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Kim Ye Seul,Yoon Jung Won,Kim Dasol,Choi Seunghak,Kim Hyoung Kyu,Youm Jae Boum,Han Jin,Heo Soon Chul,Hyun Sung-Ae,Seo Jung-Wook,Kim Deok-Ho,Kim Jae Ho 생화학분자생물학회 2022 Experimental and molecular medicine Vol.54 No.-
Human embryonic stem cell-derived cardiomyocytes (hESC-CMs) have been reported to exhibit immature embryonic or fetal cardiomyocyte-like phenotypes. To enhance the maturation of hESC-CMs, we identified a natural steroidal alkaloid, tomatidine, as a new substance that stimulates the maturation of hESC-CMs. Treatment of human embryonic stem cells with tomatidine during cardiomyocyte differentiation stimulated the expression of several cardiomyocyte-specific markers and increased the density of T-tubules. Furthermore, tomatidine treatment augmented the number and size of mitochondria and enhanced the formation of mitochondrial lamellar cristae. Tomatidine treatment stimulated mitochondrial functions, including mitochondrial membrane potential, oxidative phosphorylation, and ATP production, in hESC-CMs. Tomatidine-treated hESC-CMs were more sensitive to doxorubicin-induced cardiotoxicity than the control cells. In conclusion, the present study suggests that tomatidine promotes the differentiation of stem cells to adult cardiomyocytes by accelerating mitochondrial biogenesis and maturation and that tomatidine-treated mature hESC-CMs can be used for cardiotoxicity screening and cardiac disease modeling.
Inhibition of Biofilm Formation and Hyphal Growth of Candida albicans by Medium-chain Fatty Acids
Ye Seul KIM,Afreen JAILANI,Jintae LEE 한국생물공학회 2021 한국생물공학회 학술대회 Vol.2021 No.10
C. albicans biofilms are tolerant of conventional antifungal therapeutics and the host immune system. The transition of yeast cells to hyphae is considered a key step in C. albicans biofilm development, and this transition is inhibited by the quorum sensing molecule farnesol. We hypothesized that fatty acids mimicking farnesol might influence hyphal and biofilm formation by C. albicans. Among 31 saturated and unsaturated fatty acids, six medium-chain saturated fatty acids, that is, heptanoic acid, octanoic acid, nonanoic acid, decanoic acid, undecanoic acid, and lauric acid, effectively inhibited C. albicans biofilm formation by more than 75% at 2 ㎍/ml. These six fatty acids at 2 ㎍/ml and farnesol at 100 ㎍/ml inhibited hyphal growth and cell aggregation. The addition of fatty acids to C. albicans culture decreased the productions of farnesol and sterols. Furthermore, the down-regulations of several hyphal and biofilm related genes caused by heptanoic or nonanoic acid closely resembled the changes caused by farnesol. In addition, the most active nonanoic acid diminished C. albicans virulence in a Caenorhabditis elegans model. Our results suggest that medium-chain fatty acids more effectively inhibit hyphal growth and biofilm formation than farnesol.
FC 2-7 : Elevated KAI1 protein expression identified in malignant melanoma
( Ye Seul Kim ),( Ji Hoon Sim ),( Sang Jin Oh ),( Je Min An ),( Sang Hoon Lee ),( Sung Yul Lee ),( Moon Kyun Cho ) 대한피부과학회 2013 대한피부과학회 학술발표대회집 Vol.65 No.2
Background: KAI1/CD82 reportedly slows the metastatic process in a variety of tumors. However, its function is not fully clear. This study investigates the expression of KAI1 in correlation with malignant skin cancers. Objectives: To investigate the expression and clinical significance of KAI1 protein in malignant skin cancers. Methods: KAI1/CD82 expression was evaluated by western blotting in 24 specimens including 6 malignant melanoma (MM), 6 squamous cell carcinoma (SCC), 6 BCC and 6 normal skin specimens. Immunohistochemical staining was performed to measure KAI1/CD82 expression in 6 MM, 6 SCC and 6 BCC. Results: We observed over-expression of KAI1 in the malignant melanoma. The expressions of KAI1 in malignant melanoma were higher than those in SCC and BCC. The KAI1 expressions in malignant melanoma were higher in protein levels compared to SCC and BCC. Western blotting results agreed with immunohistochemistry. Conclusion: KAI1 is highly related to malignant melanoma and may be a useful indicator of malignant melanoma.