Review of three black fungus gnat species (Diptera: Sciaridae) from greenhouses in China Three greenhouse sciarids from China

Lan Ye,Ruixin Leng,Junhao Huang,Cheng Qu,HongWu 한국응용곤충학회 2017 Journal of Asia-Pacific Entomology Vol.20 No.1

Bradysia odoriphaga Yang et Zhang, 1985 syn. nov. is proposed as new synonym of Bradysia cellarum Frey 1948, and Lycoriella pleuroti Yang et Zhang, 1987 syn. nov. as new synonym of Lycoriella ingenua (Dufour, 1839). These results were confirmed by cytochrome oxidase I (COI) DNA barcodes, neighbor-joining (NJ) trees, and a revision of their characters. Illustrations of the morphological characteristics of these two species are provided. Former published records of Bradysia difformis Frey 1948 fromChina accord with Bradysia impatiens (Johannsen, 1912), as also shown by COI NJ tree in this paper.

Electrochemical Behavior of Sm(III) on the Aluminium-Gallium Alloy Electrode in LiCl-KCl Eutectic

Ye, Chang-Mei,Jiang, Shi-Lin,Liu, Ya-Lan,Xu, Kai,Yang, Shao-Hua,Chang, Ke-Ke,Ren, Hao,Chai, Zhi-Fang,Shi, Wei-Qun Korean Radioactive Waste Society 2021 방사성폐기물학회지 Vol.19 No.2

In this study, the electrochemical behavior of Sm on the binary liquid Al-Ga cathode in the LiCl-KCl molten salt system is investigated. First, the co-reduction process of Sm(III)-Al(III), Sm(III)-Ga(III), and Sm(III)-Ga(III)-Al(III) on the W electrode (inert) were studied using cyclic voltammetry (CV), square-wave voltammetry (SWV) and open circuit potential (OCP) methods, respectively. It was identified that Sm(III) can be co-reduced with Al(III) or Ga(III) to form Al_zSm_y or Ga_xSm_y intermetallic compounds. Subsequently, the under-potential deposition of Sm(III) at the Al, Ga, and Al-Ga active cathode was performed to confirm the formation of Sm-based intermetallic compounds. The X-ray diffraction (XRD) and scanning electron microscopy-energy dispersive spectroscopy (SEM-EDS) analyses indicated that Ga₃Sm and Ga₆Sm intermetallic compounds were formed on the Mo grid electrode (inert) during the potentiostatic electrolysis in LiCl-KCl-SmCl₃-AlCl₃-GaCl₃ melt, while only Ga₆Sm intermetallic compound was generated on the Al-Ga alloy electrode during the galvanostatic electrolysis in LiCl-KCl-SmCl₃ melt. The electrolysis results revealed that the interaction between Sm and Ga was predominant in the Al-Ga alloy electrode, with Al only acting as an additive to lower the melting point.

Explore the dynamic alternation of gene PLAC4 mRNA expression levels in maternal plasma in second trimester for nonivasive detection of trisomy 21

( Lan Yang ),( Hai Yan Sun ),( Dao Zhen Chen ),( Mu Dan Lu ),( Ye Tang ),( Jian Pin Xiao ) 대한산부인과학회 2015 Obstetrics & Gynecology Science Vol.58 No.4

Objective: Noninvasive prenatal detection of trisomy 21 (T21) has been achieved by measuring the ratio of two alleles of a single nucleotide polymorphism in circulating placenta specific 4 (PLAC4) mRNA in maternal plasma with a few assays in recent years. Our research is to explore the variations of PLAC4 mRNA expression level in maternal plasma with normal pregnancies in second trimester, which can provide pregnant women deeper insights with suitable detection period for the non-invasive prenatal detection of T21. Methods: We measured a serial plasma PLAC4 mRNA concentrations weekly from the same 25 singleton normal pregnant women. We recruited maternal plasma samples from 45 singleton pregnant women , comprising of 25 euploid pregnancies (control group; range, 17 to 21 weeks) and 20 T21 pregnancies (T21 group; range, 19 to 24 weeks). With the application of reverse transcription polymerase chain reaction, we achieved an insight of PLAC4 mRNA expression levels in maternal plasma during second trimester with euploid pregnancies. Results: Among the control group, the levels of PLAC4 mRNA expression in the gestation of 17 to 18 weeks were significantly less than those in the gestation of 18 to 21 weeks (P<0.05). The average PLAC4 mRNA concentration of the normal pregnant women was not higher than that of the T21 group (P>0.05). Conclusion: The PLAC4 mRNA showed a higher level of expression in the gestation of 18 to 21 weeks with an euploid pregnancy of pregnant women. We also found that there was no significant difference in plasma PLAC4 mRNA concentration between the normal and the T21 pregnancies in second trimester.

