Donepezil, Tacrine and α-Phenyl-n-tert-Butyl Nitrone (PBN) Inhibit Choline Transport by Conditionally Immortalized Rat Brain Capillary Endothelial Cell Lines (TR-BBB)

Young-Sook Kang,Kyeong-Eun Lee,Na-Young Lee,Tetsuya Terasaki 대한약학회 2005 Archives of Pharmacal Research Vol.28 No.4

In the present study, we have characterized the choline transport system and examined the influence of various amine drugs on the choline transporter using a conditionally immortalized rat brain capillary endothelial cell line (TR-BBB) in vitro. The cell-to-medium (C/M) ratio of [3H]choline in TR-BBB cells increased time-dependently. The initial uptake rate of [3H]choline was concentration-dependent with a Michaelis-Menten value, Km, of 26.2 ± 2.7 µM. The [3H]choline uptake into TR-BBB was Na+-independent, but was membrane potential-dependent. The [3H]choline uptake was susceptible to inhibition by hemicholinium-3, and tetraethylammonium (TEA), which are organic cation transporter substrates. Also, the uptake of [3H]choline was competitively inhibited with Ki values of 274 µM, 251 µM and 180 µM in the presence of donepezil hydrochloride, tacrine and α-phenyl-n-tert-butyl nitrone (PBN), respectively. These characteristics of choline transport are consistent with those of the organic cation transporter (OCT). OCT2 mRNA was expressed in TR-BBB cells, while the expression of OCT3 or choline transporter (CHT) was not detected. Accordingly, these results suggest that OCT2 is a candidate for choline transport at the BBB and may influence the BBB permeability of amine drugs.

둥시 장아찌 제조 과정 중 이화학적 특성 변화

차원섭,백신경,나경민,박준희,오상룡,이원영,천성숙,최웅규,조영제 한국응용생명화학회 2003 Applied Biological Chemistry (Appl Biol Chem) Vol.46 No.4

떫은감 품종의 하나인 등시를 이용하여 감장아찌 제조 중 과육의 이화학적 변화를 조사한 결과, 염도 변화는 저농도 간장 및 된장 침지의 경우 다소 완만한 증가를 보였고, 고농도의 간장 및 된장 침지의 경우 침지 기간내내 증가하는 것으로 나타났다. 간장 침지액 감장아찌의 경도는 간장 및 된장 모두 20-80% 농도에서는 침지 초기에는 증가하다가 침지 기간이 경과할수록 감소하는 경향을 나타내었다. 간장 침지 감장아찌의 색도 변화는 저장기간이 경과할수록 명도가 낮아지는 경향이었고, 적색도와 황색도는 침지액 농도를 달리하였을 경우 농도가 낮을수록, 침지 기간이 길어질수록 높았으며, 침지액 농도에 따른 차이는 크지 않았다. 침지액의 간장 및 된장의 농도를 달리하였을 때 감장아찌 중의 탄닌함량의 변화는 모든 침지액 농도에서 침지 기간이 지속될수록 탄닌의 함량은 저장 초기에 서서히 감소되다가 침지 기간이 길어질수록 급격히 감소하였으며, 첨가량에 따른 탄닌함량의 변화폭은 크지 않았다. 침지액의 간장 및 된장의 농도를 달리하였을 때 감과육에 존재하는 연화효소인 polygalacturonase와 pectinesterase 활성 변화는 간장침지 및 된장침지 모두 침지 초기부터 효소활성이 증가하기 시작하였으며 침지 기간이 경화할수록 효소활성은 더욱 높아지는 것을 알 수 있었고, 침지액의 농도가 높아질수록 효소활성이 억제되었다. Changes of physicochemical properties during the preparation of persimmon pickles were investigated. The salinity in persimmon pickles increased during storage time (0~50 days) with soy sauce and soy paste. When the fermented soy sauce and soy paste was added to the soaking solution with 80~100%, the salinity increased more rapidly. The hardness of persimmon pickles with soy sauce and soy paste was slightly increased up to the 20 or 30th day of storage and then decreased. L value of persimmon pickles was gradually decreased, but a and b value were slightly increased. The concentration of soluble tannin in persimmon pickles was slightly decreased down to the 20th day of storage and then decreased rapidly. The activities of polygalacturonase and pectinesterase as softening enzyme in persimmon pickles with soy sauce and soy paste increased during storage time (0-50 days) and enzyme activity was inhibited by high concentration of soaking solution.

