증류수와 인공타액에서 충전용 글래스 아이오노머 시멘트의 불소유리량에 대한 평가

김경남,이진숙,류지헌 大韓齒科器材學會 1994 대한치과재료학회지 Vol.21 No.2

The purpose of this study was to compare the amount and pattern of fluoride release from restorative cements into de-ionized water with that in artificial saliva . Three light-curing glass ionomers , a water-setting glass ionomer⁴, two conventional glass ionomers and a resin-based material were selected. Sixteen cylindrical samples(6.5mm×2.5mm)of each cement were prepared. Each was individually suspended in 5ml of either de-ionized water or artificial saliva(eight samples in each medium) and stored at 37℃. The media were changed and fluoride concentration was measured with fluoride electrode for 90 days. The data showed that: (1) Cumulative fluoride release from Ketac-Fil, Dyract and VariGlass VLC into the artificial saliva was significantly less than in de-ionized water(p<0.05) as tested by ANOVA. The cumulative amounts of fluoride into the saliva were 2.52, 0.10 and 0.65 ㎎F /㎤. And they accounts for 49%, 14% and 84% of the amounts into de-ionized water respectively; (2) Ketac-Fil released more fluoride than other materials in both media(p<0.001). Vitremer, Iono Gem, and Dyract released 37%, 38%, and 13% of the cumulative amount of fluoride from Ketac-Fil into deionized water, respectively, but they released 73%, 78%, and 4% of that into artificial saliva, respectively. It is concluded that the release of fluoride from some restorative glass ionomers is highly variable according to the medium in which glass ionomer is stored, and so artificial saliva should be used to test fluoride release of restorative glass ionomer cements.

Apolipoprotein E 다형성에 따른 사업장 근로자의 혈중 지질농도, 영양소 섭취 및 건강관련 생활습관

박유경,조상운,강지연,백윤미,성숙희,최태인 대한지역사회영양학회 2008 대한지역사회영양학회지 Vol.13 No.5

The purpose of this study was to investigate the association among nutrient intakes and health-related lifestyles with cardiovascular disease risk assessed by blood lipid profile according to Apolipoprotein E genotypes. Middle-aged industrial male workers who had completed their annual medical examination were recruited and data of 675 subjects who finished the nutrient survey were used in the analysis. Anthropometric parameters, dietary assessment (FFQ), health-related lifestyles and blood profiles were used for statistical analyses. Apo E genotype groups were classified into the following three genotypes: Apo E2 group (including E2/E2, E2/E3, E2/E4), Apo E3 group (including E3/E3), Apo E4 group (including E3/E4, E4/E4). The frequency of Apo E2, E3, and E4 allele were 13.3%, 75.0% and 11.7% respectively. There were no significant differences in the anthropometric parameters depending on different Apo E genotypes. Also, no significant differences in the nutrient intakes were found according to the genotype groups. The nutrient intakes of all subjects were similar to or higher than the level of KDRIs (Dietary Reference Intakes For Koreans) except for intakes of calcium (67.44% of KDRIs), vitamin A (73.83% of KDRIs) and vitamin B2 (78.02% of KDRIs). Also, there were no significant differences of health-related lifestyles according to Apo E genotype groups. As for the lipid profiles, Apo E4 group had significantly higher total and LDL-cholesterol concentrations than the Apo E2 group (p < 0.05). We confirmed that plasma total and LDL-cholesterol concentrations were greatly influenced by Apo E genotypes. However, nutrient intakes and health-related lifestyles were not associated with Apo E genotypes.

복수과 진료 환자의 중복 처방에 대한 분석 및 평가

이지은,장혜경,오지영,유윤경,김현지,임숙인,연숙희,강진숙,최귀령 한국병원약사회 2003 병원약사회지 Vol.20 No.1

In an aging society, there is an increasing possibility of the duplication of the drugs given to patients because they take many kinds of drugs. Among the prescriptions given to the patients who was treated at multiple ambulatory clinics in St. Mary's hospital for one month of September, 2002, in which drugs that has identical or similar effects are prescribed, we analysed and evaluated them by patients' characteristics, kinds of medication, severity of side effects on a case by case basis. More, we assessed the potential additional costs. As a result of this study, the duplication rate turned out to be 6.69% and it was shown that the proportion of the elderly patients over 60 was high. The gastro-intestinal medications took up a large part and most of the cases showed that the danger caused by double taking of medicine was slight, but there were some examples in which the serious side effects were predicted. Expected additional costs were the average 11.349 won and it ranges from the minimum of 56 won to the maximum of 135,720 won. In conclusion, the management of drug histories of the patients who need the plural treatments is very important and the necessity of the individual and professional guidance of taking medicines for the elderly patients is emerged, too.