Two type III polyketide synthases from Polygonum cuspidatum: gene structure, evolutionary route and metabolites

Lan-Qing Ma,Yan-Wu Guo,Hui-Li Guo,Xing Li,Li-Li Huang,Bo-Ning Zhang,Xiao-Bin Pang,Ben-Ye Liu,Hong Wang 한국식물생명공학회 2013 Plant biotechnology reports Vol.7 No.3

In our recent work (Ma et al., in Planta229(3):457–469, 2009a and 229(4):1077–1086, 2009b),two three-intron type III PKS genes, PcPKS1 and PcPKS2,were isolated from Polygonum cuspidatum Sieb. et Zucc. Phylogenetic and functional analyses revealed PcPKS1 is athree-intron chalcone synthase (CHS) gene, and PcPKS2 isfound to be a three-intron benzalacetone synthase (BAS)gene. The regular CHS encoded by a single intron genehave not been isolated and characterized from P. cuspidatum. In this work a further CHS with one intron (PcPKS3)and a stilbene synthase (STS) gene with three-intron(PcPKS5) were isolated and characterized by functionaland phylogenetic analyses. In comparison with PcPKS1, abifunctional enzyme with both CHS and BAS activity, theenzymatic product of recombinant PcPKS3 was naringenin,bis-noryangonin (BNY) and 4-coumaroyltriacetic acidlactone (CTAL) occurred as side products. The PcPKS5synthesized resveratrol and a trace amount of naringeninfrom p-coumaroyl-CoA. To our knowledge, PcPKS5 is thefirst reported three-intron STS gene in flowering plants. Inthis work, we speculated that this involved a possibleevolutionary route of plant-specific type III PKS superfamilyin P. cuspidatum.

Utilization of Single Nodal Cuttings and Therapies for Eradicating Double-Infected Potato Virus (PLRV, PVY) from In Vitro Plantlets of Potato (Solanum tuberosum)

Yi Lan Fang,Shambbu Prasad Dhital,Kui Hua Li,Dong Man Khu,Hae Young Kim,Ye Su Song,Hak Tae Lim 한국원예학회 2005 Horticulture, Environment, and Biotechnology Vol.46 No.2

In vitro plantlets of potato genotype ‘F9-99’ infected with PLRV and PVY were used for the virus eradication using heat and chemical treatments. Subculture medium was added with acetylsalicylic acid (ASA) (10??, 10??, and 10?⁴ M) and ribavirin (10, 15, and 20 ㎎ㆍL?¹) alone or in combinations. The plantlets were incubated at 25℃ (control), and alternating heat treatments 35/30℃ for 1, 4, and 8 h or 42/25℃ for 4 h. Virus status of the plantlets were examined by means of double antibody sandwich-enzyme linked immunosorbent assay and reverse transcription polymerase chain reaction. After 4?8 weeks of treatment, 40, 51, 41, 36, and 0% survival were observed in the control (MS and 25℃), ASA plus ribavirin (25℃), MS plus heat treatment (35/30℃), ASA plus ribavirin (35/30℃), and MS medium with heat treatment (42/25℃), respectively. As for virus eradication, heat treatment (35/30℃) with 1 h alternating thermo-cycle resulted in 100% PVY elimination, but it was much more difficult to remove the PLRV from the double-infected potato plantlets with PVY and PLRV. The highest rate of PLRV eradication was 60% from the combined treatment of 10?? M ASA, 20 ㎎ㆍL?¹ ribavirin, and heat treatment at 35/30℃ for 8 h alternating temperature.

Shen-Kang protects against tacrolimus-induced renal injury

Long Ye Zhang,Jian Jin,Kang Luo,Shang Guo Piao,Hai Lan Zheng,Ji Zhe Jin,임선우,최범순,양철우,Can Li 대한내과학회 2019 The Korean Journal of Internal Medicine Vol.34 No.5

