소 결합 환경에서 순환 질의 처리를 위한 형판 연산기법

황규영(Kyu-Young Whang),홍기형(Ki-Hyung Hong),이윤준(Yoon-Joon Lee) 한국정보과학회 1991 정보과학회논문지 Vol.18 No.3

본 논문에서는 소 결합 환경하에서 순환 질의의 한 부류를 효율적으로 처리하기 위한 template 처리 기법을 제안하고, 그 정당성을 증명하였다. Template 처리 기법은 주 기억장치내의 릴레이션과 이차 기억장치에 저장되어 있는 릴레이션 사이의 비용이 비싼 결합 연산(join)을 완전히 제거함으로써 순환 질의를 효과적으로 처리한다. 본 기법으로 처리할 수 있는 질의의 부류는 Whang과 Navathe가 제안한 확장된 논리합 정규형(extended disjunctive normal form)으로 표현하였을 때, 하나의 비환형 트리(nonlooped-tree)와 하나의 단일환형 트리(single looped tree)로 표현된다. We present the template evaluation technique for efficient processing of a class of recursive logic queries in a loosely-coupled environment. Using this technique, queries are evaluated efficiently by avoiding costly joins between an in-memory relation residing in main memory and a database relation residing in the secondary storage. The queries in this class are those represented by one nonlooped tree and one single-looped tree (indicating linear recursion) in the extended disjunction normal form proposed by Whang and Navathe. We also prove the correctness of the template evaluation technique.

리보플라빈을 가진 수용성 백금(Ⅱ)착체의 합성과 항암활성 및 세포주기와의 관계

황규자,권영이 숙명여자대학교 약학연구소 1999 약학논문집-숙명여자대학교 Vol.16 No.-

A series of vitamine-containing Pt(II) complexes of the type [Pt(Rf)(L)], (where Rf=riboflavin, L=ethylenediamine, 1,3-propanediamine, 1,4-butanediamine) was synthesized and characterized by IR, electronic absorption, elemental analysis and FAB-Mass. The coordination sites of Rf to Pt(II) ions were determined to be N(5) and 0(6) with resultant chelate ring formation. These compouds have much better water solubility(145- 150mg/ml) than cisplatin has(lmg/ml). The anticancer activity of this Riboflavin-containing Pt(II) series was investigated by MTT assay against mouse and human leukemia cell lines in vitro. Interaction between Pt(II) complex and DNA were analyzed by flowcytometry, and showed G2/M arrest in LI210 cell line.

7원환 구조를 갖는 새로운 백금착체의 합성에 대한 연구

황규자,권영이 숙명여자대학교 약학연구소 1998 약학논문집-숙명여자대학교 Vol.14 No.-

Platinum complexes having 7 .membered ring structure with Riboflavin(RF) and Flavin mononucleotide(FMN) as leaving group were synthesized to enhance antitumor activity and solubility. Platinum complexes with RF and FMN were determined by ir spectra, nmr spectra, mass and elemental analysis.

변단면 H-형강보의 탄성처짐에 관한 연구

황상진,최선규,홍영균,홍기섭 대한건축학회 2003 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.23 No.2(구조계)

Tapered H-shaped beams are often used in the structures with large spans and roof crane running beams; for the purpose of member self-weight reduction and appropriately architectural representation. The purpose of this study is to represent an accurate calculation method for tapered beam deformations, based on elastic theories. In this study, equations for beam deformation calculation of whole span tapered or partly tapered beams are represented and compared with structural analysis results of generally used commercial computer program. The equations of Moment of Inertia for tapered H-shaped beams, which may have different width and thickness of their flanges or top and bottom flanges, are induced as well. Compared with the result of generally used structural analysis program, the results of this study will be confirmed for practical use.

새로운 백금착체의 설계와 그 항암활성

황규자,이근임,권영이 숙명여자대학교 약학연구소 1996 약학논문집-숙명여자대학교 Vol.12 No.-

Pt(Ⅱ) and Pt(Ⅳ) complexes having six-membered chelate rings with N,N'-bis(2-chloroethyl)propanediamine (2-CePn) as carrier ligand have been prepared and characterized by IR and ^1H NMR. These complexes were examined for their in vitro cytotoxicity against leukemia L1210 and P388 cell lines. The cytotoxicity of platinum complexes with 1,3-propanediamine were improved by introduction of 2-chloroethyl group which nitrogen mustard, and alkylating agent, contains. The Pt(Ⅱ) complex showed more effective cytotoxicity than Pt(Ⅳ) complex.

