미세유화약물송달시스템을 이용한 로바스타틴의 생체이용률 향상

윤복영,강복기,정상영,이영원,이시범,황성주,육순홍,강길선,이해방,조선행 한국약제학회 2002 Journal of Pharmaceutical Investigation Vol.32 No.4

A self-microemulsifying drug delivery system (SMEDDS) was developed to increase the dissolution rate, solubility, and ultimately bioavailability of a poorly water soluble drug, lovastatin. SMEDDS was the mixtures of oils, surfactants, and cosurfactants, which emulsify under conditions of gentle agitation, similar to those which would be encountered in the gastro-intestinal (GI) tract. Various types of self-emulsifying formulations were prepared using four types of oil (Capryol 90, Lauroglycol 90, Labrafil M 1944 CS and Labrafil M 2125), two surfactants (Cremophor EL and Tween 80), and three cosurfactants (Carbitol, PEG 400 and propylene glycol). The efficiency of emulsification was studied using a laser diffraction size analyzer to determine particle size distributions of the resultant emulsions. Optimized formulations selected for bioavailability assessment were Carpryol 90 (40%), Cremophor EL (30%) and Carbitol (30%). SMEDDS containing lovastatin (20 mg and 5 mg) were compared to a conventional lovastatin tablet (Mevacor^? , 20 mg/tab) by the oral administration as prefilled hard gelatin capsules to fasted beagle dogs for in vivo study. The area under the serum concentration-time curve from time zero to the last measured time in serum, AUC_0→24h, was significantly greater in SMEDDS, suggesting that bioavailability increase 130% and 192% by the SMEDDS, respectively. The self-emulsifying formulations of lovastatin afforded the improvement in absolute oral bioavailability relative to previous data of lovastatin tablet formulation. These data indicate the utility of dispersed self-emulsifying formulations for the oral delivery of lovastatin and potentially other poorly absorbed drugs.

서울의 Penicillinase Producing Neisseria gonorrhoeae 발생빈도(1998)

김재홍,김준호,반재용,이정우,황성주,정준규,정성태,강진문,조흔정,홍창의,정혜신,이한승,김이선,이봉길,이종호,선영우,한기덕,윤성필,이성훈,안종성,박석범,문승현,조항래,김형섭,류지호,황재영,박준홍,손상욱 한양대학교 의과대학 2001 한양의대 학술지 Vol.21 No.1

In recent years, gonorrhea has been pandemic and remains one of the most common STDs in the world, especially in developing countries. For the detection of a more effective therapeutic regimen and assessing the prevalence of Penicillinase Producing Neisseria gonorrhoeae(PPNG), we have been trying to study the patients who have visited the Venereal Disease Clinic of Choong-Ku Public Health Center in Seoul since 1980 by menas of the chromogenic cephalosporin method. In 1998, 93 strians of N. genorrhoeae were isolated, among which 60(64.5%) were PPNG. The prevalence of PPNG in Seoul, which had been decreased to 39% in 1996 after a peak of 74.3% in 1993, is increased to 64.5% in 1998.

