Slide Session : OS-IFD-07 ; Infectious Disease : In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus

( Myung Jin Lee ),( Kye Hyung Kim ),( Jong Youn Yi ),( Su Jin Choi ),( Chung Jong Kim ),( Nak Hyun Kim ),( Kyoung Ho Song ),( Pyoeng Gyun Choi ),( Ji Hwan Bang ),( Wan Beom Park ),( Eu Suk Kim ),( San 대한내과학회 2014 대한내과학회 추계학술대회 Vol.2014 No.1

In Vitro Antiviral Activity of Ribavirin Against Severe Fever with Thrombocytopenia Syndrome Virus Myung Jin LEE1, Kye-Hyung KIM1, Jongyoun YI2, SuJin CHOI1, Chung-Jong KIM1, Nak- Hyun KIM1, Kyoung-Ho SONG1, Pyoeng Gyun CHOI1, Ji-Hwan BANG1, Wan Beom PARK1, Eu Suk KIM1, Sang-Won PARK1, Hong Bin KIM1, Nam Joong KIM1, Myoung- Don OH1 Seoul National University College of Medicine, Korea1, Pusan National University School of Medicine, Korea2 Background: Severe fever with thrombocytopenia syndrome (SFTS) is an emerging infectious disease caused by a novel Bunyavirus, severe fever with thrombocytopenia syndrome virus (SFTSV). No effective antiviral therapy is proven yet, but clinical use of ribavirin (RBV) has been tried. We investigated the antiviral effect of RBV against SFTSV in vitro. Methods: To test for cytotoxicity of RBV, Vero cells were treated with different concentrations of RBV (3.90 to 500 μg/mL, two-fold dilution) and analyzed by cell viability MTS assay 48h post-infection. To determine antiviral activity of RBV against SFTSV, Vero cells were infected with SFTSV strain Gangwon/Korea/2012 at 100 TCID50 (50% tissue culture infective dose) per well in a 96-well plate, and RBV was added at the concentrations showing no or minimal cytotoxicity. Viral RNAs were extracted from the culture supernatants and quantifi ed using one-step real-time reverse transcription- PCR to amplify the partial large segment of SFTSV. Statistical analysis was done by one-way ANOVA with Tukey`s post hoc test. Results: Cytotoxicity due to RBV was not observed at RBV concentration =31.3 μg/ mL. Viral RNAs at 24h post-RBV treatment were reduced with increasing RBV concentrations (1-32 μg/mL), compared with those of mock-treated cells (P <0.01, Figure). Half maximal inhibitory concentration (IC50) of RBV was 3.69 μg/mL at 24h post-RBV treatment. Conclusions: Our study shows that RBV has antiviral effect against SFTSV in a dose-dependent manner. Further studies are required to evaluate the effi cacy of RBV in SFTS.

성장속도에 따른 대장균 rnpB 유전자 발현의 조절작용

전은순,이학란,이석재,정영환,조명선,정재훈,이영훈,박충웅 全北大學校 基礎科學硏究所 1994 基礎科學 Vol.16 No.-

Growth rate-dependent expression of the rnpB gene encoding M1 RNA, the RNA component of Escherichia coli RNase P, was examined using a hybrid transdcuing λ phage. The hybrid transducing λ phage was constructed carrying the lacZ gene fused to the promoter for the rnpB gene. The amounts of β-galactosidase were analyzed in the E. coli lysogen carrying this transducing phage grown in different growth media to see dependence of the rnpB transcription on growth rate. The synthesis of β-galactosidase from the rnpB-lacZ fusion increased with growth rate. This result suggests that the rnpB promoter activity is dependent on the growth rate.

네모나프라이드의 유효성 및 추체외로 부작용 : Haloperidol 및 Risperidone과의 자연관찰적 개방형 비교연구 A Naturalistic Open Comparative Study with Haloperidol and Risperidone