Peutz-Jeghers 증후군 1예

최규식,황영남,김영자,양윤모,최영호 대한가정의학회 1985 Korean Journal of Family Medicine Vol.6 No.12

전신성 홍반성 낭창의 임상적 고찰

신영태,김종학,장윤경,양종오,구영선,강민규,황평주,나기량,이강욱,서광선 충남대학교 의과대학 지역사회의학연구소 1998 충남의대잡지 Vol.25 No.1

The epidemiology, diagnostic criteria, clinical features, symptoms and signs laboratory findings, kidney pathology, and clinicopathologic correlation of systemic lupus erythematosus(SLE) were analyzed. The 63 patients studied were managed at the Department of Internal medicine, CNUH, from January 1983 to December 1997. Kidney biopsy was performed in 53 patients out of 63 patients with SLE. The results were as follows: 1. The ratio of male to female was 1:26. They were 12 to 71 years old and mean age was 32.1 years old. The peak age incidence was 4th decades(30%). 2. The most frequent chief complaint on admission was generalized edema. Most patients complain two or more symptoms. 3. Immunologic and renal disorders were the most frequently observed in the ARA criteria of SLE. And the positive ANA, hematologic disorder, malar rash, and arthritis, were observed in order of frequency. 4. Among the 53 patients with renal biopsy, 30 patients revealed class Ⅳ lupus nephritis(56%), class II in 12 patients(23%), class V in 8 patients(15%) and class III in 2 patients(6%). 5. The cases of lupus nephritis represented as nephrotic syndrome were high in the class IV with 68% and class V with 86%. 6. Of 30 patients who can be followed up, 5 patients resulted in death(17 % of mortality). They "were 14 to 57 years old and mean age was 28 years old. Follow up duration were from 1 month to 6 years and 3 months, and mean duration was 1 year and 1 month, but 3 cases 7. The causes of death were as follows: Two cases were due to seizure and coma resulted from CNS involvement. One was sepsis due to miliary the & pneumonia. One was dilated cardiomyopathy and heart failure due to cardiac ac involvement. And the other one was sepsis and acute renal failure resulted from cellulitis. 8. Prednisolone was used in all patients basically, and methyl-prednisolone pulse therapy, antimalarials and alkylating agents were used in some cases. In the cases of combined therapy, the activity of SLE was well controlled.

백반증 환자의 혈청이 배양된 정상 인체 멜라닌 세포의 세포고사에 미치는 영향에 관한 연구

김민영,이성열,박영립,이종석,황규왕 순천향의학연구소;Soonchunhyang Medical Research Institute 2000 Journal of Soonchunhyang Medical Science Vol.6 No.1

About the etiologies of vitiligo, so many hypotheses have been proposed, but the exact pathologic mechanism was not clear. Among those hypotheses, autoimmune theory is most popular and clinically it is often accompanied by many other autoimmune disorders such as thyroid disease, pernicious anemia, diabetes mellitus, Addison's disease, autoimmune hypoparathyroidism and organ specific antibodies. Investigator thought that apoptosis is important mechanism on the destruction of melanocyte by autoantibodies. To prove this, 10 autoimmune disorder-free, active and progressive vitiligo patient's sera were added to melanocytes culture media and incubated for 48 hours. After that, investigator observed the changes of melanocyte with TUNEL stain and Hoechst stain, which were well-known methods for apoptotic cell stain. The melanocytes incubated with vitiligo patients sera showed more apoptosis than that with normal person's sera. These results indicates that vitiligo patients' sera have some substances inducing apoptosis of melanocytes.

