신경망을 이용한 공정 오차 보정

전경아,유지용,오혜근 한양대학교 이학기술연구소 2002 이학기술연구지 Vol.5 No.-

패턴의 미세화에 따른 정확하고 신속한 결과 예측은 Iithography simulation tool 의 높은 공정 변수 의존을 요구한다. 이로 인해 simulation tool 은 공정 변수 사이의 보정이 더욱 복잡해져 광 근접 효과 등으로 나타나는 다양한 패턴들의 크기 결과를 예측하는데 많은 어려움을 겪게된다. 이에 본 논문에서는 simulation 결과 값과 실측값의 차이를 신경망 알고리즘에 적용하여 공정 변수를 최적함으로서 simulation tool의 예측 정확성을 향상시키는 방법을 제시하였다. It is required to make an accurate and quick prediction of critical dimension (CD)for higher integrated devices. Because simulation tools consist of many process parameters and models, it is difficult that process parameters are calibrated to match the CD results for various patterns. This paper presents a method of improving the accuracy of predicting CD results by applying △ (the difference between simulation and experimental data) value to neural network algorithm (NNA) in order to reduce the CD difference caused by optical proximity effects.

HrSOD-폴리에칠렌 접합체의 아세트아미노펜 간독성에 미치는 영향

용철순,박경아,오두만 영남대학교 약품개발연구소 1996 영남대학교 약품개발연구소 연구업적집 Vol.6 No.-

The covalent conjugation of human recombinant superoxide dismutase (hrSOD) with trichloros-triazine activated polyethylene glycol (PEG) 5000 formed soluble conjugates with molecular weight of 92KD, which retained 90~98% of original activity with a markedly prolonged plasma half-life of enzyme activity. The effect of hrSOD-PEG conjugates on acetaminophen (ACP)-in-duced hepatotoxicity was tested in male rats which were pretreated with 3-methylcholanthrene. HrSOD-PEG conjugates inhibited the hepatotoxicity produced by ACP, on the other hand, native hrSOD had no protective effect. The above results indicated that oxygen radicals might participate in the mechanism of the ACP-induced hepatotoxicity and that polymer conjugated-protein drugs with prolonged half-lives could be employed as an effective therapeutic agent.

실험적 진폐증에서 투여약제에 따른 면역학적 병리학적 조직변화

윤임중,임현우,노영만,오상용,정장영,임영,김경아 가톨릭 대학 산업의학 쎈타 산업의학연구소 1993 韓國의 産業醫學 Vol.32 No.2

To access the effects of some drugs such as piperazine phosphare, azathioprine and allopurinol in the experimental pneumoconiosis, 263 rats weighted 240-320 gm was divided into the control and eight experimental groups. To each group, the turbid solution mixed free silica, talc and natural coal dust of 50 mg in 0.8 ml saline were instilled intratracheally. At the begining and 5th week of experiment, piperazine phosphate(8mg/week orally), aspirin(1.5mg/day intramuscularly), azathioprine(25mg/day orally) and allopurinol(1.5mg/day orally) were administered simultaneously, while the dust turbid solution was only given to the control group. For each group the change of body weight. dry right lung weight, cellularity including total cell, macrophage, lymphocyte and neurtrophil in bronchoalveolar lavage fluid, superoxide ion, hydroxyproline, leukotriene B₄, tumor necrotsis factor, prostaglandin E₂were examined, and observed the pathological chages in lung tissue. The results were as follow : 1. The larger amount of their instilled dust and the longer observation period, the severer pathological findings were found in the lung tissue of each experimental group. 2. The body and dry right lung wight was not significantly changed in the experimental groups compared with the control group. 3. The number of total cell in bronchoalveolar lavage fluid was markedly less in the experimental group than the control one, while no significant difference was found in the percent alveolar macrophages and lymphocytes in both groups. However, the percent of neutrophiles was usually lower than in the control group. 4. The amount of superoxide ion was frequently less in the experimental group compared with the control one. 5. The amount of hydroxyproline was significantly lower in the experimental group than in the control one. 6. The amount of leukotriene B₄was usually less in the drug administered group than in the control one. 7. The amount of tumor necrotic factor was also frequently lower in each experimental group than in the control one. 8. The amount of prostaglandin E₂was usually higher in each experimental group than in the control one.

