LETM1 Promotes Gastric Cancer Cell Proliferation, Migration, and Invasion via the PI3K/Akt Signaling Pathway

Zhang, Yunfeng,Chen, Lele,Cao, Yifan,Chen, Si,Xu, Chao,Xing, Jun,Zhang, Kaiguang The Korean Gastric Cancer Association 2020 Journal of gastric cancer Vol.20 No.2

Purpose: Globally, there is a high incidence of gastric cancer (GC). Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is reported to play a vital role in several human malignancies. However, there is limited understanding of the role of LETM1 in GC. This study aims to investigate the effects of LETM1 on proliferation, migration, and invasion of GC cells. Materials and Methods: The expression levels of LETM1 in the normal gastric mucosal epithelial cells (GES-1) and GC cells were analyzed by quantitative real-time polymerase chain reaction and western blotting. CCK-8, wound healing, and Transwell invasion assays were performed to evaluate the effect of LETM1 knockdown or overexpression on the proliferation, migration, and invasion of the GC cells, respectively. Additionally, the effect of LETM1 knockdown or overexpression on GC cell apoptosis was determined by flow cytometry. Furthermore, the effect of LETM1 knockdown or overexpression on the expression levels of PI3K/Akt signaling pathway-related proteins was evaluated by western blotting. Results: The GC cells exhibited markedly higher mRNA and protein expression levels of LETM1 than the GES-1 cells. Additionally, the knockdown of LETM1 remarkably suppressed the GC cell proliferation, migration, and invasion, and promoted the apoptosis of GC cells, which were reversed upon LETM1 overexpression. Furthermore, the western blotting analysis indicated that LETM1 facilitates GC progression via the PI3K/Akt signaling pathway. Conclusions: LETM1 acts as an oncogenic gene to promote GC cell proliferation, migration, and invasion via the PI3K/Akt signaling pathway. Therefore, LETM1 may be a potential target for GC diagnosis and treatment.

Hybrid Phased-MIMO SAR : Mode Design and Performance Analysis

Lele Zhang,Dianren Chen 보안공학연구지원센터 2016 International Journal of Signal Processing, Image Vol.9 No.9

In this paper, we introduce an innovative scanning synthetic aperture radar (SAR) termed as the Hybrid Phased-MIMO SAR (HPMSAR) and investigate the system performance. The HPMSAR overcomes the limitations imposed by the conventional scanning SAR (ScanSAR, spotlight SAR, or Terrain Observation by Progressive Scans (TOPS) SAR) and presents the strong flexibility in imaging mode. Every subarray in HPMSAR is simultaneously pointed in the different directions and electronically steers the azimuth beam. This not only increases the illumination time per subswath entailing a high azimuth resolution but also covers an ultra-wide swath giving short-term time for global coverage. However, multidirectional scanning imaging will produce overlapped echoes and ambiguities on receive, then this paper presents the advanced processing scheme based on two-dimension (2-D) digital beamforming (DBF) with multichannel unambiguous reconstruction technology. The key of this method lies that multichannel processing technology in azimuth ensures receiving echoes free of aliasing by using a low PRF, and 2-D DBF could effectively suppress ambiguities and separate the overlapped echoes from different subswaths. Performance analysis compared with traditional methods shows output performance of interference suppression.

Discovering Station Patterns of Urban Transit Network with Multisource Data: Empirical Evidence in Jinan, China

Hui Zhang,Xu Li,Lele Zhang,Wei Wang,Jianmin Jia,Baiying Shi 대한토목학회 2021 KSCE Journal of Civil Engineering Vol.25 No.2

The various performances of buses at stations bring lots of difficulties for operators to manage them to improve the service quality. This paper proposes a data-driven framework to analyze the patterns of stations with network structure data, points of interest (POI) data and vehicle global positioning system (GPS) trajectory data. First, we build six indicators based on these data to measure the performance from station perspective. The results show that the number of POI around stations within 1 kilometer follows an exponential distribution. Moreover, the average headway and headway deviation of stations follow lognormal distributions. Second, we use agglomerative hierarchical clustering method to divided bus stations into different groups. Results indicate that the bus stations of Jinan could be divided into four groups with obvious characteristics. The findings could help operators to make exclusive strategies to manage bus systems.