Cnidium officinale, Fungal disease, Fusarium solani, Fusarium wilt

Na-Kyeong Kim,Hyo-Jeong Lee,Tae-Ho Ryu,In-Sook Cho,Ho-Jong Ju,Rae-Dong Jeong 한국식물병리학회 2021 식물병연구 Vol.27 No.2

The aim of the present study was to develop a sensitive and specific detection method for the rapid detec- tion of apple scar skin viroid (ASSVd) in apple leaves. The resulting reverse transcription recombinase poly- merase amplification (RT-RPA) assay can be completed in 10 min at 42oC, is 10 times more sensitive than con- ventional reverse transcription polymerase chain reaction, and can specifically amplify ASSVd without any cross-reactivity with other common apple viruses, including apple stem grooving virus, apple stem pitting virus, and apple chlorotic leaf spot virus. The reliability of the RT-RPA assay was assessed, and the findings suggested that it can be successfully utilized to detect ASSVd in field-collected samples. The RT-RPA assay developed in the present study provides a potentially valuable means for improving the detection of ASSVd in viroid-free certification programs, especially in resource-limited conditions.

ANTIALLERGIC EFFECT OF RHUS VERNICIFLUA IN RBL-2H3 CELLS

Kyeong Su Hong,Chun Soo Na,Young In Park,Mi-Sook Dong 한국환경독성학회 2009 한국독성학회 심포지움 및 학술발표회 Vol.2009 No.11

The Antioxidant Activity of Aster glehni by Extraction Methods

Kyeong-A Jang,Su-Koung Lee,Da-Bin Lee,Han-Na Chu,Eun-Suk Jung,Min-Sook Kang,Haeng-Ran Kim 한국식품영양과학회 2022 한국식품영양과학회 학술대회발표집 Vol.2022 No.10

Seed Morphology of Vitis (Vitaceae) in Korea

Kyeong Min Kim,Cho Hui Bak,Un Seop Shin,Da Hyun Lee,Do Hyun Kim,Geon Hui Ryu,Jong Sook Lee,Chae Sun Na 한국원예학회 2023 한국원예학회 학술발표요지 Vol.2023 No.5

Study on the Distribution of Carbapenem-Resistant Enterobacteriaceae (CRE) in Busan, 2022

Kyeong-A Kim,Hye-sook Heo,Hye-jun Kim,Su-Jin Shin,Young-Ran Na 대한미생물학회 2023 Journal of Bacteriology and Virology Vol.53 No.2

This study was conducted on the incidence of as carbapenemase-resistant Entero-bacteriaceae (CRE) infection and carbapenemase-producing Enterobacteriaceae(CPE) gene distribution in Busan to prepare basic data for preventing the spreadof CRE infection by confirming the regional characteristics of CRE infection. Thetotal of 2,429 strains from 92 clinics were obtained from rectal swab, sputum,stool, urine, blood, wound, bile, transtracheal aspiration, pus, ascetic fluid, bronchialwashing, catheter tip, genital, skin, sore, tissue, from January to December 2022. The CRE infection was tested by the method tested by the Korea Disease Controland Prevention Agency (KDCA). Species identification was confirmed by VITEK 2. Klebsiella pneumoniae which was detected in 1,698 (70.2%), was the mostcommon isolated CRE, followed by Escherichia coli in 430 (17.8%), Enterobactercloacae 90 (3.7%) and Citrobacter freundii 44 (1.8%). Among the identifiedCREs, 2,029 (83.5%) strains were identified as CPE. More than half of the detectedCPE types were 1,680 (69.2%) of KPC-2 (Klebsiella pneumonia carbapenases-2),followed by 166 (6.8%) of NDM-1 (New Delhi metallo-β-lactamase-1), 58 (2.4%)of KPC-3, and 51 (2.4%) of NDM-5. These findings provide good basic data forcomprehensive surveillance of CREs, suggesting that KPC and NDM are widespreadin Busan. In the analyzed CRE samples, Ertapenem resistance was the most com-mon at 99.5%, imipenem resistance 72.3%, meropenem resistance 67.9%, anddoripenem resistance 48.7%. It is expected that this study can be used as basicdata for preparing suitable countermeasures against CRE infection in the com-munity in the future.