Refractory Ceramic Fiber와 Rock Wool로 자극한 폐포대식 세포에서 Reactive Oxygen Species 분비와 관련된 세포내 신호변환계

이권행,임영,양경숙,오현숙,김은경,김경아 大韓産業醫學會 1999 대한직업환경의학회지 Vol.11 No.4

Objectives : It is known that the high fibrogenecity of particles is connected with their cytotoxicity for macrophages. Although the molecular mechanism leading to fiber-induced fiber-induced cytotoxicity is still not clear, several mechanism have been suggested. The release of reactive oxygen species(ROS) from activated alveolar macrophages(AM) by dust have been suggested as a possible mechanism of particle-induced cell damage. But the mechanism which man-made vitreous fiber(MMVF) induces the production of ROS in AM is still not clear. In this study, we evaluated the relationship between ROS production and lactate dehydrogenase(LDH) release from alveolar treated with refractory ceramic fiber(RF2) or rock wool(RWI) and signal transduction path-way of ROS production in RF2 or RW1 exposed AM. Methods : We investigated LDH release from MMVF-stimulated AM for index of cytotoxicity. To determine what kind of signal transduction pathways are involved in MMVF-stimulated ROS generation, we used some drugs which have an effect on the signal transduction pathway. Results : RF2 and RW1 induced increase of LDH release with dose-dependent manner with RF2 having greater effect than RW1. There was a dose-dependent increase in the production of ROS by RF2 or RW1. At all level of concentration, RF2 induced more ROS production than RW1. Inhibitors of PKC(bisindolylmaleimide), PLC(U73122 and neomycine) and PTK(genistein and erbstatin) suppressed RF2 or RW1-induced ROS production. Conclusion : There was significant correlation between LDH release and ROS production from AM treated with RF2 or RW1. RF2 and RW1 induced ROS generation through protein kinase C(PKC), phospholipase C(PLC) and protein tyrosin kinase(PTK) pathways.

광물성 섬유의 수산이온기 생성이 흰쥐 폐포대식세포의 세포독성과 적혈구 세포막의 지질과산화에 미치는 영향

김경아,윤임중,김지홍,장황신,오민화,임영,박정일 大韓産業醫學會 1997 대한직업환경의학회지 Vol.9 No.3

There is some evidence suggesting that the mechanism of pulmonary toxicity of natural fibrous silicate, asbestos, is related to the generation of oxygen-based free radical. Especially the hydroxyl radical(·OH) is believed to be the most reactive one. ·OH is a potent toxic oxidant, ·OH is which means highly cytotoxic, genotoxic and carcinogenic. Nevertheless there has been no previous report about ·OH generation from man-made mineral fibers except asbestos. In this paper we tested the hypothesis that both cytotoxicity of alveolar macrophage and lipid peroxidation of erythrocytes are associated with ·OH production by mineral fibers in vitro. The results were as follows: 1. ·OH production in vitro was progressively increased by the concentration dependent pattern with the same mineral fiber. The production of ·OH in vitro by man-made mineral fiber was markedly decreased compared with that of natural mineral fiber except rock wool. 2. Lipid peroxidation of mineral fiber-exposed erythrocyte suspension was significantly increased compared with that of phosphate-buffered saline(PBS) control group. Lipid peroxidation in amosite and chrysotile group was larger than that of other mineral fibers. 3. There was a significantly positive correlation between ·OH production in vitro and lipid peroxidation of mineral fiber-exposed erythrocyte. 4. All of mineral fibers were related to the cytotoxicity of alveolar macrophage in concentration-dependent fashion. Chrysotile and amosite were more cytotoxic than crocidolite. Among man-made mineral fibers, ceramic fiber was the most cytotoxic to alveolar macrophage. The viability of alveolar macrophage exposed to asbestos was markedly decreased compared with that of man-made mineral fiver at the same concentration. 5. There was the significant negative correlation between the production of .OH and the viability of alveolar macrophages. From these results, we can conclude that the production of ·OH in vitro was strongly correlated with the cytotoxicity of alveolar macrophage and the lipid peroxidation in mineral fiber-exposed erythrocyte. And we documented that man-made mineral fiber could generate .OH in vitro less than asbestos.

Clinical and Radiological Features of Korean Patients With Anti-HMGCR Myopathy

Eun Kyoung Oh,Seung-Ah Lee,Hyun Joon Lee,Yoon Jin Cha,Sungjun Kim,Hyung-Soo Lee,Bum Chun Suh,Ha Young Shin,Seung Woo Kim,Byeol-A Yoon,Seong-il Oh,Yoo Hwan Kim,Joong-Yang Cho,Jeong Hee Cho,Ki-Han Kwon 대한신경과학회 2023 Journal of Clinical Neurology Vol.19 No.5

Background and Purpose To understand the characteristics of Korean patients with anti- 3-hydroxy-3-methylglutaryl-coenxyme A reductase (HMGCR) myopathy, we measured anti- HMGCR antibodies and analyzed the clinical, radiological, and pathological features of patients with anti-HMGCR myopathy. Methods We measured titers of anti-HMGCR antibodies in the sera of 99 patients with inflammatory myopathy, 36 patients with genetic myopathy, and 63 healthy subjects using an enzyme-linked immunosorbent assay. We tested 16 myositis-specific autoantibodies (MSAs) in all patients with anti-HMGCR myopathy. Results Positivity for the anti-HMGCR antibody was observed in 17 (4 males and 13 females) of 99 patients with inflammatory myopathy. The median age at symptom onset was 60 years. Ten (59%) of the patients with anti-HMGCR positivity had taken statins. The titer of anti- HMGCR antibodies was significantly higher in the statin-naïve group (median=230 U/mL, interquartile range=170–443 U/mL) than in the statin-exposed group (median=178 U/mL, interquartile range=105–210 U/mL, p=0.045). The most common symptom was proximal muscle weakness in 15 patients (88%), followed by myalgia in 9 (53%), neck weakness in 4 (24%), dysphagia in 3 (18%), and skin lesions in 2 (12%). The median titer of anti-HMGCR antibody was 202 U/mL. We found eight different MSAs in nine (53%) patients. The median disease duration from symptom onset to diagnosis was significantly shorter in the MSA-positive group than in the MSA-negative group (p=0.027). Conclusions Our study was the first to measure anti-HMGCR antibodies in inflammatory myopathy. It has provided new findings, including the suggestion of the coexistence of other MSAs in Korean patients.