Combined treatments of chitosan and sodium silicate to inhibit Alternaria alternata pathogens of postharvest winter jujube

Jingru Zhang,Xiaowen Cheng,Lulu Chang,Lele Zhang,Shaoying Zhang 한국식품과학회 2021 Food Science and Biotechnology Vol.30 No.4

Alternaria alternata is a pathogenic fungus thatinfects jujube fruit and leads to serious economic losses. Inthis paper, the antifungal activity of chitosan combinedwith sodium silicate against A. alternata in vitro andin vivo was investigated, and the possible antimicrobialmechanisms were explored. Results showed that the sporegermination and colony expansion of A. alternata weresignificantly inhibited by chitosan. Chitosan treatmentinduced the leakages of intercellular electrolytes, nucleicacids, and soluble protein of A. alternata. Meanwhile,chitosan damaged the cell morphology and membraneintegrity of A. alternata. The combination of chitosan andsodium silicate was more effective than chitosan alone. Inaddition, the effect of chitosan and sodium silicate couldsignificantly decrease natural rot rate and delay lesionexpansion of winter jujube. Collectively, chitosan combinedwith sodium silicate had the potential to controlpostharvest diseases of fruit caused by A. alternata.

LETM1 Promotes Gastric Cancer Cell Proliferation, Migration, and Invasion via the PI3K/Akt Signaling Pathway

Yunfeng Zhang,Lele Chen,Yifan Cao,Si Chen,Chao Xu,Jun Xing,Kaiguang Zhang 대한위암학회 2020 Journal of gastric cancer Vol.20 No.2

Purpose: Globally, there is a high incidence of gastric cancer (GC). Leucine zipper-EF-hand containing transmembrane protein 1 (LETM1) is reported to play a vital role in several human malignancies. However, there is limited understanding of the role of LETM1 in GC. This study aims to investigate the effects of LETM1 on proliferation, migration, and invasion of GC cells. Materials and Methods: The expression levels of LETM1 in the normal gastric mucosal epithelial cells (GES-1) and GC cells were analyzed by quantitative real-time polymerase chain reaction and western blotting. CCK-8, wound healing, and Transwell invasion assays were performed to evaluate the effect of LETM1 knockdown or overexpression on the proliferation, migration, and invasion of the GC cells, respectively. Additionally, the effect of LETM1 knockdown or overexpression on GC cell apoptosis was determined by flow cytometry. Furthermore, the effect of LETM1 knockdown or overexpression on the expression levels of PI3K/Akt signaling pathway-related proteins was evaluated by western blotting. Results: The GC cells exhibited markedly higher mRNA and protein expression levels of LETM1 than the GES-1 cells. Additionally, the knockdown of LETM1 remarkably suppressed the GC cell proliferation, migration, and invasion, and promoted the apoptosis of GC cells, which were reversed upon LETM1 overexpression. Furthermore, the western blotting analysis indicated that LETM1 facilitates GC progression via the PI3K/Akt signaling pathway. Conclusions: LETM1 acts as an oncogenic gene to promote GC cell proliferation, migration, and invasion via the PI3K/Akt signaling pathway. Therefore, LETM1 may be a potential target for GC diagnosis and treatment.

SNP Discovery from Transcriptome of Cashmere Goat Skin

Lele Wang,Yanjun Zhang,Meng Zhao,Ruijun Wang,Rui Su,Jinquan Li 아세아·태평양축산학회 2015 Animal Bioscience Vol.28 No.9

The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associated with genes affecting economic traits. Validation of single nucleotide variations in a whole-transcriptome sequencing is critical for understanding the information of molecular genetics. In this paper, we aim to develop a large amount of convinced single nucleotide polymorphisms (SNPs) for Cashmere goat through transcriptome sequencing. In this study, the transcriptomes of Cashmere goat skin at four stages were measured using RNA-sequencing and 90% to 92% unique-mapped-reads were obtained from total-mapped-reads. A total of 56,231 putative SNPs distributed among 10,057 genes were identified. The average minor allele frequency of total SNPs was 18%. GO and KEGG pathway analysis were conducted to analyze the genes containing SNPs. Our follow up biological validation revealed that 64% of SNPs were true SNPs. Our results show that RNA-sequencing is a fast and efficient method for identification of a large number of SNPs. This work provides significant genetic resources for further research on Cashmere goats, especially for the high density linkage map construction and genome-wide association studies.

Effect of anti-skin disorders of ginsenosides- A Systematic Review

Lele Cong,Jinli Ma,Yundong Zhang,Yifa Zhou,Xianling Cong,Miao Hao 고려인삼학회 2023 Journal of Ginseng Research Vol.47 No.5

Ginsenosides are bioactive components of Panax ginseng with many functions such as anti-aging, antioxidation,anti-inflammatory, anti-fatigue, and anti-tumor. Ginsenosides are categorized into dammarane,oleanene, and ocotillol type tricyclic triterpenoids based on the aglycon structure. Based on thesugar moiety linked to C-3, C-20, and C-6, C-20, dammarane type was divided into protopanaxadiol (PPD)and protopanaxatriol (PPT). The effects of ginsenosides on skin disorders are noteworthy. They play antiagingroles by enhancing immune function, resisting melanin formation, inhibiting oxidation, andelevating the concentration of collagen and hyaluronic acid. Thus, ginsenosides have previously beenwidely used to resist skin diseases and aging. This review details the role of ginsenosides in the anti-skinaging process from mechanisms and experimental research.

