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Ha, Jang-Ho,Kim, Ha-Neul,Moon, Ki-Beom,Jeon, Jae-Heung,Jung, Dai-Hyun,Kim, Su-Jung,Mason, Hugh,Shin, Seo-Yeon,Kim, Hyun-Soon,Park, Kyung-Mok Georg Thieme Verlag KG 2017 Planta medica Vol.83 No.10
<B>Abstract</B><P>Responding to the need for recombinant acidic fibroblast growth factor in the pharmaceutical and cosmetic industries, we established a scalable expression system for recombinant human aFGF using transient and a DNA replicon vector expression in Nicotiana benthamiana. Recombinant human-acidic fibroblast growth factor was recovered following Agrobacterium infiltration of N. benthamiana. The optimal time point at which to harvest recombinant human acidic fibroblast growth factor expressing leaves was found to be 4 days post-infiltration, before necrosis was evident. Commassie-stained SDS-PAGE gels of His-tag column eluates, concentrated using a 10 000 molecular weight cut-off column, showed an intense band at the expected molecular weight for recombinant human acidic fibroblast growth factor. An immunoblot confirmed that this band was recombinant human acidic fibroblast growth factor. Up to 10 µg recombinant human-acidic fibroblast growth factor/g of fresh leaves were achieved by a simple affinity purification protocol using protein extract from the leaves of agroinfiltrated N. benthamiana. The purified recombinant human acidic fibroblast growth factor improved the survival rate of UVB-irradiated HaCaT and CCD-986sk cells approximately 89 and 81 %, respectively. N. benthamiana-derived recombinant human acidic fibroblast growth factor showed similar effects on skin cell proliferation and UVB protection compared to those of Escherichia coli-derived recombinant human acidic fibroblast growth factor. Additionally, N. benthamiana-derived recombinant human acidic fibroblast growth factor increased type 1 procollagen synthesis up to 30 % as well as reduced UVB-induced intracellular reactive oxygen species generation in fibroblast (CCD-986sk) cells.</P><P>UVB is a well-known factor that causes various types of skin damage and premature aging. Therefore, the present study demonstrated that N. benthamiana-derived recombinant human acidic fibroblast growth factor effectively protects skin cell from UVB, suggesting its potential use as a cosmetic or therapeutic agent against skin photoaging.</P>
S-700 Achaete-scute complex homologue 2 accelerates Sjogren-like diseases in the NO D/ShiLtJ mouse
( Ha-neul Kim ),( Sung-min Kim ),( Jin-sil Park ),( Mi-la Cho ),( Sung-hwan Park ) 대한내과학회 2016 대한내과학회 추계학술대회 Vol.2016 No.1
Ascl2 has been reported to induce the differentiation of TFH cells and the activation of TFH cells, which are essential for development of Sjogren's syndrome (SS). This study tested Ascl2 to determine its role in the development of SS. NOD/ShiLtJ mice were injected with the overexpression vector of Ascl2 and the infiltration of lymphocytes into salivary or lacrimal glands. Inflammatory cytokines or chemo-attractive -factor for T or B cells were also determined. The activation of TFH cells were tested using the specific marker of TFH cells. Ascl2 level was also tested in SS patients. CXCR5 were increased by the administration of Ascl2 (p<0.0001) in both salivary and lacrimal glands. Pro-inflammatory cytokines and chemo-attractive factor such as IL-6, TNF-α, IL-8, PD-1, IL-21 and Bcl-6 were also increased by Ascl2 (p<0.03 and p<0.01). TFH cells activation was elucidated via enlargement of T cell zone and the populations of CD4+CXCR5+, CD4+ICOS+, CD4+PD-1+ cells also increased by Ascl2 expression. Ascl2 upregulates the TFH cell-specific markers and activates TFH cells through chemo-attractive factors such as IL-8, CXCR5, PD-1, IL-21 and Bcl-6. Ascl2 also increased the pro-inflammatory cytokines TNF-α and IL-6. IL-21 and Bcl-6 as well as Ascl2 in peripheral blood mononuclear cells are dramatically increased in SS patient, which confirmed Ascl2 is a potential pathognomonic factor in SS patient.
Crystal structure and functional characterization of SF216 from <i>Shigella flexneri</i>
Kim, Ha-Neul,Seok, Seung-Hyeon,Lee, Yoo-Sup,Won, Hyung-Sik,Seo, Min-Duk Wiley (John WileySons) 2017 FEBS letters Vol.591 No.21
<P>Shigella flexneri is a Gram-negative anaerobic bacterium that causes highly infectious bacterial dysentery in humans. Here, we solved the crystal structure of SF216, a hypothetical protein from the S.flexneri 5a strain M90T, at 1.7 angstrom resolution. The crystal structure of SF216 represents a homotrimer stabilized by intersubunit interactions and ion-mediated electrostatic interactions. Each subunit consists of three beta-strands and five alpha-helices with the beta-beta-beta-alpha-alpha-alpha-alpha-alpha topology. Based on the structural information, we also demonstrate that SF216 shows weak ribonuclease activity by a fluorescence quenching assay. Furthermore, we identify potential druggable pockets (putative hot spots) on the surface of the SF216 structure by computational mapping.</P>
CT Findings of Azygos Venous System: Congenital Variants and Acquired Structural Changes
Ha Neul Kim,Youkyung Lee,Su Jin Hong,Ji Hun Kang,Ji-hoon Jung 대한영상의학회 2024 대한영상의학회지 Vol.85 No.1
The azygos venous system is a crucial conduit of the posterior thorax and potentially vital collateral pathway. However, it is often overlooked clinically and radiologically. This pictorial essay reviews the normal azygos venous anatomy and CT findings of congenital variations and structural changes associated with acquired pathologies.