서영수,김용관,신동환,공보금,이정구,박정환,윤성환,정치영,이상경,김영훈 대한생물치료정신의학회 2001 생물치료정신의학 Vol.7 No.2

Objectives : This open prospective study was performed in order to evaluate the efficacy and extrapyramidal adverse effects of nemonapride in the schizophrenic patients, and was compared wit one of typical antipshchotics. haloperidol and one of atypical antipsychotics, risperidone. Methods : Thirty male and female schizophrenic patients(DSM-Ⅳ) were treated for 12 weeks with haloperidol(n=10), risperidone(n=10) and nemonapride(n=10). The overall efficacy was assessed at baseline, 1st week, 2nd week, 4th week, 8th week, and 12th week by the Positive and Negative Syndrome Scale for Schizophrenia(PANSS). Also the overall safety was assessed in the same time period by the Extrapyramidal Symptom Rating Scale(ESRS). Results : There were no significant differences in PANSS scores(total, positive, negative, and general psycho­pathology subscale) among haloperidol, risperidone, and nemonapride groups, Treatment responders, defined as at least 20% reduction of baseline total PANSS score, were achieved by 8 patients(80%) in haloperidol group, 10 patients(100%) in risperidone group, and 8 patients(80%) in nemonapride group. And there were no significant differences in ESRS total scores among haloperidol, risperidone, and nemonapride group. Inter-group comparison among haloperidol, risperidone and nemonapride group, as assessed by the ESRS, revealed no significant differences in the shifts to the maximum score, 9.4(±9.4), 6.2(±8.4), and 11.3(±8.1) respectively, and also revealed no significant differences in the mean time reaching the maximum score, 4.5(±4.5) week, 7.8(±4.8) week, and 4.6(±4.4) week, respectivily. Conclusion : There wee no significant differences in the efficacy and extrapyramidal adverse effects among haloperidal, rispecridone and nemonapride groups. These results suggest that nemonapride was as efficacious and safe in he treatment of schizophrenia, as well known and widely used antipsychotics, haloperidol and risperidone.

아동행동 조사표에 의한 입원한 행동장애 청소년 분류

이환배,백기청,곽영숙 大韓神經精神醫學會 1992 신경정신의학 Vol.31 No.2

To make a suchclassification of conduct disorder by using CBCL. the authors investigated 37 conduct disorders adolescents in Seoul National Mental Hospital during the period from June 1, 1987 to June 30, 1991. The results were as follows : 1) The total numbers of conduct disorder adolescents are 37 patient, and 4 clusters are subclassified. 2) In one way ANOVA. the scores of externalizing and internalizing showed statistically significance. 3) To compare the score of subscale factor, in parent form CBCL, delinquent, aggressive, hyperactive factor showed statistically significance, and the remainder factors are not significant. 4) In comparison between clinical data and clusters, the age of symptom onset showed statistically significance, and orders are cluster 4, cluster 2, cluster 3 and cluster 1. The X2 test, the sex showed statistically significant the ratio of females 60.0% in cluster 2, 14.3% in cluster 3, 12.5% in cluster 1 and 0% in cluster 4. 5) i) In the main and interaction effects between 4 clusters and 2 forme of CBCL in each matched CBCL subscale, external, internal. aggressive(P<0.001) and deliniquent(P<0.01) factor showed statistically significant. ii) In the two forms of CBCL, social, school and total factor showed statistically significant. The remainder factor showed statistically no significance. iii) In the clusters and two forms of CBCL. the external and aggressive and internal factor showed statistically significance and in the cluster 1, the score of YSR of external and aggressive factor are higher than parent form CBCL, and in cluster 3 the scores of parent form and higher. In cluster 4, the score of internal factor of parent form CBCL is higher than the self form.

유도전동기의 자속제어형 Inverter에 의한 가변속 운전

이승환,우정인,이준탁 동아대학교 공과대학 부설 한국자원개발연구소 1987 硏究報告 Vol.11 No.2

Many techniques for variable speed drives of induction motor had been introduced. Recently, all of them are replaced by PWM control techniques with transitor inverter. But the troublesome problems appeared in variable speed drives include the determination of PWM switching patterns, the generation of control signals, the minimization of torque pulsation, acoustical noises, mechnical vibrations, harmonics and etc.. Therefore, the pursuits of high performance drives and compactness in control circuits without the aids of micro-computer or micro-processor with the functions of control and simple calculations are impossible. In this paper, one technique for the digital and software processing of all control factors is introduced. The determinations of PWM switching patterns are based on the selection of flux vector equivalent to the integral value of applied voltage during every sampling period. And, the real flux vector is controlled so that the trajectory of it can be tracked to the circularform one of ideal flux vector. Also, introduction of the concept of g-u-w plane from d-p plane make digitization and quantization of all control factors convenient. Specially, the predicted control techniques in selection of differential flux vector, Δλ, have functions which can compensate the delay time due to the micro-processor processing, so the real time control system by 8086 micro-processor, simulation and experimental results are in detail compared each other.

