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Development of salt tolerant soybean by marker assisted selection.
Jeonghwa Kim,Jong-Hyun Chae,Ji-Ho Park,Minsu Kim,Asekova Sovetgul,Jeong-Dong Lee 한국육종학회 2014 한국육종학회 심포지엄 Vol.2014 No.07
A total area of reclaimed land in Korea is about 135,100 ha, which occupies 9 % of total arable land. Soybean is one of the most important crop in Korea and demand for the crop is increasing, while the country’s self-sufficiency is very low, around 9 %. If it’s possible to cultivate soybean in reclaimed land, it would increase self-sufficiency of the soybean. However, there are difficulties to cultivate soybean in reclaimed land because of excessive level of salinity in the soil, to prevent this barrier in saline soils, it is necessary to develop salt tolerant soybean cultivar. This research was conducted to select salt tolerant lines derived from PI 483463 (salt tolerant wild soybean accession). The F1 (Hutcheson × PI 483463) was backcrossed with Hutcheson and Wooram (salt sensitive soybean cultivar). For marker assisted selection and salt reaction phenotyping, randomly selected BC1F1 seeds from two backcross populations were planted in 11 cm tall tray. At the V1 growth stage, DNA was sampled with FTA card. The genomic DNA and SSR marker, BARCSOYSSR_03_1348, were used for PCR amplification and the result was checked through electrophoresis. The trays with BC1F1 plants were immersed in 100 mM NaCl solution up to the bottom third of the trays directly after the DNA extraction. After two weeks, phenotype was measured depending on leaf scorch degree. Through this research, 25 dominant homozygote lines and 22 heterozygote lines from Hutcheson backcross population and 28 dominant homozygote lines and 37 heterozygote lines from Wooram backcross population were selected. These lines will be used for developing soybean with salt tolerance
Jeonghwa Ko,Gayeon Gu,Yoonah Kim,Soyeon Park,Sujin Park,Jiyu Seo,Hojun Jang,Ayeon Joo,Juhang Kim 한국성인간호학회 2021 성인간호학회 학술대회 Vol.2021 No.8
Aim(s): There is a lack of research that has produced results on the relationship between nursing college students' nursing process performance, critical thinking disposition, and nursing information literacy competency. This study seeks to understand the impact of nursing college students' critical thinking disposition and nursing information literacy competency on nursing process performance. Method(s): The subjects of study are 180 students from the Department of Nursing at K University in E providence. Data collection took place from 17 August to 2 September 2020. The research tools used critical thinking disposition, NILC-N (Nursing Information Literacy Competency) tools, and nursing process performance tools. The collected data analysis used the SPSS/WIN 25.0 program, using technical statistics and Independent t-test, ANOVA, Pearson’s correlation coefficient method. Result(s): Critical thinking disposition is whether or not nursing courses are completed (121.79±16.28 vs 119.31±21.52, t=.842, p=.028) showed a difference depending on. Nursing information literacy competency differed between the first and second groups(83.93±16.15) and the third and fourth grade groups(99.04±12.18). Nursing information literacy competency differed depending on whether or not clinical practice was experienced(99.12±12.09 vs 83.65±16.09, t=7.177, p=.047) and Completion of nursing informatics course (65.78±9.57 vs 49.48±16.38, t=5.501, p=.015) showed differences. The study subjects’ nursing process performance showed a positive correlation between critical thinking disposition(r=.413, p<.001) and nursing information literacy competency(r=.769, p<.001). Conclusion(s): It was confirmed that the impact on nursing college students’ ability to nursing process performance was critical thinking disposition and nursing information literacy competency. In order to improve nursing college students' nursing process performance, it is necessary to develop educational programs that can foster nursing information literacy competency and critical thinking levels.