Canine Distemper Virus 검출을 위한 정량 Real-time PCR

이승관,이창규,정운원,조경진,김현숙,강영대,서장훈 高麗大學校 倂設 保健大學 保健科學硏究所 2002 保健科學論集 Vol.28 No.2

Canine distemper virus(CDV) infection causes a fatal systemic disease in wide range of carnivore species, including domestic dogs. It has been a highly contagious disease of veterinary importance for centurise. Recently many researches show that qualitative RT-PCR for the detection of CDV was sensitive and specific method for the early and safe antemortem diagnosis of distemper by using whole blood regardless of clinical sign and pathological finding. Recently, results were correlated to clinical findings, anti-CDV neutralizing antibody titers, postmortem findings, and demonstration of CDV NP(nucleoprotein) antigen by immunohistochemistry. We have studied a quantitative real-time PCR assay for CDV in order to improve and broaden the clinical usefulness of RT-PCR assay. Total 37 samples including 9 clinically positive and 29 randomly selected ones have investigated. CDV RNA was extracted from whole blood. The isolated RNA was reverse-transcripted into cDNA followed by PCR amplification. The assay based on real-time PCR using SYBR Green I dye as quantitative method with a LightCycler. Calibration curves covered 30-3×10^(8) copies of CDV were created using cloned 240bp CDV cDNA of viral RNA. CDV was detected all in 8 positive samples whereas 8 in 29 randomly collected ones. The detection ranges of clinical CDV specimens were various from 3×10² to 3×10⁴ copies. Comparision of real-time PCR to conventional PCR showed equal sensitivity and specificity. But real-time PCR method has a merit of quantification as well as more rapid results than those of common RT-PCR. CDV can be accurately detected and quantified in the whole blood from the dog infected with CDV. This quantitative technique may improve the clinical utility of RT-PCR for the detection of CDV.

初步者를 위한 Tennis Service의 段階的인 指導方法에 關한 硏究

이영흥,홍성표 忠南大學校體育科學硏究所 1984 體育科學硏究誌 Vol.2 No.1

This research was tried to find out the effective way to teach tennis service for the beginners. The object of study was composed of 8 students of the first grade in girl's high school who have not taken special lessons for tennis but wanted to learn it, was divided them into two groups. Experimental and comparative group(each has 4 students). They have taken lesson for 2 hours a day, four times in a week through 24 weeks. The investigator divided the teaching program of slice service and top spin service into three steps, taught them he same procedure in the first and second step. In the last step the experimental group has learned ball toss, spin, ball control but the other group has learned only service. After comparing and analysising the outcome of the experimental group's development, the research reached the following conclusion. 1. In raising tole success rate of service, the experimental group which practices ball toss, spin and ball control step by step records higher meaningful level than the comparative one which repeats only service after nine weeks. 2. As the better way to coach slice service and top spin service to the beginner, we can conclude to Practice ball toss, spin and ball control step by step is more effective than simple and repeated practice at the ideal position after learning form.

고령화 사회와 여가 레크레이션의 발전 방안

이계영,김흥태 대진대학교 1999 大眞論叢 Vol.7 No.-

The purpose of this study is firstly to try to find a development plan of leisure recreation in the aging society, and ultimately to make a contribution to a development plan of leisure recreation suited for the future society in the 21st century. The main contents consist of three aspects, and they also become conclusinos of this study. In the policy aspect of the leisure recreation of aging society, We must play great part in improvement of ability which can sensitively cope with a change of age and a turn to a form of leisure. In the actual aspect, the development plans as follows; First, expansion and supplementation of the facilities for old people's leisure recreation. Second, expansion and maximization of utilization for the public gymnasium facilities. Third, efficient reconsideration of facility management for old peoples, leisure recreation. In the program aspect, the development plans as follows; First, diversification and specialization of programs for old people's education and reinforcement of substantial old peoples education. Second, development or spread of old peoples sports programs and activation of old peoples sports activities through and industrial upbringing with chart concerned with the old.

방사선 조사가 배양된 조골세포의 apoptosis와 세포주기의 변화 및 석회화 결절 형성에 미치는 영향에 관한 연구

이영미,최항문,허민석,이삼선,최순철,박태원 대한구강악안면방사선학회 2000 Imaging Science in Dentistry Vol.30 No.3

