrier directly and is associated with structural alterations of the F-actin-based cytoskeleton, and to observe the protective effect of intestinal trefoil factor (ITF), we establish an intestinal epithelia barrier model using Caco-2 cells in vitro. Transepithelial electrical resistance and unidirectional flux of lucifer yellow were measured to evaluate barrier permeability; immunofluorescent staining and flow cytometry were applied to observe morphological alterations and to quantify proteins of the F-actin cytoskeleton: the tight junction marker ZO-1 and Claudin-1 were observed using immunofluorescent staining. PAF significantly increased paracellular permeability, at the same time, F-actin and tight junction proteins were disrupted. It was thought that ITF could reverse the high permeability by restoring normal F-actin, ZO-1 and Claudin-1 structures. These results collectively demonstrated that PAF plays an important role in the regulation of mucosal permeability and the effects of PAF are correlated with structural alterations of the F-actin-based cytoskeleton and of tight junctions. ITF can protect intestinal epithelium against PAF-induced disruption by restricting the rearrangement of the F-actin cytoskeleton and of tight junctions.

Joint distribution of wind speed and direction in the context of field measurement

Wang, Hao,Tao, Tianyou,Wu, Teng,Mao, Jianxiao,Li, Aiqun Techno-Press 2015 Wind and Structures, An International Journal (WAS Vol.20 No.5

The joint distribution of wind speed and wind direction at a bridge site is vital to the estimation of the basic wind speed, and hence to the wind-induced vibration analysis of long-span bridges. Instead of the conventional way relying on the weather stations, this study proposed an alternate approach to obtain the original records of wind speed and the corresponding directions based on field measurement supported by the Structural Health Monitoring System (SHMS). Specifically, SHMS of Sutong Cable-stayed Bridge (SCB) is utilized to study the basic wind speed with directional information. Four anemometers are installed in the SHMS of SCB: upstream and downstream of the main deck center, top of the north and south tower respectively. Using the recorded wind data from SHMS, the joint distribution of wind speed and direction is investigated based on statistical methods, and then the basic wind speeds in 10-year and 100-year recurrence intervals at these four key positions are calculated. Analytical results verify the reliability of the recorded wind data from SHMS, and indicate that the joint probability model for the extreme wind speed at SCB site fits well with the Weibull model. It is shown that the calculated basic wind speed is reduced by considering the influence of wind direction. Compared to the design basic wind speed in the Specification of China, basic wind speed considering the influence of direction or not is much smaller, indicating a high safety coefficient in the design of SCB. The results obtained in this study can provide not only references for further wind-resistance research of SCB, but also improve the understanding of the safety coefficient for wind-resistance design of other engineering structures in the similar area.

Potential Antitumor Activity of SIM-89 in Non-Small Cell Lung Cancer Cells

Jun Pei,Baohui Han,Tianqing Chu,Minhua Shao,Jiajun Teng,Huifang Sha,Aiqing Gu,Rong Li,Jialin Qian,Weifeng Mao,Ying Li 연세대학교의과대학 2017 Yonsei medical journal Vol.58 No.3

Purpose: c-Met and its ligand, hepatocyte growth factor (HGF), play a critical role in oncogenesis and metastatic progression. The aim of this study was to identify inhibited enzymogram and to test the antitumor activity of SIM-89 (a c-Met receptor tyrosine kinaseinhibitor) in non-small cell lung cancer. Materials and Methods: Z’-LYTE kinase assay was employed to screen the kinase enzymogram, and mechanism of action (MOA) analysis was used to identify the inhibited kinases. Cell proliferation was then analyzed by CCK8 assay, and cell migration was determinedby transwell assay. The gene expression and the phosphorylation of c-Met were examined by realtime-PCR and western blotting, respectively. Finally, the secretion of HGF was detected by ELISA assay. Results: c-Met, activated protein kinase (AMPK), and tyrosine kinase A (TRKA) were inhibited by SIM-89 with the IC50 values of 297 nmol/L, 1.31 μmol/L, and 150.2 nmol/L, respectively. SIM-89 exerted adenosine triphosphate (ATP) competitive inhibition on c-Met. Moreover, the expressions of STAT1, JAK1, and c-Met in H460 cells were decreased by SIM-89 treatment, and c-Met phosphorylationwas suppressed in A549, H441, H1299, and B16F10 cells by the treatment. In addition, SIM-89 treatment significantly decreased the level of HGF, which accounted for the activation of c-Met receptor tyrosine kinase. Finally, we showed cell proliferationinhibition and cell migration suppression in H460 and H1299 cells after SIM-89 treatment. Conclusion: In conclusion, SIM-89 inhibits tumor cell proliferation, migration and HGF autocrine, suggesting it’s potential antitumoractivity.