성인에서 Haemophilus influenzae 감염증의 임상양상과 미생물학적 특성

이종섭,황병연,정희진,김우주,박승철,이도현,이창규,신종희,황규잠,이영희 대한화학요법학회 2000 대한화학요법학회지 Vol.18 No.2

목적 : H. influenzae는 성인에서 흔히 호흡기 감염을 일으키는 균주로, 최근 ampicillin 내성 β-lactamase 생성 균주들이 지속적인 증가를 보이고 있어, 이에 국내 성인에서 H. influenzae에 의한 감염의 임상양상과 분리된 균주들의 미생물학적 특성을 조사하고자 하였다. 방법 : 1998년 3월부터 1999년 9월까지 고려대 구로병원에 내원한 환자중에서 H. influenzae가 분리된 68명을 대상으로 임상양상을 조사하고 항균제 감수성 검사를 시행하였다. 분리된 33주에 대해서 혈청형, 생물형, 세포외막 단백분석을 시행하였고, β-lactamase 생성여부에 따른 감염 환자군간 임상상 및 예후의 차이를 비교하였다. 결과 : 환자의 평균연령은 68세였고, 지역사회 획득감염이 전체의 85%를 차지하였다. 폐렴과 기관지염과 같은 하기도 감염이 전체환자의 75%를 차지하였고 분리 검체 역시 객담이 84%로 가장 많았다. 환자의 71%가 기저질환을 가지고 있었으며, 그 중에 만성 폐쇄성 폐질환, 심혈관 질환, 악성종양, 당뇨병이 대부분이었다. 혈청형으로는 nontypeable 형이 68%로 가장 많았고 b형은 한 예도 없었다. β-lactamase 생성율은 63.2%였고, β-lactamase 양성균주의 항균제 내성율이 음성균주에 비하여 높았으나 β-lactamase 생성 여부에 관계없이 cefotaxime, azithromycin, ciprofloxacin에 대하여 100%의 감수성을 나타내었고 cefaclor, cefuroxime에 대해서는 10% 이하의 낮은 내성율을 보여 이들 항균제들이 치료에 효과적일 것으로 사료된다. 세포외막 단백분석에서는 C형과 D형이 전체의 64%로 가장 많았으며, 혈청형과의 유의한 연관성은 보이지 않았다. 결론 : 성인에서 H. influenzae는 주로 만성 기저질환자에서 급성 호흡기감염 또는 만성 폐쇄성 폐질환의 급성 악화를 유발한다. 기존의 보고에 비해 β-lactamase 양성율이 증가하였고, 이에 따른 항균제 내성율도 증가하여 항균제의 선택에 유의하여야 할 것으로 생각된다. 세포외막 단백분석상 특정한 유형의 균주에 의해 감염이 집중적으로 발생하고 있어 향후 역학 조사의 도구로 이용할 수 있겠다. Purpose : H. influenzae is one of the common causative microorganisms of respiratory tract infections in adults. Recently. β-lactamase producing, ampicillin-resistant H. infiuenzae has become worldwide problem as well as in Korea. We studied to investigate the clinical features and microbiologic characteristics of H. infiuenzae infections in adults. Materials & Methods : From March 1998 to September 1999, 68 patients were enrolled in this study. Clinical features of H. infiuenzae infections were investigated, 68 isolates were subjected to the β-lactamase test, and 33 isolates were used for serotyping. biotyping, antibiotic susceptibility and outer membrane protein (OMP) analysis. Results : Mean age of study patients was 68 years-old and the acquisition rate in community was 85%. Pneumonia and bronchitis were the most common type of infection such as 80%, sputum was the most common clinical specimen for H. mQuenzae isolation. 70% of patients had chronic underlying diseases. Non-typeable strains were 68% and β-lactamase producing rate was 63%. There was no difference in the clinical features and prognosis of H. influenzae infection between the β-lactamase positivie and -negative groups. The susceptibility of cefotaxime, azithromycin, and ciprofloxacin was 100% and that of cefaclor, cefuroxime was under 10% in spite of β-lactamase positivity. In OMP analysis, C and D types were dominant (64%). Conclusion : H. influenzae caused acute respiratory infections in adults with chronic underlying disorders. This study shows higher β-lactamase producing rate and antibiotic-resistance rate than that were reported previously. OMP analysis shows that two major types of strains cause clinical infections intensively.

인체 흑색종 세포군에서의 Vascular Endothelial Growth Factor(VEGF) 분비

김현조,황규왕,조문균,박영립,이종성,이성열 순천향의학연구소 2001 Journal of Soonchunhyang Medical Science Vol.7 No.2

Backgroung : Vascular endothelial growth factor(VEGF) is an angiogenic cytokine expressed by many human and animal tumors. Ultraviolet radiation(UVR) has been causally linked to melanoma initiation and progression. UVR is a known melanocyte mutagen and induces specific mutations in cellular oncogenes such as p53 and ras. Activated H-ras activates tumor angiogenesis in other cell types, and angiogenic switching is accompanied by up-regulation of VEGF. Objective : The purpose of this study was to determine whether VEGF release from human melanoma cell lines was altered by activating ras mutations or by inactivation of p53 induction and whether VEGF release of these transfected melanoma cell lines was changed after UVR radiation. Results : 1. Release of VEGF from menolayer culture of melanoma cells was significantly higher in cells with activating ras mutations and lower in cells with expression of dominant negative p53. 2. In spheroid culture, dominant negative p53 decreases VEGF release in WM35 cells, but activating ras did not affect VEGF release significantly. 3. The control cell line(WM35Neo) had higher VEGF release in the spheroid culture than in the monolayer culture, and dominant negative p53 or activating ras did not affect VEGF release significantly between these two culture systems. 4. Higher cell density increases VEGF release in all tested melanoma cell lines was shown in both spheroid and monolayer culture. This cell density effect was strongest in cells with activated ras in monolayer culture. 5. UVR inhibits VEGF release in all tested melanoma cell lines, and this effect was stronger in monolayer culture than in spheroid culture. The effect of UVR on VEGF release was significantly lower in the DNp53 transfectants. 6. UVR decreased the cell viability in a dose dependent manner regardless of the cell density or cell culture system. Conclusion : VEGF release in human melanoma was inhibited by ultraviolet radiation at cytotoxic doses, and this effect is stronger in monolayer culture than in spheroid culture. VEGF release from melanoma cell lines was also influenced by p53, ras and cell growth conditions in a complex manner.