Purpose : The study was aimed to detect the induction of apoptosis, cell cycle arrest and calcified nodule formation after irradiation on primarily cultured osteoblasts. Materials and Methods : Using rat calvarial osteoblasts, the effects of irradiation on apoptosis, cell cycle arrest, and calcified nodule formation were studied. The single irradiation of 10 and 20 Gy was done with 5.38 Gy/min dose rate using the 137Cs cell irradiator at 4th and 14th day of culture. Apoptosis induction and cell cycle arrest were assayed by the flowcytomtry at 1,2,3, and 4 days after irradiation. The formation of calcified nodules was observed by alizarin red staining at 1,3,10,14 days after irradiation at 4th day of culture, and at 1,4,5 days after irradiation at 14th day of culture. Results : Apoptosis was not induced by 10 or 20 Gy independent of irradiation and culture period. Irradiation did not induced Gl arrest in post-irradiated ostedblasts. After irradiation at 4th-day of culture, G2 arrest was induced but it was not statistically significant after irradiation at 14th-day of culture. In the case of irradiated cells at 4th day of culture, calcified nodules were not formed and at 14th-day of culture after irradiation, calcified nodule formation did not affected. Conclusion : Taken together, these results suggest that Irradiation at the dose of 10-20 Gy would not affect apoptosis induction of osteoblasts. Cell cycle and calcified nodule formation were influenced by the level of differentiation of osteblasts. (Korean J Oral Maxillofac Radiol 2000; 30: 189-198)

소상공업의 생산성향상을 위한 소상공인지원센터 개선방안

이의영,김항석 한국생산성학회 2004 生産性論集 Vol.18 No.3

World economy makes rapid progress due to knowledge based economy, globalization, regionalization, and netwPork oriented system. Rapid progress in IT industry and networking. The importance of small business is highlighted as one of the confrontations for these variations. Therefore, the purpose of this survey is groping for the development plan which make the revitalization of the small business support and the higher productivity possible. This survey consists of four chapters. Chapter one is the introduction and deals with the background, the purpose, the method of this study. Chapter two examines the transition of its position, the enlargement of the role, and the present state of the small business. Chapter three is the study about the actual conditions of the support center for small business and the revitalization plan. And Chapter four is the conclusion and presents the results, the limits, and the hereafter assignment of this study.

미국의 스포츠 매니지먼트 연구동향 분석

이강우,육조영,문행자,백광 한국스포츠리서치 2004 한국 스포츠 리서치 Vol.15 No.2

The graduates of sports management are employed about to amateur organization, company sports department, country club, resort, public institution, citizen sports center, horse racing, pro-sports team, management or marketing of university games, athlete agency and the campus games and clubs like to recreation sports. Sports management gives the possibility enough to people that interested in sports career. The future of the field depends on the talent' success that finished to sports management process.

公衆保健醫 및 保健診療員의 業務時間 活用에 關한 調査 硏究

李恒榮,沈雲澤 충남대학교 의과대학 지역사회의학연구소 1985 충남의대잡지 Vol.12 No.1

For the purpose of offering fundamental data to the development of medical delivery system, the author analysed the daily activities and time allocations of Public Health Doctors occupied Health Subcenters and Community Health Practitioners in Chungnam area, March 1 through the April 30, 1984. Data was collected from 26 Public Health Doctors and 7 Community Health Practitioners. The obtained results were as follows; 1. The average daily working hours was 6.7(range 6.9-7.6), but that was 4.2 on Saturday. 2. The average daily working hours by working place difference were own clinic 6.0, home visiting 0.5 and others clinic 0.2. Of the total 6.7 daily working hours, 3.2 was treatment activity, 2.9 was administrative activity and 0.6 was health service activity. 3. The classification of diseases being treated were disease of respiratory system, digestive system, skin & cellular tissue, muscle & skeletal system, accidentㆍ poisoning & violence, infectious & parasitic disease in that order. 4. Average number of daily health service activity were tuberculosis control 4.8, child health 4.2, health education & environmental sanitation 2.8, maternal health 2.5, but those of administrative activity were admintrative affair 10.3 and support work 9.0.

중등학교 교사의 역할 수행에 대한 교사-학생의 지각에 관한 연구

이항재,오영순 順天鄕大學校 人文科學硏究所 1998 순천향 인문과학논총 Vol.6 No.-