위점막내 Helicobacter pylori 검사방법의 상호비교에 관한 연구

김나영(Na Young Kim),김경영(Kyeong Young Kim),김지영(Ji Young Kim),전호춘(Ho Cheun Jeon),차성은(Sung Eun Cha),이계희(Kye Heui Lee),구명숙(Myoung Sook Koo),최신은(Shin Eun Choi) 대한내과학회 1994 대한내과학회지 Vol.47 No.2

N/A Objectives: Many studies about the role of H. pylori on the gastritis, duodenal ulcer, benign gastric ulcer and stomach cancer have been carried out actively and many test methods of detecting H. Pylori have been developed. However, few studies have been reported about the sensitivity or applicability of these test methods and it can be seen that little consideration was taken into the sensitivity of these methods in some papers. This study was done to evaluate the sensitivity of each test method for identifying H. pylori infection and to evaluate time distribution of positive response in the CLO test. Methods: Four different test methods for identifying H. Pylori infection - Gram stain of touch print, CLO test, culture, H&E stain were taken in the 280 patients with duodenal ulcer. The sensitivity of each test was evaluated for the 264 H. pylari positive cases and positive time in the CLO test was measured. Results: 1) All four tests were positive in 80 cases (30.3%), three tests or more were positive in 197 cases (74.7%), and two tests or more were positive in 241 cases (91.3%). 2) There were 23 cases (8.7%) indicating H. pylori positive according to only one test, of which 15 cases (5.7%) were positive according to Gram stain and 8 cases (3.0%) according to CLO test, Either Gram stain or CLO test was positive in 100% of 264 H. pylori positive cases. 3) The sensitivity of Gram stain of touch print was 94.7%, that of CLO test 90.5% and that of H&E stain 69.3%, which was significantly lower than that of Gram stain or CLO test. The sensitivity of culture was 40.2% and it was significantly lower than that of H4E stain. 4) The positivie rate of Gram stain in the 106 culture positive cases was 100% (106 cases) and that of CLO test and H8rE stain were 93.4% (99 cases) and 77.4% (82 cases), respectively. 5) Of the 239 positive CLO tests, 129 cases (54.0%) indicated H. pylori positive within 20 min, 204 cases (85.4%), 231 cases (96.7%) and 239 cases (100%) within 1 hr, 3 hrs and 24 hrs, respectively. 6) Of the 99 culture and CLO test positive cases, 62 cases (62.6%) indicated H. pylori positive within 20 min in CLO test, and 87 cases (87.9%), 93 cases (93.95%) and 99 cases (100%) within 1 hr, 3 hrs and 24 hrs, respectively. Conclusion: From these results, we can conclude that the most sensitive test in detecting H. pylori is the Gram stain of touch print, and the next is CLO test. Just these two tests enable us to determine H. Pylori infection for almost all patients, this signifies that these two tests, not all of the four tests, are sufficient for H. pylori infection test. In the CLO test, 96.7% of the positive cases were diagnosed to be positive within 3 hrs, so that CLO test is considered to be a very speedy test in the diagnosis of H. pylori infection.

PHOSPHOINOSITIDE - SPECIFIC PHOSPHOLIPASE C OF PIG PANCREAS

Hye Kyeong Na,Yong Sook Kim,Deok Young Jhon 생화학분자생물학회 1993 생화학분자생물학회 춘계학술발표논문집 Vol.- No.-

공동통학구형 ‘어울림학교’ 운영에 따른 작은 학교의 변화

나종민(Na Jong Min),김천기(Kim Cheon Gie),임순일(Lym Soon Il),권미경(Kweon Mi Kyeong),박은숙(Park Eun Sook),이주은(Lee Ju Eun) 한독교육학회 2016 교육의 이론과 실천 Vol.21 No.3