미소원공결함을 갖는 자동차 전륜구동 축재의 피로수명 예측

이동길,오환교,정재강 朝鮮大學校 機械技術硏究所 1998 機械技術硏究 Vol.1 No.1

본 연구에서는 현재 생산되고 있는 자동차의 전륜 구동축재료 S43C와 S50C를 대상으로 회전굽힘피로시험을 통해 고주파열처리와 온도조건에 따른 피로크랙 전파거동을 고찰하고, Nisitani가 제안한 역학적 변수에 의해 재료상수를 구한 후 그 적용성을 검토해 보았다. 비열처리재 S43C 및 S50C의 피로한도는 각각 98.1MPa, 107.9MPa이었으며, 열처리재는 각각 147.1MPa, 156.9MPa로서 열처리 후 두 재료 모두 피로한도가 약 49MPa씩 증가하였으며, 저온(-30℃)에서의 피로수명은 상온(25℃)에서보다 두 재료 모두 비열처리재는 약 110%, 열처리재는 약 120% 증가하였다. 또한 본 재료에 실험으로부터 얻어진 재료상수를 Nisitani의 피로크랙전파속도 예측식에 적용하여 피로수명을 예측한 결과 고주파 열처리 여부와 실험온도 조건에 관계없이 약 5% 내외의 낮은 오차를 보이며 잘 일치함을 알았다. In this study, the rotary bending fatigue test was carried out with two kinds of materials, S43C and S50C, using in the front engine and front drive shaft of vehicle. The specimens were heat-treated by high frequency induction method and tested at +25℃ and -30℃ in order to look over the influence of the heat treatment and the temperatures. The mechanical factor, C(2a)p, proposed by Nisitani was employed in the present study to compare the results of fatigue life obtained from experiment and prediction. The fatigue limit of the as-received specimens S43C and S50C were 98.1MPa and 107.7MPa, and that of the heat-treated S43C and S50C were shown 147.1MPa and 156.9MPa respectably. It means that the fatigue limit was increased about 49MPa by high frequency heat treatment. Fatigue lifes of the specimens tested at low temperature(-30℃) showed about 110% for as-received, 120% for heat-treated higher than at room temperature(25℃). It was found the crack length at onset or final fracture tested at low temperature was shorter than the crack length at room temperature(+25℃). The fatigue lifes were predicted with an equation of the fatigue crack propagation proposed by Nisitani, d(da/dN)=C(2a)p, the results were found to be within 5.0% error by comparison with experimented results.

Baclofen 投與에 따른 精神分裂症 患者의 血淸 成長호르몬의 反應

李圭煥,白仁浩,高孝進,李正杓,咸雄,李奎恒 서울醫大 1990 精神醫學 Vol.15 No.1

Ultra-energy-efficient analog-to-digital converters based on single-electron transistor/CMOS hybrid technology for biomedical applications

Lee, Jieun,Choi, Changmin,Park, Sungwook,Chung, In-Young,Kim, Chang-Joon,Park, Byung-Gook,Kim, Dong Myong,Kim, Dae Hwan Institute of Physics 2009 Semiconductor science and technology Vol.24 No.11

<P>Ultra-energy-efficient analog-to-digital converters (ADCs) based on single-electron transistor (SET)/complementary metal-oxide-semiconductor (CMOS) hybrid technology are proposed as a solution to sense and process biomedical signals. Our results show the energy efficiency of 0.82 pJ/state, which is lower than that in previously reported energy-efficient ADCs. The performance and dissipated power of proposed ADCs are estimated and compared with those of CMOS ADCs by using Lee's SPICE model including non-ideal effects of the experimental data. While the proposed ADC shows an operating power lower by two orders of magnitude than that of the CMOS flash-type ADC, the number of required transistors is about 10% of that in the CMOS flash-type ADC. The peak-to-valley current ratio in Coulomb oscillation of SETs used in the circuit implementation has the range of 1.15–1.5, which is consistent with the experimental result of top-down approached Si-based SETs at <I>T</I> = 77–100 K. From the perspective of the immunity to the gate capacitance <I>C<SUB>cg</SUB></I> mismatch and the background charge <I>Q</I><SUB>0</SUB> noise, it is shown that the criteria of SET/CMOS hybrid ADCs are Δ<I>C<SUB>cg</SUB></I> ⩽ 0.02 × <I>C<SUB>cg</SUB></I> (with <I>C<SUB>cg</SUB></I> = 0.24 aF) and Δ<I>Q</I><SUB>0</SUB> ⩽ 0.23<I>q</I>, respectively.</P>

PLL Motor Control System의 安定度와 動特性 改善에 관한 硏究

李承煥,禹靖仁,安仁模 동아대학교 공과대학 부설 한국자원개발연구소 1983 硏究報告 Vol.7 No.1

Method of stabilizing a PLL controlled DC motor drive using velocity feedback and acceleration feedback are described. By a choice of the time constants in the feedback circuits astable system with good performance characteristics can be obtained. Integral squared error(ISE) criterian is used to find out the constants of the stabilizing circuits for giving an optimum response. Besides, EMF feedback loop added in the system make the system respond rapidly for a sudden variation of load torque. Transient response of the system is calculated for a sudden loading condition and then it is compared with experimental results.

균등 변환을 이용한 고속 프랙탈 영상 부호화

정환익,이광형 崇實大學校 生産技術硏究所 2002 論文集 Vol.32 No.-

Image compression based on fractal theory is known to have high compression rate and fast decoding time. However, it is limitedly used in practice since it spends too long time to find matching blocks in encoding process. In other to reduce the encoding time we propose a new method to reduce the number of comparisons using the isometry transformation. The proposed method classify the blocks into one of four types such as plain, middle, vertical/horizontal, or diagonal block. The block search time can be reduced by applying the 8 isometry transformation process in different ways depending on the block types. The experiments show that the proposed method reduces the compression times up to minimum 35% smaller than those of 8 isometry transformations while maintaining PSNR of 27∼30dB.