Free Radical Scavenging Activity of Methanol Extracts of Chungkukjang

( Kyoung Chun Seo ),( Jeong Sook Noh ),( Na Ri Yi ),( Ji Myung Choi ),( Eun Ju Cho ),( Ji Sook Han ),( Yeong Ok Song ) 부산대학교 김치연구소 2009 김치의 과학과 기술 Vol.12 No.-

To further the goal of isolating Bacillus sp. from commercial chungkukjang (CKJ) for a development of a probiotic dietary adjunct using soymilk or milk, antioxidant activity of CKJ purchased from the Sunchang Traditional Village in Chunbook province WE examined. Six CKJ simples were evaluated and 3 were selected based on the results of the physicochemical analysis and sensory evaluation for further antioxidant study. IC50 for DPPH scavenging activity or methanol extracts of CKJ ranged from 238.1 to 345.7 μg/mL. CKJ exhibited over 80% scavenging of ·OH and ONOO- at concentrations of 100 μg/mL and 250 μg/mL, respectively. O2- and NO scavenging activities of three CKJ were increased in a dose dependent manner with the concentration tested from 100 to 1000 μg/mL. In this study, the methanol extract of CKJ exhibited a great reduction capability and powerful free radical scavenging activity, especially against OH- and ONOO-, which are the most toxic radicals responsible for oxidative damage in the body. However, radical scavenging effects of CKJ on DPPH, O2-, and nitrite radical were rather moderate. In conclusion, CKJ may reduce the oxidative stress in the body by scavenging the free radicals.

Bioactive Compounds / Food Microbiology : Apoptotic Effects of Cordycepin Through the Extrinsic Pathway and p38 MAPK Activation in Human Glioblastoma U87MG Cells

( Ji Sue Baik ),( Seo Won Mun ),( Kyoung Sook Kim ),( Shin Ji Park ),( Hyun Kyoung Yoon ),( Dong Hyun Kim ),( Min Kyu Park ),( Cheorl Ho Kim ),( Young Choon Lee ) 한국미생물 · 생명공학회 2016 Journal of microbiology and biotechnology Vol.26 No.2

We first demonstrated that cordycepin inhibited cell growth and triggered apoptosis in U87MG cells with wild-type p53, but not in T98G cells with mutant-type p53. Western blot data revealed that the levels of procaspase-8, -3, and Bcl-2 were downregulated in cordycepintreated U87MG cells, whereas the levels of Fas, FasL, Bak, cleaved caspase-3, -8, and cleaved PARP were upregulated, indicating that cordycepin induces apoptosis by activating the death receptor-mediated pathway in U87MG cells. Cordycepin-induced apoptosis could be suppressed by only SB203580, a p38 MAPK-specific inhibitor. These results suggest that cordycepin triggered apoptosis in U87MG cells through p38 MAPK activation and inhibition of the Akt survival pathway.

Free Radical Scavenging Activity of Methanol Extracts of Chungkukjang

Kyoung-Chun Seo,Jeong-Sook Noh,Nari Yi,Ji-Myung Choi,Eun-Ju Cho,Ji-Sook Han,Yeong-Ok Song 한국식품영양과학회 2008 Preventive Nutrition and Food Science Vol.13 No.2

To further the goal of isolating Bacillus sp. from commercial chungkukjang (CKJ) for a development of a probiotic dietary adjunct using soymilk or milk, antioxidant activity of CKJ purchased from the Sunchang Traditional Village in Chunbook province was examined. Six CKJ samples were evaluated and 3 were selected based on the results of the physicochemical analysis and sensory evaluation for further antioxidant study. IC50 for DPPH scavenging activity of methanol extracts of CKJ ranged from 238.1 to 345.7 ㎍/mL. CKJ exhibited over 80% scavenging of ?OH and ONOO- at concentrations of 100 ㎍/mL and 250 ㎍/mL, respectively. O₂? and NO scavenging activities of three CKJ were increased in a dose dependent manner with the concentration tested from 100 to 1000 ㎍/mL. In this study, the methanol extract of CKJ exhibited a great reduction capability and powerful free radical scavenging activity, especially against OH? and ONOO-, which are the most toxic radicals responsible for oxidative damage in the body. However, radical scavenging effects of CKJ on DPPH, O₂?, and nitrite radical were rather moderate. In conclusion, CKJ may reduce the oxidative stress in the body by scavenging the free radicals.