본 연구의 목적은 전라북도교육청에서 2013년부터 추진하고 있는 어울림학교의 4가지 유형 중 선행연구가 없는 공동통학구형 어울림학교 운영에 따른 작은 학교의 변화를 살펴보고, 드러난 문제점을 해결할 수 있는 개선방안을 탐색하고자 한다. 이를 위해 전라북도에 근무하는 공동통학구형 어울림학교및 공동학구 학교에 근무하는 초등교원을 대상으로 심층면담을 실시하였다. 연구결과는 다음과 같다. (1) 공동통학구형 어울림학교 운영에 따른 외형적인 변화로는, 공동통학구형 어울림학교를 운영한 다수의 농촌지역 작은 학교의 전입 학생 수가 증가하였다는 것이다. 학생 수가 포화상태에 이른 A초 사례를 중심으로 살펴보면, 통폐합 위기의 농촌지역 작은 학교가 다음의 요인, 즉 공동학구 학교와의 거리, 지속적인 학교혁신, 학교관리자의 역량, 교육협력자로서 학부모의 역할 등에 의해 작고 좋은 학교로 성장하게 되었다. 또한 작고 좋은 학교를 유지하기 위해 학급당 학생 수를 제한함으로써 교육의 질을 관리하게 되었다. (2) 공동통학구형 어울림학교의 교육적 측면에서의 변화로는 첫째, 특성화된 교육 프로그램을 운영하였고, 교육과정이 내실화되었으며 둘째, 다인수학급에서 잘 적응하지 못 하거나 정서적 상처가 있어 많은 관심을 필요로 하는 학생들이 긍정적으로 변화했다는 점이다. 이런 학생의 변화에 있어 결정적 요인은 특별한 교육프로그램이 아닌 작은 학교 자체가 가진 특별한 교육환경이다. (3) 공동통학구형 어울림학교 운영을 개선하기 위해서는 첫째, 원거리에 위치하고 있는 농촌 작은 학교의 장애요인을 극복하고 교육적 잠재적 가능성을 펼칠 수 있도록 자생력을 가질 때까지 보다 장기적으로 통학차량 지원이 이루어져야 할 필요가 있다. 둘째, 공동통학구형 어울림학교로 전입해 오는 학생들로 인해 발생하는 어려움을 해결하기 위한 제도적인 맞춤형 교육 지원이 필요하다. 셋째, 전라북도교육청은 부정적인 학교 낙인효과를 예방하기 위해 작은 학교가 가진 교육경쟁력을 적극적으로 홍보할 방안을 마련해야 한다. 넷째, 공동학구 학교에 대한 적절한 지원은 학생 수를 놓고 경쟁관계에 위치한 공동학구 학교를 협력관계의 위치로 전환하는데 기여할 수 있을 것이다. The purpose of this study is to examine the changes and problems that the common attendance unit ‘Eoullim Schools’ without prior learning has made and explore how we can improve the operation of the school. The study had in-depth interviews of teachers who teach at common attendance unit‘Eoullim Schools’ and common district schools in Chonbuk. (1) What changes did common attendance unit ‘Eoullim Schools’ make in the small schools of the rural areas? The number of students in most of the common attendance unit ‘Eoullim Schools’ has increased. After investigating the case of ‘A’ Elementary School, we found that small schools facing a crisis of merger and abolition of the rural area became a small and good school by 5 success factors (distance to school, continuous innovation, competences of school managers and supportive parents), and specialized education programs and substantial curriculum). The school also improved the quality of education by limiting number of students in each class. (2) What significance does common attendance unit ‘Eoullim Schools’ have in education? First, specialized education programs and substantial curriculum. Second, Common attendance unit ‘Eoullim Schools’ has produced positive changes to students who had failed to adjust to large class or students who had emotional trauma. Important factor to the changes of students was not a special program, but the special educational environment that the small school provided. (3) What were the problems that common attendance unit ‘Eoullim Schools’ had and how can we solve them? First, in order to solve the problem that the schools are located in long distances, long-term support of school buses are necessary. Second, customized educational supporting programs should be provided to new students transferred to common attendance unit ‘Eoullim Schools’ who might have difficulties. Third, the Chonbuk Education Office should actively promote the positive aspects of ‘Eoullim Schools’ in order to prevent labelling effect. Fourth, proper support to common district schools will turn the schools’competition into cooperation.