S-365 : Comparison of the Haas and the Oxford Classifications for Prediction in IgA Nephropathy

( Kyoung Sook Park ),( Jeong Hae Kie ),( Ki Heon Nam ),( Mi Jung Lee ),( Beom Jin Lim ),( Young Eun Kwon ),( Yung Ly Kim ),( Seong Yeong An ),( Ah Young Ji ),( Min Hwan Kim ),( Hyung Jung Oh ),( Seung 대한내과학회 2013 대한내과학회 추계학술대회 Vol.2013 No.1

Background: Pathologic features can provide valuable information for determinining prognosis in IgA nephropathy (IgAN). However, it is uncertain whether the Oxford classification, a new classification of IgAN, can predict renal outcome better than previous ones. Method: We conducted a retrospective cohort study in 500 patients with biopsy-proven IgAN between January 2002 and December 2010 to compare the ability of the Haas and the Oxford classifications to predict renal outcome. Primary outcome was a doubling of the baseline serum creatinine concentration (D-SCr). Results: During a mean follow-up of 68 months, 52 (10.4%) and 35 (7.0%) developed D-SCr and ESRD, respectively. There were graded increases in the development of D-SCr in the higher Haas classes. In addition, the primary endpoint of D-SCr occurred more in patients with the Oxford M and T lesions than those without such lesions. In multivariable Cox regression analyses, the Haas class V (HR, 12.19; p=0.002) and the Oxford T1 (HR, 6.68; p<0.001) and T2 (HR, 12.16; p<0.001) lesions were independently associated with an increased risk of reaching D-SCr. Harrell`s C index of each multivariable model with the Haas and the Oxford classification was 0.867 (p=0.015) and 0.881 (p=0.004), respectively. This was significantly higher than that of model with clinical factors only (c-statistic=0.819). However, there was no difference in c-statistics between the two models with the Haas and the Oxford classifications (p=0.348). Conclusion: This study suggests that the Hass and the Oxford classifications are comparable in predicting progression of IgAN.

Construction of heterologous gene expression cassettes for the development of recombinant Clostridium beijerinckii

Oh, Young Hoon,Eom, Gyeong Tae,Kang, Kyoung Hee,Joo, Jeong Chan,Jang, Young-Ah,Choi, Jae Woo,Song, Bong Keun,Lee, Seung Hwan,Park, Si Jae Springer-Verlag 2016 BIOPROCESS AND BIOSYSTEMS ENGINEERING Vol.39 No.4

<P>Gene-expression cassettes for the construction of recombinant Clostridium beijerinckii were developed as potential tools for metabolic engineering of C. beijerinckii. Gene expression cassettes containing ColE1 origin and pAMB origin along with the erythromycin resistance gene were constructed, in which promoters from Escherichia coli, Lactococcus lactis, Ralstonia eutropha, C. acetobutylicum, and C. beijerinckii are examined as potential promoters in C. beijerinckii. Zymogram analysis of the cell extracts and comparison of lipase activities of the recombinant C. beijerinckii strains expressing Pseudomonas fluorescens tliA gene suggested that the tliA gene was functionally expressed by all the examined promoters with different expression level. Also, recombinant C. beijerinckii expressing C. beijerinckii secondary alcohol dehydrogenase by the constructed expression cassettes successfully produced 2-propanol from glucose. The best promoter for TliA expression was the R. eutropha phaP promoter while that for 2-propanol production was the putative C. beijerinckii pta promoter. Gene expression cassettes developed in this study may be useful tools for the construction of recombinant C. beijerinckii strains as host strains for the valuable chemicals and fuels from renewable resources.</P>

Timing of Adjuvant Chemotherapy after Curative Surgery for Colorectal Cancer

Kyoung Tae Noh,Boyoung Oh,Ryung-Ah Lee,Soon Sup Chung,Kwang Ho Kim 대한종양외과학회 2011 대한임상종양학회 학술대회지 Vol.2011 No.9

소아환자의 마취 후 예상하지 못했던 소아중환자실 입실

오아영,김경옥,송진석,김희수,박종두,김성덕,김종성 대한마취과학회 2002 Korean Journal of Anesthesiology Vol.43 No.3