Free Radical Scavenging Activity of Methanol Extracts of Chungkukjang

Seo, Kyoung-Chun,Noh, Jeong-Sook,Yi, Na-Ri,Choi, Ji-Myung,Cho, Eun-Ju,Han, Ji-Sook,Song, Yeong-Ok The Korean Society of Food Science and Nutrition 2008 Preventive Nutrition and Food Science Vol.13 No.2

To further the goal of isolating Bacillus sp. from commercial chungkukjang (CKJ) for a development of a probiotic dietary adjunct using soymilk or milk, antioxidant activity of CKJ purchased from the Sunchang Traditional Village in Chunbook province was examined. Six CKJ samples were evaluated and 3 were selected based on the results of the physicochemical analysis and sensory evaluation for further antioxidant study. $IC_{50}$ for DPPH scavenging activity of methanol extracts of CKJ ranged from 238.1 to 345.7 ${\mu}g/mL$. CKJ exhibited over 80% scavenging of ${\cdot}OH$ and ONOO- at concentrations of 100 ${\mu}g/mL$ and 250 ${\mu}g/mL$, respectively. $O_2^-$ and NO scavenging activities of three CKJ were increased in a dose dependent manner with the concentration tested from 100 to 1000 ${\mu}g/mL$. In this study, the methanol extract of CKJ exhibited a great reduction capability and powerful free radical scavenging activity, especially against OH. and ONOO-, which are the most toxic radicals responsible for oxidative damage in the body. However, radical scavenging effects of CKJ on DPPH, $O_2^-$, and nitrite radical were rather moderate. In conclusion, CKJ may reduce the oxidative stress in the body by scavenging the free radicals.

Midazolam Inhibits Tumor Necrosis Factor-α–induced Endothelial Activation: Involvement of the Peripheral Benzodiazepine Receptor

Joo, Hee Kyoung,Oh, Sae Cheol,Cho, Eun Jung,Park, Kyoung Sook,Lee, Ji Young,Lee, Eun Ji,Lee, Sang Ki,Kim, Hyo Shin,Park, Jin Bong,Jeon, Byeong Hwa American Society of Anesthesiologists, Inc. 2009 Anesthesiology Vol.110 No.1

BACKGROUND:: Midazolam is widely used as an intravenous sedative. However, the role of midazolam on vascular endothelial activation is still unknown. The present study explores the action of midazolam on endothelial activation and its role to peripheral benzodiazepine receptor (PBR) in cultured human umbilical vein endothelial cells. METHODS:: Intracellular localization of PBR in human umbilical vein endothelial cells was visualized with immunofluorescent staining. Monocyte adhesion and vascular cell adhesion molecule-1 expression were measured with monocyte adhesion assay and Western blot analysis. Involvement of PBR was assessed by using specific antagonists and small interfering RNA against PBR. RESULTS:: PBR was localized in the mitochondria of human umbilical vein endothelial cells. Midazolam significantly inhibited tumor necrosis factor-α–induced vascular cell adhesion molecule-1 and monocyte adhesion in a dose-dependent manner (1–30 μM). The midazolam-mediated suppression on the tumor necrosis factor-α–induced vascular cell adhesion molecule-1 expression and monocyte adhesion were inhibited by the pretreatment of PK11195 and not inhibited by the flumazenil. Transfection of small interfering RNA for PBR decreased the expression of PBR (18 kDa) in human umbilical vein endothelial cells. Midazolam-mediated suppression on the tumor necrosis factor-α–induced vascular cell adhesion molecule-1 expression was abrogated by the transfection of small interfering RNA for PBR. CONCLUSION:: These results suggest that midazolam has an inhibitory action on the endothelial activation and that its action is related to the activation of peripheral benzodiazepine receptor localized in mitochondria of the endothelial cells.