Background/Aims: Evidence suggests that Shen-Kang (SK), a traditional Chinese herbal medicine, protects against various types of renal injury. In this study, we evaluated whether SK treatment confers renoprotection in a rat model of chronic tacrolimus (TAC) nephropathy. Methods: Rats were treated daily with TAC (1.5 mg/kg, subcutaneously) and SK (450 mg/kg, intravenously) for 4 weeks. The effects of SK on TAC-induced renal injury were assessed by measuring renal function, urine albumin excretion, histopathology, inflammatory cell infiltration, expression of profibrotic (transforming growth factor β1 [TGF-β1] and TGF-β inducible gene-h3 [βig-h3]) and proinflammatory cytokines, oxidative stress, and apoptotic cell death. Results: Administration of SK preserved glomerular integrity (fractional mesangial area and Wilms tumor 1-positive glomeruli), attenuated tubulointerstitial fibrosis, and reduced the number of ectodermal dysplasia 1-positive cells, and this was paralleled by improved urine albumin excretion and renal dysfunction. At the molecular level, SK treatment suppressed expression of TGF-β1/Smad2/3, βig-h3, and proinflammatory cytokines. Oxidative stress and apoptotic cell death were significantly decreased with SK treatment, and apoptosis-related genes were regulated toward cell survival (active caspase-3 and the B-cell lymphoma-2/ Bcl2-associated X [Bcl-2/Bax] ratio). Conclusions: SK protects against TAC-induced renal injury.

Transonic characteristics for AGARD Wing 445.6 by numerical simulation

Wenjuan Ye,Youngshin Lee,Jinhai Lan 한국추진공학회 2010 한국추진공학회 학술대회논문집 Vol.2010 No.5

The supersonic speeds slowing down by shock waves is a common problem during the transonic region. So how to study the status of shock on the surface of airplane and wings is crucial adjective during transonic region. However, the theoretical and computational transonic flow problems were very hard. This paper introduced using Navier-Stokes Schemes to study characteristics of AGARD Wing 445.6 by ANSYS CFX in transonic region. From simulations results, as the Mach number increases, shock waves appear in the flowfield, getting stronger as the speed increases, these shock waves will lead to a rapid increase in drag.

CD44v3 and VEGF-C Expression and its Relationship with Lymph Node Metastasis in Squamous Cell Carcinomas of the Uterine Cervix

Liu, Ye-Qing,Li, Hai-Feng,Han, Jing-Jing,Tang, Qiong-Lan,Sun, Qing,Huang, Zhi-Quan,Li, Hai-Gang Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.12

Background: To investigate the expression of CD44v3 and vascular endothelial growth factor-C (VEGF-C) and their relationship with lymph node metastasis in squamous cell carcinomas (SCC) of the uterine cervix. Materials and Methods: Expression of CD44v3 and VEGF-C was analyzed in 109 cases of cervical SCC by immunohistochemistry (IHC). The relationship was analyzed between expression and the patient age, histological differentiation, formation of tumor emboli in lymphoid vessels, lymph node metastasis, FIGO staging, and TNM classification. Results: Expression rates for both CD44v3 and VEGF-C were 43.1% in cervical SCC. The cells with positive immunohistochemical staining of CD44v3 were distributed mainly around the keratin pearls in well differentiated carcinomas, but distributed diffusely in the moderately and poorly differentiated lesions. VEGF-C was found stained positively in most of the tumor cells. There were differences in expression between normal epithelium and atypical hyperplasia as well as carcinoma. Both CD44v3 and VEGF-C were found to be associated positively with lymph node metastasis and TNM classification (both p=0.000). Neither CD44v3 nor VEGF-C was found to be associated with patient age, histological differentiation, formation of tumor emboli in lymphoid vessels and FIGO staging. CD44v3 was found to be associated with VEGF-C positively (p=0.000). Conclusions: Abnormal expression of CD44v3 and VEGF-C is associated closely with the lymph node metastasis in cervical SCC, and these agents may cooperate in carcinogenesis and development of metastatic lesions.

Integrated Map-matching Algorithm Based on Fuzzy Logic and Dead Reckoning

Yan-Lan Yang,Hua Ye,Shu-Min Fei 제어로봇시스템학회 2010 제어로봇시스템학회 국제학술대회 논문집 Vol.2010 No.10

Based on the concept of fuzzy logic, a new map-matching algorithm was proposed to reduce the GPS positioning error by means of position correction in use of the digital map. Firstly, three fuzzy rules are introduced in different aspects which may influent the credibility judgment of the best matching road. And the corresponding membership functions are defined in details separately for sub-evaluations of the direction consistency, the proximity and the track similarity against the original GPS position and its candidate road, where dead reckoning technique is referred. Secondly, the comprehensive evaluation performing as the unique basis for final judgment is made by doing simple addition of the results of these sub-evaluations with certain weights according to their contributions towards the credibility judgment. Finally, the original GPS position is revised by making a vertical projection onto the best matching road. Experimental results demonstrate the effectiveness and applicability of this algorithm in improving the GPS positioning accuracy.

Comparison of Physiological Characteristics for Storage Evaluation of Onion Lines and Commercial Varieties

Hyang Lan Eum,JiWon Han,Ye-Rin Lee,Pue Hee Park,Me-Hea Park,Jae-Han Cho 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.10