혈관내피세포 탈착에 미치는 melatonin의 병리학적 영향
Jeonghwa Seo(서정화),Sunghyen Kim(김성현),Sunyoung Ahn(안선영),Eunsil Jeong(정은실),Jingu Cho(조진구),Heonyong Park(박헌용) 한국생명과학회 2010 생명과학회지 Vol.20 No.6
항산화 기능과 면역 억제 기능을 갖는 것으로 알려진 melatonin이 혈관 내피층에서는 어떤 기능을 갖는지 알기 위한 일련의 실험을 수행하였다. 본 연구의 실험 결과, 혈관기능과 관련된 혈관내피세포의 성장, 사멸, 이동에 melatonin은 특이적인 효과를 나타내지 않았고, 백혈구의 혈관내피세포 부착과 백혈구 동종간의 응집에도 melatonin의 역할이 관찰되지 않았다. 이와는 대조적으로 melatonin은 PP2A를 통해 eNOS의 활성을 억제하여 산화질소의 양을 감소시키고, 이로 인해 혈관내피세포의 탈착이 유발되는 효과가 있음을 확인할 수 있었다. 종합해보면, 혈액 내 고농도의 melatonin은 PP2A 및 eNOS의 활성을 변화시켜 혈관내피세포의 탈착을 상승시킴으로써 혈관내에서 발생할 수 있는 혈전 형성에 의한 병리적 현상을 유발할 수 있다. In this study, we carried out a series of experiments to know whether melatonin, an anti-oxidative and immunosuppressive agent, played an important role in endothelial cells. It was revealed that melatonin had little or no effect on endothelial proliferation, cell death or migration. Additionally, melatonin had no effect on adhesion of THP-1 leukocytes to bovine aortic endothelial cells (BAECs) and THP-1 homotypic cell aggregation. In contrast, it was shown that melatonin diminished the basal level of nitric oxide by PP2A-mediated dephosphorylation of endothelial nitric oxide synthase (eNOS), leading to enhanced detachment of BAEC from the extracellular matrix. Collectively, melatonin in high doses decreases the NO production via regulations of PP2A and eNOS activities, inducing detachment of endothelial cells, a possible initial step for thrombosis.
서정화(Jeonghwa Seo),김정민(Jeongmin Kim),안선영(Sunyoung Ahn),조진구(Jingu Cho),김종민(Jongmin Kim),박헌용(Heonyong Park) 한국생명과학회 2009 생명과학회지 Vol.19 No.7
고대부터 식품으로 많이 이용되어 왔던 마늘의 성분중의 하나인 alliin의 혈관 생리활성을 조사하기 위해 다양한 실험을 수행하였다. Alliin은 혈관내피세포의 증식과 이동을 증진시키는 기능이 있으며, 이는 alliin이 혈관형성을 촉진하고 혈관의 상처 치유에 도움을 줄 수 있음을 의미한다. 또한 alliin은 염증반응을 일으키는 과정에 나타나는 THP-1 세포의 혈관내 피세포 부착을 억제하며, 혈전을 형성하는 THP-1 동종세포간 응집을 억제하는 기능도 갖고 있음이 확인되었다. 이와 같은 alliin의 세포 기능은 혈관의 주요 질환인 동맥경화의 발생 및 뇌졸증이나 심근경색의 원인이 되는 혈전의 형성 등을 억제할 수 있음을 의미한다. 한편 혈관의 주요 조절자 중의 하나인 산화질소의 생산에는 alliin이 관여하지 않음을 확인하였다. 종합해 보면, alliin은 혈관세포의 여러 가지 생리기능을 조절하는 혈관생리기능 개선제로 활용할 가능성이 있는 물질이다. Little is known about the cardiovascular roles of alliin, a functional component in garlic that has been used as food material. Thus, we examined a broad range of cardiovascular activities of alliin in this study. From our in vitro experiments, alliin was determined to act as a stimulant to induce endothelial cell proliferation and endothelial cell migration. Since endothelial cell proliferation and migration are highly associated with angiogenesis and wound healing, alliin is suggested as a regulator to control angiogenesis and wound healing. In addition, alliin was elucidated to prevent lipopolysaccharide (LPS)-induced adhesion of THP-1 leukocytes to endothelial cells and LPS-induced homotypic THP-1 cell aggregation. These inhibitory effects indicate that alliin is likely to act as an anti-atherosclerotic and anti-thrombotic factor, because leukocytic adhesion to endothelial cells and homotypic leukocyte aggregation are highly associated with atherosclerosis and thrombosis, respectively. Our additional findings show that alliin has no effect on the production of nitric oxide (NO), an important vasoregulator. In conclusion, alliin is suggested as a regulator for controlling various cardiovascular functions.