SNP Discovery from Transcriptome of Cashmere Goat Skin

Wang, Lele,Zhang, Yanjun,Zhao, Meng,Wang, Ruijun,Su, Rui,Li, Jinquan Asian Australasian Association of Animal Productio 2015 Animal Bioscience Vol.28 No.9

The goat Capra hircus is one of several economically important livestock in China. Advances in molecular genetics have led to the identification of several single nucleotide variation markers associated with genes affecting economic traits. Validation of single nucleotide variations in a whole-transcriptome sequencing is critical for understanding the information of molecular genetics. In this paper, we aim to develop a large amount of convinced single nucleotide polymorphisms (SNPs) for Cashmere goat through transcriptome sequencing. In this study, the transcriptomes of Cashmere goat skin at four stages were measured using RNA-sequencing and 90% to 92% unique-mapped-reads were obtained from total-mapped-reads. A total of 56,231 putative SNPs distributed among 10,057 genes were identified. The average minor allele frequency of total SNPs was 18%. GO and KEGG pathway analysis were conducted to analyze the genes containing SNPs. Our follow up biological validation revealed that 64% of SNPs were true SNPs. Our results show that RNA-sequencing is a fast and efficient method for identification of a large number of SNPs. This work provides significant genetic resources for further research on Cashmere goats, especially for the high density linkage map construction and genome-wide association studies.

Improved strategies for the load-bearing capacity of aluminum-PVC foam sandwich floors of a high-speed train

Haifeng Chang,Lele Zhang,Weiyuan Dou,Haifeng Zhang 대한기계학회 2021 JOURNAL OF MECHANICAL SCIENCE AND TECHNOLOGY Vol.35 No.2

For high-speed trains (HSTs), interface failures often occur in their aluminumPVC foam sandwich floor, the service life of which can affect the maintenance economy significantly. In this study, a floor model considering crushable foam layer and cohesive element was implemented to validate its bending behavior, with which the load-bearing capacity of actual floor structures under different loading conditions was analyzed. Accordingly, two strategies were presented for improving the load-bearing capacity of the floor: adding grid stiffened ribs on the aluminum layer and adjusting the layout of the supporting seats appropriately. The numerical simulation results indicated that the position of the maximum stress concentration was on the edge of the floor foam layer, and the limits of the load-bearing capacity were 50 and 575 kPa with respect to two operation conditions. By varying the supporting seats with different layouts or adding reinforced structures, the maximum stress and deflection in the critical zones were reduced significantly compared with those of the original design.

Design & Research of Legal Affairs Information Service Platform Based on UIMA and Semantics

Wang Chen,Zhang Jia,Qin Lele 보안공학연구지원센터 2016 International Journal of Future Generation Communi Vol.9 No.3

The law is the most powerful weapon to safeguard national stability and ensure flourishing of all causes as well as an instrument to protect the rights and interests of the masses, therefore, the just and accurate use of legal provisions is of crucial importance. With the increase of informatization level of the legal industry itself, more and more services in legal affairs are provided in information-based forms, and there is a large number of unstructured information in such business data. It’s a problem the legal industry needs to tackle in information management to rapidly acquire valuable content from the massive unstructured data and make use of such content. Based on analysis of problems arising in existing laws and regulations informatization system, this paper comes up with solution of a legal affairs information service platform based on cloud computing, UIMA, semantics, big data and Chinese word segmentation. This paper also proposes the four-layer technical framework solution on the basis of the design of integration and management method of unstructured data of heterology and isomerism, analyzing and processing method of unstructured data, semantics based unstructured information retrieval method and construction and maintenance method of ontology library. It also provides detailed introduction to the realization of the combination of big data and cloud computing and its application in this information platform by virtue of designing UFS - a distributed file system, MapReduce - a batch processing technology and BigTable - a distributed database. Data acquisition and expanded data analysis can be conducted by making use of the expandable UIMA framework and the sequential indexing of data content and analysis results can be materialized by means of applying Lucene indexing technology. With regard to information retrieval, the concept of ontology is introduced on the basis of traditional search model and a new search model based on domain ontology is proposed. IKAnalyzer 3.x is proposed to facilitate Chinese word segmentation. By taking advantage of such information service platform, legal affairs enterprises can effectively integrate structured and unstructured information resources and implement the storage, analysis, retrieval and decision-making applications of business data content.