제Ⅶ응고인자 다형성과 뇌경색

김지명,양혜경,김종성,박찬정,지현숙 대한임상병리학회 2001 대한임상병리학회지 Vol.21 No.4

배경: 응고인자 VI I 은 허혈성 심질환의 새로운 위험인자로 언급되면서 혈전 형성과정에 있어서의 역할이 관심의 대상이 되고 있다. 응고인자 VI I 은 환경적 요인과 유전적 요인에 의해 혈중 농도가 결정되는데 최근에는 응고인자 VI I 과 유전자 다형성의 연관성이 보고되고 있다. 그러나 허혈성 심질환과 많은 위험인자를 공유하는 뇌혈관 질환에서는 응고인자 VI I 과의 관련성을 조사한 것이 거의 없다. 본 저자들은 연령에 의한 영향을 배제하기 위해 50세 미만의 뇌경색 환자군과 정상 대조군을 대상으로 응고인자 VI I 과 유전자 다형성의 분포를 조사하고 뇌경색 발생과의 관련성도 알아 보고자 하였다.<br> 방법: 1999년 3월부터 12월까지 본원 신경과로 내원한 78명의 뇌경색 환자와 연령과 성별을 고려한 70명의 정상인을 대상으로 고혈압과 흡연력의 유무를 조사하였고 환자군은 혈관조영술상 혈전의 유무와 재원기간을 추가로 조사하였다. 이들에서 EDTA와 sodium citrate 혈액을 채취한 후 EDTA 혈장은 중성지방의 농도 측에, sodium citrate 혈장은 응고인자 VI I 의 농도 측정에 이용하였다. 혈구세포는 페놀-클로로폼을 이용한 추출법으로 DNA를 얻은 후 중합효소연쇄반응과 제한효소 Msp I 처리과정을 통해 R353Q 다형성을, 중합효소연쇄반응을 통해 hypervariable region 4 (HVR4) 다형성의 유전자형 빈도를 조사하였다.<br> 결과: 환자군과 대조군에서 응고인자 VI I 과 중성지방의 농도는 차이를 나타내지 않았으며 혈전의 유무나 재원기간에 따른 응고인자 VII의 농도 차이도 없었다. R353Q 다형성 유전자형 및 HVR4 다형성 유전자형의 빈도는 양 군에서 차이가 없었다. 그러나 두 유전자 다형성은 유전자형에 따라 의미있는 응고인자 VI I 의 농도 차이를 나타내어 환자군과 대조군 모두에서 M1M1이나 H6 H6 유전자형을 가진 경우 응고인자 VI I 의 농도가 높았다. 또한 응고인자 VI I 의 농도는 중성지방과 양의 상관관계를 나타냈는데 특히 M1M1 유전자형을 가진 여성에서 상관성이 보다 강했다.<br> 결론: 응고인자 VI I 의 농도는 유전자 다형성에 의해 조절되며 특히 M1M1 유전자형을 가지는 여성에서 중성지방과 강한 상관성을 가지는 것으로 관찰되었다. 그러나 본 연구에서는 응고인자 VII 농도나 유전자 다형성이 환자군과 대조군에서 차이를 나타내지 않아 뇌혈관 질환의 발생 및 예후와는 연관되지 않는 것으로 판단되었다. 그러나 응고인자 VII 농도나 유전자 다형성이 뇌혈관 질환의 발생에 관여하는 위험인자인가 여부를 밝히기 위해서는 전향적인 연구가 필요하리라 사료된다. Background: Factor VII:C(FVII:C) has been shown to be a risk factor of ischemic heart disease (IHD) and plasma levels are reported to be associated with polymorphisms of the FVII gene. Cerebrovascular disease (DVD) shares many of the risk factors associated with IHD but few studies about the relationship between FVII and CVD have been investigated. In this study, we sought to determine the relationship between FVII gene polymorphisms and cerebral infarct in the population below 50 years old.<br> Methods: The subjects were 78 patients with cerebral infarct who had been admitted between March and December 1999 and 70 controls, matched with age and sex. FVII R353Q and hypervariable region 4 (HVR4) polymorphisms were analyzed with allele specific PCR and restriction enzyme treatment. FVII:C assay was performed on the STAGO Compact analyzer. Total cholesterol and triglycerides were also measured.<br> Results: There was no significant difference in FVII:C, total cholesterol and triglycerides between patients and controls. The distribution of the FVII R353Q genotype and the HVR4 genotype also showed no differences in patients, compared to controls. But both polymorphisms were significantly associated with FVII:C levels in the patients and controls.<br> Conclusion: The level of FVII:C was related to FVII gene polymorphisms but there is no significant difference of FVII gene polymorphisms in the cerebral infarct population, compared to controls. Our study supports that neither FVII:C levels nor FVII genotypes are independently involved in the pathogenesis of cerebral infarct. In conclusion, the FVII genotype is a major predictor of plasma FVII:C levels but may not play an important role in the development of cerebral infarct.

Poster Session 2 : Oral Presentation ; Neurobiology : Characterization of a ternary complex with neurotrophin receptors and an ankyrin-rich membrane spanning protein

( Ji Hee Yu ),( Mi Kyoung Son ),( Jung Sun Cho ),( Han Jeong Park ),( Seung Pyo Lee ),( Ki Suk Paik ),( Moses V. Chao ),( Mi Sook Chang ) 한국생화학분자생물학회 (구 한국생화학회) 2005 62회 KSBMB Annual Meeting in 2005 Vol.- No.-