Cordyceps militaris alleviates non-alcoholic fatty liver disease in ob/ob mice
Ha-Neul Choi,Yang-Hee Jang,Min-Joo Kim,Min Jeong Seo,Byoung Won Kang,Yong Kee Jeong3,,Jung-In Kim 대한지역사회영양학회 2014 Nutrition Research and Practice Vol.8 No.2
BACKGROUND/OBJECTIVES: Non-alcoholic fatty liver disease (NAFLD) is becoming an important public health problem as metabolic syndrome and type 2 diabetes have become epidemic. In this study we investigated the protective effect of Cordyceps militaris (C. militaris) against NAFLD in an obese mouse model. MATERIALS/METHODS: Four-week-old male ob/ob mice were fed an AIN-93G diet or a diet containing 1% C. militaris water extract for 10 weeks after 1 week of adaptation. Serum glucose, insulin, free fatty acid (FFA), alanine transaminase (ALT), and proinflammatory cytokines were measured. Hepatic levels of lipids, glutathione (GSH), and lipid peroxide were determined. RESULTS: Consumption of C. militaris significantly decreased serum glucose, as well as homeostasis model assessment for insulin resistance (HOMA-IR), in ob/ob mice. In addition to lowering serum FFA levels, C. militaris also significantly decreased hepatic total lipids and triglyceride contents. Serum ALT activities and tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) levels were reduced by C. militaris. Consumption of C. militaris increased hepatic GSH and reduced lipid peroxide levels. CONCLUSIONS: These results indicate that C. militaris can exert protective effects against development of NAFLD, partly by reducing inflammatory cytokines and improving hepatic antioxidant status in ob/ob mice.
The catalytic role of additive components for the nitridation of silicon/additive mixture
KIM, Ha-Neul,PARK, Young-Jo,KIM, Jin-Myung,LEE, Jae-Wook,KO, Jae-Woong,KIM, Hai-Doo,LEE, Seung-Jun,BAEK, Seung-Su,SEO, Il-Sung Ceramic Society of Japan 2016 JOURNAL OF THE CERAMIC SOCIETY OF JAPAN - Vol.124 No.3
<P>The nitriding behavior of silicon/additives mixture with varied composition was investigated by focusing on the catalytic effect of sintering additives (Y2O3 and Al2O3) and diluents (Si3N4). While sintering additives have originally been mixed to form a liquid phase to promote both densification and grain growth during post-sintering, it was revealed that the addition of Y2O3 enhances the nitriding reaction by eliminating the SiO2 and Si3N4 layer on the surface of silicon particles. Further, a synergistic effect resulting in an increased nitridation was verified by concurrent addition of sintering additives and diluents. The contribution of each component of the compact to the promoted nitriding reaction was elucidated in quasi in-situ manner by the combination of phase analysis and thermogravimetric analysis. (C) 2016 The Ceramic Society of Japan. All rights reserved.</P>
Effects of microstructure and intergranular glassy phases on thermal conductivity of silicon nitride
Kim, Jin-Myung,Ko, Sin-Il,Kim, Ha-Neul,Ko, Jae-Woong,Lee, Jae-Wook,Kim, Hai-Doo,Park, Young-Jo Elsevier 2017 CERAMICS INTERNATIONAL Vol.43 No.7
<P><B>Abstract</B></P> <P>In this study, the binary sintering additives Y<SUB>2</SUB>O<SUB>3</SUB>-Sc<SUB>2</SUB>O<SUB>3</SUB>, were first applied to the Si<SUB>3</SUB>N<SUB>4</SUB> system to investigate their effects on microstructure and thermal conductivity. The microstructure and thermal conductivity of both sintered silicon nitride (SSN) and sintered reaction-bonded silicon nitride (SRBSN) were found to be significantly dependent on the additive composition. Among various combinations of Y<SUB>2</SUB>O<SUB>3</SUB> and Sc<SUB>2</SUB>O<SUB>3</SUB>, 1mol% Y<SUB>2</SUB>O<SUB>3</SUB>−3mol% Sc<SUB>2</SUB>O<SUB>3</SUB> prominently enhanced thermal conductivity, and the enhancement could not be attributed to any difference in microstructure or lattice defects. TEM observation revealed that this composition was more liable to devitrify the glassy phase with a lower degree of stress accumulation, and to possibly produce a grain boundary that was cleaner or with a higher order of atomic arrangement. A microstructure model for thermal conductivity was proposed which took the thermal resistance of the grain boundaries into account. The grain boundary state exerted a remarkable influence on the thermal conductivity of fine microstructures, and the experimentally measured thermal conductivity values were consistent with those given by the proposed model.</P>